Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,115,377	G→C	7.4%	L819V (CTG→GTG) ‡	valS ←	valine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,115,377	0	G	C	7.4%	211.3 / 4.3	66	L819V (CTG→GTG) ‡	valS	valine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (29/32);  new base C (2/3);  total (31/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGACCAGGGCGGTAGCGGACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCATGATGGTCTTGCCG  >  NC_002947/1115229‑1115525                                                                                                                                                     |                                                                                                                                                     ccGACCAGGGCGGTAGCGGACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCCCTGGTCGTCGGCGATGGCGTACTTCAGGAAcagcggaag                                                                                                                                                      >  6:57533/1‑149 (MQ=255)  cGACCAGGGCGGTAGCGGACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAAcagcggcagc                                                                                                                                                     <  7:79946/149‑1 (MQ=255)     ccAGGGCGGTAGCGGACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACagcggcagcggc                                                                                                                                                  >  7:163844/1‑149 (MQ=255)      cAGGGCGGTAGCGGACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGATCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACatcggccccggc                                                                                                                                                  >  2:17700/1‑148 (MQ=255)         ggCGGTAGCGGACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGc                                                                                                                                              <  8:165284/149‑1 (MQ=255)            ggTAGCGGACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCgg                                                                                                                                           <  4:113143/149‑1 (MQ=255)                  ggACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGa                                                                                                                                     >  7:115045/1‑149 (MQ=255)                  ggACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGa                                                                                                                                     >  7:37498/1‑149 (MQ=255)                   gACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGAGCTTGGCCAGCTTCTTCTGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCTGGAACAGCGGCAGCGGCTTGCCGGGGCCGAt                                                                                                                                    <  2:270753/149‑1 (MQ=255)                    aCAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGATCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACATCGGCCCCGGCAAGccgctgccgctg                                                                                                                                   <  1:17700/149‑1 (MQ=255)                      aGCGGCGCTTCGTCGGCATCACCCAGTACAGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGtt                                                                                                                                 >  4:210446/1‑149 (MQ=255)                        cggcgCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACATCGGCCCCGGCAAGCCGCTGCCGCTGTTcc                                                                                                                               <  4:225660/149‑2 (MQ=38)                          gcgcTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCgggg                                                                                                                                         >  4:195338/1‑137 (MQ=255)                          gcgcTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCgggg                                                                                                                                         <  3:195338/137‑1 (MQ=255)                          gcgcTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGGCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCAtt                                                                                                                             <  8:163844/149‑1 (MQ=255)                               tcgtcgGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGgc                                                                                                                        >  7:69324/1‑149 (MQ=255)                                cgtcgGCATCACCCAGTACAGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGgc                                                                                                                        <  3:210446/148‑1 (MQ=255)                                 gtcgGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGgcgc                                                                                                                      <  8:69324/149‑1 (MQ=255)                                  tcgGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGcgcg                                                                                                                     >  3:205029/1‑149 (MQ=255)                                     gCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGc                                                                                                                                        <  8:174675/127‑1 (MQ=255)                                        tCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACATCGGCCCCGGCAAGCCGCTGCCGCTGTTc                                                                                                                                <  4:228995/132‑1 (MQ=38)                                        tCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACATCGGCCCCGGCAAGCCGCTGCCGCTGTTc                                                                                                                                >  3:228995/1‑132 (MQ=38)                                        tCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTc                                                                                                                           >  4:307835/1‑137 (MQ=255)                                        tCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTc                                                                                                                           <  3:307835/137‑1 (MQ=255)                                            ccAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTgcgc                                                                                                           <  1:311261/149‑1 (MQ=255)                                              aGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTc                                                                                                                                <  8:208284/126‑1 (MQ=255)                                              aGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTc                                                                                                                                >  7:208284/1‑126 (MQ=255)                                                tACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGc                                                                                                       <  8:37498/149‑1 (MQ=255)                                                   ggTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGa                                                                                                    >  7:214241/1‑149 (MQ=255)                                                       aaCGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCa                                                                                                 >  5:242935/1‑148 (MQ=255)                                                          gACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCaa                                                                                             <  3:152751/149‑1 (MQ=255)                                                             tCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCG‑TTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAActct                                                                                         >  7:55369/1‑149 (MQ=255)                                                               gACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCtt                                                                                        >  8:187144/1‑149 (MQ=255)                                                                aCCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGAc                                                                                                   >  4:17019/1‑137 (MQ=255)                                                                aCCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGAc                                                                                                   <  3:17019/137‑1 (MQ=255)                                                                 ccTTTGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTc                                                                                      <  4:205029/149‑1 (MQ=255)                                                                  cTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCa                                                                                     <  8:214241/149‑1 (MQ=255)                                                                      gCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCa                                                                                 >  4:219394/1‑149 (MQ=255)                                                                      gCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCa                                                                                 >  4:164010/1‑149 (MQ=255)                                                                       ccAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCAc                                                                                <  7:187144/149‑1 (MQ=255)                                                                        cAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACt                                                                               >  1:45662/1‑149 (MQ=255)                                                                         aGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCCGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTc                                                                              <  5:57533/149‑1 (MQ=255)                                                                          gCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCAt                                                                                                <  2:273326/130‑1 (MQ=255)                                                                          gCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCAt                                                                                                >  1:273326/1‑130 (MQ=255)                                                                           cttcttCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAg                                                                                                         >  8:232783/1‑120 (MQ=255)                                                                           cttcttCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAg                                                                                                         <  7:232783/120‑1 (MQ=255)                                                                               ttcAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCa                                                                                     >  1:48226/1‑136 (MQ=255)                                                                               ttcAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCa                                                                                     <  2:48226/136‑1 (MQ=255)                                                                                   gcagcGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTc                                                                                      >  2:160856/1‑131 (MQ=255)                                                                                     agcGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTc                                                                  <  5:33074/149‑1 (MQ=255)                                                                                         cTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCgg                                                                >  1:170307/1‑147 (MQ=255)                                                                                         cTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCa                                                              <  6:242935/149‑1 (MQ=255)                                                                                              gtttCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAgcggc                                                         <  8:55369/148‑1 (MQ=255)                                                                                               tttCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCg                                                                 >  6:138783/1‑140 (MQ=255)                                                                                               tttCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCg                                                                 <  5:138783/140‑1 (MQ=255)                                                                                                   cTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGAc                                                                                                   >  1:284768/1‑102 (MQ=255)                                                                                                   cTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGAc                                                                                                   <  2:284768/102‑1 (MQ=255)                                                                                                    tGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCaa                                                    >  5:203271/1‑148 (MQ=255)                                                                                                      cAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAAt                                                   <  8:115045/147‑1 (MQ=255)                                                                                                          cggcgCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGc                                             <  3:219394/149‑1 (MQ=255)                                                                                                          cggcgCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGc                                             <  3:164010/149‑1 (MQ=255)                                                                                                                 ggTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTg                                      >  2:243351/1‑149 (MQ=255)                                                                                                                     gtcgGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGt                                               <  7:65510/136‑1 (MQ=255)                                                                                                                     gtcgGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGt                                               >  8:65510/1‑136 (MQ=255)                                                                                                                      tcgGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAAt                                                   >  7:86964/1‑131 (MQ=255)                                                                                                                      tcgGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAAt                                                   <  8:86964/131‑1 (MQ=255)                                                                                                                               gcgttCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATgg                        <  2:170307/149‑1 (MQ=255)                                                                                                                                     gaaatgAACATCGGCCCCGGCAAGCCGCTGCCGCTGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTgcag                  >  5:361737/6‑149 (MQ=38)                                                                                                                                      tcAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTgcag                  >  2:299957/1‑148 (MQ=255)                                                                                                                                         ggAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCat               >  1:199262/1‑148 (MQ=255)                                                                                                                                               gcggcagcggcTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCATGATGGTc        <  6:203271/149‑1 (MQ=255)                                                                                                                                                cggcagcggcTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCATGATGGTCt       >  3:107982/1‑149 (MQ=255)                                                                                                                                                  gcagcggcTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCATGATGGTCTTg     <  1:299957/149‑1 (MQ=255)                                                                                                                                                     gcggcTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGGAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCATGATGGTCTTGCCg  >  4:133913/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                     CCGACCAGGGCGGTAGCGGACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCATGATGGTCTTGCCG  >  NC_002947/1115229‑1115525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑