Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,115,394	A→C	6.0%	I813S (ATC→AGC)	valS ←	valine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,115,394	0	A	C	6.0%	207.5 / 3.4	66	I813S (ATC→AGC)	valS	valine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (31/31);  new base C (2/2);  total (33/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCATGATGGTCTTGCCGTCGATGCCGGCCA  >  NC_002947/1115246‑1115538                                                                                                                                                     |                                                                                                                                                 ggACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGa                                                                                                                                                  >  7:115045/1‑149 (MQ=255) ggACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGa                                                                                                                                                  >  7:37498/1‑149 (MQ=255)  gACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGAGCTTGGCCAGCTTCTTCTGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCTGGAACAGCGGCAGCGGCTTGCCGGGGCCGAt                                                                                                                                                 <  2:270753/149‑1 (MQ=255)   aCAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGATCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACATCGGCCCCGGCAAGccgctgccgctg                                                                                                                                                <  1:17700/149‑1 (MQ=255)     aGCGGCGCTTCGTCGGCATCACCCAGTACAGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGtt                                                                                                                                              >  4:210446/1‑149 (MQ=255)       cggcgCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACATCGGCCCCGGCAAGCCGCTGCCGCTGTTcc                                                                                                                                            <  4:225660/149‑2 (MQ=38)         gcgcTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGGCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCAtt                                                                                                                                          <  8:163844/149‑1 (MQ=255)              tcgtcgGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGgc                                                                                                                                     >  7:69324/1‑149 (MQ=255)               cgtcgGCATCACCCAGTACAGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGgc                                                                                                                                     <  3:210446/148‑1 (MQ=255)                gtcgGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGgcgc                                                                                                                                   <  8:69324/149‑1 (MQ=255)                 tcgGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGcgcg                                                                                                                                  >  3:205029/1‑149 (MQ=255)                       tCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACATCGGCCCCGGCAAGCCGCTGCCGCTGTTc                                                                                                                                             >  3:228995/1‑132 (MQ=38)                       tCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACATCGGCCCCGGCAAGCCGCTGCCGCTGTTc                                                                                                                                             <  4:228995/132‑1 (MQ=38)                       tCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTc                                                                                                                                        <  3:307835/137‑1 (MQ=255)                       tCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTc                                                                                                                                        >  4:307835/1‑137 (MQ=255)                           ccAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTgcgc                                                                                                                        <  1:311261/149‑1 (MQ=255)                             aGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTc                                                                                                                                             <  8:208284/126‑1 (MQ=255)                             aGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTc                                                                                                                                             >  7:208284/1‑126 (MQ=255)                               tACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGc                                                                                                                    <  8:37498/149‑1 (MQ=255)                                  ggTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGa                                                                                                                 >  7:214241/1‑149 (MQ=255)                                      aaCGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCa                                                                                                              >  5:242935/1‑148 (MQ=255)                                         gACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCaa                                                                                                          <  3:152751/149‑1 (MQ=255)                                            tCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCG‑TTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAActct                                                                                                      >  7:55369/1‑149 (MQ=255)                                              gACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCtt                                                                                                     >  8:187144/1‑149 (MQ=255)                                               aCCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGAc                                                                                                                <  3:17019/137‑1 (MQ=255)                                               aCCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGAc                                                                                                                >  4:17019/1‑137 (MQ=255)                                                ccTTTGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTc                                                                                                   <  4:205029/149‑1 (MQ=255)                                                 cTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCa                                                                                                  <  8:214241/149‑1 (MQ=255)                                                     gCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCa                                                                                              >  4:219394/1‑149 (MQ=255)                                                     gCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCa                                                                                              >  4:164010/1‑149 (MQ=255)                                                      ccAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCAc                                                                                             <  7:187144/149‑1 (MQ=255)                                                       cAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACt                                                                                            >  1:45662/1‑149 (MQ=255)                                                        aGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCCGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTc                                                                                           <  5:57533/149‑1 (MQ=255)                                                         gCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCAt                                                                                                             <  2:273326/130‑1 (MQ=255)                                                         gCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCAt                                                                                                             >  1:273326/1‑130 (MQ=255)                                                          cttcttCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAg                                                                                                                      <  7:232783/120‑1 (MQ=255)                                                          cttcttCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAg                                                                                                                      >  8:232783/1‑120 (MQ=255)                                                              ttcAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCa                                                                                                  >  1:48226/1‑136 (MQ=255)                                                              ttcAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCa                                                                                                  <  2:48226/136‑1 (MQ=255)                                                                  gcagcGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTc                                                                                                   >  2:160856/1‑131 (MQ=255)                                                                    agcGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTc                                                                               <  5:33074/149‑1 (MQ=255)                                                                        cTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCgg                                                                             >  1:170307/1‑147 (MQ=255)                                                                        cTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCa                                                                           <  6:242935/149‑1 (MQ=255)                                                                             gtttCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAgcggc                                                                      <  8:55369/148‑1 (MQ=255)                                                                              tttCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCg                                                                              >  6:138783/1‑140 (MQ=255)                                                                              tttCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCg                                                                              <  5:138783/140‑1 (MQ=255)                                                                                  cTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGAc                                                                                                                >  1:284768/1‑102 (MQ=255)                                                                                  cTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGAc                                                                                                                <  2:284768/102‑1 (MQ=255)                                                                                   tGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCaa                                                                 >  5:203271/1‑148 (MQ=255)                                                                                     cAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAAt                                                                <  8:115045/147‑1 (MQ=255)                                                                                         cggcgCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGc                                                          <  3:219394/149‑1 (MQ=255)                                                                                         cggcgCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGc                                                          <  3:164010/149‑1 (MQ=255)                                                                                                ggTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTg                                                   >  2:243351/1‑149 (MQ=255)                                                                                                    gtcgGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGt                                                            >  8:65510/1‑136 (MQ=255)                                                                                                    gtcgGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGt                                                            <  7:65510/136‑1 (MQ=255)                                                                                                     tcgGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAAt                                                                >  7:86964/1‑131 (MQ=255)                                                                                                     tcgGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAAt                                                                <  8:86964/131‑1 (MQ=255)                                                                                                              gcgttCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATgg                                     <  2:170307/149‑1 (MQ=255)                                                                                                                    gaaatgAACATCGGCCCCGGCAAGCCGCTGCCGCTGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTgcag                               >  5:361737/6‑149 (MQ=38)                                                                                                                     tcAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTgcag                               >  2:299957/1‑148 (MQ=255)                                                                                                                        ggAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCat                            >  1:199262/1‑148 (MQ=255)                                                                                                                              gcggcagcggcTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCATGATGGTc                     <  6:203271/149‑1 (MQ=255)                                                                                                                               cggcagcggcTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCATGATGGTCt                    >  3:107982/1‑149 (MQ=255)                                                                                                                                 gcagcggcTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCATGATGGTCTTg                  <  1:299957/149‑1 (MQ=255)                                                                                                                                    gcggcTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGGAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCATGATGGTCTTGCCg               >  4:133913/1‑149 (MQ=255)                                                                                                                                       gcTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCg                                                                                          >  2:72931/1‑71 (MQ=255)                                                                                                                                         ttGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCATGATGGTCTTGCCGTCGAt          >  3:263463/1‑149 (MQ=255)                                                                                                                                            ccGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCATGATGGTCTTGCCGTCGATGcc       >  6:328792/1‑149 (MQ=255)                                                                                                                                                 gCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACAGGCCATGGCTTCAGCATGATGGTCTTGCCGTCGATGCCGGCCa  <  1:243351/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                 GGACAGCGGCGCTTCGTCGGCATCACCCAGTACGGTGAACGACTCGACCTTGGCCAGCTTCTTCAGCAGCGCTTCGTTTTCCTGCAGGCGGCGCTGGTCGTCGGCGTTGGCGTTCTTCAGGAACAGCGGCAGCGGCTTGCCGGGGCCGATGTTCATTTCGGCGCGGATATTGCGCAGGCCGACCATCAACTCTTTCAGCCACTCGATGTCGCCTTCGGCAGCGGCGTCAATACGTGCTTCGTTGGCCACTGGCCATGGCTGCAGCATGATGGTCTTGCCGTCGATGCCGGCCA  >  NC_002947/1115246‑1115538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑