Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,273,323 C→T 6.6% intergenic (+114/+54) PP_1113 → / ← PP_1114 pyridoxal‑phosphate dependent enzyme family protein/SEC‑C domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,273,3230CT 6.6% 180.4 / 2.2 60intergenic (+114/+54)PP_1113/PP_1114pyridoxal‑phosphate dependent enzyme family protein/SEC‑C domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (29/27);  new base T (2/2);  total (31/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

GCAAACCGGCGAAAGGCCGCGTAGCCACCTGTGTCAGGCTCATGATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTC  >  NC_002947/1273186‑1273469
                                                                                                                                         |                                                                                                                                                    
gCAAACCGGCGAAAGGCCGCGTAGCCACCTGTGTCAGGCTCATGATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCca                                                                                                                                           <  4:36725/149‑1 (MQ=255)
  aaaCCGGCGAAAGGCCGCGTAGCCACCTGTGTCAGGCTCATGATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCcaca                                                                                                                                         <  4:181762/149‑1 (MQ=255)
      cGGCGAAAGGCCGCGTAGCCACCTGTGTCAGGCTCATGATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAgtgt                                                                                                                                     <  4:125616/149‑1 (MQ=255)
          gAAAGGCCGCGTAGCCACCTGTGTCAGGCTCATGATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTc                                                                                                                                   >  5:22653/1‑147 (MQ=255)
             aGGCCGCGTAGCCACCTGTGTCAGGCTCATGATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGc                                                                                                                               >  8:191641/1‑148 (MQ=255)
                 cgcgTAGCCACCTGTGTCAGGCTCATGATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAt‑c‑t                                                                                                                        >  8:329409/1‑149 (MQ=255)
                     tAGCCACCTGTGTCAGGCTCATGATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGATAAGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAt‑c‑tct                                                                                                                      >  1:285625/1‑147 (MQ=255)
                       gCCACCTGTGTCAGGCTCATGATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAg                                                                                                                   <  7:191641/148‑1 (MQ=255)
                           ccTGTGTCAGGCTCATGATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGAtttt                                                                                                              >  7:283443/1‑149 (MQ=255)
                              gtgtCAGGCTCATGATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTAt                                                                                                           >  2:268651/1‑149 (MQ=255)
                                gtCAGGCTCATGATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTa                                                                                                            <  1:57523/146‑1 (MQ=255)
                                gtCAGGCTCATGATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTa                                                                                                            >  2:57523/1‑146 (MQ=255)
                                 tCAGGCTCATGATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATcgc                                                                                                        >  8:166343/1‑149 (MQ=255)
                                   aGGCTCATGATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATcgcg                                                                                                       <  8:283443/148‑1 (MQ=255)
                                   aGGCTCATGATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGt                                                                                                      <  5:214460/149‑1 (MQ=255)
                                     gCTCATGATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGt                                                                                                      >  1:313566/1‑147 (MQ=255)
                                       tCATGATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGt                                                                                                  <  5:256025/149‑1 (MQ=255)
                                         aTGATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTc                                                                                                 <  7:329409/148‑1 (MQ=255)
                                           gATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCa                                                                                                    >  1:129234/1‑143 (MQ=255)
                                           gATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCa                                                                                                    <  2:129234/143‑1 (MQ=255)
                                             tCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATc                                                                                             <  6:22653/148‑1 (MQ=255)
                                                   ggCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGcc                                                                                      <  6:290783/149‑1 (MQ=255)
                                                          aCGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCAc                                                                               >  5:238345/1‑149 (MQ=255)
                                                            ggAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCGACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGGCAGTCGATCAGGCGCCGTGGCActt                                                                             >  5:118614/1‑149 (MQ=255)
                                                                aCTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTg                                                                         <  6:238345/149‑1 (MQ=255)
                                                                 cTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGt                                                                        <  3:308079/149‑1 (MQ=255)
                                                                 cTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGt                                                                        <  7:166343/149‑1 (MQ=255)
                                                                    tgGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTAct                                                                     >  7:152151/1‑149 (MQ=255)
                                                                     gggagcgggcgcgcccgcgaacacgggcgaagcccgtgccatccaccgcgtcgcctgcTTCGCGGGCTTGCCCGCTCCCACAGTTTCCGTGCCAG‑CCTCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCActtc                                                                            >  4:183366‑M1/59‑142 (MQ=255)
                                                                     gggagcgggcgcgcccgcgaacacgggcgaagcccgtgccatccaccgcgtcgcctgcTTCGCGGGCTTGCCCGCTCCCACAGTTTCCGTGCCAG‑CCTCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCActtc                                                                            <  3:183366‑M1/84‑1 (MQ=255)
                                                                       gagcgggcgcgcccgcgaacacgggcgaagcccgtgccatccaccgcgtcgcctgcTTCGCGGGCTTGCCCGCTCCCACAGTTTCCGTGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGGGGCACTTCTTGTActtct                                                                  <  6:10202‑M1/93‑1 (MQ=255)
                                                                       gAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTActtct                                                                  >  5:359236/1‑149 (MQ=255)
                                                                        aGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTActtct                                                                  >  6:244945/1‑148 (MQ=255)
                                                                           gggCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCActtc                                                                            <  7:36994/135‑1 (MQ=255)
                                                                           gggCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCACGCCCCGAGGCAcatc                                                                            >  8:36994/1‑135 (MQ=255)
                                                                                 gCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTAcc                                                        >  3:89509/1‑149 (MQ=255)
                                                                                    cgcgAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACttcttc                                                                <  6:135750/138‑1 (MQ=255)
                                                                                    cgcgAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACttcttc                                                                >  5:135750/1‑138 (MQ=255)
                                                                                    cgcgAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGc                                                            >  7:178417/1‑142 (MQ=255)
                                                                                    cgcgAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGc                                                            <  8:178417/142‑1 (MQ=255)
                                                                                       gAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCgcacgg                                                  >  7:113684/1‑149 (MQ=255)
                                                                                           cAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCActtc                                                                            <  5:164832/119‑1 (MQ=255)
                                                                                           cAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCActtc                                                                            >  6:164832/1‑119 (MQ=255)
                                                                                                 aCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCg                                        <  2:285625/149‑1 (MQ=255)
                                                                                                     ggTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTAc                                    <  5:244945/149‑1 (MQ=255)
                                                                                                         gATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGc                                 >  1:314901/1‑148 (MQ=255)
                                                                                                           gaacACGGGCGAAGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCaa                              >  2:120919/4‑149 (MQ=255)
                                                                                                             gCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAAc                             <  2:314901/148‑1 (MQ=255)
                                                                                                             gCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACg                            >  6:140013/1‑149 (MQ=255)
                                                                                                                     ttCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTActtc                                                                   <  2:315135/102‑1 (MQ=255)
                                                                                                                     ttCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTActtc                                                                   >  1:315135/1‑102 (MQ=255)
                                                                                                                     ttCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAg                    >  7:267094/1‑149 (MQ=255)
                                                                                                                     ttCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCACAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAg                    >  2:305860/1‑149 (MQ=255)
                                                                                                                       cGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAg                    <  8:152151/147‑1 (MQ=255)
                                                                                                                          ccGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCATGC‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCg                >  1:68235/1‑149 (MQ=255)
                                                                                                                           cGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGCGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCCCGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGtt              >  5:174216/1‑149 (MQ=255)
                                                                                                                             tgtTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGc            <  4:89509/149‑1 (MQ=255)
                                                                                                                              cTTCGCGGGCTTGCCCGCTCCCACAGTTTCCGTGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGc            >  5:10202‑M1/2‑148 (MQ=255)
                                                                                                                                  gcgGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTAt        <  5:140013/148‑1 (MQ=255)
                                                                                                                                  gcgGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATc       <  5:184511/149‑1 (MQ=255)
                                                                                                                                       cgctcccgctccCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTc  <  6:118614/149‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                                    
GCAAACCGGCGAAAGGCCGCGTAGCCACCTGTGTCAGGCTCATGATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTC  >  NC_002947/1273186‑1273469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑