| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 35,009 | A→G | 11.5% | intergenic (+27/+31) | czrSA → / ← PP_0031 | metal‑sensor histidine kinase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 35,009 | 0 | A | G | 11.5% | 130.1 / 8.3 | 52 | intergenic (+27/+31) | czrSA/PP_0031 | metal‑sensor histidine kinase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (25/21); new base G (2/4); total (27/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01 | |||||||||||
GATCATGAGTCTGCACCAGGGATCGGCGAGCGTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGA‑CACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGTTTTTCCACAGGTGTGAGAAGTACTGAGCATTCACTTTC > NC_002947/34865‑35157 | gATCATGAGTCTGCACCAGGGATCGGCGAGCGTGGAGGTGACAGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGA‑CACGATTAGGCCCGTCAATc > 1:67469/1‑148 (MQ=255) gTCTTCACCAGGGATCGGCGAGCGTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGA‑CACGATTAGGCCCGTCAAt < 3:207709/139‑1 (MQ=255) gTCTGCACCAGGGATCGGCGAGCGTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGA‑CACGATTAGGCCCGTCAAt > 4:207709/1‑139 (MQ=255) tCTGCACCAGGGATCGGCGAGCGTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGA‑CACGATTAGGCCCGTCGATTGACGGGCCt > 2:176470/1‑148 (MQ=255) cACCAGGGATCGGCGAGCGTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGA‑CACGATTAGGCCCGTCGATTGACGGGCCtaa < 7:275728/146‑3 (MQ=255) cACCAGGGATCGGCGAGCGTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGA‑CACGATTAGGCCCGTCGATTGACGGGCCtaa < 4:153778/146‑3 (MQ=255) cACCAGGGATCGGCGAGCGTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGA‑CACGATTAGGCCCGTCAATCGACGGGCCTtttgt > 1:169235/1‑149 (MQ=255) cACCAGGGATCGGCGAGCGTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGA‑CACGATTAGGCCCGTCAATCGACGGGCCTtttgt < 2:67469/149‑1 (MQ=255) aCCAGGGATCGGCGAGCGTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGA‑CACGATTAGGCCCGTCGATTGACGGGCCtaatcgt < 1:176470/149‑7 (MQ=255) ggATCGGCGAGCGTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGA‑CACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCt < 2:34840/149‑1 (MQ=255) tCGGCGAGCGTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGA‑CACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCaa < 7:243732/149‑1 (MQ=255) ggCGAGCGTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACCACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCaa < 1:242394/148‑1 (MQ=255) ggCGAGCGTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGA‑CACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAAt < 2:169235/148‑1 (MQ=255) gCGTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGA‑CACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCAt > 2:104101/1‑147 (MQ=255) gTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGA‑CACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGt > 4:78880/1‑149 (MQ=255) gTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGA‑CACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGaa < 3:78880/149‑1 (MQ=255) gACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGA‑CACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAg > 7:149441/1‑148 (MQ=255) ccGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGA‑CACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGAc < 8:149441/149‑1 (MQ=255) cGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGA‑CACGATTAGGCCCGTCGATTGACgg > 4:55583/1‑111 (MQ=255) cGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGA‑CACGATTAGGCCCGTCGATTGACgg < 3:55583/111‑1 (MQ=255) cGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGA‑CACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGAc > 8:316146/1‑148 (MQ=255) ttCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGA‑CACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCa > 3:156661/1‑149 (MQ=255) aCCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGA‑CACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATgg < 4:156661/149‑1 (MQ=255) ttCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGA‑CACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGAcc > 8:311584/1‑149 (MQ=255) tCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGA‑CACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAAt > 8:2878/1‑148 (MQ=255) tttCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGA‑CACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCAcc > 3:29203/1‑148 (MQ=255) tttCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGA‑CACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCAcc > 4:347288/1‑148 (MQ=255) tttCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGA‑CACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCAcc > 5:139540/1‑148 (MQ=255) aaGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGA‑CACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTtaa > 6:302155/1‑148 (MQ=255) aGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGA‑CACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTtaata < 7:311584/149‑1 (MQ=255) aGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGA‑CACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTtaata < 6:139540/149‑1 (MQ=255) cAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGA‑CACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCTTCAGTGCCGAAGGACCACAg < 6:268725/110‑1 (MQ=255) cAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGA‑CACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCGTCAGTGCCGAAGGACCACAg > 5:268725/1‑110 (MQ=255) cAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGA‑CACGATTAGGCCCGTCAATCGAAGGGCCTTTTGTTTGCCTCAATGAATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAAtc > 1:121270/1‑148 (MQ=255) aGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGA‑CACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAAtctt < 5:302155/149‑1 (MQ=255) cGGCAATGACCGCCGCTTAAATTGCGCAGA‑CACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAg > 3:328994/1‑148 (MQ=255) tGACCGCCGCTTAAATTGCGCAGA‑CACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGc < 7:316146/149‑1 (MQ=255) aCCGCCGCTTAAATTGCGCAGA‑CACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGt > 2:274702/1‑149 (MQ=255) ccgccgCTTAAATTGCGCAGA‑CACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGtt > 5:74344/1‑149 (MQ=255) cgcTTAAATTGCGCAGA‑CACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGTTTTTc < 7:2878/149‑1 (MQ=255) gcTTAAATTGCGCAGA‑CACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTtaa < 2:342980/116‑1 (MQ=255) ttAAATTGCGCAGA‑CACTATTAGGCCCGTCAATTGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACcaca < 4:207451/80‑1 (MQ=255) ttAAATTGCGCAGA‑CACAATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACcaca > 3:207451/1‑80 (MQ=255) aaaTTGCGCAGA‑CACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGTTTTTCcaca < 3:347288/148‑1 (MQ=255) aTTGCGCAGA‑CACGATTATTCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATTCATCAGTGCAGAATGACCACAGGGTCCATTGGCTACAGCAGAATCACCGACTTAATAATCTTCTGTTCTTCATGATTGGCGTTTTTCCACAGgt < 2:121270/149‑1 (MQ=255) a‑cacGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGTTTTTCCACAGGTGTGAGAAg > 7:25894/1‑148 (MQ=255) cacGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGTTTTTCCACAGGTGTGAGAAGTa < 4:29203/149‑1 (MQ=255) cccGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGTTTTTCCACAGGTGTGAGAAGTACTGAGCAtt > 5:39747/1‑148 (MQ=255) ccGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGTTTTTCCACAGGTGTGAGAAGTACTGAGCATTc > 1:121089/1‑148 (MQ=255) ccGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGTTTTTCCACAGGTGTGAGAAGTACTGAGCATTCa > 7:163090/1‑149 (MQ=255) gTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGTTTTTCCACAGGTGTGAGAAGTACTGAGCATTCACt > 3:318598/1‑149 (MQ=255) tCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGTTTTTCCACAGGTGTGAGAAGTACTGAGCATTCACtt < 2:121089/149‑1 (MQ=255) aaTCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGTTTTTCCACAGGTGTGAGAAGTACTGAGCATTCACTTtc < 8:25894/149‑1 (MQ=255) aaTCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGTTTTTCCACAGGTGTGAGAAGTACTGAGCATTCACTTtc > 6:138946/1‑149 (MQ=255) | GATCATGAGTCTGCACCAGGGATCGGCGAGCGTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGA‑CACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGTTTTTCCACAGGTGTGAGAAGTACTGAGCATTCACTTTC > NC_002947/34865‑35157 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |