Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,145,440 G→A 16.0% intergenic (‑107/+34) hemO ← / ← PP_1006 heme oxygenase/heme receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,145,4400GA16.0% 117.1 / 11.8 50intergenic (‑107/+34)hemO/PP_1006heme oxygenase/heme receptor
Reads supporting (aligned to +/- strand):  ref base G (20/22);  new base A (4/4);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

GTTCAGGCGCTGGGAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACG‑CCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAGCTGCGC  >  NC_002947/1145295‑1145582
                                                                                                                                                 |                                                                                                                                               
gTTCAGGCGCTGGGAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGc                                                                                                                                              >  6:121169/1‑149 (MQ=255)
gTTCAGGCGCTGGGAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGc                                                                                                                                              >  6:52946/1‑149 (MQ=255)
gTTCAGGCGCTGGGAGCGCAGGGCTGGGCGGGCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGc                                                                                                                                              <  8:247867/149‑1 (MQ=255)
      gcgcTGGGAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCa                                                                                                                                         >  6:344714/1‑148 (MQ=255)
      gcgcTGGGAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCACTACCGGCCTCTTCGCGGGCGTGCCCGCaa                                                                                                                                        <  3:62507/149‑1 (MQ=255)
         cTGGGAGCGCAGGGCTGGGCGGTCGGTCAGGGGGGAGCGCTTGGAAAGAGCGCGTCTAGATACAACACCAAACAGCGGGAGCGAGAGAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAaga                                                                                                                                     <  7:146089/149‑1 (MQ=255)
            ggAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTTGCGGGCACGCCCGCGAagag                                                                                                                                    <  4:266598/147‑1 (MQ=255)
            ggAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGc                                                                                                                                  <  5:121169/149‑1 (MQ=255)
            ggAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGc                                                                                                                                  >  4:140625/1‑149 (MQ=255)
              aGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTTGCGGGCACGCCCGCGAAGAGGCCg                                                                                                                                <  5:269130/149‑1 (MQ=255)
               gcgcAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCgg                                                                                                                               >  3:35728/1‑149 (MQ=255)
               gcgcAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCgg                                                                                                                               >  5:231315/1‑149 (MQ=255)
                cgcAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCgg                                                                                                                               >  6:349792/1‑148 (MQ=255)
                 gcAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTa                                                                                                                             <  7:285255/149‑1 (MQ=255)
                                 ggtcATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCg                                                                                                                                <  4:285439/130‑1 (MQ=255)
                                 ggtcATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACG‑CCTCa                                                                                                            <  5:52946/149‑1 (MQ=255)
                                 ggtcATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGACCGCAAAGAGGCCg                                                                                                                                >  3:285439/1‑130 (MQ=255)
                                        ggggAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACG‑CCTCAGATATcc                                                                                                     >  2:125843/1‑149 (MQ=255)
                                                   ggAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCTGGTACAGACGTAACG‑CCTCAGATATCCCAAACCACAt                                                                                           <  1:274018/148‑1 (MQ=255)
                                                   ggAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACG‑CCTCAGATATCCCAAACCACAt                                                                                           <  6:231315/148‑1 (MQ=255)
                                                   ggAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACG‑CCTCAGATATCCCAAACCACAt                                                                                           <  5:344714/148‑1 (MQ=255)
                                                   ggAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACG‑CCTCAGATATCCCAAACCACAt                                                                                           <  4:35728/148‑1 (MQ=255)
                                                       agagGGCGTCTATATACAAGACGAAACAGCCGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACG‑CCTCAGATATCCCAAACCACATTGATa                                                                                      <  3:320673/149‑1 (MQ=255)
                                                             cGTCTAGATACAAGACGAAACAGCGGCAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACG‑CCTCAGATATCCCAAACCACATTGATACCGAAg                                                                                <  8:174138/149‑1 (MQ=255)
                                                                   gATACAAGACGAAACAGCGCGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACG‑CCTCAGATATCCCAAACCACATTGATACCGAAGTTgcgc                                                                          >  7:242185/1‑149 (MQ=255)
                                                                          gACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACG‑CCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCa                                                                   >  3:318271/1‑149 (MQ=255)
                                                                          caCGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCCCGGGCGTGCCCGCAAACAGGCCCGTACAGGCGAAACG‑CATCAGATATCACCAACCACAGTGATACCGAAGTTGCGCCCCGGCa                                                                   >  4:153487/2‑149 (MQ=255)
                                                                            cGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACG‑CCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATg                                                                 <  3:140625/149‑1 (MQ=255)
                                                                              aaaCAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACG‑CCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGt                                                               >  6:364772/1‑149 (MQ=255)
                                                                                    cGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACG‑CCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCg                                                         >  6:17857/1‑149 (MQ=255)
                                                                                           gagaCAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACG‑CCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATg                                                  >  3:92112/1‑149 (MQ=255)
                                                                                            agaCAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACG‑CCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGt                                                 <  5:17857/149‑1 (MQ=255)
                                                                                               cAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACG‑CCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTtgg                                              <  1:125843/149‑1 (MQ=255)
                                                                                                  tAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACG‑CCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGa                                                    <  4:114134/140‑1 (MQ=255)
                                                                                                  tAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACG‑CCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGa                                                    >  3:114134/1‑140 (MQ=255)
                                                                                                       aaaGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACG‑CCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGctggctgg                                      <  4:92112/149‑1 (MQ=255)
                                                                                                             ggTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGGGCCCGCAAAGAGGCCGGTACAGACGTAACG‑CCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTcaca                                <  5:349792/149‑1 (MQ=255)
                                                                                                                      gTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACG‑CCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCa                       >  2:324106/1‑149 (MQ=255)
                                                                                                                          tctgTACCGGCCTCTTTGCGGGCACGCCCGCGAAGAGGCCGGTACAGACGTAACG‑CCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGt                                                       <  4:115363/110‑1 (MQ=37)
                                                                                                                          ccAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACG‑CCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTc                                                              >  1:322233/1‑106 (MQ=255)
                                                                                                                          ccAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACG‑CCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTc                                                              <  2:322233/106‑1 (MQ=255)
                                                                                                                                 cGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACG‑CCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCcgtc            <  1:324106/149‑1 (MQ=255)
                                                                                                                                  ggCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACG‑CCTCAGATATCCCAAACAACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGAGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCcgtct           >  6:359913/1‑148 (MQ=255)
                                                                                                                                   gCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACG‑CCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGctggctgg                                      <  6:339024/121‑1 (MQ=255)
                                                                                                                                   gCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACG‑CCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGctggctgg                                      >  5:339024/1‑121 (MQ=255)
                                                                                                                                     ctctTTGCGGGCACGCCCGCGAAGAGGCCGGTACAGACGTAACG‑CCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAg        >  1:119109/1‑149 (MQ=255)
                                                                                                                                      tctTTGCGGGCACGCCCGCGAAGAGGCCGGTACAGACGTAACGCCCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAg        >  1:68573/1‑149 (MQ=255)
                                                                                                                                      tctTTGCGGGCACGCCCGCGAAGAGGCCGGTACAGACGTAACG‑CCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAGc       >  7:47799/1‑149 (MQ=255)
                                                                                                                                       ctTTGCGGGCACGCCCGCGAAGAGGCCGGTACAGACGTAACG‑CCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAGCt      >  4:329220/1‑149 (MQ=255)
                                                                                                                                            cgGGCACGCCCGCGAAGAGGCCGGTACAGACGTAACG‑CCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAGCTgcgc  <  3:329220/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GTTCAGGCGCTGGGAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGCCTCTTCGCGGGCGTGCCCGCAAAGAGGCCGGTACAGACGTAACG‑CCTCAGATATCCCAAACCACATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCGTCGTAGCTGCGC  >  NC_002947/1145295‑1145582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑