Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,521,248 C→A 9.1% Q169K (CAG→AAG)  murD → UDP‑N‑acetylmuramoylalanine‑‑D‑glutamate ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,521,2480CA 9.1% 133.1 / 4.4 44Q169K (CAG→AAG) murDUDP‑N‑acetylmuramoylalanine‑‑D‑glutamate ligase
Reads supporting (aligned to +/- strand):  ref base C (23/17);  new base A (2/2);  total (25/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.60e-01

GCCAAGAGCACCGTCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGCCAGGTGGTGGTCAACC  >  NC_002947/1521104‑1521395
                                                                                                                                                |                                                                                                                                                   
gCCAAGAGCACCGTCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTtccagcttccagct                                                                                                                                                 <  3:332363/149‑1 (MQ=255)
     gagCACCGTCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTGGAAGCTGGaaa                                                                                                                                            <  1:47382/149‑1 (MQ=255)
        cACCGTCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCa                                                                                                                                         <  5:324455/149‑1 (MQ=255)
          ccGTCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCTGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACt                                                                                                                                       <  6:345517/149‑1 (MQ=255)
          ccGTCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGATGaaacatata                                                                                                                                       >  7:223569/1‑143 (MQ=255)
                              gAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTGGAAGCTGGaaa                                                                                                                                            >  8:254028/1‑124 (MQ=255)
                              gAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTGGAAGCTGGaaa                                                                                                                                            <  7:254028/124‑1 (MQ=255)
                                     ccgccAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGAcc                                                                                                            >  4:57882/1‑149 (MQ=255)
                                         cAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACt                                                                                                                                       <  8:148832/118‑1 (MQ=255)
                                         cAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACt                                                                                                                                       >  7:148832/1‑118 (MQ=255)
                                                ggCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTg                                                                                                                                                >  4:157146/1‑102 (MQ=255)
                                                ggCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTg                                                                                                                                                <  3:157146/102‑1 (MQ=255)
                                                     aCGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGc                                                                                             >  6:64121/1‑148 (MQ=255)
                                                       gcgTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGaa                                                                                          <  5:288732/149‑1 (MQ=255)
                                                            gCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACca                                                                                     <  3:57882/149‑1 (MQ=255)
                                                              cGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACcaca                                                                                   >  8:255048/1‑149 (MQ=255)
                                                                      gTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGAcc                                                                                                                                   >  5:51923/1‑93 (MQ=255)
                                                                      gTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGAcc                                                                                                                                   <  6:51923/93‑1 (MQ=255)
                                                                          ccTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGAc                                                                                                                                    <  3:332164/88‑1 (MQ=255)
                                                                          ccTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGAc                                                                                                                                    >  4:332164/1‑88 (MQ=255)
                                                                           cTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATg                                                                                 <  1:163541/138‑1 (MQ=255)
                                                                           cTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAg                                                                      <  5:64121/149‑1 (MQ=255)
                                                                           cTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCCTTCAGAAAATAAACCAAGACCACATGGatcaacg                                                                         >  2:163541/1‑140 (MQ=255)
                                                                             cGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGa                                                                                           <  3:216347/126‑1 (MQ=255)
                                                                             cGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGa                                                                                           >  4:216347/1‑126 (MQ=255)
                                                                              ggTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGc                                                                     <  7:255048/147‑1 (MQ=255)
                                                                                   cccGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTc                                                              <  6:17230/149‑1 (MQ=255)
                                                                                   cccGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTc                                                              >  5:17230/1‑149 (MQ=255)
                                                                                         aCTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACca                                                                                     >  5:44140/1‑120 (MQ=255)
                                                                                         aCTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACca                                                                                     <  6:44140/120‑1 (MQ=255)
                                                                                          cTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGTCCTa                                                       >  5:68269/1‑149 (MQ=255)
                                                                                                            gACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCg                                     >  5:169909/1‑149 (MQ=255)
                                                                                                               aTCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGAt                                  >  1:162979/1‑149 (MQ=255)
                                                                                                                  gAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGAttt                                >  8:281243/1‑148 (MQ=255)
                                                                                                                     cTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCcg                            <  6:169909/149‑1 (MQ=255)
                                                                                                                           gTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGc                      >  2:169806/1‑149 (MQ=255)
                                                                                                                              cTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCg                   >  7:188925/1‑149 (MQ=255)
                                                                                                                               tGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTcc                             <  4:240565/138‑1 (MQ=255)
                                                                                                                               tGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTcc                             >  3:240565/1‑138 (MQ=255)
                                                                                                                               tGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGc                  >  6:336371/1‑149 (MQ=255)
                                                                                                                                ggAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGc                                                          >  2:173260/1‑108 (MQ=255)
                                                                                                                                ggAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGc                                                          <  1:173260/108‑1 (MQ=255)
                                                                                                                                 gAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGc                  >  1:140466/1‑147 (MQ=255)
                                                                                                                                     tttCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGCCAggtg            >  5:101502/1‑149 (MQ=255)
                                                                                                                                     tttCCAGCTGGAAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGCCAggtg            >  4:72794/1‑149 (MQ=255)
                                                                                                                                      ttccagcttccagcTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTTCGGCCTACCACCTGGCCAAacac                                       <  2:254762/121‑1 (MQ=255)
                                                                                                                                      ttccagcttccagcTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTTCGGCCTACCACCTGGCCAAacac                                       >  1:254762/1‑121 (MQ=255)
                                                                                                                                       tccagcttccagctGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATc                                                                                                <  6:230410/63‑1 (MQ=255)
                                                                                                                                       tccagcttccagctGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATc                                                                                                >  5:230410/1‑63 (MQ=255)
                                                                                                                                         cagcttccagctGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGCCAggtggtg         <  2:140466/148‑1 (MQ=255)
                                                                                                                                           gcttccagctGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGCCAGGTGGTGGTc      <  7:281243/149‑1 (MQ=255)
                                                                                                                                              tccagctGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGCCAGGTGGTGGTCAAc   >  4:85226/1‑149 (MQ=255)
                                                                                                                                                cagctGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACcaca                                                                                   <  2:140229/67‑1 (MQ=255)
                                                                                                                                                cagctGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACcaca                                                                                   >  1:140229/1‑67 (MQ=255)
                                                                                                                                                cagctGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGCCAGGTGGTGGTCAAcc  <  1:169806/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
GCCAAGAGCACCGTCACGACGCTGGTCGGTGAAATGGCCGCCAAGGCCGGCAAACGCGTGGCCGTGGGCGGTAACCTCGGTACCCCGGCACTCGACCTGCTGGCCGATGACATCGAGCTGTACGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACTGACCAGCTCAACGCCGAAGTGGCGACCGTTCTGAACATCAGCGAAGACCACATGGACCGCTATAGCGGCCTTCCGGCCTACCACCTGGCCAAACACCGGATTTTCCGCGGTGCCCGCCAGGTGGTGGTCAACC  >  NC_002947/1521104‑1521395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑