Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,416,874 T→C 9.4% L41P (CTG→CCG)  PP_1239 → metallo‑beta‑lactamase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,416,8740TC 9.4% 138.8 / 5.2 53L41P (CTG→CCG) PP_1239metallo‑beta‑lactamase family protein
Reads supporting (aligned to +/- strand):  ref base T (23/25);  new base C (2/3);  total (25/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

AGCGCTATTCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCC  >  NC_002947/1416726‑1417018
                                                                                                                                                       |                                                                                                                                                
aGCGCTATTCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCgct                                                                                                                                                  >  8:257170/1‑149 (MQ=255)
  cgcTATTCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCgctgc                                                                                                                                                >  5:227906/1‑149 (MQ=255)
    cTATTCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGC‑GTGAAA‑‑CCGAGCAGCGCCAGGCGCCGCTc                                                                                                                                              <  6:318425/149‑1 (MQ=255)
    cTATTCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGC‑GTGAAA‑‑CCGAGCAGCGCCAGGCGCCGCTc                                                                                                                                              <  8:104297/149‑1 (MQ=255)
      aTTCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCgg                                                                                                                                            >  1:192046/1‑149 (MQ=255)
       ttCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCg                                                                                                                                             <  6:301635/147‑1 (MQ=255)
       ttCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGAAACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCgg                                                                                                                                            >  5:233098/1‑148 (MQ=255)
          aGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCAGAAACC‑‑CCGAGCAGCGCCAGGCGCCGCTCggttt                                                                                                                                         >  2:164584/1‑146 (MQ=38)
          aGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTc                                                                                                                                              <  5:57749/143‑1 (MQ=255)
          aGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTc                                                                                                                                              >  6:57749/1‑143 (MQ=255)
            gACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCGGGGTTTc                                                                                                                                      >  1:182737/1‑149 (MQ=255)
            gACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGACGATTGCGGCTTTTCCCTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCGGGGTTTc                                                                                                                                      >  6:112876/1‑149 (MQ=255)
              ccaccTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTg                                                                                                                                    <  7:257170/149‑1 (MQ=255)
               caccTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGC‑GTGAAACCCCGAGCAGCGCCAGGCGCCGCTCggtttcacg                                                                                                                                     <  8:103522/149‑8 (MQ=38)
               caccTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTg                                                                                                                                    >  1:180761/1‑148 (MQ=255)
                accTGGGCTCAGTGCGCTTCGCGGTACGCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGC‑GTGAAA‑‑CCGCGCGGCGCCTGGCGCTGCTCGGGGTTTCTGca                                                                                                                                  <  8:263877/149‑1 (MQ=255)
                 ccTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGcag                                                                                                                                 <  6:246570/149‑1 (MQ=255)
                     ggCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGcagctc                                                                                                                              <  5:112876/148‑1 (MQ=255)
                            tGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTGTCCCTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTcagt                                                                                                                      <  7:314330/149‑1 (MQ=255)
                                     cgGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATGGCCAGTGGTGACAGGTTCATCCTGGTCGATTGCGGCTTTTCCCTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTg                                                                                                             <  6:227906/149‑1 (MQ=255)
                                     cgGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTg                                                                                                             <  3:120490/149‑1 (MQ=255)
                                      ggTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCGGGGttt                                                                                                                                       <  3:209948/122‑1 (MQ=255)
                                      ggTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCGGGGttt                                                                                                                                       >  4:209948/1‑122 (MQ=255)
                                          cTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCgg                                                                                                                                            <  4:235746/113‑1 (MQ=255)
                                          cTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCgg                                                                                                                                            >  3:235746/1‑113 (MQ=255)
                                                        ccAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGcc                                                                                           >  2:214686/1‑148 (MQ=255)
                                                        ccAACGGAACGGCACGCACATCGCCACAGCTCACACGTTCATCCTGGTCCATTGCGGCTTTTCCCTGC‑GTCAAA‑‑CCGAGCGGCGCCTGCCGCTGCTCCTGGTTTCTGCACCTCAGCTCAGTGCAGTCCTGTTCACCAACGAACAtcac                                                                                           >  4:76831/1‑145 (MQ=255)
                                                         cAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGa                                                                                         >  6:216309/1‑149 (MQ=255)
                                                           aGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGAcc                                                                                       <  3:76831/149‑1 (MQ=255)
                                                           aGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGAcc                                                                                       <  1:214686/149‑1 (MQ=255)
                                                              gAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTg                                                                                                                                    <  1:82222/101‑1 (MQ=255)
                                                              gAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTg                                                                                                                                    >  2:82222/1‑101 (MQ=255)
                                                               aaCGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGt                                                                                   <  2:182737/149‑1 (MQ=255)
                                                                    cACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATg                                                                              <  2:180761/149‑1 (MQ=255)
                                                                    cACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATg                                                                              <  5:216309/149‑1 (MQ=255)
                                                                    cACGCTGATCGCCAGTGGTGACACGTTCATCCTGGGCGATTGCGGCTTTTCCCTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCAt                                                                               <  1:332404/148‑1 (MQ=255)
                                                                     aCGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCg                                                                                          <  4:319278/136‑1 (MQ=255)
                                                                     aCGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCg                                                                                          >  3:319278/1‑136 (MQ=255)
                                                                                      tgACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCAc                                                            >  4:224208/1‑149 (MQ=255)
                                                                                      tgACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCAc                                                            <  3:224208/149‑1 (MQ=255)
                                                                                        acacGTTCATCCTGGTCGATTGCGGCTTTTCCCTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACgg                                                          >  2:150964/1‑149 (MQ=255)
                                                                                          acGTTCATCCTGGTCGATTGCGGCTTTTCCCTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTcagtg                                                                                                                     >  4:8912/1‑88 (MQ=255)
                                                                                                   cTGGTCGATTGCGGCTTTTCCCTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACAAGCCGACCCCATGCACGTGGTCGGcat                                                                    <  4:237609/128‑3 (MQ=255)
                                                                                                              cGGCTTTTCCCTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTAcc                                    >  7:277126/1‑149 (MQ=255)
                                                                                                                 cTTTTCCCTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCt                                   >  7:149304/1‑147 (MQ=255)
                                                                                                                       ccTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTAc                                             >  6:43136/1‑131 (MQ=255)
                                                                                                                       ccTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTAc                                             <  5:43136/131‑1 (MQ=255)
                                                                                                                        cTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAgg                          >  7:266576/1‑149 (MQ=255)
                                                                                                                             gTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGgcgc                                                       <  4:272394/116‑1 (MQ=255)
                                                                                                                             gTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGgcgc                                                       >  3:272394/1‑116 (MQ=255)
                                                                                                                                  accccGAGCAGCGCCAGGCGCCGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTg                   >  4:221568/4‑149 (MQ=255)
                                                                                                                                  a‑‑CCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTgcg                 <  1:150964/149‑1 (MQ=255)
                                                                                                                                       gAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTgcgcat              >  7:236186/1‑147 (MQ=255)
                                                                                                                                          cggcgCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGTGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCAt           <  8:266576/149‑1 (MQ=255)
                                                                                                                                                   gcgcTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGTGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGcc  >  2:268426/1‑149 (MQ=255)
                                                                                                                                                       |                                                                                                                                                
AGCGCTATTCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGC‑GTGAAA‑‑CCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCC  >  NC_002947/1416726‑1417018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑