Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,235,074 G→A 8.0% A71V (GCG→GTG)  glpK ← glycerol kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,235,0740GA 8.0% 143.5 / 2.2 50A71V (GCG→GTG) glpKglycerol kinase
Reads supporting (aligned to +/- strand):  ref base G (19/27);  new base A (2/2);  total (21/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01

GAGCTGAGCACAGATCTCGGTGCTGCGGCGGCACTGCCAGACAATGGCGTTGTACACCGGACGCCCGGTTTCCTTGTCCCACACCACCGTGGTTTCGCGTTGGTTGGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTCG‑ACCATGGTGGCGCTTTGTGTGGCGAAAATCTCCATCGGGTCATGCTCGACCCAGCCCGCCTGCGGGTAATGCTGTGCGAACTCGCGCTGGGAGGTACCTACAACATTGGCGTCACGGTCGAAAATGATCGC  >  NC_002947/1234929‑1235222
                                                                                                                                                 |                                                                                                                                                     
gagctgagcACAGATCTCGGTGCTGCGGCGGCACTGCCAGACAATGGCGTTGTACACCGGACGCCCGGTTTCCTTGTCCCACACCACCGTGGTTTCGCGTTGGTTGGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGc                                                                                                                                                      <  8:110294/147‑1 (MQ=255)
  gctgagcACAGATCTCGGTGCTGCGGCGGCACTGCCAGACAATGGCGTTGTACACCGGACGCCCGGTTTCCTTGTCCCACACCACCGTGGTTTCGCGTTGGTTGGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTgg                                                                                                                                                  <  3:116838/149‑1 (MQ=255)
        cacaGATCTCGGTGCTGCGGCGGCACTGCCAGACAATGGCGTTGTACACCGGACGCCCGGTTTCCTTGTCCCACACCACCGTGGTTTCGCGTTGGTTGGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAgg                                                                                                                                            <  8:114466/149‑1 (MQ=255)
         acaGATCTCGGTGCTGCGGCGGCACTGCCAGACAATGGCGTTGTACACCGGACGCCCGGTTTCCTTGTCCCACACCACCGTGGTTTCGCGTTGGTTGGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAgg                                                                                                                                            <  8:114464/148‑1 (MQ=255)
         acaGATCTCGGTGCTGCGGCGGCACTGCCAGACAATGGCGTTGTACACCGGACGCCCGGTTTCCTTGTCCCACACCACCGTGGTTTCGCGTTGGTTGGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCACCTGGGCAAggg                                                                                                                                           >  5:315183/1‑149 (MQ=255)
             aTCTCGGTGCTGCGGCGGCACTGCCAGACATTGGCGTTGTACACCGGACGCCCGGTTTCCTTGTCCCACACCACCGTGGTTTCGCGTTGGTTGGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTc                                                                                                                                       >  1:31087/1‑149 (MQ=255)
                tcGGTGCTGCGGCGGCACTGCCAGACAATGGCGTTGTACACCGGACGCCCGGTTTCCTTGTCCCACACCACCGTGGTTTCGCGTTGGTTGGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTCG‑Ac                                                                                                                                   >  2:58549/1‑149 (MQ=255)
                tcGGTGCTGCGGCGGCACTGCCAGACAATGGCGTTGTACACCGGACGCCCGGTTTCCTTGTCCCACACCACCGTGGTTTCGCGTTGGTTGGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTCG‑Ac                                                                                                                                   >  4:79708/1‑149 (MQ=255)
                  ggTGCTGCGGCGGCACTGCCAGACAATGGCGTTGTACACCGGACGCCCGGTTTCCTTGTCCCACACCACCGTGGTTTCGCGTTGGTTGGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTc                                                                                                                                       <  3:198492/144‑1 (MQ=255)
                   gTGCTGCGGCGGCACTGCCAGACAATGGCGTTGTACACCGGACGCCCGGTTTCCTTGTCCCACACCACCGTGGTTTCGCGTTGGTTGGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTCG‑ACCAt                                                                                                                                <  3:79708/149‑1 (MQ=255)
                           gcggcACTGCCAGACAATGGCGTTGTACACCGGACGCCCGGTTTCCTTGTCCCACACCACCGTGGTTTCGCGTTGGTTGGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTCG‑ACCATGGTGgcgc                                                                                                                        <  7:185187/149‑1 (MQ=255)
                                aCTGCCAGACAATGGCGTTGTACACCGGACGCCCGGTTTCCTTGTCCCACACCACCGTGGTTTCGCGTTGGTTGGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTCG‑ACCATGGTGGCGCTTtgt                                                                                                                   <  2:239705/149‑1 (MQ=255)
                                    ccAGACAATGGCGTTGTACACCGGACGCCCGGTTTCCTTGTCCCACACCACCGTGGTTTCGCGTTGGTTGGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTCG‑ACCATGGTGGCGCTTTGTGTgg                                                                                                               <  4:127906/149‑1 (MQ=255)
                                             ggCGTTGTACACCGGACGCCCGGTTTCCTTGTCCCACACCACCGTGGTTTCGCGTTGGTTGGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTCG‑ACCATGGTGGCGCTTTGTGTGGCGAAAAtct                                                                                                      >  4:324847/1‑149 (MQ=255)
                                                    tACACCGGACGCCCGGTTTCCTTGTCCCACACCACCGTGGTTTCGCGTTGGTTGGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTCG‑ACCATGGTGGCGCTTTGTGTGGCGAAAATCTCCATCgg                                                                                               >  2:120663/1‑149 (MQ=255)
                                                       acCGGACGCCCGGTTTCCTTGTCCCACACCACCGTGGTTTCGCGTTGGTTGGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTc                                                                                                                                       <  4:180136/107‑1 (MQ=255)
                                                       acCGGACGCCCGGTTTCCTTGTCCCACACCACCGTGGTTTCGCGTTGGTTGGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTc                                                                                                                                       >  3:180136/1‑107 (MQ=255)
                                                        ccGGACGCCCGGTTTCCTTGTCCCACACCACCGTGGTTTCGCGTTGGTTGGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTCG‑ACCATGGTGGCGCTTTGTGTGGCGAAAATCTCCATCGGGTCa                                                                                           <  3:21905/149‑1 (MQ=255)
                                                         cGGACGCCCGGTTTCCTTGTCCCACACCACCGTGGTTTCGCGTTGGTTGGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTCG‑ACCAtgg                                                                                                                              <  6:255354/113‑1 (MQ=255)
                                                         cGGACGCCCGGTTTCCTTGTCCCACACCACCGTGGTTTCGCGTTGGTTGGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTCG‑ACCAtgg                                                                                                                              >  5:255354/1‑113 (MQ=255)
                                                         cGGACGCCCGGTTTCCTTGTCCCACACCACCGTGGTTTCGCGTTGGTTGGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTCG‑ACCATGGTGGCGCTTTGTGTGGCGAAAAtct                                                                                                      >  2:37056/1‑137 (MQ=255)
                                                         cGGACGCCCGGTTTCCTTGTCCCACACCACCGTGGTTTCGCGTTGGTTGGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTCG‑ACCATGGTGGCGCTTTGTGTGGCGAAAATCTCCATCGGGTCAt                                                                                          >  4:14685/1‑149 (MQ=255)
                                                         cGGACGCCCGGTTTCCTTGTCCCACACCACCGTGGTTTCGCGTTGGGTGGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTCGTACCATGGTGGCGCTTTGTGTGGCGAAAAtct                                                                                                      <  1:37056/138‑1 (MQ=255)
                                                              gCCCGGTTTCCTTGTCCCACACCACCGTGGTTTCGCGTTGGTTGGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTCG‑ACCATGGTGGCGCTTTGTGTGGCGAAAATCTCCATCGGGTCATGCTc                                                                                      >  3:81249/1‑148 (MQ=255)
                                                              gCCCGGTTTCCTTGTCCCACACCACCGTGGTTTCGCGTTGGTTGGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTCG‑ACCATAGTGGCGCttt                                                                                                                     <  2:183922/117‑1 (MQ=255)
                                                              gCCCGGTTTCCTTCTCCCACACCACCGTGGTTTCGCGTTGGATGGTGATGCCCAGCGCACCGACCTGGGCGTGGCTGATACCCGCCTGTGCAAGGGCCTCG‑ACCATGGTGGCGCTTTGTGTGGCGAAAATCTCCATCGGGTCAt                                                                                          >  5:349982/1‑144 (MQ=255)
                                                              gCCAGGTTTCCTTGTCCCACACCACCGTGGTTTCGCGTTGGTTGTTGATGCCCATCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGTCCTCG‑ACCATGGTGGCGCTTTGTGTGGCGAAAATCTCCATCTGGTCAt                                                                                          <  6:349982/144‑1 (MQ=255)
                                                               cccGGTTTCCTTGTCCCACACCACCGTGGTTTCGCGTTGGTTGGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTCG‑ACCATGGTGGCGCTTTGTGTGGCGAAAATCTCCATCGGGTCATGCTCGa                                                                                    <  1:120663/149‑1 (MQ=255)
                                                                ccGGTTTCCTTGTCCCACACCACCGTGGTTTCGCGTTGGTTGGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTCG‑ACCATGGTGGCGCTTTGTGTGGCGAAAATCTCCATCGGGTCATGCTCGAc                                                                                   >  5:214415/1‑149 (MQ=255)
                                                                      tCCTTGTCCCACACCACCGTGGTTTCGCGTTGGTTGGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCACCTGGGCAAGGGCCTCG‑ACCAtggt                                                                                                                             >  7:138306/1‑101 (MQ=255)
                                                                      tCCTTGTCCCACACCACCGTGGTTTCGCGTTGGTTGGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCACCTGGGCAAGGGCCTCG‑ACCAtggt                                                                                                                             <  8:138306/101‑1 (MQ=255)
                                                                              ccACACCACCGTGGTTTCGCGTTGGTTGGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTCG‑ACCATGGTGGCGCTTTGTGTGGCGAAAATCTCCATCGGGTCATGCTCGACCCAGCCCGCCTGCg                                                                     >  4:195585/1‑149 (MQ=255)
                                                                              ccACACCACCGTGGTTTCGCGTTGGTTGGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTCG‑ACCATGGTGGCGCTTTGTGTGGCGAAAATCTCCATCGGGTCATGCTCGACCCAGCCCGCCTGCg                                                                     >  5:110494/1‑149 (MQ=255)
                                                                              ccACACCACCGTGGTTTCGCGTTGGTTGGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTCG‑ACCATGGTGGCGCTTTGTGTGGCGAAAATCTCCATCGGGTCATGCTCGACCCAGCCCGCCTGCg                                                                     >  2:52741/1‑149 (MQ=255)
                                                                               cacaCCACCGTGGTTTCGCGTTGGTTGGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTCG‑ACCATGGTGGCGCTTTGTGTGGCGAAAATCTCCATCGGGTCATGCTCGACCCAGCCCGCCTGCgg                                                                    <  4:131334/149‑1 (MQ=255)
                                                                                   ccaccGTGGTTTCGCGTTGGTTGGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTCG‑ACCATGGTGGCGCTTTGTGTGGCGAAAATCTCCATCGGGTCATGCTCGACCCAGCCCGCCTGCGGGTaa                                                                <  3:195585/149‑1 (MQ=255)
                                                                                         tGGTTTCGCGTTGGTTGGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTCG‑ACCATGGTGGCGCTTTGTGTGGCGAAAATCTCCATCGGGTCATGCTCGACCCAGCCCGCCTGCGGGTAATGCtgt                                                          <  1:52741/149‑1 (MQ=255)
                                                                                         tGGTTTCGCGTTGGTTGGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTCG‑ACCATGGTGGCGCTTTGTGTGGCGAAAATCTCCATCGGGTCATGCTCGACCCAGCCCGCCTGCGGGTAATGCtgt                                                          <  1:58549/149‑1 (MQ=255)
                                                                                           gTTTCGCGTTGGTTGGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTCG‑ACCATGGTGGCGCTTTGTGTGGCGAAAATCTCCATCGGGTCATGCTCGACCCAGCCCGCCTGCGGGTAATGCTGTGc                                                        <  6:110494/149‑1 (MQ=255)
                                                                                                gcgTTGGTTGGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTCG‑ACCATGGTGGCGCTTTGTGTGGCGAAAATCTCCATCGGGTCATGCTCGACCCAGCACGCCTGCGGGTAATGCTGTGCGAACt                                                   >  8:162474/1‑149 (MQ=255)
                                                                                                  gttggttgGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTCG‑ACCATGGTGGCGCTTTGTGTGGCGAAAATCTCCATCGGGTCAt                                                                                          <  3:259813/108‑1 (MQ=255)
                                                                                                  gttggttgGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTCG‑ACCATGGTGGCGCTTTGTGTGGCGAAAATCTCCATCGGGTCAt                                                                                          >  4:259813/1‑108 (MQ=255)
                                                                                                      gttggtGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTCG‑ACCATGGTGGCGCTTTGTGTGGCGAAAATCTCCATCGGGTCATGCTCGACCCAGCCCGCCTGCGGGTAATGCTGTGCGAACTCGCGCt                                             >  6:42085/1‑149 (MQ=255)
                                                                                                         ggtGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTCG‑ACCATGGTGGCGCTTTGTGTTGCGAAAATCTCCATCGGGTCATGCTCGACCCAGCCCGCCTGCGGGTAATGCTGTGCGAACTCGCGCTgg                                           <  7:162474/148‑1 (MQ=255)
                                                                                                         ggtGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTCG‑ACCATGGTGGCGCTTTGTGTGGCGAAAATCTCCATCGGGTCATGCTCGACCCAGCCCGCCTGCGGGTAATGCTGTGCGAACTCGCGCTgg                                           <  3:14685/148‑1 (MQ=255)
                                                                                                         ggtGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCACCTGGGCAAGGGCCTCG‑ACCATGGTGGCGCTTTGTGTGGCGAAAATCTCCATCGGGTCATGCTCGACCCAGCCCGCCTGCGGGTAATGCTGTGCGAACTCGCGCTgg                                           <  6:315183/148‑1 (MQ=255)
                                                                                                          gtGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTCG‑ACCATGGTGGCGCTTTGTGTGGCGAAAATCTCCATCGGGTCATGCTCGACCCAGCCCGCCTGCGGGTAATGCTGTGCGAACTCGCGCTGGGa                                         <  3:324847/149‑1 (MQ=255)
                                                                                                             aTGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTCG‑ACCATGGTGGCGCTTTGTGTGGCGAAAATCTCCATCGGGTCATGCTCGACCCAGCCCGCCTGCGGGTAATGCTGTGCGAACTCGCGCTGGGAGGt                                      <  6:214415/149‑1 (MQ=255)
                                                                                                               gCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTCG‑ACCATGGTGGCGCTTTGTGTGGCGAAAATCTCCATCGGGTCATGCTCGACCCAGCCCGCCTGc                                                                      >  6:211376/1‑115 (MQ=255)
                                                                                                               gCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTCG‑ACCATGGTGGCGCTTTGTGTGGCGAAAATCTCCATCGGGTCATGCTCGACCCAGCCCGCCTGc                                                                      <  5:211376/115‑1 (MQ=255)
                                                                                                                                                 gCCTGGGCAAGGGCCTCG‑ACCATGGTGGCGCTTTGTGTGGCGAAAATCTCCATCGGGTCATGCTCGACCCAGCCCGCCTGCGGGTAATGCTGTGCGAACTCGCGCTGGGAGGTACCTACAACATTGGCGTCACGGTCGAAAATGATCGc  >  4:238025/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
GAGCTGAGCACAGATCTCGGTGCTGCGGCGGCACTGCCAGACAATGGCGTTGTACACCGGACGCCCGGTTTCCTTGTCCCACACCACCGTGGTTTCGCGTTGGTTGGTGATGCCCAGCGCAGCGACCTGGGCGTGGCTGATACCCGCCTGGGCAAGGGCCTCG‑ACCATGGTGGCGCTTTGTGTGGCGAAAATCTCCATCGGGTCATGCTCGACCCAGCCCGCCTGCGGGTAATGCTGTGCGAACTCGCGCTGGGAGGTACCTACAACATTGGCGTCACGGTCGAAAATGATCGC  >  NC_002947/1234929‑1235222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑