| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,504,067 | T→A | 20.1% | intergenic (+34/‑229) | rpsI → / → petA | 30S ribosomal protein S9/ubiquinol‑cytochrome c reductase iron‑sulfur subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,504,067 | 0 | T | A | 20.1% | 105.0 / 19.6 | 54 | intergenic (+34/‑229) | rpsI/petA | 30S ribosomal protein S9/ubiquinol‑cytochrome c reductase iron‑sulfur subunit |
| Reads supporting (aligned to +/- strand): ref base T (19/24); new base A (6/5); total (25/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCG‑TAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCTTGTCAGTGTGTGGATTTGTTTCTTACCATGAC > NC_002947/1503928‑1504215 | cgcTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCTTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGGG‑TAAGCGTCCTCAGTACTCCAAGCGTTACTACCGCTTCGGCATTCGAAAAAAGCCCGGTTCCAAGGAACCgg < 5:116681/149‑1 (MQ=255) gcTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCG‑TAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCggg < 8:157001/149‑1 (MQ=255) cgtcgtGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCG‑TAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑tt < 7:314030/146‑1 (MQ=255) tcgtGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCG‑TAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑ttttttt > 7:267430/1‑149 (MQ=255) tGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGAAAACCTTCGGAATTATACAAACCGTTAATACAGGTTCGGCATACAAAAAAACCCCGGTTCGTTGGAACCGGGG‑ttttttttt > 1:104054/1‑149 (MQ=255) tGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCG‑TAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCt < 6:230245/128‑1 (MQ=255) tGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCG‑TAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCAAGGAACCGGGT‑ttttttcgac < 2:108558/149‑5 (MQ=255) gCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCG‑TAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCgg < 4:39497/132‑1 (MQ=255) gCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCG‑TAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCgg > 3:39497/1‑132 (MQ=255) cTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCG‑TAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTg < 1:91029/149‑1 (MQ=255) cGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCG‑TAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGaa < 1:201224/148‑1 (MQ=255) aCCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCG‑TAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAAc < 2:21496/149‑1 (MQ=255) ccGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCG‑TAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCt > 2:196873/1‑149 (MQ=255) tCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCG‑TAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTcagcag > 2:22283/1‑148 (MQ=255) ttaGCGTAAGAAAGTTGGTCTGCGTAAAGCGCT‑TAAGCGTCCTCAGTACTCCAAGCTTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGcc < 2:104054/146‑1 (MQ=255) cGTAAGAAAGTGGGTCTGCGTAAAGCGCG‑TAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCt > 2:45745/1‑127 (MQ=255) cGTAAGAAAGTGGGTCTGCGTAAAGCGCG‑TAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCt < 1:45745/127‑1 (MQ=255) gTAAGAAAGTGGGTCTGCGTAAAGCGCG‑TAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGt < 1:22283/149‑1 (MQ=255) aaGTGGGTCTGCGTAAAGCGCG‑TAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCAt < 2:314553/148‑1 (MQ=255) aaGTGGGTCTGCGTAAAGCGCG‑TAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCAt < 4:80174/148‑1 (MQ=255) aaGTGGGTCTGCGTAAAGCGCG‑TAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTCTCAGCAGTCAGTGGCCGCTTGTCGCATa < 1:196873/149‑1 (MQ=255) aGTGGGTCTGCGTAAAGCGCG‑TAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCAt < 8:267430/147‑1 (MQ=255) tGCGTAAAGCGCG‑TAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGcc < 3:287631/99‑1 (MQ=255) tGCGTAAAGCGCG‑TAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGcc > 4:287631/1‑99 (MQ=255) gCGTAAATCCCG‑TAATCGTCCTCAGTACTCCAAGCGTTAATACCCCTTCGGCACTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGAt < 6:181563/149‑1 (MQ=255) aGCGCG‑TAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCa > 8:85454/1‑148 (MQ=255) gcgcG‑TAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGc > 8:281240/1‑78 (MQ=255) gcgcG‑TAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGc < 7:281240/78‑1 (MQ=255) gcg‑TAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGc < 7:38224/149‑1 (MQ=255) cg‑TAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGAc < 8:130862/132‑1 (MQ=255) cg‑TAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGAc > 7:130862/1‑132 (MQ=255) tccgatcTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTg < 7:235153/141‑1 (MQ=255) aGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTgg < 7:85454/148‑1 (MQ=255) aGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTg > 8:235153/1‑138 (MQ=255) aGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGccc > 2:171333/1‑149 (MQ=255) tCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCa < 1:135955/109‑1 (MQ=255) tCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCa > 2:135955/1‑109 (MQ=255) cAAGCGTTAATACCGCTTTGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTATTTTTAGCCTTGAAATAACCTGTCAGCAGACAGTCGCCGCTTGTCGCATAGAAGAAGATCAAGCAAAGCCTGGGTAGTCGCCCTTGCCgg > 2:144684/1‑149 (MQ=255) cAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTaa < 8:122108/77‑1 (MQ=255) cAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCgg > 7:174372/1‑149 (MQ=255) aaGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTc > 3:86008/1‑137 (MQ=255) aaGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTc < 4:86008/137‑1 (MQ=255) aTACCGCTTCGGCAGTCGAAAAAAGCCCGGTTACTTAGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTggg > 7:242725/1‑124 (MQ=255) ctaaaaaaaaaGCCCGGTTCCAAGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCTTGTCAgtgtgt > 7:61354/6‑149 (MQ=255) taaaaaaaaaGCCCGGTTCCAAGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTACCCTGTCAGCAGTCAGTGGCCGGTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCTTGGCAGtgtatg > 3:176457/5‑149 (MQ=38) taaaaaaaaaGCCCGGTTCCAAGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCTTGTCAGtgtgtg > 3:61887/5‑149 (MQ=255) tCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCTTGTCAGtgtgtg > 4:185619/1‑149 (MQ=255) gAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCTTGTCAGTGTGTGGAt > 6:50585/1‑149 (MQ=255) aaGCCCGGTTCCAAGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCTTGTCAGTGTGTGGATTTGtt > 3:279077/1‑149 (MQ=255) gCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCTTGTCAGTGTGTGGATTTGTTTc < 8:174372/149‑1 (MQ=255) gCCCGGTTCCAAGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCTTGTCAGTGTGTGGATTTGTTTc < 8:61354/149‑1 (MQ=255) gTTCCAAGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCgggg > 7:193518/1‑113 (MQ=255) gTTCCAAGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCgggg < 8:193518/113‑1 (MQ=255) gTTCCAAGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCggg < 4:199986/112‑1 (MQ=255) gTTCCAAGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCggg > 3:199986/1‑112 (MQ=255) ttGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCTTGTCAGTGTGTGGATTTGTTTCTTACCATGAc > 6:125012/1‑149 (MQ=255) ttGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGAAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCTTGTCAGTGTGTGGATTTGTTTCTTACCATGAc > 8:130630/1‑149 (MQ=255) | CGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCG‑TAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGC‑TTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCTTGTCAGTGTGTGGATTTGTTTCTTACCATGAC > NC_002947/1503928‑1504215 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |