| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,542,895 | G→A | 6.4% | T225T (ACG→ACA) | PP_1353 → | membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,542,895 | 0 | G | A | 6.4% | 182.6 / 2.7 | 62 | T225T (ACG→ACA) | PP_1353 | membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (31/27); new base A (2/2); total (33/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
ACCGCCAGCCGACGCGCAAGGTGGTGTTCGATGTGGGCGTTGACTACGAGGCCGACCTGCAGAAGGCCCGCAATGTGTTGCTGGAACTGGCAAAGGACCCGCGTGTTCTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACATTCCGTTTCCGCAGCGGGTTATTC > NC_002947/1542753‑1543040 | agcGCCAGCCGTCGCGCAAGGTGGTGTTCGATGTGGGCGTTGACTACGAGGCCGACGTGCAGAAGGCCCGCAATGTGTTGCTGGAACTGGCAAAGGACCCGCGTTTTCTGCAAGACCCGTCACCGCAGGCGGTCGTGTCGACGCTGGGc < 3:251219/147‑1 (MQ=255)acCGCCAGCCGACGCGCAAGGTGGTGTTCGATGTGGGCGTTGACTACGAGGCCGACCTGCAGAAGGCCCGCAATGTGTTGCTGGAACTGGCAAAGGACCCGCGTGTTCTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTgg > 1:275552/1‑147 (MQ=255)acCGCCAGCCGACGCGCAAGGTGGTGTTCGATGTGGGCGTTGACTACGAGGCCGACCTGCAGAAGGCCCGCAATGTGTTGCTGGAACTGGCAAAGGACCCGCGTGTTCTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTgg < 2:275552/147‑1 (MQ=255) gtTCGATGTGGGCGTTGACTACGAGGCCGACCTGCAGAAGGCCCGCAATGTGTTGCTGGAACTGGCAAAGGACCCGCGTGTTCTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTgc > 4:319041/1‑149 (MQ=255) aTGTGGGCGTTGACTACGAGGCCGACCTGCAGAAGGCCCGCAATGTGTTGCTGGAACTGGCAAAGGACCCGCGTGTTCTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTtg > 5:25808/1‑149 (MQ=255) gCGTTGACTACGAGGCCGACCTGCAGAAGGCCCGCAATGTGTTGCTGGAACTGGCAAAGGACCCGCGTGTTCTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGAcc < 8:196380/149‑1 (MQ=255) ttGACTACGAGGCCGACCTGCAGAAGGCCCGCAATGTGTTGCTGGAACTGGCAAAGGACCCGCGTGTTCTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAg < 3:319041/149‑1 (MQ=255) cTACGAGGCCGACCTGCAGAAGGCCCGCAATGTGTTGCTGGAACTGGCAAAGGACCCGCGTGTTCTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCt > 7:311267/1‑149 (MQ=255) gAGGCCGACCTGCAGAAGGCCCGCAATGTGTTGCTGGAACTGGCAAAGGACCCGCGTGTTCTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGa > 2:67049/1‑149 (MQ=255) ccGACCTGCAGAAGGCCCGCAATGTGTTGCTGGAACTGGCAAAGGACCCGCGTGTTCTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTAc < 1:67049/149‑1 (MQ=255) gACCTGCAGAAGGCCCGCAATGTGTTGCTGGAACTGGCAAAGGACCCGCGTGTTCTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTtgtg < 4:264961/128‑1 (MQ=255) gACCTGCAGAAGGCCCGCAATGTGTTGCTGGAACTGGCAAAGGACCCGCGTGTTCTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTtgtg > 3:264961/1‑128 (MQ=255) cAGAAGGCCCGCAATGTGTTGCTGGAACTGGCAAAGGACCCGCGTGTTCTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGAc < 8:311267/147‑1 (MQ=255) aGAAGGCCCGCAATGTGTTGCTGGAACTGGCAAAGGACCCGCGTGTTCTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGACGt > 1:64917/1‑148 (MQ=255) cccGCAATGTGTTGCTGGAACTGGCAAAGGACCCGCGTGTTCTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGACGTGATGtt > 6:361158/1‑148 (MQ=255) ccGCAATGTGTTGCTGGAACTGGCAAAGGACCCGCGTGTTCTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGACGTGATGTTCa > 5:27023/1‑149 (MQ=255) tGGAACTGGCAAAGGACCCGCGTGTTCTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGc < 8:211300/63‑1 (MQ=255) gCAAAGGACCCGCGTGTTCTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGa < 2:327478/107‑1 (MQ=255) gCAAAGGACCCGCGTGTTCTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGa > 4:282318/1‑149 (MQ=255) gCAAAGGACCCGCGTGTTCTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGa > 4:208806/1‑149 (MQ=255) aaGGACCCGCGTGTTCTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCg < 2:248759/149‑1 (MQ=255) cccGCGTGTTCTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTg > 3:128130/1‑148 (MQ=255) ccGCGTGTTCTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTtgtg < 8:37405/83‑1 (MQ=255) ccGCGTGTTCTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTtgtg > 7:37405/1‑83 (MQ=255) ccGCGTGTTCTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACt < 3:94669/103‑1 (MQ=255) ccGCGTGTTCTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACt > 4:94669/1‑103 (MQ=255) ccGCGTGTTCTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGACGTg < 1:65981/111‑1 (MQ=255) ccGCGTGTTCTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGACGTg > 2:65981/1‑111 (MQ=255) ccGCGTGTTCTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGACGTGATGTTCATGCTCAACGa < 8:217793/128‑1 (MQ=255) ccGCGTGTTCTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGACGTGATGTTCATGCTCAACGa > 7:217793/1‑128 (MQ=255) ccGCGTGTTCTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTg > 8:171512/1‑147 (MQ=255) ccGCGTGTTCTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTg > 4:314873/1‑147 (MQ=255) ccGCGTGTTCTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGaa < 3:314873/149‑1 (MQ=255) ccGCGTGTTCTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGaa < 6:27023/149‑1 (MQ=255) cgcgTGTTCTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAg < 4:128130/149‑1 (MQ=255) gcgTGTTCTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAgg > 5:181135/1‑149 (MQ=255) ttCTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCg > 4:254364/1‑147 (MQ=255) ttCTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGaa < 2:64917/149‑1 (MQ=255) cTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAg > 6:72400/1‑141 (MQ=255) cTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAg < 5:72400/141‑1 (MQ=255) cTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAAg < 7:171512/148‑1 (MQ=255) cAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGACGTGAt < 2:267090/101‑1 (MQ=255) cAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGCACGTGATGTTCATGCTCAACGACTATGCGCGTCACCGGTTGAAGGCCGAAGGGAt > 2:327995/1‑149 (MQ=255) cAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACAGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGACGTGAt > 1:267090/1‑101 (MQ=255) cAAGACCCGGCACCGCAGGCGGTCGTGTCGACACTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGAt > 8:297879/1‑149 (MQ=255) cAAGACCCGGCACCGCAGGCGGTCGTGTCGACACTGGGCGACAGCTCGATCAACGTATCGCTGCGCTTGTGGACCTAGACCTCCGATTACTGGGACGTGATGTTCATGCTCAACGAGTTTTCTCGTGACCGGTTGATGGCCGAAGGGa > 8:293193/1‑148 (MQ=255) gACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCg > 3:336163/1‑148 (MQ=255) cccGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACa > 7:257852/1‑149 (MQ=255) ccGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAg > 3:156608/1‑132 (MQ=255) ccGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAg < 4:156608/132‑1 (MQ=255) cGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCCTGTGGACCAAGACCTCCGATTACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACATTCCGttt < 5:361158/149‑1 (MQ=255) cGCAGGCGGTCGTGTCGACACTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACATTCCGttt < 7:297879/149‑1 (MQ=255) cGCAGGCGGTCGTGTCGACACTGGGCGACAGCTCGATCACCGTATCGATTCGCTTGTGGACCAAGACCTCCGATTACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACATTCCGttt < 7:293193/149‑1 (MQ=255) gCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGACGTGATGTTCATGCTCAACGAGTATg < 1:12722/107‑1 (MQ=255) gCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGACGTGATGTTCATGCTCAACGAGTATg > 2:12722/1‑107 (MQ=255) gCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACATTCCGTTTc < 3:208806/149‑1 (MQ=255) gCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGACGTGATGTTCATGCTCAACGAGt < 4:190472/100‑1 (MQ=255) gCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGACGTGATGTTCATGCTCAACGAGt > 3:190472/1‑100 (MQ=255) tCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACATTCCGTTTCCGCAGCgg > 6:136414/1‑149 (MQ=255) gtCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAACGGATCGACATTCCGTTTCCCCAGCGGGTTa > 7:257168/1‑149 (MQ=255) tCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACATTCCGTTTCCGCAGCGGGTTAt > 6:95106/1‑149 (MQ=255) gACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACATTCCGTTTCCGCAGCGGGTTATTc < 8:257168/149‑1 (MQ=255) | ACCGCCAGCCGACGCGCAAGGTGGTGTTCGATGTGGGCGTTGACTACGAGGCCGACCTGCAGAAGGCCCGCAATGTGTTGCTGGAACTGGCAAAGGACCCGCGTGTTCTGCAAGACCCGGCACCGCAGGCGGTCGTGTCGACGCTGGGCGACAGCTCGATCACCGTATCGCTGCGCTTGTGGACCAAGACCTCCGATTACTGGGACGTGATGTTCATGCTCAACGAGTATGCGCGTGACCGGTTGAAGGCCGAAGGGATCGACATTCCGTTTCCGCAGCGGGTTATTC > NC_002947/1542753‑1543040 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |