Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,273,341	T→C	5.7%	intergenic (+132/+36)	PP_1113 → / ← PP_1114	pyridoxal‑phosphate dependent enzyme family protein/SEC‑C domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,273,341	0	T	C	5.7%	204.0 / 2.1	70	intergenic (+132/+36)	PP_1113/PP_1114	pyridoxal‑phosphate dependent enzyme family protein/SEC‑C domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (35/31);  new base C (2/2);  total (37/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01

GAAAGGCCGCGTAGCCACCTGTGTCAGGCTCATGATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGG  >  NC_002947/1273196‑1273482                                                                                                                                                  |                                                                                                                                                gAAAGGCCGCGTAGCCACCTGTGTCAGGCTCATGATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTc                                                                                                                                                >  5:22653/1‑147 (MQ=255)    aGGCCGCGTAGCCACCTGTGTCAGGCTCATGATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGc                                                                                                                                            >  8:191641/1‑148 (MQ=255)        cgcgTAGCCACCTGTGTCAGGCTCATGATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAt‑c‑t                                                                                                                                     >  8:329409/1‑149 (MQ=255)            tAGCCACCTGTGTCAGGCTCATGATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGATAAGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAt‑c‑tct                                                                                                                                   >  1:285625/1‑147 (MQ=255)              gCCACCTGTGTCAGGCTCATGATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAg                                                                                                                                <  7:191641/148‑1 (MQ=255)                  ccTGTGTCAGGCTCATGATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGAtttt                                                                                                                           >  7:283443/1‑149 (MQ=255)                     gtgtCAGGCTCATGATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTAt                                                                                                                        >  2:268651/1‑149 (MQ=255)                       gtCAGGCTCATGATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTa                                                                                                                         <  1:57523/146‑1 (MQ=255)                       gtCAGGCTCATGATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTa                                                                                                                         >  2:57523/1‑146 (MQ=255)                        tCAGGCTCATGATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATcgc                                                                                                                     >  8:166343/1‑149 (MQ=255)                          aGGCTCATGATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATcgcg                                                                                                                    <  8:283443/148‑1 (MQ=255)                          aGGCTCATGATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGt                                                                                                                   <  5:214460/149‑1 (MQ=255)                            gCTCATGATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGt                                                                                                                   >  1:313566/1‑147 (MQ=255)                              tCATGATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGt                                                                                                               <  5:256025/149‑1 (MQ=255)                                aTGATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTc                                                                                                              <  7:329409/148‑1 (MQ=255)                                  gATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCa                                                                                                                 >  1:129234/1‑143 (MQ=255)                                  gATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCa                                                                                                                 <  2:129234/143‑1 (MQ=255)                                    tCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATc                                                                                                          <  6:22653/148‑1 (MQ=255)                                          ggCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGcc                                                                                                   <  6:290783/149‑1 (MQ=255)                                                 aCGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCAc                                                                                            >  5:238345/1‑149 (MQ=255)                                                   ggAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCGACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGGCAGTCGATCAGGCGCCGTGGCActt                                                                                          >  5:118614/1‑149 (MQ=255)                                                       aCTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTg                                                                                      <  6:238345/149‑1 (MQ=255)                                                        cTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGt                                                                                     <  3:308079/149‑1 (MQ=255)                                                        cTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGt                                                                                     <  7:166343/149‑1 (MQ=255)                                                           tgGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTAct                                                                                  >  7:152151/1‑149 (MQ=255)                                                            gggagcgggcgcgcccgcgaacacgggcgaagcccgtgccatccaccgcgtcgcctgcTTCGCGGGCTTGCCCGCTCCCACAGTTTCCGTGCCAG‑CCTCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCActtc                                                                                         >  4:183366‑M1/59‑142 (MQ=255)                                                            gggagcgggcgcgcccgcgaacacgggcgaagcccgtgccatccaccgcgtcgcctgcTTCGCGGGCTTGCCCGCTCCCACAGTTTCCGTGCCAG‑CCTCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCActtc                                                                                         <  3:183366‑M1/84‑1 (MQ=255)                                                              gagcgggcgcgcccgcgaacacgggcgaagcccgtgccatccaccgcgtcgcctgcTTCGCGGGCTTGCCCGCTCCCACAGTTTCCGTGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGGGGCACTTCTTGTActtct                                                                               <  6:10202‑M1/93‑1 (MQ=255)                                                              gAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTActtct                                                                               >  5:359236/1‑149 (MQ=255)                                                               aGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTActtct                                                                               >  6:244945/1‑148 (MQ=255)                                                                  gggCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCActtc                                                                                         <  7:36994/135‑1 (MQ=255)                                                                  gggCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCACGCCCCGAGGCAcatc                                                                                         >  8:36994/1‑135 (MQ=255)                                                                        gCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTAcc                                                                     >  3:89509/1‑149 (MQ=255)                                                                           cgcgAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACttcttc                                                                             >  5:135750/1‑138 (MQ=255)                                                                           cgcgAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACttcttc                                                                             <  6:135750/138‑1 (MQ=255)                                                                           cgcgAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGc                                                                         >  7:178417/1‑142 (MQ=255)                                                                           cgcgAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGc                                                                         <  8:178417/142‑1 (MQ=255)                                                                              gAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCgcacgg                                                               >  7:113684/1‑149 (MQ=255)                                                                                  cAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCActtc                                                                                         <  5:164832/119‑1 (MQ=255)                                                                                  cAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCActtc                                                                                         >  6:164832/1‑119 (MQ=255)                                                                                        aCGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCg                                                     <  2:285625/149‑1 (MQ=255)                                                                                            ggTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTAc                                                 <  5:244945/149‑1 (MQ=255)                                                                                                gATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGc                                              >  1:314901/1‑148 (MQ=255)                                                                                                  gaacACGGGCGAAGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCaa                                           >  2:120919/4‑149 (MQ=255)                                                                                                    gCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAAc                                          <  2:314901/148‑1 (MQ=255)                                                                                                    gCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACg                                         >  6:140013/1‑149 (MQ=255)                                                                                                            ttCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTActtc                                                                                >  1:315135/1‑102 (MQ=255)                                                                                                            ttCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTActtc                                                                                <  2:315135/102‑1 (MQ=255)                                                                                                            ttCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAg                                 >  7:267094/1‑149 (MQ=255)                                                                                                            ttCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCACAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAg                                 >  2:305860/1‑149 (MQ=255)                                                                                                              cGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAg                                 <  8:152151/147‑1 (MQ=255)                                                                                                                 ccGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCATGC‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCg                             >  1:68235/1‑149 (MQ=255)                                                                                                                  cGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGCGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCCCGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGtt                           >  5:174216/1‑149 (MQ=255)                                                                                                                    tgtTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGc                         <  4:89509/149‑1 (MQ=255)                                                                                                                     cTTCGCGGGCTTGCCCGCTCCCACAGTTTCCGTGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGc                         >  5:10202‑M1/2‑148 (MQ=255)                                                                                                                         gcgGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTAt                     <  5:140013/148‑1 (MQ=255)                                                                                                                         gcgGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATc                    <  5:184511/149‑1 (MQ=255)                                                                                                                              cgctcccgctccCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTc               <  6:118614/149‑1 (MQ=255)                                                                                                                                 gcCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCgcacgg                                                               >  6:160672/1‑98 (MQ=255)                                                                                                                                 gcCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCgcacgg                                                               <  5:160672/98‑1 (MQ=255)                                                                                                                                    cGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCtggtg         <  1:120919/149‑1 (MQ=255)                                                                                                                                       tCCCACAGTGTTCGATCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGcc      >  6:110839/1‑149 (MQ=255)                                                                                                                                       tCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTc               >  7:97235/1‑140 (MQ=255)                                                                                                                                       tCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTc               <  8:97235/140‑1 (MQ=255)                                                                                                                                       tCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGcc      >  5:125232/1‑149 (MQ=255)                                                                                                                                         ccACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGcc      <  6:125232/147‑1 (MQ=255)                                                                                                                                         ccACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGt    <  6:359236/149‑1 (MQ=255)                                                                                                                                         ccACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGt    >  4:164728/1‑149 (MQ=255)                                                                                                                                         ccACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGt    >  8:48600/1‑149 (MQ=255)                                                                                                                                           acaGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTgg  <  7:48600/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                GAAAGGCCGCGTAGCCACCTGTGTCAGGCTCATGATCCAGAGGCTGGCACGGAAACTGTGGGAGCGGGCAAGCCCGCGAAGCAGGCGACGCGGTGGATGGCACGGGCTTCGCCCGTGTTCGCGGGCGCGCCCGCTCCCACAGTGTTCGAGCCAT‑C‑TCTCAGATTTTTATCGCGTCAGTCGATCAGGCGCCGTGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGG  >  NC_002947/1273196‑1273482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑