Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,232,572	T→C	6.8%	K47K (AAA→AAG)	glpD ←	aerobic glycerol‑3‑phosphate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,232,572	0	T	C	6.8%	165.3 / 2.3	59	K47K (AAA→AAG)	glpD	aerobic glycerol‑3‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (27/28);  new base C (2/2);  total (29/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.54e-01

GTTTGACGATGTGGGGCGCCTTCGCCAGCAGCACTTCGCGTTCAGCCAGGGCCTCGCGTACAAGACGAAATTCGTAGTGCTCCAGGTAGCGCAGGCCACCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAGCG‑GCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGC  >  NC_002947/1232424‑1232715                                                                                                                                                     |                                                                                                                                                     gTTTGACGATGTGGGGCGCCTTCGCCAGCAGCACTTCGCGTTCAGCCAGGGCCTCGCGTACAAGACGAAATTCGTAGTGCTCCAGGTAGCGCAGGCCACCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCt                                                                                                                                                      >  7:7787/1‑149 (MQ=255) gTTTGACGATGTGGGGCGCCTTCGCCAGCAGCACTTCGCGTTCAGCCAGGGCCTCGCGTACAAGACGAAATTCGTAGTGCTCCAGGTAGCGCAGGCCACCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCt                                                                                                                                                      >  5:146752/1‑149 (MQ=255) gTTTGACGATGTGGGGCGCCTTCGCCAGCAGCACTTCGCGTTCAGCCAGGGCCTCGCGTACAAGACGAAATTCGTAGTGCTCCAGGTAGCGCAGGCCACCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCt                                                                                                                                                      >  7:2596/1‑149 (MQ=255)  tttGACGATGTGGGGCGCCTTCGCCAGCAGCACTTCGCGTTCAGCCAGGGCCTCGCGTACAAGACGAAATTCGTAGTGCTCCAGGTAGCGCAGGCCACCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCtt                                                                                                                                                     >  5:48947/1‑149 (MQ=255)           gtgGGGCGCCTTCGCCAGCAGCACTTCGCGTTCAGCCAGGGCCTCGCGTACAAGACGAAATTCGTAGTGCTCCAGGTAGCGCAGGCCACCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCa                                                                                                                                              >  1:67104/1‑147 (MQ=255)             ggggCGCCTTCGCCAGCAGCACTTCGCGTTCAGCCAGGGCCTCGCGTACAAGACGAAATTCGTAGTGCTCCAGGTAGCGCAGGCCACCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAg                                                                                                                                           >  8:326288/1‑148 (MQ=255)               ggCGCCTTCGCCAGCAGCACTTCGCGTTCAGCCAGGGCCTCGCGTACAAGACGAAATTCGTAGTGCTCCAGGTAGCGCAGGCCACCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAg‑‑‑‑g                                                                                                                                      >  2:56619/1‑147 (MQ=255)               ggCGCCTTCGCCAGCAGCACTTCGCGTTCAGCCAGGGCCTCGCGTACAAGACGAAATTCGTAGTGCTCCAGGTAGCGCAGGCCACCGAGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑Gaa                                                                                                                                    <  7:197012/149‑1 (MQ=255)                     ttCGCCAGCAGCACTTCGCGTTCAGCCAGGGCCTCGCGTACAAGACGAAATTCGTAGTGCTCCAGGTAGCGCAGGCCACCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCTTCCTTTGCGAAAAA‑‑‑‑GACGACCtg                                                                                                                              >  6:261829/1‑148 (MQ=255)                       cGCCAGCAGCACTTCGCGTTCATCCAGGGCCTCGCGTACATGACGAAATTCTTAGTGCTCCAGGTAGCGCAGGCCACCGTGAATAAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCtt                                                                                                                              <  5:170606/147‑1 (MQ=255)                       cGCCAGCAGCACTTCGCGTTCAGCCAGGGCCTCGCGTACAAGACGAAATTCGTAGTGCTCCAGGTAGCGCAGGCCACCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCTTCCTTTGCGAAAAA‑‑‑‑GACGACCtgg                                                                                                                             <  5:261829/148‑3 (MQ=255)                       cGCCAGCAGCACTTCGCGTTCAGCCAGGGCCTCGCGTACAAGACGAAATTCGTAGTGCTCCAGGTAGCGCAGGCCACCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCtt                                                                                                                              <  1:228429/147‑1 (MQ=255)                             gagcaCTTCGCGTTCAGCCAGGGCCTCGCGTACAAGACGAAATTCGTAGTGCTCCAGGTAGCGCAGGCCACCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCcgc                                                                                                                      <  2:67104/148‑1 (MQ=255)                                caCTTCGCGTTCAGCCAGGGCCTCGCGTACAAGACGAAACTCGTAGTGCTCCAGGTAGCGCAGGCCACCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGAc                                                                                                                   >  2:97804/1‑149 (MQ=255)                                  cTTCGCGTTCAGCCAGGGCCTCGCGTACAAGACGAAATTCGTAGTGCTCCAGGTAGCGCAGGCCACCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAg                                                                                                                                   <  3:113919/131‑1 (MQ=255)                                  cTTCGCGTTCAGCCAGGGCCTCGCGTACAAGACGAAATTCGTAGTGCTCCAGGTAGCGCAGGCCACCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAg                                                                                                                                   >  4:113919/1‑131 (MQ=255)                                       cgTTCAGCCAGGGCCTCGCGTACAAGACGAAATTCGTAGTGCTCCAGGTAGCGCAGGCCACCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAgcg‑gc                                                                                                           <  7:326288/149‑1 (MQ=255)                                        gTTCAGCCAGGGCCTCGCGTACAAGACGAAATTCGTAGTGCTCCAGGTAGCGCAGGCCACCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGAc                                                                                                                                 <  4:51160/127‑1 (MQ=255)                                        gTTCAGCCAGGGCCTCGCGTACAAGACGAAATTCGTAGTGCTCCAGGTAGCGCAGGCCACCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGAc                                                                                                                                 >  3:51160/1‑127 (MQ=255)                                          tCAGCCAGGGCCTCGCGTACAAGACGAAATTCGTAGTGCTCCAGGTAGCGCAGGCCACCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTc                                                                                                                             >  4:337200/1‑129 (MQ=255)                                          tCAGCCAGGGCCTCGCGTACAAGACGAAATTCGTAGTGCTCCAGGTAGCGCAGGCCACCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTc                                                                                                                             <  3:337200/129‑1 (MQ=255)                                           cAGCCAGGGCCTCGCGTACAAGACGAAATTCGTAGTGCTCCAGGTAGCGCAGGCCACCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAGCG‑GCGTc                                                                                                        <  8:7787/148‑1 (MQ=255)                                           cAGCCAGGGCCTCGCGTACAAGACGAAATTCGTAGTGCTCCAGGTAGCGCAGGCCACCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAGCG‑GCGTc                                                                                                        <  8:2596/148‑1 (MQ=255)                                              ccAGGGCCTCGCGTACAAGACGAAATTCGTAGTGCTCCAGGTAGCGCAGGCCACCTTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAGCG‑GCGTCGGCa                                                                                                    >  5:324872/1‑149 (MQ=255)                                              ccAGGGCCTCGCGTACAAGACGAAATTCGTAGTGCTCCAGGTAGCGCAGGCCACCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAGCG‑GCGTCgg                                                                                                      >  7:31021/1‑147 (MQ=255)                                                 gggCCTCGCGTACAAGACGAAATTCGTAGTGCTCCAGGTAGCGCAGGCCACCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAGCG‑GCGTCGGCAGc                                                                                                  >  8:51902/1‑148 (MQ=255)                                                      tCGCGTACAAGACGAAATTCGTAGTGCTCCAGGTAGCGCAGGCCACCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAGCG‑GCGTCGGCAGCGATGcc                                                                                            >  2:26655/1‑149 (MQ=255)                                                        gcgTACAAGACGAAATTCGTAGTGCTCCAGGTAGCGCAGGCCACCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAGCG‑GCGTCGGCAGCGATGcc                                                                                            <  6:48947/147‑1 (MQ=255)                                                         cgTACAAGACGAAACTCGTAGTGCTCCAGGTAGCGCAGGCCACCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAGCG‑GCGTCGGCAGCGATGCCCAc                                                                                         <  1:97804/149‑1 (MQ=255)                                                              aaGACGAAATTCGTAGTGCTCCAGGTAGCGCAGGCCACCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAGCG‑GCGTCGGCAGCGATGCCCACGCCAt                                                                                    <  8:31021/149‑1 (MQ=255)                                                              aaGACGAAATTCGTAGTGCTCCAGGTAGCGCAGGCCACCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAGCG‑GCGTCGGCAGCGATGCCCACGCCAt                                                                                    >  2:165210/1‑149 (MQ=255)                                                                gACGAAATTCGTAGTGCTCCAGGTAGCGCAGGCCACCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAGCG‑GCGTGGGCATCGCTGCCGACGccgctg                                                                                  <  1:56619/149‑5 (MQ=255)                                                                gACGAAATTCGTAGTGCTCCAGGTAGCGCAGGCCACCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAGCG‑GCGTCGGCAg                                                                                                   >  8:168659/1‑132 (MQ=255)                                                                gACGAAATTCGTAGTGCTCCAGGTAGCGCAGGCCACCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAGCG‑GCGTCGGCAg                                                                                                   <  7:168659/132‑1 (MQ=255)                                                                 aCGAAATTCGTAGTGCTCCAGGTAGCGCAGGCCACCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCaa                                                                                                                           <  5:126705/108‑1 (MQ=255)                                                                 aCGAAATTCGTAGTGCTCCAGGTAGCGCAGGCCACCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCaa                                                                                                                           >  6:126705/1‑108 (MQ=255)                                                                   gAAATTCGTAGTGCTCCAGGTAGCGCAGGCCACCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAGCGCGCGTCGGCAGCGATGCCCACGCCATTGAt                                                                                <  1:26655/149‑1 (MQ=255)                                                                                      gTAGCGCAGGCCACCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTACGCAAAG‑‑‑‑GAAGCCCTTCAAGCCGCGACCACCG‑GCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGCTTACGGc                                                              >  5:217658/1‑147 (MQ=255)                                                                                         gcgcAGGCCACCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAGCG‑GCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGt                                                         >  3:172553/1‑149 (MQ=255)                                                                                           gcAGGCCACCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAGAGCAGGAGACCTTCAAGCCGCGACCAGCG‑GCGTCCGCAGCGAAGCCCCCGCCAATGATGCCACCCCCGAATACCGCAAg                                                           >  1:108611/1‑149 (MQ=255)                                                                                                 cACCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAGCG‑GCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAg                                                 >  6:104584/1‑149 (MQ=255)                                                                                                 cACCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAGCG‑GCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAg                                                 >  1:149354/1‑149 (MQ=255)                                                                                                  aCCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCCTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAGCG‑GCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGt                                                >  6:74918/1‑149 (MQ=255)                                                                                                       gactCAGTTTGCGGCGGGCTGACGACGGGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAGCG‑GCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACg                                                                <  8:143209/125‑1 (MQ=255)                                                                                                       gAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAGCG‑GCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACg                                                                >  7:143209/1‑128 (MQ=255)                                                                                                          tCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAGCG‑GCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGtt                                        >  3:32426/1‑149 (MQ=255)                                                                                                           cAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAGCG‑GCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGtt                                        <  1:120983/148‑1 (MQ=255)                                                                                                           cAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAGCG‑GCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGtt                                        <  4:32426/148‑1 (MQ=255)                                                                                                            aGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAGCG‑GCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGtt                                        >  2:120983/1‑147 (MQ=255)                                                                                                                     ctggctgACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAGCG‑GCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTgg                               <  2:149354/147‑1 (MQ=255)                                                                                                                     ctggctgACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAGCG‑GCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGa                             <  2:42365/149‑1 (MQ=255)                                                                                                                      tgtgtgACGACGTGTGTTGGGCGAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACGAGCG‑GCGTCGGCAGCGATGCCCACGCCATTTATGCCACCGCCTATTAGGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGAc                            <  6:217658/145‑1 (MQ=255)                                                                                                                        gctgACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAGCG‑GCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCCGTTGGCTGTTGGGGGCTGGGAc                            <  7:51902/147‑1 (MQ=255)                                                                                                                              gacgTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAGCG‑GCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATa                                                     <  7:157996/116‑1 (MQ=255)                                                                                                                              gacgTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAGCG‑GCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATa                                                     >  8:157996/1‑116 (MQ=255)                                                                                                                               acgTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAGCG‑GCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAActggc                   >  5:327859/1‑149 (MQ=255)                                                                                                                                cgTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAGCG‑GCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAgg                                                          >  2:190869/1‑109 (MQ=255)                                                                                                                                cgTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAGCG‑GCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAgg                                                          <  1:190869/109‑1 (MQ=255)                                                                                                                                cgTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAGCG‑GCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAActggct                  <  2:108611/149‑1 (MQ=255)                                                                                                                                  tgtgtTGGGCCAGGTCGTCCTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAGCG‑GCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAActggctgg                <  5:74918/149‑1 (MQ=255)                                                                                                                                        gggCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAGCG‑GCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTAATAGCAGTTGGATGTTGGGGGCTGGGACGAAACTTGCTGGGACACg          >  4:239999/1‑149 (MQ=255)                                                                                                                                           ccAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAGCG‑GCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACggg        >  8:138835/1‑148 (MQ=255)                                                                                                                                                tcgtcTTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAGCG‑GCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGc  <  4:172553/149‑1 (MQ=255)                                                                                                                                                     tttttCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAGCG‑GCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACga         >  6:80631/1‑136 (MQ=255)                                                                                                                                                     tttttCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAGCG‑GCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACga         <  5:80631/137‑2 (MQ=255)                                                                                                                                                     |                                                                                                                                                     GTTTGACGATGTGGGGCGCCTTCGCCAGCAGCACTTCGCGTTCAGCCAGGGCCTCGCGTACAAGACGAAATTCGTAGTGCTCCAGGTAGCGCAGGCCACCGTGAATCAGTTTGCTGCTGGCTGACGACGTGTGTTGGGCCAGGTCGTCTTTTTCGCAAAG‑‑‑‑GAAGACCTTCAAGCCGCGACCAGCG‑GCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGC  >  NC_002947/1232424‑1232715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑