Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,115,371	G→T	7.6%	L821M (CTG→ATG)	valS ←	valine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,115,371	0	G	T	7.6%	207.4 / 6.3	66	L821M (CTG→ATG)	valS	valine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (29/32);  new base T (2/3);  total (31/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-01

GTCACCGACCAGGGCGGTAGCGGACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCATGATGGTC  >  NC_002947/1115225‑1115519                                                                                                                                                   |                                                                                                                                                     gTCACCGACCAGGGCGGTAGCGGACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAAcagcg                                                                                                                                                    >  3:113143/1‑149 (MQ=255)    aCCGACCAGGGCGGTAGCGGACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAAcatcggcc                                                                                                                                                 >  3:225660/1‑148 (MQ=255)    aCCGACCAGGGCGGTAGCGGACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGAAGCGGTTCGTTTTCGTGCAGGCGGCGCTGGTCGGCGGCGTGGGCGGGGTGCAGGAAcagcgg                                                                                                                                                   >  2:311261/1‑147 (MQ=255)     ccGACCAGGGCGGTAGCGGACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCCCTGGTCGTCGGCGATGGCGTACTTCAGGAAcagcggaag                                                                                                                                                >  6:57533/1‑149 (MQ=255)      cGACCAGGGCGGTAGCGGACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAAcagcggcagc                                                                                                                                               <  7:79946/149‑1 (MQ=255)         ccAGGGCGGTAGCGGACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACagcggcagcggc                                                                                                                                            >  7:163844/1‑149 (MQ=255)          cAGGGCGGTAGCGGACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGATCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACatcggccccggc                                                                                                                                            >  2:17700/1‑148 (MQ=255)             ggCGGTAGCGGACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGc                                                                                                                                        <  8:165284/149‑1 (MQ=255)                ggTAGCGGACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCgg                                                                                                                                     <  4:113143/149‑1 (MQ=255)                      ggACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGa                                                                                                                               >  7:37498/1‑149 (MQ=255)                      ggACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGa                                                                                                                               >  7:115045/1‑149 (MQ=255)                       gACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGAGCTTGGCCAGCTTCTTCTGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCTGGAACAGCGGCAGCGGCTTGCCGGGGCCGAt                                                                                                                              <  2:270753/149‑1 (MQ=255)                        aCAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGATCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACATCGGCCCCGGCAAGccgctgccgctg                                                                                                                             <  1:17700/149‑1 (MQ=255)                          aGCGGCGCTTCGTCGGCATCACCCAGTACAGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGtt                                                                                                                           >  4:210446/1‑149 (MQ=255)                            cggcgCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACATCGGCCCCGGCAAGCCGCTGCCGCTGTTcc                                                                                                                         <  4:225660/149‑2 (MQ=38)                              gcgcTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCgggg                                                                                                                                   >  4:195338/1‑137 (MQ=255)                              gcgcTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCgggg                                                                                                                                   <  3:195338/137‑1 (MQ=255)                              gcgcTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGGCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCAtt                                                                                                                       <  8:163844/149‑1 (MQ=255)                                   tcgtcgGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGgc                                                                                                                  >  7:69324/1‑149 (MQ=255)                                    cgtcgGCATCACCCAGTACAGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGgc                                                                                                                  <  3:210446/148‑1 (MQ=255)                                     gtcgGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGgcgc                                                                                                                <  8:69324/149‑1 (MQ=255)                                      tcgGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGcgcg                                                                                                               >  3:205029/1‑149 (MQ=255)                                         gCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGc                                                                                                                                  <  8:174675/127‑1 (MQ=255)                                            tCACCCAGTACGGTGAACGACTTGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAAcagcgg                                                                                                                                                   >  1:103555/1‑107 (MQ=255)                                            tCACCCAGTACGGTGAACGACTTGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAAcagcgg                                                                                                                                                   <  2:103555/107‑1 (MQ=255)                                            tCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACATCGGCCCCGGCAAGCCGCTGCCGCTGTTc                                                                                                                          >  3:228995/1‑132 (MQ=38)                                            tCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACATCGGCCCCGGCAAGCCGCTGCCGCTGTTc                                                                                                                          <  4:228995/132‑1 (MQ=38)                                            tCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTc                                                                                                                     <  3:307835/137‑1 (MQ=255)                                            tCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTc                                                                                                                     >  4:307835/1‑137 (MQ=255)                                                ccAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTgcgc                                                                                                     <  1:311261/149‑1 (MQ=255)                                                  aGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTc                                                                                                                          >  7:208284/1‑126 (MQ=255)                                                  aGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTc                                                                                                                          <  8:208284/126‑1 (MQ=255)                                                    tACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGc                                                                                                 <  8:37498/149‑1 (MQ=255)                                                       ggTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGa                                                                                              >  7:214241/1‑149 (MQ=255)                                                           aaCGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCa                                                                                           >  5:242935/1‑148 (MQ=255)                                                              gACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCaa                                                                                       <  3:152751/149‑1 (MQ=255)                                                                 tCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCG‑TTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAActct                                                                                   >  7:55369/1‑149 (MQ=255)                                                                   gACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCtt                                                                                  >  8:187144/1‑149 (MQ=255)                                                                    aCCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGAc                                                                                             <  3:17019/137‑1 (MQ=255)                                                                    aCCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGAc                                                                                             >  4:17019/1‑137 (MQ=255)                                                                     ccTTTGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTc                                                                                <  4:205029/149‑1 (MQ=255)                                                                      cTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCa                                                                               <  8:214241/149‑1 (MQ=255)                                                                          gCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCa                                                                           >  4:164010/1‑149 (MQ=255)                                                                          gCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCa                                                                           >  4:219394/1‑149 (MQ=255)                                                                           ccAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCAc                                                                          <  7:187144/149‑1 (MQ=255)                                                                            cAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACt                                                                         >  1:45662/1‑149 (MQ=255)                                                                             aGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCCGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTc                                                                        <  5:57533/149‑1 (MQ=255)                                                                              gCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCAt                                                                                          <  2:273326/130‑1 (MQ=255)                                                                              gCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCAt                                                                                          >  1:273326/1‑130 (MQ=255)                                                                               cttcttCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAg                                                                                                   <  7:232783/120‑1 (MQ=255)                                                                               cttcttCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAg                                                                                                   >  8:232783/1‑120 (MQ=255)                                                                                   ttcAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCa                                                                               >  1:48226/1‑136 (MQ=255)                                                                                   ttcAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCa                                                                               <  2:48226/136‑1 (MQ=255)                                                                                       gcagcGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTc                                                                                >  2:160856/1‑131 (MQ=255)                                                                                         agcGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTc                                                            <  5:33074/149‑1 (MQ=255)                                                                                             cTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCgg                                                          >  1:170307/1‑147 (MQ=255)                                                                                             cTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCa                                                        <  6:242935/149‑1 (MQ=255)                                                                                                  gtttCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAgcggc                                                   <  8:55369/148‑1 (MQ=255)                                                                                                   tttCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCg                                                           >  6:138783/1‑140 (MQ=255)                                                                                                   tttCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCg                                                           <  5:138783/140‑1 (MQ=255)                                                                                                       cTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGAc                                                                                             >  1:284768/1‑102 (MQ=255)                                                                                                       cTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGAc                                                                                             <  2:284768/102‑1 (MQ=255)                                                                                                        tGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCaa                                              >  5:203271/1‑148 (MQ=255)                                                                                                          cAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAAt                                             <  8:115045/147‑1 (MQ=255)                                                                                                              cggcgCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGc                                       <  3:219394/149‑1 (MQ=255)                                                                                                              cggcgCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGc                                       <  3:164010/149‑1 (MQ=255)                                                                                                                     ggTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTg                                >  2:243351/1‑149 (MQ=255)                                                                                                                         gtcgGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGt                                         <  7:65510/136‑1 (MQ=255)                                                                                                                         gtcgGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGt                                         >  8:65510/1‑136 (MQ=255)                                                                                                                          tcgGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAAt                                             <  8:86964/131‑1 (MQ=255)                                                                                                                          tcgGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAAt                                             >  7:86964/1‑131 (MQ=255)                                                                                                                                   gcgttCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATgg                  <  2:170307/149‑1 (MQ=255)                                                                                                                                         gaaatgAACATCGGCCCCGGCAAGCCGCTGCCGCTGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTgcag            >  5:361737/6‑149 (MQ=38)                                                                                                                                          tcAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTgcag            >  2:299957/1‑148 (MQ=255)                                                                                                                                             ggAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCat         >  1:199262/1‑148 (MQ=255)                                                                                                                                                   gcggcagcggcTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCATGATGGTc  <  6:203271/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                     GTCACCGACCAGGGCGGTAGCGGACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCATGATGGTC  >  NC_002947/1115225‑1115519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑