Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,368,197 G→T 9.3% intergenic (+26/‑157) PP_1193 → / → PP_1194 membrane protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,368,1970GT 9.3% 121.2 / 2.3 42intergenic (+26/‑157)PP_1193/PP_1194membrane protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (18/20);  new base T (2/2);  total (20/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01

GTTGGTGGTGAACCTGGTGACGATCACGGCAACCATGTGGGTGGCGAACCCGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGATTGCGGTTTGCGTGGGATTAATTCCCGCGTCT  >  NC_002947/1368051‑1368329
                                                                                                                                                  |                                                                                                                                    
gTTGGTGGTGAACCTGGTGACGATCACGGCAACCATGTGGGTGGCGAACCCGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCg                                                                                                                                    >  1:177532/1‑149 (MQ=255)
           aCCTGGTGACGATCACGGCAACCATGTGGGTGGCGAACCCGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTg                                                                                                                         <  6:90097/149‑1 (MQ=255)
                      aTCACGGCAACCATGTGGGTGGCGAACCCGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCAAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTAt                                                                                                                      <  2:130523/141‑1 (MQ=255)
                      aTCACGGCAACCATGTGGGTGGCGAACCCGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCAAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTAt                                                                                                                      >  1:130523/1‑141 (MQ=255)
                        cACGGCAACCATGTGGGTGGCGAACCCGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACg                                                                                                                                <  4:140337/129‑1 (MQ=255)
                        cACGGCAACCATGTGGGTGGCGAACCCGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACg                                                                                                                                >  3:140337/1‑129 (MQ=255)
                          cGGCAACCATGTGGGTGGCGAACCCGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTg                                                                                                          >  4:48610/1‑149 (MQ=255)
                          cGGCAACCATGTGGGTGGCGAACCCGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTg                                                                                                          <  2:46347/149‑1 (MQ=255)
                          aggCAACCATGTGGGTGGCGAACCCGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCGTCCGCTGGGTTTTGTATGGGcca                                                                                                                <  1:263162/142‑3 (MQ=38)
                              aaCCATGTGGGTGGCGAACCCGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCt                                                                                                      >  3:271045/1‑149 (MQ=255)
                                 cATGTGGGTGGCGAACCCGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTc                                                                                                   <  2:177532/149‑1 (MQ=255)
                                                 ccGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGc                                                                                                        >  7:245023/1‑128 (MQ=255)
                                                 ccGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGc                                                                                                        <  8:245023/128‑1 (MQ=255)
                                                         gACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCaa                                                                           >  3:221312/1‑149 (MQ=255)
                                                            cACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTgg                                                                                                         >  8:153285/1‑116 (MQ=255)
                                                            cACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTgg                                                                                                         <  7:153285/116‑1 (MQ=255)
                                                            cACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAAt                                                                          >  2:343789/1‑147 (MQ=255)
                                                                   ttCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTc                                                                                                   >  3:329788/1‑115 (MQ=255)
                                                                   ttCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTc                                                                                                   <  4:329788/115‑1 (MQ=255)
                                                                      aTGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTCCCTTTATCACGTCATTTCCAATGAGCGCTGACAg                                                              >  4:115297/1‑149 (MQ=255)
                                                                           ggcggcGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCtttt                                                         <  3:48610/149‑1 (MQ=255)
                                                                                 gAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCGTCCGCTGGGTTTTGTAt                                                                                                                      >  8:8796/1‑82 (MQ=37)
                                                                                           aTGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCgg                                         >  4:165012/1‑149 (MQ=255)
                                                                                                aacaGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGatat                                    <  4:271045/149‑1 (MQ=255)
                                                                                                  cagcagAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTg                                                                                                          <  4:210136/77‑1 (MQ=255)
                                                                                                  cagcagAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGa                                  <  4:221312/149‑1 (MQ=255)
                                                                                                     cataaGCTGATTGGCGATGACCCGAAGTGGCACATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTGTTGCCTTTATCACGTCATTTCCAATGAGCTCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGATTg                               <  3:115297/146‑1 (MQ=255)
                                                                                                        aaGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGATTGCgg                            >  7:136754/1‑149 (MQ=255)
                                                                                                                  gcgcTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGATTGCGGTTTGCGTgg                   <  1:343789/148‑1 (MQ=255)
                                                                                                                    gcTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGATTGCGGTTTGCGTGGGAt                >  5:110614/1‑149 (MQ=255)
                                                                                                                    gcTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGATTGCGGTTTGCGTGGGAt                <  8:136754/149‑1 (MQ=255)
                                                                                                                          ccGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGATTGCGGTTTGCGTGGGATTAATTc          >  3:54885/1‑149 (MQ=255)
                                                                                                                            gAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGATTGCGGTTTGCGTGGGATTAATTccc        <  4:54885/149‑1 (MQ=255)
                                                                                                                               gTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGATTGCGGTTTGCGTGGGATTAATTCCcgcg     <  3:165012/149‑1 (MQ=255)
                                                                                                                                tGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGATTGCGGTTTGCGTGGGATTAATTCCCGCGt    <  6:110614/149‑1 (MQ=255)
                                                                                                                                  gCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGATTGCGGTTTGCGTGGGATTAATTCCCGCGTCt  >  3:103699/1‑149 (MQ=255)
                                                                                                                                      aTACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATAACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCggg                                        >  4:24318/1‑107 (MQ=255)
                                                                                                                                       tACAAAACCCGTCCGCTGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGATTGCGGTTTGCGTGGGATTAAtt           <  5:344355/135‑1 (MQ=255)
                                                                                                                                       tACAAAACCCGTCCGCTGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGATTGCGGTTTGCGTGGGATTAAtt           >  6:344355/1‑135 (MQ=255)
                                                                                                                                          taatcccTGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGATTGCGGTTTGCGTGGGAt                <  4:298364/123‑1 (MQ=255)
                                                                                                                                          aaaaCCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGATTGCg                             <  7:99729/114‑1 (MQ=255)
                                                                                                                                          aaaaCCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGATTGCg                             >  8:99729/1‑114 (MQ=255)
                                                                                                                                                  |                                                                                                                                    
GTTGGTGGTGAACCTGGTGACGATCACGGCAACCATGTGGGTGGCGAACCCGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGATTGCGGTTTGCGTGGGATTAATTCCCGCGTCT  >  NC_002947/1368051‑1368329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑