Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,115,382	T→A	6.7%	K817M (AAG→ATG) ‡	valS ←	valine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,115,382	0	T	A	6.7%	220.1 / 2.7	71	K817M (AAG→ATG) ‡	valS	valine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (32/34);  new base A (2/3);  total (34/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGGTAGCGGACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCATGATGGTCTTGCCGTCGAT  >  NC_002947/1115237‑1115530                                                                                                                                                  |                                                                                                                                                     ggCGGTAGCGGACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGc                                                                                                                                                   <  8:165284/149‑1 (MQ=255)    ggTAGCGGACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCgg                                                                                                                                                <  4:113143/149‑1 (MQ=255)          ggACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGa                                                                                                                                          >  7:115045/1‑149 (MQ=255)          ggACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGa                                                                                                                                          >  7:37498/1‑149 (MQ=255)           gACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGAGCTTGGCCAGCTTCTTCTGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCTGGAACAGCGGCAGCGGCTTGCCGGGGCCGAt                                                                                                                                         <  2:270753/149‑1 (MQ=255)            aCAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGATCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACATCGGCCCCGGCAAGccgctgccgctg                                                                                                                                        <  1:17700/149‑1 (MQ=255)              aGCGGCGCTTCGTCGGCATCACCCAGTACAGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGtt                                                                                                                                      >  4:210446/1‑149 (MQ=255)                cggcgCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACATCGGCCCCGGCAAGCCGCTGCCGCTGTTcc                                                                                                                                    <  4:225660/149‑2 (MQ=38)                  gcgcTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCgggg                                                                                                                                              >  4:195338/1‑137 (MQ=255)                  gcgcTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCgggg                                                                                                                                              <  3:195338/137‑1 (MQ=255)                  gcgcTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGGCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCAtt                                                                                                                                  <  8:163844/149‑1 (MQ=255)                       tcgtcgGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGgc                                                                                                                             >  7:69324/1‑149 (MQ=255)                        cgtcgGCATCACCCAGTACAGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGgc                                                                                                                             <  3:210446/148‑1 (MQ=255)                         gtcgGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGgcgc                                                                                                                           <  8:69324/149‑1 (MQ=255)                          tcgGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGcgcg                                                                                                                          >  3:205029/1‑149 (MQ=255)                             gCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGc                                                                                                                                             <  8:174675/127‑1 (MQ=255)                                tCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACATCGGCCCCGGCAAGCCGCTGCCGCTGTTc                                                                                                                                     >  3:228995/1‑132 (MQ=38)                                tCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACATCGGCCCCGGCAAGCCGCTGCCGCTGTTc                                                                                                                                     <  4:228995/132‑1 (MQ=38)                                tCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTc                                                                                                                                >  4:307835/1‑137 (MQ=255)                                tCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTc                                                                                                                                <  3:307835/137‑1 (MQ=255)                                    ccAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTgcgc                                                                                                                <  1:311261/149‑1 (MQ=255)                                      aGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTc                                                                                                                                     >  7:208284/1‑126 (MQ=255)                                      aGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTc                                                                                                                                     <  8:208284/126‑1 (MQ=255)                                        tACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGc                                                                                                            <  8:37498/149‑1 (MQ=255)                                           ggTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGa                                                                                                         >  7:214241/1‑149 (MQ=255)                                               aaCGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCa                                                                                                      >  5:242935/1‑148 (MQ=255)                                                  gACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCaa                                                                                                  <  3:152751/149‑1 (MQ=255)                                                     tCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCG‑TTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAActct                                                                                              >  7:55369/1‑149 (MQ=255)                                                       gACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCtt                                                                                             >  8:187144/1‑149 (MQ=255)                                                        aCCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGAc                                                                                                        <  3:17019/137‑1 (MQ=255)                                                        aCCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGAc                                                                                                        >  4:17019/1‑137 (MQ=255)                                                         ccTTTGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTc                                                                                           <  4:205029/149‑1 (MQ=255)                                                          cTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCa                                                                                          <  8:214241/149‑1 (MQ=255)                                                              gCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCa                                                                                      >  4:219394/1‑149 (MQ=255)                                                              gCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCa                                                                                      >  4:164010/1‑149 (MQ=255)                                                               ccAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCAc                                                                                     <  7:187144/149‑1 (MQ=255)                                                                cAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACt                                                                                    >  1:45662/1‑149 (MQ=255)                                                                 aGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCCGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTc                                                                                   <  5:57533/149‑1 (MQ=255)                                                                  gCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCAt                                                                                                     <  2:273326/130‑1 (MQ=255)                                                                  gCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCAt                                                                                                     >  1:273326/1‑130 (MQ=255)                                                                   cttcttCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAg                                                                                                              >  8:232783/1‑120 (MQ=255)                                                                   cttcttCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAg                                                                                                              <  7:232783/120‑1 (MQ=255)                                                                       ttcAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCa                                                                                          >  1:48226/1‑136 (MQ=255)                                                                       ttcAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCa                                                                                          <  2:48226/136‑1 (MQ=255)                                                                           gcagcGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTc                                                                                           >  2:160856/1‑131 (MQ=255)                                                                             agcGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTc                                                                       <  5:33074/149‑1 (MQ=255)                                                                                 cTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCgg                                                                     >  1:170307/1‑147 (MQ=255)                                                                                 cTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCa                                                                   <  6:242935/149‑1 (MQ=255)                                                                                      gtttCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAgcggc                                                              <  8:55369/148‑1 (MQ=255)                                                                                       tttCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCg                                                                      <  5:138783/140‑1 (MQ=255)                                                                                       tttCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCg                                                                      >  6:138783/1‑140 (MQ=255)                                                                                           cTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGAc                                                                                                        >  1:284768/1‑102 (MQ=255)                                                                                           cTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGAc                                                                                                        <  2:284768/102‑1 (MQ=255)                                                                                            tGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCaa                                                         >  5:203271/1‑148 (MQ=255)                                                                                              cAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAAt                                                        <  8:115045/147‑1 (MQ=255)                                                                                                  cggcgCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGc                                                  <  3:164010/149‑1 (MQ=255)                                                                                                  cggcgCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGc                                                  <  3:219394/149‑1 (MQ=255)                                                                                                         ggTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTg                                           >  2:243351/1‑149 (MQ=255)                                                                                                             gtcgGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGt                                                    >  8:65510/1‑136 (MQ=255)                                                                                                             gtcgGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGt                                                    <  7:65510/136‑1 (MQ=255)                                                                                                              tcgGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAAt                                                        <  8:86964/131‑1 (MQ=255)                                                                                                              tcgGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAAt                                                        >  7:86964/1‑131 (MQ=255)                                                                                                                       gcgttCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATgg                             <  2:170307/149‑1 (MQ=255)                                                                                                                             gaaatgAACATCGGCCCCGGCAAGCCGCTGCCGCTGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTgcag                       >  5:361737/6‑149 (MQ=38)                                                                                                                              tcAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTgcag                       >  2:299957/1‑148 (MQ=255)                                                                                                                                 ggAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCat                    >  1:199262/1‑148 (MQ=255)                                                                                                                                       gcggcagcggcTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCATGATGGTc             <  6:203271/149‑1 (MQ=255)                                                                                                                                        cggcagcggcTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCATGATGGTCt            >  3:107982/1‑149 (MQ=255)                                                                                                                                          gcagcggcTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCATGATGGTCTTg          <  1:299957/149‑1 (MQ=255)                                                                                                                                             gcggcTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGGAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCATGATGGTCTTGCCg       >  4:133913/1‑149 (MQ=255)                                                                                                                                                gcTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCg                                                                                  >  2:72931/1‑71 (MQ=255)                                                                                                                                                  ttGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCATGATGGTCTTGCCGTCGAt  >  3:263463/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                     GGCGGTAGCGGACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCATGATGGTCTTGCCGTCGAT  >  NC_002947/1115237‑1115530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑