Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,344,115 A→C 7.3% C8G (TGC→GGC)  PP_1168 ← TRAP dicarboxylate transporter subunit DctQ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,344,1150AC 7.3% 167.6 / 3.8 55C8G (TGC→GGC) PP_1168TRAP dicarboxylate transporter subunit DctQ
Reads supporting (aligned to +/- strand):  ref base A (24/27);  new base C (2/2);  total (26/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

GGCGAAGATGCCCCACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGC‑GTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGTCGACGGTAAT  >  NC_002947/1343969‑1344250
                                                                                                                                                   |                                                                                                                                       
ggCGAAGATGCCCCACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGC‑GTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACt                                                                                                                                       >  7:80488/1‑149 (MQ=255)
  cGAAGATGCCCCACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGC‑GTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGc                                                                                                                                     <  1:327822/149‑1 (MQ=255)
   gAAGATGCCCCACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGC‑GTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGTGCGGCGCCCGCCGCCCTGcc                                                                                                                                    <  8:245104/149‑1 (MQ=255)
        tGCCCCACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGC‑GTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGt                                                                                                                               <  8:4324/149‑1 (MQ=255)
             cACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGC‑GTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTg                                                                                                                                      <  1:296661/137‑1 (MQ=255)
             cACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGC‑GTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTg                                                                                                                                      >  2:296661/1‑137 (MQ=255)
               cGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGC‑GTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTgg                                                                                                                        <  1:39725/149‑1 (MQ=255)
               cGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGC‑GTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTgg                                                                                                                        >  6:107799/1‑149 (MQ=255)
                 ggATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGC‑GTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGc                                                                                                                      <  8:80488/149‑1 (MQ=255)
                         aGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGC‑GTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGggcgg                                                                                                              >  3:248757/1‑149 (MQ=255)
                               aTGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGC‑GTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGATGTTa                                                                                                        >  3:68736/1‑149 (MQ=255)
                                         tCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGC‑GTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCg                                                                                              >  4:161785/1‑149 (MQ=255)
                                                gCCCGCGATCCAGATGCAACTGCGGTACAGC‑GTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGt                                                                                                          >  8:129257/1‑130 (MQ=255)
                                                gCCCGCGATCCAGATGCAACTGCGGTACAGC‑GTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGt                                                                                                          <  7:129257/130‑1 (MQ=255)
                                                  ccGCTATCCATATGCAACTGCGTTACAGC‑GTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCGTCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGc                                                                                     <  4:68736/149‑1 (MQ=255)
                                                    gcgATCCAGATGCAACTGCGGTACAGCGGTGTCGTTCACGCTCAGGAAAAGCGATTACATAGGCATCACAAAACCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTg                                                                                                                         >  1:187100/1‑112 (MQ=255)
                                                    gcgATCCAGATGCAACTGCGGTACAGC‑GTGTCGTTCACGCTCAGTAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTg                                                                                                                         <  2:187100/111‑1 (MQ=255)
                                                       aTCCAGATGCAACTGCGGTACAGC‑GTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTtcat                                                                                <  3:205248/149‑1 (MQ=255)
                                                       aTCCAGATGCAACTGCGGTACAGC‑GTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTtcat                                                                                <  3:299533/149‑1 (MQ=255)
                                                         ccAGATGCAACTGCGGTACAGC‑GTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGa                                                                                                                                  >  3:302214/1‑97 (MQ=255)
                                                         ccAGATGCAACTGCGGTACAGC‑GTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGa                                                                                                                                  <  4:302214/97‑1 (MQ=255)
                                                           aGATGCAACTGCGGTACAGC‑GTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAgg                                                                            >  2:27042/1‑149 (MQ=255)
                                                               gCAACTGCGGTACAGC‑GTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAgg                                                                            <  2:235250/145‑1 (MQ=255)
                                                               gCAACTGCGGTACAGC‑GTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAgg                                                                            >  1:235250/1‑145 (MQ=255)
                                                                         tACAGC‑GTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTgcgc                                                              >  3:287122/1‑149 (MQ=255)
                                                                          aCAGC‑GTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTAc                                                                                                       >  4:335739/1‑107 (MQ=255)
                                                                            aGC‑GTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGcc                                                           >  3:96379/1‑149 (MQ=255)
                                                                                     gTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTAttttt                                                   <  4:248757/149‑1 (MQ=255)
                                                                                      ttCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTtcatc                                                                               <  2:223243/120‑1 (MQ=255)
                                                                                         aCGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTcgcg                                               >  8:75541/1‑149 (MQ=255)
                                                                                           gCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTcgcg                                               <  8:174370/147‑1 (MQ=255)
                                                                                           gCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTcgcg                                               >  7:174370/1‑147 (MQ=255)
                                                                                           gCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTa                                             >  8:5298/1‑149 (MQ=255)
                                                                                             tCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTcg                                                 >  2:132395/1‑143 (MQ=255)
                                                                                             tCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTcg                                                 <  1:132395/143‑1 (MQ=255)
                                                                                                ggAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGaa                                        <  7:75541/149‑1 (MQ=255)
                                                                                                 gAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAg                                       >  2:102644/1‑149 (MQ=255)
                                                                                                     aGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGt                                   <  1:27042/149‑1 (MQ=255)
                                                                                                             cATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTg                                                                 <  3:257102/111‑1 (MQ=255)
                                                                                                             cATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTg                                                                 >  4:257102/1‑111 (MQ=255)
                                                                                                             cATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGcgtcg                            <  5:107799/148‑1 (MQ=255)
                                                                                                             cATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGcgtc                             >  1:187189/1‑147 (MQ=255)
                                                                                                             cATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGcgtc                             <  2:187189/147‑1 (MQ=255)
                                                                                                              aTAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGcgtcg                            >  5:124979/1‑147 (MQ=255)
                                                                                                                    aTCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAAc                      >  6:254136/1‑147 (MQ=255)
                                                                                                                    aTCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAAc                      <  5:254136/147‑1 (MQ=255)
                                                                                                                       aCCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGctt                 <  4:96379/149‑1 (MQ=255)
                                                                                                                       aCCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGct                  <  4:287122/148‑1 (MQ=255)
                                                                                                                          aaaGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGcttctt              >  7:146513/1‑149 (MQ=255)
                                                                                                                              cGGCAGTGCGGCGCCCGCCGCCCTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGTCg          >  3:99939/1‑149 (MQ=255)
                                                                                                                                 cAGTGCGGCGCCCGCCGCCCTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGTCGACg       >  8:110693/1‑149 (MQ=255)
                                                                                                                                     gcggcgGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGTCGACGGTaa   <  7:5298/149‑1 (MQ=255)
                                                                                                                                      cggcggGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGTCGACGGTAAt  <  3:161785/149‑1 (MQ=255)
                                                                                                                                      cggcgcCCGCCGCCCTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGTCGACGGTAAt  <  7:110693/149‑1 (MQ=255)
                                                                                                                                               ccgcACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGcgtcgt                           <  4:4742/115‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                       
GGCGAAGATGCCCCACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGC‑GTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGTCGACGGTAAT  >  NC_002947/1343969‑1344250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑