Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,392,066	A→G	8.3%	intergenic (+97/‑18)	aspS → / → PP_1214	aspartate‑‑tRNA ligase/transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,392,066	0	A	G	8.3%	130.5 / 2.7	48	intergenic (+97/‑18)	aspS/PP_1214	aspartate‑‑tRNA ligase/transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (21/23);  new base G (2/2);  total (23/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CCCTGCGCGAACTGCACATCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACATGGATGCGCCTTTGCGTTTCGACG‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAGGTCTTCACCAAGTGGATCCGTGAACTGACGGTCGCTGCCAAGCAGGGTGGCCCGGATCCGG  >  NC_002947/1391920‑1392213                                                                                                                                                    |                                                                                                                                                    cccTGCGCGAACTGCACATCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACATGGATGCGCCTTTGCGTTTCGACG‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCaaa                                                                                                                                                   <  8:316713/149‑1 (MQ=255) cccTGCGCGAACTGCACATCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACATGGATGCGCCTTTGCGTTTCGACG‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCaaa                                                                                                                                                   <  1:68210/149‑1 (MQ=255)  ccTGCGCGAACTGCACATCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACATGGATGCGCCTTTGCGTTTCGACGACAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCaaa                                                                                                                                                   <  1:266245/149‑1 (MQ=255)       gcgAACTGCACATCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACATGGATGCGCCTTTGCGTTTCGACG‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTc                                                                                                                                             <  7:313944/149‑1 (MQ=255)        cgAACTGCACATCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACATGGATGCGCCTTTGCGTTTCGACG‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCg                                                                                                                                            <  6:120452/149‑1 (MQ=255)        cgAACTGCACATCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACATGGATGCGCCTTTGCGTTTCGACG‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCg                                                                                                                                            <  2:128460/149‑1 (MQ=255)            cTGCACATCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACATGGATGCGCCTTTGCGTTTCGACG‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGt                                                                                                                                        <  4:342954/149‑1 (MQ=255)                acaTCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACATGGATGCGCCTTTGCGTTTCGACG‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCa                                                                                                                                                     <  8:41592/132‑1 (MQ=255)                acaTCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACATGGATGCGCCTTTGCGTTTCGACG‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCa                                                                                                                                                     >  7:41592/1‑132 (MQ=255)                acaTCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACATGGATGCGCCTTTGCGTTTCGACG‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGt                                                                                                                                     >  8:1009/1‑148 (MQ=255)                 caTCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACATGGATGCGCCTTTGCGTTTCGACG‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGtt                                                                                                                                    >  6:151376/1‑148 (MQ=255)                 caTCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACATGGATGCGCCTTTGCGTTTCGACG‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGtt                                                                                                                                    <  5:151376/148‑1 (MQ=255)                       aCTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACATGGATGCGCCTTTGCGTTTCGACG‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTg                                                                                                                             <  8:8913/149‑1 (MQ=255)                                  aGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACATGGATGCGCCTTTGCGTTTCGACG‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCg                                                                                                                                            >  6:109780/1‑123 (MQ=255)                                   gACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACATGGATGCGCCTTTGCGTTTCGACG‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGt                                                                                                                 >  5:128592/1‑149 (MQ=255)                                    aCCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACATGGATGCGCCTTTGCGTTTCGACG‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTg                                                                                                                >  8:262864/1‑149 (MQ=255)                                      cAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACATGGATGCGCCTTTGCGTTTCGACG‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCaaa                                                                                                                                                   <  2:317401/112‑1 (MQ=255)                                      cAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACATGGATGCGCCTTTGCGTTTCGACG‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCaaa                                                                                                                                                   >  1:317401/1‑112 (MQ=255)                                      cAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACATGGATGCGCCTTTGCGTTTCGACG‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTggg                                                                                                              >  7:320430/1‑149 (MQ=255)                                            cGAGTAAGCAGTCCCCGGGCGCATCCACATGGATGCGCCTTTGCGTTTCGACG‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTc                                                                                                                      >  6:196015/1‑135 (MQ=255)                                            cGAGTAAGCAGTCCCCGGGCGCATCCACATGGATGCGCCTTTGCGTTTCGACG‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTc                                                                                                                      <  5:196015/135‑1 (MQ=255)                                                tAAGCAGTCCCCGGGCGCATCCACATGGATGCGCCTTTGCGTTTCGACG‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCaa                                                                                                    <  8:320430/149‑1 (MQ=255)                                                   gCAGTCCCCGGGCGCATCCACATGGATGCGCCTTTGCGTTTCGACG‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCGAAGGATTCGGa                                                                                                                                          >  7:161019/1‑108 (MQ=255)                                                   gCAGTCCCCGGGCGCATCCACATGGATGCGCCTTTGCGTTTCGACG‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCGAAGGATTCGGa                                                                                                                                          <  8:161019/108‑1 (MQ=255)                                                   gCAGTCCCCGGGCGCATCCACATGGATGCGCCTTTGCGTTTCGACG‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCGAAGGATTCGGa                                                                                                                                          <  2:274982/108‑1 (MQ=255)                                                   gCAGTCCCCGGGCGCATCCACATGGATGCGCCTTTGCGTTTCGACG‑CAGGTTTTTTCCAGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCGAAGGATTCGGa                                                                                                                                          >  1:274982/1‑108 (MQ=255)                                                         cccGGGCGCATCCACATGGATGCGCCTTTGCGTTTCGACG‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCa                                                                                            >  6:116716/1‑148 (MQ=255)                                                              gcgcATCCATGTGGATGCGCCTTTGCGTTTCGACG‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAAc                                                                                                         >  4:101132/1‑130 (MQ=255)                                                              gcgcATCCATGTGGATGCGCCTTTGCGTTTCGACG‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAAc                                                                                                         <  3:101132/130‑1 (MQ=255)                                                                  aTCCACATGGATGCGCCTTTGCGTTTCGACG‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAgcgc                                                                                    >  1:142133/1‑147 (MQ=255)                                                                       caTGGATGCGCCTTTGCGTTTCGACG‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAAc                                                                                                         <  4:171625/121‑1 (MQ=255)                                                                       caTGGATGCGCCTTTGCGTTTCGACG‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAAc                                                                                                         >  3:171625/1‑121 (MQ=255)                                                                            aTGCGCCTTTGCGTTTCGACG‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAag                                                                        <  5:116716/149‑1 (MQ=255)                                                                              gcgcCTTTGCGTTTCGACG‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTgg                                                                                                               <  8:227519/108‑1 (MQ=255)                                                                                gcCTTTGCGTTTCGACG‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAg                                                                                                                  >  4:308202/1‑103 (MQ=255)                                                                                gcCTTTGCGTTTCGACG‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAagagag                                                                    <  7:262864/149‑1 (MQ=255)                                                                                  cTTTGCGTTTCGACG‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAaga                                                                                        >  1:296255/1‑127 (MQ=255)                                                                                  cTTTGCGTTTCGACG‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAaga                                                                                        <  2:296255/127‑1 (MQ=255)                                                                                      gCGTTTCGACG‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAg                                                               <  7:1009/148‑1 (MQ=255)                                                                                        gTTTCGACG‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAg                                                               >  1:19931/1‑146 (MQ=255)                                                                                        gTTTCGACG‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAg                                                               <  2:19931/146‑1 (MQ=255)                                                                                                g‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAGGTCTTCACCa                                                     >  2:325423/1‑148 (MQ=255)                                                                                                   aGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAGGt                                                             >  8:170261/1‑138 (MQ=255)                                                                                                   aGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAGGt                                                             <  7:170261/138‑1 (MQ=255)                                                                                                    ggTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAGGTCTTCACCAAGTg                                                 >  6:342545/1‑149 (MQ=255)                                                                                                            cccgtcccgCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAGGTCTTCACCAAGt                                                  <  4:81550/140‑1 (MQ=255)                                                                                                            cccgtcccgCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAGGTCTTCACCAAGt                                                  >  3:81550/1‑140 (MQ=255)                                                                                                            cccgtcccgCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAGGTCTTCACCAAGTGGATCCGt                                          <  1:325423/148‑1 (MQ=255)                                                                                                               gtcccgCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAg                                                               <  1:135395/124‑1 (MQ=255)                                                                                                                    gCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAGGTCTTCACCAAGTGGATCCGTGAACTGACg                                 >  4:245536/1‑149 (MQ=255)                                                                                                                        catgacggggcggggcCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAGGTCTTCACCAAGTGGATCCGTGAACTGACGGTCg                             >  2:10531/7‑149 (MQ=255)                                                                                                                          tCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGc                                                                            >  5:222711/1‑100 (MQ=255)                                                                                                                          tCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAGGTCTTCACCAAGTGGATCCGTGAACTGACGGTCGCt                           <  2:142133/149‑1 (MQ=255)                                                                                                                            acggggcgggacCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAGGTCTTCACCAAGTGGATCCGTGAACTGACGGTCGCTGc                         <  2:228508/147‑1 (MQ=255)                                                                                                                                            tgtgATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAGGTCTTCACCAAGTGGATCCGTGAACTGACGGTCGCTGCCAAGCAGGGTGGccc          <  6:128592/148‑1 (MQ=255)                                                                                                                                                    aaaGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAGGTCTTCACCAAGTGGATCCGTGAACTGACGGTCGCt                           <  6:58859/123‑1 (MQ=255)                                                                                                                                                    aaaGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAGGTCTTCACCAAGTGGATCCGTGAACTGACGGTCGCt                           >  5:58859/1‑123 (MQ=255)                                                                                                                                                    aaaGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAGGTCTTCACCAAGTGGATCCGTGAACTGACGGTCGCTGCCAAGCAGGGTGGCCCGGATCCgg  >  6:227384/1‑148 (MQ=255)                                                                                                                                                    |                                                                                                                                                    CCCTGCGCGAACTGCACATCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACATGGATGCGCCTTTGCGTTTCGACG‑CAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAGGTCTTCACCAAGTGGATCCGTGAACTGACGGTCGCTGCCAAGCAGGGTGGCCCGGATCCGG  >  NC_002947/1391920‑1392213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑