Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,101,873 A→G 6.9% K152E (AAG→GAG)  mlaD → phospholipid ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,101,8730AG 6.9% 165.2 / 2.3 58K152E (AAG→GAG) mlaDphospholipid ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (23/31);  new base G (2/2);  total (25/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

CCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTGGCCTGCTGGTGCTGCTGGCGGCCTTCCCCCTGCTGGCCCTGGCTGTGCAGACACCGCACGAAGTGGTTCAAAGCACCACCAAT  >  NC_002947/1101727‑1102019
                                                                                                                                                  |                                                                                                                                                  
ccgcggGTTTGCTTGTTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAATGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAATTTCCTGCTCAACTCTGTTGGCAAg                                                                                                                                                  <  7:169921/149‑1 (MQ=255)
   ccgGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGaa                                                                                                                                               <  7:120628/149‑1 (MQ=255)
   ccgGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGaa                                                                                                                                               >  2:220175/1‑149 (MQ=255)
      gTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAAcc                                                                                                                                             >  8:143876/1‑148 (MQ=255)
       tttGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGa                                                                                                                                           <  2:76231/149‑1 (MQ=255)
            ttGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAaagaa                                                                                                                                      <  7:300701/149‑1 (MQ=255)
              ggTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGATGTTCTCAAGGATTGTGTTTCCATCGATGACACACAGTCGGCTCTGGTGGTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAtagaag                                                                                                                                     <  2:239573/148‑1 (MQ=255)
                   gaAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACATACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAAc                                                                                                                               <  1:260783/149‑1 (MQ=255)
                   gaAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAAc                                                                                                                               <  7:158995/149‑1 (MQ=255)
                       tACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGc                                                                                                                           <  7:143876/149‑1 (MQ=255)
                        aCATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGc                                                                                                                           >  2:123839/1‑148 (MQ=255)
                           tCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAAt                                                                                                                       >  3:224528/1‑149 (MQ=255)
                               cATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAAGAACCGAAAGAAGCGCAACCGGCTAATTAAg                                                                                                                   <  7:327815/149‑1 (MQ=255)
                                 tCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGa                                                                                                                 <  4:224528/149‑1 (MQ=255)
                                 tCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGa                                                                                                                 <  1:123839/149‑1 (MQ=255)
                                    gCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTa                                                                                                              >  7:166018/1‑149 (MQ=255)
                                                  gACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGa                                                                                                                                           >  3:157003/1‑106 (MQ=255)
                                                  gACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGa                                                                                                                                           <  4:157003/106‑1 (MQ=255)
                                                           cTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGAc                                                                                        <  4:60687/148‑1 (MQ=255)
                                                            tCATGGATGGTTGCACCATCCTTGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGt                                                                                      >  6:7749/1‑149 (MQ=255)
                                                            tCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGTAAcc                                                                                                                                             >  6:15880/1‑94 (MQ=255)
                                                            tCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAAcc                                                                                                                                             <  5:15880/94‑1 (MQ=255)
                                                            tCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGa                                                                                                                 <  2:260937/122‑1 (MQ=255)
                                                              aaGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTgg                                                                                    >  5:308771/1‑149 (MQ=255)
                                                                 gATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTGGCct                                                                                 >  5:237973/1‑149 (MQ=255)
                                                                       gCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTGGCCTGCTGGt                                                                           >  2:284979/1‑149 (MQ=255)
                                                                       gCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGGGGCCTGCTGGt                                                                           >  8:293269/1‑149 (MQ=255)
                                                                       gCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATACTGCGACGTGGCCTGCTGGt                                                                           >  1:94591/1‑149 (MQ=255)
                                                                        cAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTGGCCTGCTGGt                                                                           <  8:166018/148‑1 (MQ=255)
                                                                         aaCCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAg                                                                                                                   <  1:142354/107‑1 (MQ=255)
                                                                         aaCCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAg                                                                                                                   >  2:142354/1‑107 (MQ=255)
                                                                         aaCCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTGGCCTGCTGGtgc                                                                         <  1:220175/149‑1 (MQ=255)
                                                                                 atGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTGGCCTGCTGGTGCTGCTggcg                                                                 <  6:237973/149‑1 (MQ=255)
                                                                                        acaGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGa                                                                                                                 >  2:324541/1‑94 (MQ=255)
                                                                                        acaGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGa                                                                                                                 <  1:324541/94‑1 (MQ=255)
                                                                                        acaGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTGGCCTGCTGGTGCTGCTGGCGGCCTTcc                                                          >  5:40420/1‑149 (MQ=255)
                                                                                          aGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTGGCCTGCTGGTGCTGCTGGCGGCCTTcccc                                                        <  6:308771/149‑1 (MQ=255)
                                                                                           gTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGCCAAGGAACCGAAAGACGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTGGCCTGCTGGTGCTGCTGGCGGCCTTccccc                                                       <  1:284979/149‑1 (MQ=255)
                                                                                               gCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGAttt                                                                                                    <  4:131504/100‑1 (MQ=255)
                                                                                               gCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGAttt                                                                                                    >  3:131504/1‑100 (MQ=255)
                                                                                                cACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTAAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTGGCCTGCTGGTGCTGATGGCGGCCTTCCCCctgctg                                                  >  3:79782/1‑149 (MQ=255)
                                                                                                        cTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTGGCCTGCTGGTGCTGCTGGCGGCCTTCCCCCTGCTGGCCCTgg                                           <  2:94591/148‑1 (MQ=255)
                                                                                                           gaggaTCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTGGCCTGCTGGTGCTGCTGGCGGCCTTCCCCCTGCTGGCCCTGGCtgt                                       <  6:40420/149‑1 (MQ=255)
                                                                                                                        gCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTGGCCTGCTGGTGCTGCTGGCGGCCTTCCCCCTGCTGGCCCTGGCTGTGCAGACACCGCAc                          >  4:155047/1‑149 (MQ=255)
                                                                                                                        gCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAACCGCAACCGGCTAAATAAGGAGTACCCATGATTTCGATCCTGCGACGTGGCCTGCTGGTGCTGCTGGCGGCCTTcccc                                                        <  2:125156/119‑1 (MQ=255)
                                                                                                                          aaGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGGGTACCCATGATTTCGATCCTGCGACGTGGCCTGCTGGt                                                                           <  8:145289/98‑1 (MQ=255)
                                                                                                                          aaGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGGGTACCCATGATTTCGATCCTGCGACGTGGCCTGCTGGt                                                                           >  7:145289/1‑98 (MQ=255)
                                                                                                                           aGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTGGCCTGCTGGTGCTGCTGGCGGCCTTCCCCCTGCTGGCCCTGGCTGTGCAGACACCGCACGaa                       <  3:155047/149‑1 (MQ=255)
                                                                                                                             ttCCTGCTCAACTCTGTTGGCGAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTGGCCTGCTGGTGCTGCTGGCGGCCTTCCCCCTGCTGGCCCTgg                                           >  7:278824/1‑127 (MQ=255)
                                                                                                                             ttCCTGCTCAACTCTGTTGGCGAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTGGCCTGCTGGTGCTGCTGGCGGCCTTCCCCCTGCTGGCCCTgg                                           <  8:278824/127‑1 (MQ=255)
                                                                                                                               ccTGCTCATGTGTGTTGGCAAGGAACGGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTGGATCCTGCGACGTGGCCTGCTGGTGCTGCTGGCGGCCTTCCCCATGCTGGCCCTGGCTGTGCAGACACCGCACGAAGTg                    <  4:79782/148‑1 (MQ=255)
                                                                                                                                cTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTGGCCTGCGGTTGCTGCTGGCGTCCTTCCCCCTGCTGGCCCTGGCTGTGCAGACACCGCACGAAGTgg                   <  1:262503/148‑1 (MQ=255)
                                                                                                                                 tGCTCAACTCTGTTGGCGAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTGGCCTGCTGGTGCTGCTGGCGGCCTTCCCCCTGCTGGCCCTGGCTGTGCAGACACCGCACGaa                       >  7:262904/1‑143 (MQ=255)
                                                                                                                                 tGCTCAACTCTGTTGGCGAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTGGCCTGCTGGTGCTGCTGGCGGCCTTCCCCCTGCTGGCCCTGGCTGTGCAGACACCGCACGaa                       <  8:262904/143‑1 (MQ=255)
                                                                                                                                      aaCTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTGGCCTGCTGGTGCTGCTGGCGGCCTTc                                                           <  2:120908/102‑1 (MQ=255)
                                                                                                                                      aaCTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTGGCCTGCTGGTGCTGCTGGCGGCCTTCCCCCTGCTGGCCCTGGCTGTGCAGACACCGCACGAAGTGGTTCAAAg            >  8:34481/1‑149 (MQ=255)
                                                                                                                                      aaCTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTGGCCTGCTGGTGCTGCTGGCGGCCTTCCCCCTGCTGGCCCTGGCTGTGCAGACACCGCACGAAGTGGTTCAAAg            >  2:219500/1‑149 (MQ=255)
                                                                                                                                                gCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTGGCCTGCTGGTGCTGCTGGCGGCCTTCCCCCTGCTGGCCCTGGCTGTGCAGACACCGCACGAAGTGGTTCAAAGCACCACCAAt  >  5:205287/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTGGCCTGCTGGTGCTGCTGGCGGCCTTCCCCCTGCTGGCCCTGGCTGTGCAGACACCGCACGAAGTGGTTCAAAGCACCACCAAT  >  NC_002947/1101727‑1102019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑