Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,383,461	G→A	7.7%	L524L (CTG→TTG)	proS ←	proline‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,383,461	0	G	A	7.7%	187.9 / 2.8	64	L524L (CTG→TTG)	proS	proline‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (30/29);  new base A (3/2);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTCGGTGCGGTGCTTGTACTCCAGGTTGCCTTCGGCCAGGCCGCGATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGG  >  NC_002947/1383315‑1383606                                                                                                                                                   |                                                                                                                                                  tCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTCGGTGCGGTGCTTGTACTCCAGGTTGCCTTCGGCCAGGCCGCGATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGc                                                                                                                                                  <  4:217814/148‑1 (MQ=255)        cgAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTCGGTGCGGTGCTTGTACTCCAGGTTGCCTTCGGCCAGGCCGCGATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGCTCCATGTc                                                                                                                                          <  2:143522/149‑1 (MQ=255)         gAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTCGGTGCGGTGCTTGTACTCCAGGTTGCCTTCGGCCAGGCCGCGATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGCTCCATGTCg                                                                                                                                         >  6:64285/1‑149 (MQ=255)                   cTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTCGGTGCGGTGCTTGTACTCCAGGTTGCCTTCGGCCAGGCCGCGATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGCTcc                                                                                                                                               <  1:178147/133‑1 (MQ=255)                   cTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTCGGTGCGGTGCTTGTACTCCAGGTTGCCTTCGGCCAGGCCGCGATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGCTcc                                                                                                                                               >  2:178147/1‑133 (MQ=255)                     gCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTCGGTGCGGTGCTTGTACTCCAGGTTGCCTTCGGCCAGGCCGCGATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGCTCCATGTCg                                                                                                                                         >  5:94600/1‑137 (MQ=255)                     gCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTCGGTGCGGTGCTTGTACTCCAGGTTGCCTTCGGCCAGGCCGCGATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGCTCCATGTCg                                                                                                                                         <  6:94600/137‑1 (MQ=255)                     gCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTCGGTGCGGTGCTTGTACTCCAGGTTGCCTTCGGCCAGGCCGCGATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGCTCCATGTCGGCAAACTTGATg                                                                                                                             >  8:191679/1‑149 (MQ=255)                       aGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTCGGTGCGGTGCTTGTACTCCAGGTTGCCTTCGGCCAGGCCGCGATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGCTCCATGTCGGCAAACTTGATGcc                                                                                                                           >  7:176386/1‑149 (MQ=255)                         gAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTCGGTGCGGTGCTTGTACTCCAGGTTGCCTTCGGCCAGGCCGCGATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGCTCCATGTCGGCAAACTTGATg                                                                                                                             >  8:233896/1‑145 (MQ=255)                         gAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTCGGTGCGGTGCTTGTACTCCAGGTTGCCTTCGGCCAGGCCGCGATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGCTCCATGTCGGCAAACTTGATg                                                                                                                             <  7:233896/145‑1 (MQ=255)                          aaGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTCGGTGCGGTGCTTGTACTCCAGGTTGCCTTCGGCCAGGCCGCGATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGCTCCATGTCGGCAAACTTGATGCCTgg                                                                                                                        <  7:191679/149‑1 (MQ=255)                           aGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTCGGTGCGGTGCTTGTACTCCAGGTTGCCTTCGGCCAGGCCGCGATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGCTCCATGTCGGCAAACTTGATGCCTggg                                                                                                                       >  8:300408/1‑149 (MQ=255)                               cAGCACTTCCTGGCGCGGCAACGCCTGGGCGTCCTGCTCGGTGCGGTGCTTGTACTCCAGGTTGCCTTCGGCCAGGCCGCGATCGCTGACGACGATGCGGTGTGGGCGGCCGATCGGCTCCATGTCGGCAAACTTGATGCCTGGGCTgg                                                                                                                   <  4:84454/149‑1 (MQ=255)                                        aTTGAGCGGCAACGCCTGGGCGTCCTGCTCGGTGCGGTGCTTGTACTCCAGGTTGCCTTCGGCCAGGCCGCGATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGt                                                                                                          >  3:10284/1‑149 (MQ=255)                                                gCAACGCCTGGGCGTCCTGCTCGGTGCGGTGCTTGTACTCCAGGTTGCCTTCGGCCAGGCCGCGATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTCGCGgtcg                                                                                                  >  3:156024/1‑149 (MQ=255)                                                         gggCGTCCTGCTCGGTGCGGTGCTTGTACTCCAGGTTGCCTTCGGCCAGGCCGCGATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCtt                                                                                                            <  5:268921/130‑1 (MQ=255)                                                         gggCGTCCTGCTCGGTGCGGTGCTTGTACTCCAGGTTGCCTTCGGCCAGGCCGCGATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCtt                                                                                                            >  6:268921/1‑130 (MQ=255)                                                                 tGCTCGGTGCGGTGCTTGTACTCCAGGTTGCCTTCGGCCAGGCCGCGATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCcag                                                                                             <  7:24353/137‑1 (MQ=255)                                                                 tGCTCGGTGCGGTGCTTGTACTCCAGGTTGCCTTCGGCCAGGCCGCGATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCcag                                                                                             >  8:24353/1‑137 (MQ=255)                                                                  gCTCGGTGCGGTGCTTGTACTCCAGGTTGCCTTCGGCCAGGCCGCGATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGttt                                                                                                                >  7:95406/1‑117 (MQ=255)                                                                  gCTCGGTGCGGTGCTTGTACTCCAGGTTGCCTTCGGCCAGGCCGCGATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGttt                                                                                                                <  8:95406/117‑1 (MQ=255)                                                                   cTCGGTGCGGTGCTTGTACTCCAGGTTGCCTTCGGCCAGGCCGCGATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAg                                                                                <  4:156024/148‑1 (MQ=255)                                                                       gtgcggtgcTTGTACTCCAGGTTGCCTTCGGCCAGGCCGCGATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCcggc                                                                           >  1:8547/1‑149 (MQ=255)                                                                           ggtgcTTGTACTCCAGGTTGCCTTCGGCCAGGCCGCGATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTcg                                                                                                        >  1:136988/1‑116 (MQ=255)                                                                           ggtgcTTGTACTCCAGGTTGCCTTCGGCCAGGCCGCGATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTcg                                                                                                        <  2:136988/116‑1 (MQ=255)                                                                           ggtgcTTGTACTCCAGGTTGCCTTCGGCCAGGCCGCGATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGca                                                                       <  5:64285/149‑1 (MQ=255)                                                                               cTTGTACTCCAGGTTGCCTTCGGCCAGGCCGCGATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGcagtca                                                                   <  8:176386/149‑1 (MQ=255)                                                                                     cTCCAGGTTGCCTTCGGCCAGGCCGCGATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCgg                                                             >  6:54693/1‑149 (MQ=255)                                                                                        cAGGTTGCCTTCGGCCAGGCCGCGATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGCTCAATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCa                                                           >  7:225746/1‑148 (MQ=255)                                                                                         aGGTTGCCTTCGGCCAGGCCGCGATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATa                                                         <  5:294347/149‑1 (MQ=255)                                                                                               ccTTCGGCCAGGCCGCGATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCtt                                                   <  2:8547/149‑1 (MQ=255)                                                                                                cTTCGGCCAGGCCGCGATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCa                                                           >  5:147524/1‑140 (MQ=255)                                                                                                cTTCGGCCAGGCCGCGATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCa                                                           <  6:147524/140‑1 (MQ=255)                                                                                                       cAGGCCGCGATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGcagt                                                                     >  6:360424/1‑123 (MQ=255)                                                                                                       cAGGCCGCGATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGcagt                                                                     <  5:360424/123‑1 (MQ=255)                                                                                                        aGGCCGCGATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACa                                                       >  1:248604/1‑136 (MQ=255)                                                                                                        aGGCCGCGATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGACGGCGGCAGTCAGTTCGGCATACAGATTGTCGGTTGc                                          >  7:144878/1‑149 (MQ=255)                                                                                                        aGGCCGCGATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGCCCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACa                                                       <  2:248604/136‑1 (MQ=255)                                                                                                          gCCGCGATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCtt                                        >  3:216510/1‑149 (MQ=255)                                                                                                              cgATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTcgcg                                    <  8:225746/149‑1 (MQ=255)                                                                                                                     tGACGACGATGCGGTGTGGGATGCCGATCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTcgc                                     <  2:84410/141‑1 (MQ=255)                                                                                                                     tGACGACGATGCGGTGTGGGATGCCGATCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTcgc                                     >  1:84410/1‑141 (MQ=255)                                                                                                                       acgacgATGCGGTGTGGGATGCCGATCAACTCCATGTCGGCAAACTTGATGCCTGGTCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCgg                                   <  2:69834/141‑1 (MQ=255)                                                                                                                       acgacgATGCGGTGTGGGATGCCGATCAACTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCgg                                   >  1:69834/1‑141 (MQ=255)                                                                                                                       acgacgATGCGGTGTGGGATGCCGATCAACTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCgg                                   >  1:64896/1‑141 (MQ=255)                                                                                                                       acgacgATGCGGTGTGGGATGCCGATCAACTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCgg                                   <  2:64896/141‑1 (MQ=255)                                                                                                                         gacgaTGCGGTGTGGGATGCCGATCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCgg                         >  8:185474/1‑149 (MQ=255)                                                                                                                         gacgaTGCGGTGTGGGATGCCGATCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCgg                         >  8:180250/1‑149 (MQ=255)                                                                                                                            gaTGCGGTGTGGGATGCCGATCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGttt                      <  1:271985/149‑1 (MQ=255)                                                                                                                            gaTGCGGTGTGGGATGCCGATCAGCTCCATGTCGGCAAACTTGATGCCT‑GGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTc                     >  7:68041/1‑149 (MQ=255)                                                                                                                              tGCGGTGTGGGATGCCGATCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCg                    >  2:43356/1‑149 (MQ=255)                                                                                                                               cccacctgGTATGCCGATCAGCTCCATGTCGGCAAACTTGATGCCTGGGCGGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATATAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTc                     <  6:320536/141‑1 (MQ=255)                                                                                                                                cgtggtgGTATGCCTATCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGTTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCTGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGt                   <  8:144878/144‑1 (MQ=255)                                                                                                                                cGGTGTGGGATGCCGATCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGt                   <  4:10284/148‑1 (MQ=255)                                                                                                                                         aTGCCGATCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTgg         >  6:356461/1‑149 (MQ=255)                                                                                                                                          tGCCGATCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTcg                                      >  7:46896/1‑119 (MQ=255)                                                                                                                                          tGCCGATCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTcg                                      <  8:46896/119‑1 (MQ=255)                                                                                                                                            ccGATCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGAc      <  7:180250/149‑1 (MQ=255)                                                                                                                                            ccGATCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGGCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGAc      <  7:185474/149‑1 (MQ=255)                                                                                                                                               aTCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAg   >  7:262829/1‑149 (MQ=255)                                                                                                                                               aTCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAg   >  5:134891/1‑149 (MQ=255)                                                                                                                                               aTCAACTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGGGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAg   >  2:279271/1‑149 (MQ=255)                                                                                                                                                tCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAgg  <  5:54693/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  TCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTCGGTGCGGTGCTTGTACTCCAGGTTGCCTTCGGCCAGGCCGCGATCGCTGACGACGATGCGGTGTGGGATGCCGATCAGCTCCATGTCGGCAAACTTGATGCCTGGGCTGGTTTTCTTGTCGCGGTCGTCCAGCAGCACTTCATAGCCGGCGGCAGTCAGTTCGGCATACAGCTTGTCGGTTGCTTCGCGGACCACGTCGGTTTCGTAGCGCAGTGGGACCAGG  >  NC_002947/1383315‑1383606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑