| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,562,170 | C→G | 15.4% | intergenic (‑80/‑89) | pctA ← / → PP_1372 | methyl‑accepting chemotaxis protein PctA/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,562,170 | 0 | C | G | 15.4% | 56.3 / 5.1 | 26 | intergenic (‑80/‑89) | pctA/PP_1372 | methyl‑accepting chemotaxis protein PctA/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (10/11); new base G (2/2); total (12/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.84e-01 | |||||||||||
GAGTATCACGATCAGTGATGCCGCCAAAAGAATCTTGTGGCTGAAACGAAGGCTTTTGTTCATGGCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGTCCCTTTTC‑GGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCAGAGGATTAAAGAGATGTCGGAATTTTCGACCATCGTTGTAGGCGCGGGCCCGGATGGCCAGCCAGTCGGGC > NC_002947/1562028‑1562316 | gAGTATCACGATCAGTGATGCCGCCAAAAGAATCTTGTGGCTGAAACGAAGGCTTTTGTTCATGGCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGTCCCtttt > 2:245670/1‑149 (MQ=255) ccgatctaTCAGTGATGCCGCCAAAAGAATCTTGTGGCTGAAACGAAGGCTTTTGTTCATGGCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGTCCCTTTTC‑gg < 7:162901/142‑1 (MQ=255) tCACGATCAGTGATGCCGCCAAAAGAATCTTGTGGCTGAAACGAAGGCTTTTGTTCATGGCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGGGACTTTTC‑ggga < 5:105950/149‑3 (MQ=255) tCAGTGATGCCGCCAAAAGAATCTTGTGGCTGAAACGAAGGCTTTTGTTCATGGCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGTCCCTTTTC‑gg > 8:162901/1‑141 (MQ=255) aaaaGAATCTTGTGGCTGAAACGAAGGCTTTTGTTCATGGCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGTCCCTTTTC‑GGCTGTTCCTCTCTATTAAGGc < 4:300019/147‑1 (MQ=255) aaaaGAATCTTGTGGCTGAAACGAAGGCTTTTGTTCATGGCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGTCCCTTTTC‑GGCTGTTCCTCTCTATTAAGGc > 3:300019/1‑147 (MQ=255) aaaGAATCTTGTGGCTGAAACGAAGGCTTTTGTTCATGGCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGTCCCTTTTC‑GGCTGTTCctctc > 1:400/1‑137 (MQ=255) aaaGAATCTTGTGGCTGAAACGAAGGCTTTTGTTCATGGCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGTCCCTTTTC‑GGCTGTTCctctc < 2:400/137‑1 (MQ=255) aaGAATCTTGTGGCTGAAACGAAGGCTTTTGTTCATGGCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGTCCCTTTTC‑GGCTGTTCCTCTCTATTAAGGCGAAt < 8:64265/149‑1 (MQ=255) gAATCTTGTGGCTGAAACGAAGGCTTTTGTTCATGGCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGTCCCTTTTC‑GGCTGTTCCTCTCTATTAAGGCGAATaa < 1:245670/149‑1 (MQ=255) cTGAAACGAAGGCTTTTGTTCATGGCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGTCCCTTTTC‑GGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAgg > 6:200060/1‑148 (MQ=255) tGAAACGAAGGCTTTTGTTCATGGCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGGGACTTTTC‑gg > 6:63316/1‑111 (MQ=255) ttGTTCATGGCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGTCCCTTTTC‑GGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTaa < 8:273917/148‑1 (MQ=255) ttCATCGCGTACTCGCTTCCGCTAAGCGTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGTCCCTTTTC‑GGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCt < 6:82019/148‑1 (MQ=255) aTCGGTTCCGCTATTGTTTTATATGAGGCTTTCGCACGAGTTGAGCCGTAGGTTGTGCAACATCCAGAAAATTACCTTTTC‑GGCTGTTCCTATCTATTAAGGCTAATAACACCCACAGGTATCGGCCTAATTAATCTAAAGCTTGAga < 4:97002/148‑2 (MQ=255) cGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGTCCCTTTTC‑GGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAg < 1:162146/110‑1 (MQ=255) cGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGTCCCTTTTC‑GGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAg > 2:162146/1‑110 (MQ=255) cggCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGTCCCTTTTC‑GGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAg < 4:278313/115‑1 (MQ=255) aGGTTGTGCAACATCCCGAAAAGTCCCTTTTC‑GGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCAGAGGATTAAAGAGATGTCGGAATTTTCGACCATCGTTGTAGGCGCAGGc > 1:280648/1‑149 (MQ=255) aaCATCCCGAAAAGTCCCTTTTC‑GGCTGTTCCTCACTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCAGAGGATTAAAGAGATGTCGGAATTTTCGACCATCGTTGTAGGAGCGGGCCCGGaagtc > 2:234344/1‑145 (MQ=255) aTCCCGAAAAGTCCCTTTTC‑GGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCAGAGGATTAAAGAGATGTCGGAATTTTCGACCATCGTTGTAGGCGCGGGCCCGGATGgccag > 7:304950/1‑149 (MQ=255) tCCCGAAAAGTCCCTTTTC‑GGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCAGAGGATTAAAGAGATGTCGGAATTTTCGACCATCGTTGTAGGCGCGGGCCCGGATGgccagc < 8:304950/149‑1 (MQ=255) gccGAAAAGGGACTTTTCGGGATGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCAGAGGATTAAAGAGATGTCGGAATTTTCGACCATCATTGTAGGCGCGGGCCCGGAt < 1:342577/141‑1 (MQ=255) gccGAAAAGGGACTTTTCGGGATGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCAGAGGATTAAAGAGATGTCGGAATTTTCGACCATCATTGTAGGCGCGGGCCCGGAt > 2:342577/2‑142 (MQ=255) gTCCCTTTTC‑GGCTGTTCCTCTCTATTAAGGCTAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCAGAGGATTAATGAGATGTCGGAATTTTCGACCATCGTTGTAGGCGCGGGCCCGGATGGCCAGCCAGTCGGgc < 1:234344/149‑1 (MQ=255) tCCCTTTTC‑GGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCAGAGGATTAAAGAGATGTCGGAATTTTCGACCATCGTTGTAGGCGCGGGCCCGGATGGCCAGCCAGTCGGgc > 7:316188/1‑148 (MQ=255) | GAGTATCACGATCAGTGATGCCGCCAAAAGAATCTTGTGGCTGAAACGAAGGCTTTTGTTCATGGCGTAATCGGTTCCGCTAAGGTTTTATATCAGGCGTTCGCACGAGTGGAGCCGTAGGTTGTGCAACATCCCGAAAAGTCCCTTTTC‑GGCTGTTCCTCTCTATTAAGGCGAATAACACCCACAGGTATCGGCCGAATTAAGCTAAAGCTTGAGCAGAGGATTAAAGAGATGTCGGAATTTTCGACCATCGTTGTAGGCGCGGGCCCGGATGGCCAGCCAGTCGGGC > NC_002947/1562028‑1562316 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |