Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,088,662 C→A 8.4% intergenic (+158/‑96) pmbA → / → PP_0943 regulatory protease/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,088,6620CA 8.4% 183.4 / 5.7 59intergenic (+158/‑96)pmbA/PP_0943regulatory protease/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (27/27);  new base A (2/3);  total (29/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-01

CCTTTCGCGGGTAAACCCGCTCCTACACGGTGGTGGACGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGTTCATGAAATCCGTCCTCCGCGAACTGCCCTACCTGGAAAACTGGCGCTGGC  >  NC_002947/1088523‑1088806
                                                                                                                                           |                                                                                                                                                
ccTTTCGCGGGTAAACCCGCTCCTACACGGAGGTGGACGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTAAACCTTTCCCTGAAAATCCGAAGACCCGGCCCTCGCGCCGGGt                                                                                                                                         >  4:278905/1‑149 (MQ=255)
 cTTTCGCGGGTAAACCCGCTCCTACACGGTGGTGGACGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCGCGAGGGCCGGGtc                                                                                                                                        <  1:93794/149‑2 (MQ=255)
     cgcgGGTAAACCCGCTCCTACACGGTGGTGGACGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGttttt                                                                                                                                     <  3:338858/148‑1 (MQ=255)
       cgGGTAAACCCGCTCCTACACGGTGGTGGACGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTAtt                                                                                                                                  <  2:187811/149‑1 (MQ=255)
        gggTAAACCCGCTCCTACACGGTGGTGGACGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTAtt                                                                                                                                  >  6:194705/1‑148 (MQ=255)
             aaCCCGCTCCTACACGGTGGTGGACGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGTACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTtcatca                                                                                                                            <  1:35348/149‑1 (MQ=255)
             aaCCCGCTCCTACACGGTGGTGGACGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTtcatca                                                                                                                            >  6:297260/1‑149 (MQ=255)
              aCCCGCTCCTACACGGTGGTGGACGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCAc                                                                                                                           >  2:321836/1‑149 (MQ=255)
                ccGCTCCTACACGGTGGTGGACGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCt                                                                                                                         >  7:286018/1‑149 (MQ=255)
                    tCCTACACGGTGGTGGACGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCAACtttt                                                                                                                      >  1:340976/1‑148 (MQ=255)
                      cTACACGGTGGTGGACGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTc                                                                                                                    <  5:297260/148‑1 (MQ=255)
                         cacGGTGGTGGACGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCtt                                                                                                                  <  5:153700/147‑1 (MQ=255)
                            ggtggtGGACGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGt                                                                                                             <  8:212139/149‑1 (MQ=255)
                              tggtggACGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGa                                                                                                           <  3:335885/149‑1 (MQ=255)
                                gtggACGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGAtt                                                                                                         <  8:286018/149‑1 (MQ=255)
                                 tggACGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTc                                                                                                        >  3:292153/1‑149 (MQ=255)
                                   gACGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTa                                                                                                      <  4:62267/149‑1 (MQ=255)
                                   gACGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTa                                                                                                      >  3:276112/1‑149 (MQ=255)
                                     cGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTAtt                                                                                                    <  8:192376/149‑1 (MQ=255)
                                      gTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCGCGAGGGCCGGGtctt                                                                                                                                      >  8:64549/1‑114 (MQ=25)
                                      gTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCGCGAGGGCCGGGtctt                                                                                                                                      <  7:64549/114‑1 (MQ=25)
                                      gTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTAtt                                                                                                    >  5:69624/1‑148 (MQ=255)
                                           tCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCAc                                                                                              >  4:47291/1‑149 (MQ=255)
                                              gggTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACtt                                                                                            >  5:280803/1‑148 (MQ=255)
                                                     acacAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCtt                                                                                                                  >  2:64281/1‑119 (MQ=255)
                                                     acacAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCtt                                                                                                                  <  1:64281/119‑1 (MQ=255)
                                                          ggCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGAGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATc                                                                               <  3:47291/149‑1 (MQ=255)
                                                           gCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACATCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCa                                                                              <  3:9368/149‑1 (MQ=255)
                                                           gCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCa                                                                              <  6:69624/149‑1 (MQ=255)
                                                           gCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCa                                                                              <  4:292153/149‑1 (MQ=255)
                                                                 gTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATc                                                                         <  1:321836/148‑1 (MQ=255)
                                                                  tAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATc                                                                         >  1:178838/1‑147 (MQ=255)
                                                                     gAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGt                                                                     >  3:217480/1‑148 (MQ=255)
                                                                               aCCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCgat                                                          <  4:217480/149‑1 (MQ=255)
                                                                                 ccGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCgatga                                                        >  5:62296/1‑149 (MQ=255)
                                                                                   gcgAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGatgatg                                                      <  4:276112/149‑1 (MQ=255)
                                                                                    cgAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGt                                                     >  1:317784/1‑149 (MQ=255)
                                                                                      aaGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGtt                                                    >  1:80110/1‑148 (MQ=255)
                                                                                       agagGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGTTCa                                                  <  5:194705/149‑1 (MQ=255)
                                                                                           gCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGTTCATGa                                               >  1:203336/1‑148 (MQ=255)
                                                                                               gAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGTTCATGAAATcc                                          <  6:280803/149‑1 (MQ=255)
                                                                                                aaCAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGTTCATGAAATCCg                                         <  2:80110/149‑1 (MQ=255)
                                                                                                   aGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAata                                                                                 >  2:27314/1‑106 (MQ=255)
                                                                                                     cACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTAttt                                                                                                   <  6:349489/86‑1 (MQ=255)
                                                                                                     cACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTAttt                                                                                                   >  5:349489/1‑86 (MQ=255)
                                                                                                             cTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGTTc                                                   <  6:56172/126‑1 (MQ=255)
                                                                                                             cTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGTTc                                                   >  5:56172/1‑126 (MQ=255)
                                                                                                             cTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGTTCATGAAATCCGtcctcc                                   >  7:189720/1‑142 (MQ=255)
                                                                                                             cTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGTTCATGAAATCCGtcctcc                                   <  8:189720/142‑1 (MQ=255)
                                                                                                             cTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGTTCATGAAATCCGTCCTCCGCGaa                              <  6:238242/147‑1 (MQ=255)
                                                                                                             cTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGTTCATGAAATCCGTCCTCCGCGaa                              >  5:238242/1‑147 (MQ=255)
                                                                                                                   cTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGTTCATGAAATCCGTCCTCCGCGAACTGCCCTa                      >  2:217720/1‑149 (MQ=255)
                                                                                                                   cTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGTTCATGAAATCCGTCCTCCGCGAACTGCCCTa                      >  3:3071/1‑149 (MQ=255)
                                                                                                                      cAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGTTCATGAAATCCGTCCTCCGCGAACTGCCCTACCt                   <  2:317784/149‑1 (MQ=255)
                                                                                                                           ataaaaaCCCGGCGCGAGGGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGTTCATGAAATCCGTCCTCAGCGAACTGCCCTACCTGGaa               >  2:219388/7‑148 (MQ=255)
                                                                                                                             aaaaaCCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGTTCATGAAATCCGTCCTCCGCGAACTGCCCTACCTGGAAAAc            >  2:263171/5‑149 (MQ=255)
                                                                                                                               aaaCCCGGCGCGAGGGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGTTCATGAAATCCGTCCTCCGCGAACTGCCCTACCTGGAAAACTg          <  1:219388/147‑1 (MQ=255)
                                                                                                                                        ccTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGTTCATGAAATCCGTCCTCCGCGAACTGCCCTACCGGGAAAACTGGCGCTGGc  <  2:113579/148‑1 (MQ=255)
                                                                                                                                        ccTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGTTCATGAAATCCGTCCTCCGCGAACTGCCCTACCGGGAAAACTGGCGCTGGc  >  1:113579/1‑148 (MQ=255)
                                                                                                                                           |                                                                                                                                                
CCTTTCGCGGGTAAACCCGCTCCTACACGGTGGTGGACGTACATCGGGGTTGGACACAGGCCGTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGAACAGCACTACACCTTTCCCTGCAAATCCGAAGACCCGGCCCTCGCGCCGGGTTTTTATTTCATCACCTTTTTCTTGAAGTGATTCTATTTATCACTTAATAATGAATATCATTATCCAGTAACCCGGCGATGATGTTCATGAAATCCGTCCTCCGCGAACTGCCCTACCTGGAAAACTGGCGCTGGC  >  NC_002947/1088523‑1088806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑