Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,495,654 T→G 13.0% L28R (CTG→CGG) ‡ mdeA → methionine gamma‑lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,495,6540TG13.0% 132.5 / 13.9 54L28R (CTG→CGG) ‡mdeAmethionine gamma‑lyase
Reads supporting (aligned to +/- strand):  ref base T (23/23);  new base G (2/5);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.72e-01

GAAAAAATCGGGCAGCTCGGTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCTGGAGCAGCGCATGGCTTC  >  NC_002947/1495514‑1495798
                                                                                                                                            |                                                                                                                                                
gAAAAAATCGGGCAGCTCGGTGGCATAAGCTTATATCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCAc                                                                                                                                          <  2:262732/149‑1 (MQ=255)
 aaaaaaTCGGGCAGCTCGGTGGCATAAGCTTATTGCAAGCCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTTGTCCACGCGGGCCATTCATCATGGCTATGAGCCTCTTTCCCATGGTGGAGCCCTGGTGccc                                                                                                                                           >  6:72988/1‑146 (MQ=255)
   aaaaTCGGGCAGCTCGGTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCACCAGGGCGCCAccat                                                                                                                                       <  6:25933/149‑3 (MQ=255)
    aaaTCGGGCAGCTCGGTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCACCAGGGCGCCAccatg                                                                                                                                      <  8:119148/149‑4 (MQ=255)
     aaTCGGGCAGCTCGGTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGTGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGgtg                                                                                                                                     >  2:133001/1‑149 (MQ=255)
      aTCGGGCAGCTCGGTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCACCAGGGCGCCAccat                                                                                                                                       >  8:195945/1‑144 (MQ=255)
      aTCGGGCAGCTCGGTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCACCAGGGCGCCAccat                                                                                                                                       <  7:195945/146‑3 (MQ=255)
                  ggTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGc                                                                                                                          <  1:25471/147‑1 (MQ=255)
                    tGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTAcc                                                                                                                      >  1:95275/1‑149 (MQ=255)
                        aTAAGCTTATAGCAAACCACAAGAGGCTGATGCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTACCATGGTGGCGCCCAGGTGCCACCGGTGTACCAGACGGCTACCTATg                                                                                                                  >  1:201829/1‑149 (MQ=255)
                         tAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTAc                                                                                                                                  <  8:168153/132‑1 (MQ=255)
                         tAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTAc                                                                                                                                  >  7:168153/1‑132 (MQ=255)
                                   gCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACt                                                                                                       <  4:276493/149‑1 (MQ=255)
                                   gCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACt                                                                                                       <  4:15614/149‑1 (MQ=255)
                                         cacaAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAg                                                                                                 >  3:20400/1‑149 (MQ=255)
                                         cacaAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCACCAGGGCGCCAcc                                                                                                                                         >  5:321929/1‑109 (MQ=255)
                                         cacaAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCACCAGGGCGCCAcc                                                                                                                                         <  6:321929/109‑1 (MQ=255)
                                           caAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTa                                                                                               >  4:281424/1‑149 (MQ=255)
                                               agGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGt                                                                                           <  2:95275/149‑1 (MQ=255)
                                                    gTTGCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGc                                                                                                                 <  1:143556/122‑1 (MQ=255)
                                                    gTTGCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGc                                                                                                                 >  2:143556/1‑122 (MQ=255)
                                                    gTTGCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGa                                                                                                  <  5:51757/137‑1 (MQ=255)
                                                    gTTGCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCACCACTGTCGa                                                                                                  >  6:51757/1‑137 (MQ=255)
                                                     ttGCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGtgctgc                                                                                      <  3:204809/148‑1 (MQ=255)
                                                       gCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGctgcctg                                                                                   >  2:320787/1‑149 (MQ=255)
                                                              gcgACTCCCACCCCAACACCGGTTTTGCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGcc                                                                            >  1:186367/1‑149 (MQ=255)
                                                                 aCTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCggg                                                                         <  3:281424/149‑1 (MQ=255)
                                                                   tCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCgg                                                                          >  5:100524/1‑146 (MQ=255)
                                                                   tCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCgg                                                                          <  6:100524/146‑1 (MQ=255)
                                                                           caacaCCGGTTTTTCCACGTGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTg                                                               <  1:133001/149‑1 (MQ=255)
                                                                             acacCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTggg                                                             <  2:201829/149‑1 (MQ=255)
                                                                                   gTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGa                                                                                                  >  6:100027/1‑106 (MQ=255)
                                                                                   gTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTAc                                                                                              <  7:271180/110‑1 (MQ=255)
                                                                                   gTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTAc                                                                                              >  8:271180/1‑110 (MQ=255)
                                                                                       ttCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTa                                                                                               <  6:155634/105‑1 (MQ=255)
                                                                                       ttCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTa                                                                                               >  5:155634/1‑105 (MQ=255)
                                                                                           aCGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCg                                               >  6:349600/1‑149 (MQ=255)
                                                                                                           atGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACa                                                   >  8:232631/1‑129 (MQ=255)
                                                                                                           atGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACa                                                   <  7:232631/129‑1 (MQ=255)
                                                                                                           atGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACg                               <  4:20400/149‑1 (MQ=255)
                                                                                                              gCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTg                            >  4:42238/1‑149 (MQ=255)
                                                                                                                  tGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGcc                         <  5:349600/148‑1 (MQ=255)
                                                                                                                    aCCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTg                      >  1:226372/1‑149 (MQ=255)
                                                                                                                        gCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGgga                                                                       >  6:160770/1‑96 (MQ=255)
                                                                                                                             cgcATGGTGGCGCCCTGGTGCGACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCTGGagca              <  2:186367/146‑1 (MQ=255)
                                                                                                                              ccATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTcc                                        >  2:271071/1‑121 (MQ=255)
                                                                                                                              ccATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTcc                                        <  1:271071/121‑1 (MQ=255)
                                                                                                                              ccATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCTGGagcagc            >  7:213482/1‑149 (MQ=255)
                                                                                                                              ccATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCTGGagcagc            >  1:319245/1‑149 (MQ=255)
                                                                                                                              caccggtggCACCAGGGCGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGgga                                                                       <  1:331843/86‑1 (MQ=38)
                                                                                                                                 tggtggCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCTGGAGCAGCGCa         <  8:213482/149‑1 (MQ=255)
                                                                                                                                        gcCCTGGTGCCACCTGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCGGCCTGCTTCGCCGGGGACGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCTGGAGCAGCGCATGGCTTc  <  5:72988/149‑1 (MQ=255)
                                                                                                                                        gcCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCTgg                  <  6:50720/133‑1 (MQ=255)
                                                                                                                                        gcCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCTgg                  >  5:50720/1‑133 (MQ=255)
                                                                                                                                            |                                                                                                                                                
GAAAAAATCGGGCAGCTCGGTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTTTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCTGGAGCAGCGCATGGCTTC  >  NC_002947/1495514‑1495798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑