Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,439,968 A→G 9.7% intergenic (‑299/‑360) yveA ← / → PP_1260 aspartate‑proton symporter/transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,439,9680AG 9.7% 109.2 / 2.1 41intergenic (‑299/‑360)yveA/PP_1260aspartate‑proton symporter/transposase
Reads supporting (aligned to +/- strand):  ref base A (17/20);  new base G (2/2);  total (19/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACGAAATCGGCACAGTTGCAGAAAAACAGTGGTAAGCCGCAAAGCGTGGGGATAGGCTCAAAGCCATACCGGATAAGGGTTTGAGAGATGCAGTGCTAGTGCCGGCCCTTTCGCGGGTG‑AACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCC‑TGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGC  >  NC_002947/1439824‑1440102
                                                                                                                                                 |                                                                                                                                       
gACGAAATCGGCACAGTTGCAGAAAAACAGTGGTAAGCCGCAAAGCGTGGGGATAGGCTCAAAGCCATACCGGATAAGGGTTTGAGAGATGCAGTGCTAGTGCCGGCCCTTTCGCGGGTG‑AACCCGCTCCTACAGGTACGGCGCGGAAt                                                                                                                                     >  2:261353/1‑149 (MQ=255)
gACGAAATCGGCACAGTTGCAGAAAAACAGTGGTAAGCCGCAAAGCGTGGGGATAGGCTCAAAGCCATACCGGATAAGGGTTTGAGAGATGCAGTGCTAGTGCCGGCCCTTTCGCGGGTG‑AACCCGCTCCGACAGGTACGGCGCGGAAt                                                                                                                                     >  2:265734/1‑149 (MQ=255)
          gCACAGTTGCAGAAAAACAGTGGTAAGCCGCAAAGCGTGGGGATAGGCTCAAAGCCATACCGGATAAGGGTTTGAGAGATGCAGTGCTAGTGCCGGCCCTTTCGCGGGTG‑ACCCCGCTCCTACAGGTCCGGCGCAGAATTCGAACTCtg                                                                                                                           >  6:185981/1‑149 (MQ=255)
          gCACAGTTGCAGAAAAACAGTGGTAAGCCGCAAAGCGTGGGGATAGGCTCAAAGCCATACCGGATAAGGGTTTGAGAGATGCAGTGCTAGTGCCGGCCCTTTCGCGGGTG‑AACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCtg                                                                                                                           >  1:275437/1‑149 (MQ=255)
            acaGTTGCAGAAAAACAGTGGTAAGCCGCAAAGCGTGGGGATAGGCTCAAAGCCATACCGGATAAGGGTTTGAGAGATGCAGTGCTAGTGCCGGCCCTTTCGCGGGTG‑AACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCtgtg                                                                                                                         <  4:7703/149‑1 (MQ=255)
            acaGTTGCAGAAAAACAGTGGTAAGCCGCAAAGCGTGGGGATAGGCTCAAAGCCATACCGGATAAGGGTTTGAGAGATGCAGTGCTAGTGCCGGCCCTTTCGCGGGTG‑AACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCtgtg                                                                                                                         <  8:246162/149‑1 (MQ=255)
             caGTTGCAGAAAAACAGTGGTAAGCCGCAAAGCGTGGGGATAGGCTCAAAGCCATACCGGATAAGGGTTTGAGAGATGCAGTGCTAGTGCCGGCCCTTTCGCGGGTG‑AACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTgg                                                                                                                        <  8:212170/149‑1 (MQ=255)
                   cAGAAAAACAGTGGTAAGCCGCAAAGCGTGGGGATAGGCTCAAAGCCATACCGGATAAGGGTTTGAGAGATGCAGTGCTAGTGCCGGCCCTTTCGCGGGTG‑AACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGcc                                                                                                                   <  2:62669/148‑1 (MQ=255)
                         aaCAGTGGTAAGCCGCAAAGCGTGGGGATAGGCTCAAAGCCATACCGGATAAGGGTTTGAGAGATGCAGTGCTAGTGCCGGCCCTTTCGCGGGTG‑AACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCC‑TGTAgg                                                                                                           <  2:275437/149‑1 (MQ=255)
                               ggTAAGCCGCAAAGCGTGGGGATAGGCTCAAAGCCATACCGGATAAGGGTTTGAGAGATGCAGTGCTAGTGCCGGCCCTTTCGCGGGTG‑AACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCC‑TGTAGGAGATCa                                                                                                     <  7:248640/149‑1 (MQ=255)
                                gTAAGCCGCAAAGCGTGGGGATAGGCTCAAAGCCATACCGGATAAGGGTTTGAGAGATGCAGTGCTAGTGCCGGCCCTTTCGCGGGTG‑AACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCC‑TGTAGGAGATCAc                                                                                                    >  5:211031/1‑149 (MQ=255)
                                   aGCCGCAAAGCGTGGGGATAGGCTCAAAGCCATACCGGATAAGGGTTTGAGAGATGCAGTGCTAGTGCCGGCCCTTTCGCGGGTG‑AACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCC‑TGTAGGAGATCAcc                                                                                                   >  3:290127/1‑147 (MQ=255)
                                           aGCGTGGGGATAGGCTCAAAGCCATACCGGATAAGGGTTTGAGAGATGCAGTGCTAGTGCCGGCCCTTTCGCGGGTG‑AACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCC‑TGTAGGAGATCACCCAGCCCTTcc                                                                                         <  6:211031/149‑1 (MQ=255)
                                                        gCTCAAAGCCATACCGGTTAAGGGTTTGAGAGATGCAGTGCTAGTGCCGGCCCTTTCGCGTGTG‑AACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCC‑TGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTc                                                                            <  8:225812/149‑1 (MQ=255)
                                                            aaaGCCATACCGGATAAGTGTTTGAGAGATACAGTGCTAGTGCCGGCCCTTTCGCGGGTG‑AACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCC‑TGTAGga                                                                                                          <  5:284713/115‑1 (MQ=255)
                                                            aaaGCCATACCGGATAAGGGTTTGAGAGATACAGTGCTAGTGCAGGCCCTTTCGCGGGTG‑AACCCGATCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGCGGCCC‑GGTAGga                                                                                                          >  6:284713/1‑115 (MQ=39)
                                                                  aTACCGGATAAGGGTTTGAGAGATGCAGTGCTAGTGCCGGCCCTTTCGCGGGTG‑AACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCC‑TGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCaaa                                                                  <  7:3018/149‑1 (MQ=255)
                                                                        gctAACGGTTTGACAGATGCACGGCTATGGCCCGGCCATTCCCGGGGG‑AACCCGCCCCTACCCGGACCGCGCGGAAATCGAAAACCGTGGGGGCCCTTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGa                                                             <  1:261353/147‑1 (MQ=12)
                                                                        gATAAGGGTTTGAGAGATGCAGTGCTAGTGCCGGCCCTTTCGCGGGTG‑AACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCC‑TGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAg                                                            <  4:290127/149‑1 (MQ=255)
                                                                        gATAAGGGTTTGAGACAAGCCCGGCTACGGCCGGGCCCTTCGCGGGTGTAACCCGC‑CCTACAGGTACCGCGCGGTAATCGAAATCTGGGGGGGCCCTTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGa                                                             <  1:265734/149‑1 (MQ=16)
                                                                            aGGGTTTGAGAGATGCAGTGCTAGTGCCGGCCCTTTCGCGGGTG‑AACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCC‑TGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGgtg                                                         >  8:88707/1‑148 (MQ=255)
                                                                                     gagaTGCAGTGCTAGTGCCGGCCCTTTCGCGGGTG‑AACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCC‑TGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCa                                               <  7:88707/149‑1 (MQ=255)
                                                                                      agaTGCAGTGCTAGTGCCGGCCCTTTCGCGGGTG‑AACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCC‑TGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCa                                               <  3:205994/148‑1 (MQ=255)
                                                                                            aGTGCTAGTGCCGGCCCTTTCGCGGGTG‑AACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCC‑TGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCg                                         >  1:45797/1‑148 (MQ=255)
                                                                                              tGCTAGTGCCGGCCCTTTCGCGGGTG‑AACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCC‑TGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAg                                                            <  4:333070/127‑1 (MQ=255)
                                                                                              tGCTAGTGCCGGCCCTTTCGCGGGTG‑AACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCC‑TGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAg                                                            >  3:173523/1‑127 (MQ=255)
                                                                                              tGCTAGTGCCGGCCCTTTCGCGGGTG‑AACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCC‑TGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAg                                                            >  3:333070/1‑127 (MQ=255)
                                                                                              tGCTAGTGCCGGCCCTTTCGCGGGTG‑AACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCC‑TGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAg                                                            <  4:173523/127‑1 (MQ=255)
                                                                                                             tttCGCGGGTG‑AACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCC‑TGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACAtttt                       >  6:13908/1‑149 (MQ=255)
                                                                                                                           cccGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCC‑TGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGaaaaa                 >  3:339429/1‑142 (MQ=255)
                                                                                                                           cccGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCC‑TGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGaaaaa                 <  4:339429/142‑1 (MQ=255)
                                                                                                                           cccGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCC‑TGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAgg          >  7:256872/1‑149 (MQ=255)
                                                                                                                              gCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCC‑TGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCgg       >  3:234140/1‑149 (MQ=255)
                                                                                                                              gCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCC‑TGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCgg       <  2:45797/149‑1 (MQ=255)
                                                                                                                                   tACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCC‑TGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTcc                                          <  4:125364/109‑1 (MQ=255)
                                                                                                                                   tACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCC‑TGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTcc                                          >  3:125364/1‑109 (MQ=255)
                                                                                                                                   tACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCC‑TGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGc  >  1:25966/1‑149 (MQ=255)
                                                                                                                                    aCAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCC‑TGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCt                               >  7:174208/1‑119 (MQ=255)
                                                                                                                                    aCAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCC‑TGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCt                               <  8:174208/119‑1 (MQ=255)
                                                                                                                                      aGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCC‑TGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTg    <  7:19236/144‑1 (MQ=255)
                                                                                                                                      aGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCC‑TGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTg    >  8:19236/1‑144 (MQ=255)
                                                                                                                                                 |                                                                                                                                       
GACGAAATCGGCACAGTTGCAGAAAAACAGTGGTAAGCCGCAAAGCGTGGGGATAGGCTCAAAGCCATACCGGATAAGGGTTTGAGAGATGCAGTGCTAGTGCCGGCCCTTTCGCGGGTG‑AACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCC‑TGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGC  >  NC_002947/1439824‑1440102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑