Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,307,440 C→G 6.0% G60R (GGC→CGC)  livK ← branched‑chain amino acids ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,307,4400CG 6.0% 215.6 / 3.4 66G60R (GGC→CGC) livKbranched‑chain amino acids ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (35/27);  new base G (2/2);  total (37/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.54e-01

GGTGGAGCTGGAGCACAGGTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAATGGCTGGCTACCCCGGC  >  NC_002947/1307303‑1307578
                                                                                                                                         |                                                                                                                                          
ggTGGAGCTGGAGCACAGGTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCGGGCGGCGTGGa                                                                                                                                 <  7:212167/149‑1 (MQ=255)
          gagcACAGGTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGtt                                                                                                                                   >  5:2698/1‑137 (MQ=255)
          gagcACAGGTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGtt                                                                                                                                   <  6:2698/137‑1 (MQ=255)
          gagcACAGGTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGa                                                                                                                        <  3:111443/148‑1 (MQ=255)
            gcACAGGTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCGGGCGGCGTGGat                                                                                                                                >  1:32213/1‑138 (MQ=255)
            gcACAGGTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCGGGCGGCGTGGat                                                                                                                                <  2:32213/138‑1 (MQ=255)
               caGGTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATgg                                                                                                                     <  5:254320/146‑1 (MQ=255)
               caGGTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATgg                                                                                                                     >  6:254320/1‑146 (MQ=255)
                 ggTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGgcttgc                                                                                                                >  6:172681/1‑149 (MQ=255)
                  gTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCGATCCACGCCGCCCGCGGCGTTGATATCCt                                                                                                                           >  5:307120/1‑137 (MQ=255)
                  gTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCt                                                                                                                           <  6:307120/137‑1 (MQ=255)
                  gTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGgcttgct                                                                                                               >  1:162065/1‑149 (MQ=255)
                  gTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGgcttgct                                                                                                               >  7:125318/1‑149 (MQ=255)
                        gACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGgcgc                                                                                                         <  8:125318/149‑1 (MQ=255)
                                     aCGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCa                                                                                             <  3:189749/148‑1 (MQ=255)
                                       gCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGCTATCCTTGAGGGCTTGCTAGGCGCCGa                                                                                                      >  7:235994/1‑137 (MQ=255)
                                          gtcgtTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGc                                                                                       <  2:162065/149‑1 (MQ=255)
                                           tcgtTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGcc                                                                                      <  5:172681/149‑1 (MQ=255)
                                             gtTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGc                                                                                              >  6:235969/1‑139 (MQ=255)
                                             gtTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGc                                                                                              <  5:235969/139‑1 (MQ=255)
                                                gACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGgcttgctt                                                                                                              >  4:40586/1‑120 (MQ=255)
                                                   cACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGTCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTggg                                                                              >  6:200291/1‑149 (MQ=255)
                                                   cACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTggg                                                                              >  1:68047/1‑149 (MQ=255)
                                                      tttGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCa                                                                           >  2:184619/1‑149 (MQ=255)
                                                                   gCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCtt                                                              >  2:235413/1‑149 (MQ=255)
                                                                      aCGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTggg                                                           <  1:235413/149‑1 (MQ=255)
                                                                          ccTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCg                                                       >  4:52137/1‑149 (MQ=255)
                                                                            tGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGa                                                          >  6:247804/1‑144 (MQ=255)
                                                                            tGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGa                                                          <  5:247804/144‑1 (MQ=255)
                                                                              tttAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCgg                                                      >  6:344771/1‑146 (MQ=255)
                                                                              tttAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCgg                                                      <  5:344771/146‑1 (MQ=255)
                                                                                   ggTCGCAGGCGTCCTCGAATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATACTTGATGGCTTGCTTGGCGCCGATGAAATGCATGTCTCCGTACAGGGTCACGGGACCCGTCTTGGGCCCGGCGATGCCg                                              >  8:256606/1‑149 (MQ=255)
                                                                                    gTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCCCGTGAcccagta                                                               >  1:304552/1‑127 (MQ=255)
                                                                                    gTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCCCGTGAcccagta                                                               <  2:304552/131‑5 (MQ=255)
                                                                                    gTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTg                                                             >  5:316895/1‑133 (MQ=255)
                                                                                    gTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTg                                                             <  6:316895/133‑1 (MQ=255)
                                                                                       gCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGTCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTgg                                                            <  2:320844/131‑1 (MQ=255)
                                                                                       gCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTgg                                                            >  1:320844/1‑131 (MQ=255)
                                                                                           gCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGAt                                      <  3:52137/149‑1 (MQ=255)
                                                                                           gCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGAt                                      <  2:68047/149‑1 (MQ=255)
                                                                                            cgtcgtcgtATTCCTTGGCTTCGAGCATCTTGTCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATg                                     <  5:200291/149‑1 (MQ=255)
                                                                                             gtcgtcgtATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATgg                                    <  4:57675/149‑1 (MQ=255)
                                                                                                 tcgtATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGAt                                      >  6:58714/1‑143 (MQ=255)
                                                                                                 tcgtATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGAt                                      <  5:58714/143‑1 (MQ=255)
                                                                                                 tcgtATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATg                                     >  7:292573/1‑144 (MQ=255)
                                                                                                 tcgtATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATg                                     <  8:292573/144‑1 (MQ=255)
                                                                                                    tATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTg                                        >  3:328963/1‑138 (MQ=255)
                                                                                                    tATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTg                                        <  4:328963/138‑1 (MQ=255)
                                                                                                       tCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATcggcgg                           >  4:150347/1‑148 (MQ=255)
                                                                                                        ccTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTg                                                                                               <  7:124076/79‑1 (MQ=255)
                                                                                                        ccTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTg                                                                                               >  8:124076/1‑79 (MQ=255)
                                                                                                         cTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCa                         >  4:80990/1‑148 (MQ=255)
                                                                                                         cTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCa                         >  4:97722/1‑148 (MQ=255)
                                                                                                         cTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCa                         >  3:98035/1‑148 (MQ=255)
                                                                                                          ttGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCaaa                       >  1:24293/1‑149 (MQ=255)
                                                                                                          ttGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCaaa                       >  6:351412/1‑149 (MQ=255)
                                                                                                            ggCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCaaa                       <  5:351412/147‑1 (MQ=255)
                                                                                                            ggCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAAc                      >  8:282910/1‑148 (MQ=255)
                                                                                                                            ccATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATcggcgg                           <  2:160699/127‑1 (MQ=255)
                                                                                                                             cATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAATGGCTGGCTACCCCg    >  6:24176/1‑149 (MQ=255)
                                                                                                                                caaCGCCGCGGGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAATGGCTGGCTACCCCGGc  >  6:322640/3‑148 (MQ=255)
                                                                                                                                 cACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAAtggctgg            <  4:180301/137‑1 (MQ=255)
                                                                                                                                 cACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAAtggctgg            >  3:180301/1‑137 (MQ=255)
                                                                                                                                 cACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAATGGCTGGCTACCCCGGc  >  6:120431/1‑147 (MQ=255)
                                                                                                                                  aCGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGTGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAAtggct              <  5:233281/134‑1 (MQ=255)
                                                                                                                                  aCGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGTGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAAtggct              >  6:233281/1‑134 (MQ=255)
                                                                                                                                     ccgcccgcGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATcggcgg                           >  7:192496/1‑118 (MQ=255)
                                                                                                                                     ccgcccgcGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATcggcgg                           <  8:192496/118‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                          
GGTGGAGCTGGAGCACAGGTGGCCGACCACGAACTTGACGCCGTCGTTGACCACTTTGTTGGCGACTGCCACGGCCTGTTTAGGGTCGCAGGCGTCGTCGTATTCCTTGGCTTCGAGCATCTTGCCATCCACGCCGCCCGCGGCGTTGATATCCTTGATGGCTTGCTTGGCGCCGATGAACTGCATGTCGCCGTACTGGGTCACGGGACCCGTCTTGGGACCGGCGATGCCGATCTTGATGGTATCGGCGGCAAACGAATGGCTGGCTACCCCGGC  >  NC_002947/1307303‑1307578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑