Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,243,064 C→G 7.4% intergenic (‑180/‑45) PP_1083 ← / → tsaA (2Fe‑2S)‑binding protein/peroxiredoxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,243,0640CG 7.4% 153.7 / 4.1 54intergenic (‑180/‑45)PP_1083/tsaA(2Fe‑2S)‑binding protein/peroxiredoxin
Reads supporting (aligned to +/- strand):  ref base C (28/22);  new base G (2/2);  total (30/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.29e-01

CTTAATGTTAATGAGAATGCTTGTCAAAGATCTTTCTGAGGGTGCTGTGCTTGAGCGACCGGTGGTCGCTTTGTCGACGGAGTCGGCCTGTCATGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCACCCTCATCAGGAGATTACAATGAGCGTACTCGTTGGTAAGAAAGCCCCCGATTTCACCGTTCCGGCCGTGCTGGGCAATGGCGAGATCGTCGATAGCTTCAACCTGGCCTCGGCCATCAAG  >  NC_002947/1242923‑1243210
                                                                                                                                             |                                                                                                                                                  
cTTAATGTTAATGAGAATGCTTGTCAAAGATCTTTCTGAGGGTGCTGTGCTTGAGAGACCGGTGGTCGCTTTGTCGACGGAGTCGGCCTGTCATGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGc                                                                                                                                             >  3:158924/1‑149 (MQ=255)
 ttaatgttaatgAGAATGCTTGTCAAAGATCTTTCTGAGGGTGCTGTGCTTGAGCGACCGGTGGTCGCTTTGTCGACGGAGTCGGCCTGTCATGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGcc                                                                                                                                            >  4:333225/1‑149 (MQ=255)
   aatgttaatgAGAATGCTTGTCAAAGATCTTTCTGAGGGTGCTGTGCTTGAGCGACCGGTGGTCGCTTTGTCGACGGAGTCGGCCTGTCATGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGcc                                                                                                                                            <  8:246838/147‑1 (MQ=255)
   aatgttaatgAGAATGCTTGTCAAAGATCTTTCTGAGGGTGCTGTGCTTGAGCGACCGGTGGTCGCTTTGTCGACGGAGTCGGCCTGTCATGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCg                                                                                                                                          >  3:104464/1‑149 (MQ=255)
         aatgAGAATGCTTGTCAAAGATCTTTCTGAGGGTGCTGTGCTTGAGCGACCGGTGGTCGCTTTGTCGACGGAGTCGGCCTGTCATGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCGGCAAAc                                                                                                                                    >  8:243174/1‑149 (MQ=255)
            gagaATGCTTGTCAAAGATCTTTCTGAGGGTGCTGTGCTTGAGCGACCGGTGGTCGCTTTGTCGACGGAGTCGGCCTGTCATGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCCAGACGGGCTTGCCCGACatc                                                                                                                                     <  8:129926/145‑3 (MQ=255)
            gagaATGCTTGTCAAAGATCTTTCTGAGGGTGCTGTGCTTGAGCGACCGGTGGTCGCTTTGTCGACGGAGTCGGCCTGTCATGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCCAGACGGGCTTGCCCGACatc                                                                                                                                     >  7:129926/1‑143 (MQ=255)
             agaATGCTTGTCAAAGATCTTTCTGAGGGTGCTGTGCTTGAGCGACCGGTGGTCGCTTTGTCGACGGAGTCGGCCTGTCATGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGt                                                                                                                                 <  3:333225/148‑1 (MQ=255)
               aaTGCTTGTCAAAGATCTTTCTGAGGGTGCTGTGCTTGAGCGACCGGTGGTCGCTTTGTCGACGGAGTCGGCCTGTCATGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGtttt                                                                                                                              >  5:40049/1‑149 (MQ=255)
                    ttGTCAAAGATCTTTCTGAGGGTGCTGTGCTTGAGCGACCGGTGGTCGCTTTGTCGACGGAGTCGGCCTGTCATGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCAcc                                                                                                                         >  5:76491/1‑149 (MQ=255)
                      gTCAAAGATCTTTCTGAGGGTGCTGTGCTTGAGCGACCGGTGGTCGCTTTGTCGACGGAGTCGGCCTGTCATGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCAccc                                                                                                                        <  6:40049/148‑1 (MQ=255)
                       tCAAAGATCTTTCTGAGGGTGCTGTGCTTGAGCGACCGGTGGTCGCTTTGTCGACGGAGTCGGCCTGTCATGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGccc                                                                                                                                                    >  4:146350/1‑119 (MQ=255)
                       tCAAAGATCTTTCTGAGGGTGCTGTGCTTGAGCGACCGGTGGTCGCTTTGTCGACGGAGTCGGCCTGTCATGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGccc                                                                                                                                                    <  3:146350/119‑1 (MQ=255)
                       tCAAAGATCTTTCTGAGGGTGCTGTGCTTGAGCGACCGGTGGTCGCTTTGTCGACGGAGTCGGCCTGTCATGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCACCCt                                                                                                                       >  8:97341/1‑148 (MQ=255)
                          aaGATCTTTCTGAGGGTGCTGTGCTTGAGCGACCGGTGGTCGCTTTGTCGACGGAGTCGGCCTGTCATGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCACCCtcat                                                                                                                    >  3:220948/1‑148 (MQ=255)
                                  tCTGAGGGTGCTGTGCTTGAGCGACCGGTGGTCGCTTTGTCGACGGAGTCGGCCTGTCATGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCACCCTCATCAGGAGAt                                                                                                            >  7:304719/1‑148 (MQ=255)
                                      aGGGTGCTGTGCTTGAGCGACCGGTGGTCGCTTTGTCGACGGAGTCGGCCTGTCATGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCACCCTCATCAGGAGATTa                                                                                                          <  5:282368/146‑1 (MQ=255)
                                         gtgctgtgctTGAGCGACCGGTGGTCGCTTTGTCGACGGAGTCGGCCTGTCATGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCACCCTCATCAGGAGATTACAATGa                                                                                                    <  4:104464/149‑1 (MQ=255)
                                          tgctgtgctTGAGCGACCGGTGGTCGCTTTGTCGACGGAGTCGGCCTGTCATGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCCAGACGGGCTTGCCCga                                                                                                                                         >  2:74790/1‑110 (MQ=255)
                                          tgctgtgctTGAGCGACCGGTGGTCGCTTTGTCGACGGAGTCGGCCTGTCATGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCCAGACGGGCTTGCCCga                                                                                                                                         <  1:74790/111‑2 (MQ=255)
                                             tgtgctTGAGCGACCGGTGGTCGCTTTGTCGACGGAGTCGGCCTGTCATGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCACCCTCATCAGGAGATTACAATGAGCg                                                                                                 >  3:214733/1‑148 (MQ=255)
                                                      gCGACCGGTGGTCGCTTTGTCGACGGAGTCGGCCTGTCATGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCACCCTCATCAGGAGATTACAATGAGCGTACTCGTTg                                                                                        <  4:158924/148‑1 (MQ=255)
                                                      gCGACCGGTGGTCGCTTTGTCGACGGAGTCGGCCTGTCATGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCACCCTCATCAGGAGATTACAATGAGCGTACTCGTTg                                                                                        <  8:304719/148‑1 (MQ=255)
                                                             gtggtCGCTTTGTCGACGGAGTCGGCCTGTCATGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCACCCTCATCAGGAGATTACAATGAGCGTACTCGTTGGTAAGaaa                                                                                <  6:76491/149‑1 (MQ=255)
                                                                    cTTTGTCGACGGAGTCGGCCTGTCATGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCACCCTCATCAGga                                                                                                               <  2:265357/111‑1 (MQ=255)
                                                                    cTTTGTCGACGGAGTCGGCCTGTCATGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCACCCTCATCAGga                                                                                                               >  1:265357/1‑111 (MQ=255)
                                                                        gTCGACGGAGTCGGCCTGTCATGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCACCCTCATCAGGAGATTACAATGAGCGTACTCGTTGGt                                                                                      >  6:308872/1‑132 (MQ=255)
                                                                        gTCGACGGAGTCGGCCTGTCATGACAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCACCCTCATCAGGAGATTACAATGAGCGTACTCGTTGGt                                                                                      <  5:308872/132‑1 (MQ=255)
                                                                         tCGACGGAGTCGGCCTGTCATGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCACCCTCATCAGGAGATTACAATGAGCGTACTCGTTGGTAAGAAAGCCCCCGAttt                                                                     >  2:244233/1‑148 (MQ=255)
                                                                         tCGACGGAGTCGGCCTGTCATGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCACCCTCATCAGGAGATTACAATGAGCGTACTCGTTGGTAAGAAAGCCCCCGAttt                                                                     >  2:272997/1‑148 (MQ=255)
                                                                           gACGGAGTCGGCCTGTCATGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCACCCTCATCAgg                                                                                                                >  6:137179/1‑103 (MQ=255)
                                                                           gACGGAGTCGGCCTGTCATGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCACCCTCATCAgg                                                                                                                <  5:137179/103‑1 (MQ=255)
                                                                           gACGGAGTCGGCCTGTCATGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCACCCTCATCAGGAGATTACAATGAGCGTACTCGTTGGTAAGAAAGCCCCCGATTTCa                                                                   <  7:97341/148‑1 (MQ=255)
                                                                           gACGGAGTCGGCCTGTCATGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCACCCTCATCAGGAGATTACAATGAGCGTACTCGTTGGTAAGAAAGCCCCCGATTTCAc                                                                  >  1:157977/1‑149 (MQ=255)
                                                                           gACGGAGTCGGCCTGTCATGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCACCCTCATCAGGAGATTACAATGAGCGTACTCGTTGGTAAGAAAGCCCCCGATTTCAc                                                                  >  3:140394/1‑149 (MQ=255)
                                                                              ggAGTCGGCCTGTCATGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCACCCTCATCAGGAGATTACAATGAGCGTACTCGTTGGTAAGAAAGCCCCCGATTTCACCGt                                                               >  8:72863/1‑149 (MQ=255)
                                                                                aGTCGGCCTGTCATGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCACCCTCATCAGGAGATTACAATGAGCGTACTCGTTGGTAAGAAAGCCCCCGAttt                                                                     <  1:129982/141‑1 (MQ=255)
                                                                                aGTCGGCCTGTCATGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCACGGCAAACGGTTTTTCACCCTCATCAGGAGATTACAATGAGCGTACTCGTTGGTAAGAAAGCCCCCGAttt                                                                     >  2:129982/1‑141 (MQ=255)
                                                                                  tCGGCCTGTCATGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCACCCTCATCAGGAGATTACAATGAGCGTACTCGTTGGTAAGAAAGCCCCCGATTTCACCGTTCCg                                                           <  2:157977/149‑1 (MQ=255)
                                                                                       cTGTCATGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCACCCTCATCAGGAGATTACAATGAGCGTACTCGTTGGTAAGAAAGCCCCCGATTTCACCGTTCCGGCCGt                                                      <  7:72863/149‑1 (MQ=255)
                                                                                           cATGAGAAGCCGTGTTTGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCACCCTCATCAGGAGTTTACAATGAGCGTACTCGTTGGTAAGAAAGCCGCCTATTTCACCGTTCCGGCCGTGCt                                                   <  4:220948/148‑1 (MQ=255)
                                                                                            aTGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCACCCTCATCAGGAGATTACAATGAGCGTACTCGTTGGTAAGa                                                                                  <  7:202587/116‑1 (MQ=255)
                                                                                            aTGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCACCCTCATAAGCAGATTACAATGAGCGTACTCTTTGGTAAGa                                                                                  >  8:202587/1‑116 (MQ=255)
                                                                                               agaAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCACCCTCATCAGGAGATTACAATGAGCGTACTCGTTGGGAAGAAAGCCCCCGATTTCACCGTTCCGGCCGTGCTGGGc                                               >  8:65360/1‑148 (MQ=255)
                                                                                                    ccGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCACCCTCATCAGGAGATTACAATGAGCGTACTCGTTGGTATGAAAGCCCCCGATTTCACCGTTCCGGCCGTGCTGGGCAATGGc                                         <  1:244233/149‑1 (MQ=255)
                                                                                                      gtgtATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCACCCTCATCAGGAGATTACAATGAGCGTACTCGTTGGTAAGAAAGCCCCCGATTTCACCGTTCCGGCCGTGCTGGGCAATGGCga                                       >  3:282434/1‑149 (MQ=255)
                                                                                                         tATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCACCCTCATCAGGAGATTACAATGAGCGTACTCGTTGGTAAGAAAGCCCCCGATTTCACCGTTCCGGCCGTGCTGGGCAATGGc                                         >  7:32351/1‑144 (MQ=255)
                                                                                                           tgatgGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCACCCTCATCAGGAGATTACAATGAGCGTACTCGTTGGTAAGAAAGCCCCCGATTTCACCGTTCCGGcc                                                        >  3:173341/1‑127 (MQ=255)
                                                                                                           tgatgGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCACCCTCATCAGGAGATTACAATGAGCGTACTCGTTGGTAAGAAAGCCCCCGATTTCACCGTTCCGGcc                                                        <  4:173341/127‑1 (MQ=255)
                                                                                                                gTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCACCCTCATCAGGAGATTACAATGAGCGTACTCGTTGGTAAGAAAGCCCCCGATTTCACCGTTCCGGCCGTGCTGGGCAATGGCGAGATCGTCGa                              <  2:221824/148‑1 (MQ=255)
                                                                                                                           ttgGATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCACCCTCATCAGGAGATTACAATGAGCGTACTCGTTGGTAAGAAAGCCCCCGATTTCACCGTTCCGGCCGTGCTGGGCAATGGCGAGATCGTCGATAGCTTCAACCt                  >  6:200922/1‑149 (MQ=255)
                                                                                                                              gATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCACCCTCATCAGGAGATTACAATGAGCGTACTCGTTGGTAAGAAAGCCCCCGATTTCACCGTTCCGGCCGTGCTGGGCAATGGCGAGATCGTCGATAGCTTCAACCTGGc               <  8:32351/149‑1 (MQ=255)
                                                                                                                                   cGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCACCCTCATCAGGAGATTACAATGAGCGTACTCGTTGGTAAGAAAGCCCCCGATTTCACCGTTCCGGCCGTGCTGGGCAATGGCGAGAtcgtcg                               >  3:239436/1‑128 (MQ=255)
                                                                                                                                   cGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCACCCTCATCAGGAGATTACAATGAGCGTACTCGTTGGTAAGAAAGCCCCCGATTTCACCGTTCCGGCCGTGCTGGGCAATGGCGAGATCGTCGATAGCTTCAACCTGGCCTCgg          >  6:313285/1‑149 (MQ=255)
                                                                                                                                   cGGGCAAGCACGTCTGGCCCGGCAAACGGTTTTTCACCCTCATCAGGAGATTACAATGAGCGTACTCGTTGGTAAGAAAGCCCCCGATTTCACCGTTCCGGCCGTGCTGGGCAATGGCGAGAtcgtcg                               <  4:239436/128‑1 (MQ=255)
                                                                                                                                     ggCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCACCCTCATCAGGAGATTACAATGAGCGTACTCGTTGGTAAGAAAGCCCCCGATTTCACCGTTCCGGCCGTGCTGGGCAATGGCGAGATCGTCGATAGCTTCAACCTGGCCTCGGc         >  6:216506/1‑148 (MQ=255)
                                                                                                                                           cccGTCTGGCCCGGCAAACGGTTTTTCACCCTCATCAGGAGATTACAATGAGCGTACTCGTTGGTAAGAAAGCCCCCGATTTCACCGTTCCGGCCGTGCTGGGCAATGGCGAGATCGTCGATAGCTTCAACCTGGCCTCGGCCATCAAg  <  4:214733/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                  
CTTAATGTTAATGAGAATGCTTGTCAAAGATCTTTCTGAGGGTGCTGTGCTTGAGCGACCGGTGGTCGCTTTGTCGACGGAGTCGGCCTGTCATGAGAAGCCGTGTATGATGGTCGGCCTTTGTTGGATGTCGGGCAAGCCCGTCTGGCCCGGCAAACGGTTTTTCACCCTCATCAGGAGATTACAATGAGCGTACTCGTTGGTAAGAAAGCCCCCGATTTCACCGTTCCGGCCGTGCTGGGCAATGGCGAGATCGTCGATAGCTTCAACCTGGCCTCGGCCATCAAG  >  NC_002947/1242923‑1243210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑