Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,115,388	G→C	5.9%	P815R (CCC→CGC)	valS ←	valine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,115,388	0	G	C	5.9%	218.1 / 3.1	68	P815R (CCC→CGC)	valS	valine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (33/31);  new base C (2/2);  total (35/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

GGTAGCGGACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCATGATGGTCTTGCCGTCGATGCC  >  NC_002947/1115240‑1115533                                                                                                                                                     |                                                                                                                                                  ggTAGCGGACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCgg                                                                                                                                                   <  4:113143/149‑1 (MQ=255)       ggACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGa                                                                                                                                             >  7:115045/1‑149 (MQ=255)       ggACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGa                                                                                                                                             >  7:37498/1‑149 (MQ=255)        gACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGAGCTTGGCCAGCTTCTTCTGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCTGGAACAGCGGCAGCGGCTTGCCGGGGCCGAt                                                                                                                                            <  2:270753/149‑1 (MQ=255)         aCAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGATCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACATCGGCCCCGGCAAGccgctgccgctg                                                                                                                                           <  1:17700/149‑1 (MQ=255)           aGCGGCGCTTCGTCGGCATCACCCAGTACAGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGtt                                                                                                                                         >  4:210446/1‑149 (MQ=255)             cggcgCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACATCGGCCCCGGCAAGCCGCTGCCGCTGTTcc                                                                                                                                       <  4:225660/149‑2 (MQ=38)               gcgcTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCgggg                                                                                                                                                 >  4:195338/1‑137 (MQ=255)               gcgcTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCgggg                                                                                                                                                 <  3:195338/137‑1 (MQ=255)               gcgcTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGGCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCAtt                                                                                                                                     <  8:163844/149‑1 (MQ=255)                    tcgtcgGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGgc                                                                                                                                >  7:69324/1‑149 (MQ=255)                     cgtcgGCATCACCCAGTACAGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGgc                                                                                                                                <  3:210446/148‑1 (MQ=255)                      gtcgGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGgcgc                                                                                                                              <  8:69324/149‑1 (MQ=255)                       tcgGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGcgcg                                                                                                                             >  3:205029/1‑149 (MQ=255)                          gCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGc                                                                                                                                                <  8:174675/127‑1 (MQ=255)                             tCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACATCGGCCCCGGCAAGCCGCTGCCGCTGTTc                                                                                                                                        >  3:228995/1‑132 (MQ=38)                             tCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACATCGGCCCCGGCAAGCCGCTGCCGCTGTTc                                                                                                                                        <  4:228995/132‑1 (MQ=38)                             tCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTc                                                                                                                                   <  3:307835/137‑1 (MQ=255)                             tCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTc                                                                                                                                   >  4:307835/1‑137 (MQ=255)                                 ccAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTgcgc                                                                                                                   <  1:311261/149‑1 (MQ=255)                                   aGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTc                                                                                                                                        <  8:208284/126‑1 (MQ=255)                                   aGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTc                                                                                                                                        >  7:208284/1‑126 (MQ=255)                                     tACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGc                                                                                                               <  8:37498/149‑1 (MQ=255)                                        ggTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGa                                                                                                            >  7:214241/1‑149 (MQ=255)                                            aaCGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCa                                                                                                         >  5:242935/1‑148 (MQ=255)                                               gACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCaa                                                                                                     <  3:152751/149‑1 (MQ=255)                                                  tCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCG‑TTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAActct                                                                                                 >  7:55369/1‑149 (MQ=255)                                                    gACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCtt                                                                                                >  8:187144/1‑149 (MQ=255)                                                     aCCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGAc                                                                                                           <  3:17019/137‑1 (MQ=255)                                                     aCCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGAc                                                                                                           >  4:17019/1‑137 (MQ=255)                                                      ccTTTGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTc                                                                                              <  4:205029/149‑1 (MQ=255)                                                       cTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCa                                                                                             <  8:214241/149‑1 (MQ=255)                                                           gCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCa                                                                                         >  4:219394/1‑149 (MQ=255)                                                           gCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCa                                                                                         >  4:164010/1‑149 (MQ=255)                                                            ccAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCAc                                                                                        <  7:187144/149‑1 (MQ=255)                                                             cAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACt                                                                                       >  1:45662/1‑149 (MQ=255)                                                              aGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCCGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTc                                                                                      <  5:57533/149‑1 (MQ=255)                                                               gCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCAt                                                                                                        <  2:273326/130‑1 (MQ=255)                                                               gCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCAt                                                                                                        >  1:273326/1‑130 (MQ=255)                                                                cttcttCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAg                                                                                                                 <  7:232783/120‑1 (MQ=255)                                                                cttcttCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAg                                                                                                                 >  8:232783/1‑120 (MQ=255)                                                                    ttcAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCa                                                                                             >  1:48226/1‑136 (MQ=255)                                                                    ttcAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCa                                                                                             <  2:48226/136‑1 (MQ=255)                                                                        gcagcGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTc                                                                                              >  2:160856/1‑131 (MQ=255)                                                                          agcGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTc                                                                          <  5:33074/149‑1 (MQ=255)                                                                              cTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCgg                                                                        >  1:170307/1‑147 (MQ=255)                                                                              cTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCa                                                                      <  6:242935/149‑1 (MQ=255)                                                                                   gtttCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAgcggc                                                                 <  8:55369/148‑1 (MQ=255)                                                                                    tttCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCg                                                                         >  6:138783/1‑140 (MQ=255)                                                                                    tttCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCg                                                                         <  5:138783/140‑1 (MQ=255)                                                                                        cTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGAc                                                                                                           >  1:284768/1‑102 (MQ=255)                                                                                        cTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGAc                                                                                                           <  2:284768/102‑1 (MQ=255)                                                                                         tGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCaa                                                            >  5:203271/1‑148 (MQ=255)                                                                                           cAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAAt                                                           <  8:115045/147‑1 (MQ=255)                                                                                               cggcgCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGc                                                     <  3:164010/149‑1 (MQ=255)                                                                                               cggcgCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGc                                                     <  3:219394/149‑1 (MQ=255)                                                                                                      ggTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTg                                              >  2:243351/1‑149 (MQ=255)                                                                                                          gtcgGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGt                                                       >  8:65510/1‑136 (MQ=255)                                                                                                          gtcgGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGt                                                       <  7:65510/136‑1 (MQ=255)                                                                                                           tcgGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAAt                                                           <  8:86964/131‑1 (MQ=255)                                                                                                           tcgGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAAt                                                           >  7:86964/1‑131 (MQ=255)                                                                                                                    gcgttCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATgg                                <  2:170307/149‑1 (MQ=255)                                                                                                                          gaaatgAACATCGGCCCCGGCAAGCCGCTGCCGCTGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTgcag                          >  5:361737/6‑149 (MQ=38)                                                                                                                           tcAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTgcag                          >  2:299957/1‑148 (MQ=255)                                                                                                                              ggAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCat                       >  1:199262/1‑148 (MQ=255)                                                                                                                                    gcggcagcggcTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCATGATGGTc                <  6:203271/149‑1 (MQ=255)                                                                                                                                     cggcagcggcTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCATGATGGTCt               >  3:107982/1‑149 (MQ=255)                                                                                                                                       gcagcggcTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCATGATGGTCTTg             <  1:299957/149‑1 (MQ=255)                                                                                                                                          gcggcTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGGAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCATGATGGTCTTGCCg          >  4:133913/1‑149 (MQ=255)                                                                                                                                             gcTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCg                                                                                     >  2:72931/1‑71 (MQ=255)                                                                                                                                               ttGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCATGATGGTCTTGCCGTCGAt     >  3:263463/1‑149 (MQ=255)                                                                                                                                                  ccGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCATGATGGTCTTGCCGTCGATGcc  >  6:328792/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                  GGTAGCGGACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCATGATGGTCTTGCCGTCGATGCC  >  NC_002947/1115240‑1115533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑