Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	184,287	A→C	5.3%	H92Q (CAT→CAG)	PP_0160 ←	ferrioxamine receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	184,287	0	A	C	5.3%	237.4 / 3.6	76	H92Q (CAT→CAG)	PP_0160	ferrioxamine receptor
Reads supporting (aligned to +/- strand):  ref base A (34/38);  new base C (2/2);  total (36/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

TGTCTGGCAATGCCAGGGCGGCGTTCTGACGCTGTGCCTGCA‑GCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTATTGCATAGTGTTGGCTCACTTCGGCTGTGTCGGCCGCAACGGAAAGTTGC  >  NC_002947/184142‑184431                                                                                                                                                   |                                                                                                                                                  gcccTGGCATTGCCAGGGCGGCGTTCTGACGCTGTGCCTGCA‑GCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATg                                                                                                                                                 >  1:346677/4‑148 (MQ=255)   tCTGGCAATGCCAGGGCGGCGTTCTGACGCTGTGCCTGCA‑GCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCa                                                                                                                                               <  5:274750/148‑1 (MQ=255)   tCTGGCAATGCCAGGGCGGCGTTCTGACGCTGTGCCTGCA‑GCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAg                                                                                                                                              <  6:337518/149‑1 (MQ=255)     tGGCAATGCCAGGGCGGCGTTCTGACGCTGTGCCTGCA‑GCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCATTCCAACGGACTGCAg                                                                                                                                              >  5:307708/1‑147 (MQ=255)     tGGCAATGCCAGGGCGGCGTTCTGACGCTGTGCCTGCA‑GCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCATTCCAACGGACTGCAg                                                                                                                                              <  6:307708/147‑1 (MQ=255)     tGGCAATGCCAGGGCGGCGTTCTGACGCTGTGCCTGCA‑GCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGc                                                                                                                                            >  5:60126/1‑149 (MQ=255)     tGGCAATGCCAGGGCGGCGTTCTGACGCTGTGCCTGCA‑GCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGc                                                                                                                                            >  4:301641/1‑149 (MQ=255)       gCAATGCCAGGGCGGCGTTCTGACGCTGTGCCTGCA‑GCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCa                                                                                                                                          >  1:7071/1‑149 (MQ=255)          aTGCCAGGGCGGCGTTCTGACGCTGTGCCTGCA‑GCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCg                                                                                                                                       <  6:116790/149‑1 (MQ=255)             ccAGGGCGGCGTTCTGACGCTGTGCCTGCA‑GCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAg                                                                                                                                              <  8:258996/139‑1 (MQ=255)             ccAGGGCGGCGTTCTGACGCTGTGCCTGCA‑GCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAg                                                                                                                                              >  7:258996/1‑139 (MQ=255)              cAGGGCGGCGTTCTGACGCTGTGCCTGCA‑GCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCa                                                                                                                                               >  5:181540/1‑137 (MQ=255)              cAGGGCGGCGTTCTGACGCTGTGCCTGCA‑GCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCa                                                                                                                                               <  6:181540/137‑1 (MQ=255)               aGGGCGGCGTTCTGACGCTGTGCCTGCA‑GCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAAt                                                                                                                                                  <  1:198364/133‑1 (MQ=255)               aGGGCGGCGTTCTGACGCTGTGCCTGCA‑GCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAAt                                                                                                                                                  >  2:198364/1‑133 (MQ=255)               aGGGCGGCGTTCTGACGCTGTGCCTGCA‑GCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGc                                                                                                                                                <  5:3355/135‑1 (MQ=255)               aGGGCGGCGTTCTGACGCTGTGCCTGCA‑GCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGc                                                                                                                                                >  6:3355/1‑135 (MQ=255)                gggCGGCGTTCTGACGCTGTGCCTGCA‑GCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGc                                                                                                                                <  5:181320/149‑1 (MQ=255)                 ggcggcGTTCTGACGCTGTGCCTGCA‑GCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCt                                                                                                                               <  3:301641/149‑1 (MQ=255)                       gTTCTGACGCTGTGCCTGCA‑GCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCg                                                                                                                         >  3:236217/1‑149 (MQ=255)                        ttCTGACGCTGTGCCTGCA‑GCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGt                                                                                                                        >  3:12792/1‑149 (MQ=255)                           tGACGCTGTGCCTGCA‑GCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCt                                                                                                                     >  6:122010/1‑149 (MQ=255)                            gACGCTGTGCCTGCA‑GCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGc                                                                                                                      <  6:145347/147‑1 (MQ=255)                            gACGCTGTGCCTGCA‑GCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGc                                                                                                                      >  5:145347/1‑147 (MQ=255)                               gCTGTGCCTGCA‑GCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGa                                                                                                                  >  2:227010/1‑148 (MQ=255)                               ctatgtgcTGCAGGCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGa                                                                                                                  <  8:19824/142‑1 (MQ=255)                                tatgtgcTGCAGGCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGa                                                                                                                  >  6:110167/7‑148 (MQ=255)                                   tgCCTGCA‑GCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAggg                                                                                                             <  1:95622/149‑1 (MQ=255)                                   tgCCTGCA‑GCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAgg                                                                                                              <  2:346677/148‑1 (MQ=255)                                            gcaCATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGTGATCCCg                                                                                                     <  5:165559/149‑1 (MQ=255)                                                aTAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCt                                                                                                 <  4:12792/149‑1 (MQ=255)                                                      aCGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGgcga                                                                                            <  5:110167/148‑1 (MQ=255)                                                        gTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGgcgagc                                                                                          >  7:310494/1‑148 (MQ=255)                                                         tCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGTGATCCCAGCCTGgcgagcga                                                                                        >  5:130827/1‑149 (MQ=255)                                                           cccccTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCTCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGAtt                                                                                      <  1:227010/149‑1 (MQ=255)                                                           cccccTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGAtt                                                                                      <  8:310494/149‑1 (MQ=255)                                                              ccTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGAtt                                                                                      >  3:290640/1‑146 (MQ=255)                                                              ccTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGAtt                                                                                      <  4:290640/146‑1 (MQ=255)                                                                    tGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTg                                                                                                <  4:280297/130‑1 (MQ=255)                                                                    tGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTg                                                                                                >  3:280297/1‑130 (MQ=255)                                                                    tGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGc                                                                             <  2:7071/149‑1 (MQ=255)                                                                      aCGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCa                                                                            <  6:60126/148‑1 (MQ=255)                                                                        ggCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑Ca                                                                                                                                  >  8:268165/1‑92 (MQ=255)                                                                        ggCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑Ca                                                                                                                                  <  7:268165/92‑1 (MQ=255)                                                                        ggCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGt                                                                         >  6:348991/1‑149 (MQ=255)                                                                           ttCCAGTCTGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAg                                                                                                               <  1:309835/108‑1 (MQ=255)                                                                           ttCCAGTCTGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAg                                                                                                               >  2:309835/1‑108 (MQ=255)                                                                           ttCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCgg                                                                      >  8:332153/1‑149 (MQ=255)                                                                                 tCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGtt                                                                <  7:332153/149‑1 (MQ=255)                                                                                    gCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGc                                                             >  4:81581/1‑149 (MQ=255)                                                                                      tGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAggg                                                                                                             <  6:165874/99‑1 (MQ=255)                                                                                      tGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAggg                                                                                                             >  5:165874/1‑99 (MQ=255)                                                                                        ccATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTgtgt                                                                                  >  8:183986/1‑124 (MQ=255)                                                                                        ccATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTgtgt                                                                                  <  7:183986/124‑1 (MQ=255)                                                                                         cATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCt                                                        <  5:122010/149‑1 (MQ=255)                                                                                                 aaCTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGGTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTATTGc                                                <  4:314579/149‑1 (MQ=255)                                                                                                      gcgcAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTATTGCATa                                             <  5:348991/147‑1 (MQ=255)                                                                                                      gcgcAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTATTGCATa                                             <  4:236217/147‑1 (MQ=255)                                                                                                           gcgcCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTATTGCATAGTGTTgg                                      <  3:81581/149‑1 (MQ=255)                                                                                                                  ttCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGGGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTATTGCATAGTGTTGGCt                                    <  4:162040/143‑1 (MQ=255)                                                                                                                  aTCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTATTGCATAGTGTTGGCt                                    >  3:162040/1‑144 (MQ=255)                                                                                                                      gCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTATTGCATAGTGTTGGCTCACTTCGGc                           >  5:110895/1‑149 (MQ=255)                                                                                                                        tgtgTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGTGCAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTATTGCATAGTGTTgg                                      >  1:242774/1‑137 (MQ=255)                                                                                                                        tgtgTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTATTGCATAGTGTTgg                                      <  2:242774/136‑1 (MQ=255)                                                                                                                          tgtACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTATTGCATAGTGTTGGCTCACTTCGGCtgtg                       >  2:16113/1‑149 (MQ=255)                                                                                                                            tACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTATTGCATAGTGTTgg                                      <  3:184451/132‑1 (MQ=255)                                                                                                                            tACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTATTGCATAGTGTTgg                                      >  4:184451/1‑132 (MQ=255)                                                                                                                            tACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTATTGCATAGTGTTGGCTCACTTCgg                            <  3:247712/142‑1 (MQ=255)                                                                                                                            tACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTATTGCATAGTGTTGGCTCACTTCgg                            >  4:247712/1‑142 (MQ=255)                                                                                                                              cTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTATTGCATAGTGTTGGCTCACTTCGGCTGTGTCgg                   >  3:95207/1‑149 (MQ=255)                                                                                                                                   ccTGCATTCCAACGGACTGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTATTGCATAGTGTTGGCTCACTTCGGCTGTGTCGGCCGc               >  8:206768/1‑148 (MQ=255)                                                                                                                                         ttccaacggACTGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTATTGCATAGTGTTGGCTCACTTCGGCTGTGTCGGCCGCAACGGaa        <  7:206768/142‑1 (MQ=255)                                                                                                                                            cGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTATTGCATAGTGTTGGCTCACTTCGGCTGTGTCGGCCGCAACGGAAAGt     >  3:325113/1‑149 (MQ=255)                                                                                                                                             gTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTATTGCATAGTGTTGGCTCACTTCGGCTGTGTCGGCCGCAACGGAAAGtt    >  6:284588/1‑149 (MQ=255)                                                                                                                                                ggAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTATTGCATAGTGTTGGCTCACTTCGGCTGTGTCGGCCGCAACGGAAAGTTGc  <  4:95207/148‑1 (MQ=255)                                                                                                                                                ggAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTATTGCATAGTGTTGGCTCACTTCGGCTGTGTCGGCCGCAACGGAAAGTTGc  <  1:16113/148‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  TGTCTGGCAATGCCAGGGCGGCGTTCTGACGCTGTGCCTGCA‑GCACATAGTTACGTCCCCCCTGGCTGACGGCTTCCAGTCCGCTGCCATTCAGCAACTGGCGCAGCGCCTGATCGGCTGTGTACTGCCCCTGCAGTCCGTTGGAATGCAGGCCATCGGT‑CAGCTGAGGCGTGCTCGATAGGGTGATCCCGGCCTGGCGAGCGATTGTGTTGAGCACGTCGGTCAGTTGGCCAGCTGGTATTGCATAGTGTTGGCTCACTTCGGCTGTGTCGGCCGCAACGGAAAGTTGC  >  NC_002947/184142‑184431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑