Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,564,931	A→T	7.9%	L133Q (CTG→CAG)	pitB ←	phosphate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,564,931	0	A	T	7.9%	182.3 / 4.3	63	L133Q (CTG→CAG)	pitB	phosphate transporter
Reads supporting (aligned to +/- strand):  ref base A (30/28);  new base T (2/3);  total (32/31)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

GCGCCACCATTTCAGGCCGATCAGCACCAGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATGGCCAGGCCGTGGCCGGTGTTCACATTGATCAG  >  NC_002947/1564786‑1565076                                                                                                                                                  |                                                                                                                                                  gcgcCACCATTTCAGGCCGATCAGCACCAGGGCAGCAACGGCGAAGCCGTCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTggccaggc                                                                                                                                                <  5:165516/149‑1 (MQ=255)     caccaTTTCAGGCCGATCAGCACCAGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCaa                                                                                                                                             >  1:294202/1‑148 (MQ=255)         aTTTCAGGCCGATCAGCACCAGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTggccagg                                                                                                                                                 <  8:176139/140‑1 (MQ=255)         aTTTCAGGCCGATCAGCACCAGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTggccagg                                                                                                                                                 >  7:176139/1‑140 (MQ=255)              aGGCCGATCAGCACCAGGGCAGCAACGGCTAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGAt                                                                                                                                   <  5:332132/149‑1 (MQ=255)               ggCCGATCAGCACCAGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATg                                                                                                                                  <  1:322192/149‑1 (MQ=255)                gCCGATCAGCACCAGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATgg                                                                                                                                 <  6:48225/149‑1 (MQ=255)                    aTCAGCACCAGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAAc                                                                                                                              >  6:158345/1‑148 (MQ=255)                    aTCAGCACCAGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAAc                                                                                                                              <  5:158345/148‑1 (MQ=255)                        gCACCAGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAac                                                                                                                                            >  6:106210/1‑130 (MQ=255)                        gCACCAGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAac                                                                                                                                            <  5:106210/130‑1 (MQ=255)                         cACCAGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGAt                                                                                                                          >  6:25191/1‑147 (MQ=255)                         cACCAGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGAt                                                                                                                          <  5:25191/147‑1 (MQ=255)                          aCCAGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAg                                                                                                                       >  6:22044/1‑149 (MQ=255)                          aCCAGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAg                                                                                                                       >  6:16881/1‑149 (MQ=255)                           ccAGGGCAGCAACGGCGAAGCCGTCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGc                                                                                                                      <  5:20293/149‑1 (MQ=255)                            cAGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGaagaatcgg                                                                                                                        >  5:342255/1‑139 (MQ=255)                            cAGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCg                                                                                                                     >  6:221140/1‑149 (MQ=255)                             aGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCgt                                                                                                                    >  5:40473/1‑149 (MQ=255)                             aGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCgt                                                                                                                    <  5:22044/149‑1 (MQ=255)                             aGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCgt                                                                                                                    <  5:16881/149‑1 (MQ=255)                                    caACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGaa                                                                                                             <  5:221140/149‑1 (MQ=255)                                             aGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGaa                                                                                                             <  5:122845/140‑1 (MQ=255)                                             aGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGaa                                                                                                             >  6:122845/1‑140 (MQ=255)                                                      ttGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGcc                                                                                            >  4:118200/1‑148 (MQ=255)                                                         gCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCaaa                                                                                         <  3:118200/148‑1 (MQ=255)                                                         gCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAg                                                                                        >  7:83149/1‑149 (MQ=255)                                                           gagaCCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTa                                                                                      >  3:258513/1‑149 (MQ=255)                                                                caccaGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAgg                                                                                 >  6:130717/1‑149 (MQ=255)                                                                   caGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGc                                                                              >  1:79514/1‑149 (MQ=255)                                                                     gCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGcc                                                                             >  3:131840/1‑148 (MQ=255)                                                                       gACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAg                                                                          <  3:60005/149‑1 (MQ=255)                                                                       gACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGACCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAg                                                                                                       >  2:45984/1‑120 (MQ=255)                                                                       gACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGACCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAg                                                                                                       <  1:45984/120‑1 (MQ=255)                                                                        aCATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAgg                                                                         <  2:79514/149‑1 (MQ=255)                                                                               gATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCaa                                                                                          <  6:212351/125‑1 (MQ=255)                                                                               gATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCaa                                                                                          >  5:212351/1‑125 (MQ=255)                                                                                  atcgatcgCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCa                                                                                   >  4:193183/1‑129 (MQ=255)                                                                                  atcgatcgCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCa                                                                                   <  3:193183/129‑1 (MQ=255)                                                                                     gatcgCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCCATCAGCGAGTGCGAACTGCAGGCGGGCATGCCa                                                                                           >  7:298677/1‑118 (MQ=255)                                                                                     gatcgCGTTCTGCGAGTTGACGCCGTCGGCGAGCGGAATGTCGTTGATCAGGGCGTTGGCGAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCa                                                                                           <  8:298677/118‑1 (MQ=255)                                                                                           tttCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGcag                                                      >  3:88294/1‑149 (MQ=255)                                                                                           tttCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGcag                                                      >  7:226311/1‑149 (MQ=255)                                                                                                    gTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGt                                                                        >  2:39076/1‑122 (MQ=255)                                                                                                       gACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACgag                                           >  6:339082/1‑148 (MQ=255)                                                                                                          gCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGagaag                                        >  6:125412/1‑148 (MQ=255)                                                                                                          gCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGagaag                                        <  5:125412/148‑1 (MQ=255)                                                                                                             gTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGcagc                                                     <  3:188190/132‑1 (MQ=255)                                                                                                             gTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGcagc                                                     >  4:188190/1‑132 (MQ=255)                                                                                                                gCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATgg                                 <  4:258513/149‑1 (MQ=255)                                                                                                                gCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATg                                  <  4:131840/148‑1 (MQ=255)                                                                                                                 cccAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATGGc                                <  5:339082/149‑1 (MQ=255)                                                                                                                   cAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCa                                                                    >  3:73390/1‑111 (MQ=255)                                                                                                                   cAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCa                                                                    <  4:73390/111‑1 (MQ=255)                                                                                                                    cttccgaTGTCGTTGATCAGGGCGTGGGCCTGGCCAAGACCGAGTATGGAACCGATCAGCGTGTGCGAACTGGAGGGGTTGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCTACGAGAAGACCATGGCCAg                             <  7:154822/143‑1 (MQ=255)                                                                                                                         aaTGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCTGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATGGCCAGGCCGt                        <  6:40473/149‑1 (MQ=255)                                                                                                                               gTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCATCCAGCAACGAGAACACCATGGCCat                             >  8:154822/1‑137 (MQ=255)                                                                                                                                      tcggtgTTGGCCTGGCCAACGCCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATGGCCAgg                            >  4:346505/6‑132 (MQ=255)                                                                                                                                      tcggtgTTGGCCTGGCCAACGCCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATGGCCAgg                            <  3:346505/127‑1 (MQ=255)                                                                                                                                      aGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATGGCCACGGCCTGGccatg                 >  6:253883/1‑140 (MQ=255)                                                                                                                                       gggCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATg                                                             >  5:7746/1‑98 (MQ=255)                                                                                                                                       gggCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATg                                                             <  6:7746/98‑1 (MQ=255)                                                                                                                                        ggtgTTGGCCTGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATGGCCAGGCCGTGGCCGGTGTTCACAt         >  7:302952/4‑149 (MQ=255)                                                                                                                                        ggtgTTGGCCTGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATGGCCAGGCCGTGGCCGGTGTTCACAt         <  8:302952/146‑1 (MQ=255)                                                                                                                                         gCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATGGCCAGGCCGTGGCCGGTGTTCACAtt        <  2:294202/149‑1 (MQ=255)                                                                                                                                         gCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATGGCCAGGCCGTGGCCGGTGTTCACAtt        <  4:88294/149‑1 (MQ=255)                                                                                                                                               gccaggccaACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGAt                                                              <  4:339229/89‑1 (MQ=255)                                                                                                                                               gccaggccaACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGAt                                                              >  3:339229/1‑89 (MQ=255)                                                                                                                                                 caggccaACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATGGCCAGGCCGTGGCCGGTGTTCACATTGATcag  >  5:116799/1‑147 (MQ=255)                                                                                                                                                  |                                                                                                                                                  GCGCCACCATTTCAGGCCGATCAGCACCAGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATGGCCAGGCCGTGGCCGGTGTTCACATTGATCAG  >  NC_002947/1564786‑1565076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑