Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,578,350 A→G 8.1% I1016T (ATC→ACC)  ttgB ← efflux pump membrane protein TtgB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,578,3500AG 8.1% 131.5 / 3.1 49I1016T (ATC→ACC) ttgBefflux pump membrane protein TtgB
Reads supporting (aligned to +/- strand):  ref base A (26/19);  new base G (2/2);  total (28/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

AAGCGGTTACCGCCAGGGACAACAAAGACTTGGTCATTGCCCAGCCTCATAACGTGGATTTTCAGGGGTGACGTCTTTTTCCGGCTCTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGAACGCCAGCGAGGTCATGATGATCGGGCGCA  >  NC_002947/1578211‑1578491
                                                                                                                                           |                                                                                                                                             
aaGCGGTTACCGCCAGGGACAACAAAGACTTGGTCATTGCCCAGCCTCATAACGTGGATTTTCAGGGGTGACGTCTTTTTCCGGCTCTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCa                                                                                                                                         <  3:172127/146‑1 (MQ=255)
aaGCGGTTACCGCCAGGGACAACAAAGACTTGGTCATTGCCCAGCCTCATAACGTGGATTTTCAGGGGTGACGTCTTTTTCCGGCTCTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCa                                                                                                                                         >  4:172127/1‑146 (MQ=255)
             cAGGGACAACAAAGACTTGGTCATTGCCCAGCCTCATAACGTGGATTTTCAGGGGTGACGTCTTTTTCCGGCTTTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGATGATAGCCAGCACGGTCGCACTGAt                                                                                                                         >  6:132389/1‑149 (MQ=255)
                       aaaGACTTGGTCATTGCCCAGCCTCATAACGTGGATTTTCAGGGGTGACGTCTTTTTCCGGCTCTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATgccgcc                                                                                                                >  3:345951/1‑148 (MQ=255)
                        aaGACTTGGTCATTGCCCAGCCTCATAACGTGGATTTTCAGGGGTGACGTCTTTTTCCGGCTTTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGATGATAGCCAGCACGGTCGCACTGATCATGCCGCCGa                                                                                                              <  5:132389/149‑1 (MQ=255)
                             ttGGTCATTGCCCAGCCTCATAACGTGGATTTTCAGGGGTGACGTCTTTTTCCGGCTCTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACg                                                                                                         >  8:139461/1‑149 (MQ=255)
                               ggTCATTGCCCAGCCTCATAACGTGGATTTTCAGGGGTGACGTCTTTTTCCGGCTCTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGc                                                                                                        <  7:139461/148‑1 (MQ=255)
                                                     gTGGATTTTCAGGGGTGACGTCTTTTTCCGGCTCTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTgctggctg                                                                                 >  3:198411/1‑149 (MQ=255)
                                                     gTGGATTTTCAGGGGTGACGTCTTTTTCCGGCTCTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTgctggctg                                                                                 >  2:60521/1‑149 (MQ=255)
                                                      tGGATTTTCAGGGGTGACGTCTTTTTCCGGCTCTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACACAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTgc                                                                                >  3:149896/1‑149 (MQ=255)
                                                        gATTTTCAGGGGTGACGTCTTTTTCCGGCTCTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTgccg                                                                              <  1:60521/149‑1 (MQ=255)
                                                                    tGACGTCTTTTTCCGGCTCTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGCCGTGCTGGCTGCCGgcgccggcgccg                                                                  >  3:11515/1‑149 (MQ=255)
                                                                        gTCTTTTTCCGGCTCTTTGCTGCCGAACAGCGACGACACTGTGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGa                                                                                               <  6:364148/116‑1 (MQ=255)
                                                                           tttttCCGGCTCTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGa                                                           >  6:307341/1‑149 (MQ=255)
                                                                           tttttCCGGCTCTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGACGCCGATCACGCCAGTAACGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCg                                                            >  1:141309/1‑148 (MQ=255)
                                                                             tttCCGGCGATTTGCTGCCGAACAGCGACTACACTGCGACGAAGAACAGTGGTACCCTGAAGATAGACAGCACGGTCGCACTGATCATGCCGCCGATCACGCCTGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCg                                                            <  2:141309/146‑1 (MQ=255)
                                                                                ccGGCTCTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATg                                                         >  1:102668/1‑146 (MQ=255)
                                                                                ccGGCTCTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATg                                                         <  2:102668/146‑1 (MQ=255)
                                                                                  ggCTCTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCaa                                                    >  6:148720/1‑149 (MQ=255)
                                                                                  ggCTCTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCaa                                                    >  6:28956/1‑149 (MQ=255)
                                                                                         tgctgcCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGt                                                <  4:198411/146‑1 (MQ=255)
                                                                                          gctgcCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCa                                            >  4:269751/1‑149 (MQ=255)
                                                                                             gcCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTgg                                                              >  7:164078/1‑128 (MQ=255)
                                                                                             gcCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTgg                                                              <  8:164078/128‑1 (MQ=255)
                                                                                              ccGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGcc                                        >  4:262015/1‑149 (MQ=255)
                                                                                                  aCAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCa                                      <  4:11515/147‑1 (MQ=255)
                                                                                                       gacgacACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGaa                               >  1:253571/1‑149 (MQ=255)
                                                                                                        acgacACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAAc                                                   >  2:51228/1‑128 (MQ=255)
                                                                                                          gacACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGAACGc                            <  2:253571/149‑1 (MQ=255)
                                                                                                             acTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGAACGCCAg                         >  3:91509/1‑149 (MQ=255)
                                                                                                               tGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAgg                                    <  4:232902/136‑1 (MQ=255)
                                                                                                               tGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAgg                                    >  3:232902/1‑136 (MQ=255)
                                                                                                                gCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGAACGCCAGc                        >  4:133182/1‑147 (MQ=255)
                                                                                                                 cgacgaAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGAACGCCAGCGAg                     >  8:46569/1‑149 (MQ=255)
                                                                                                                  gacgaAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGAACGCCAGCGAgg                    >  3:58768/1‑149 (MQ=255)
                                                                                                                  gacgaAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGAACGCCAGCGAgg                    <  3:269751/149‑1 (MQ=255)
                                                                                                                    cgaAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGc                                         <  1:139209/126‑1 (MQ=255)
                                                                                                                        gaaCAGTGGTCCCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGAACGCCAGCGAGGTCatga              <  5:148720/149‑1 (MQ=255)
                                                                                                                        gaaCAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGAACGCCAGCGAGGTCatga              <  5:28956/149‑1 (MQ=255)
                                                                                                                        gaaCAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGAACGCCAGCGAGGTCatga              <  4:58768/149‑1 (MQ=255)
                                                                                                                        gaaCAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTCCCGGCGCAGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGCATGACAGTCAACGAGGTCatca              >  1:293395/1‑147 (MQ=255)
                                                                                                                            aGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGAACGCCAGCGAGGTCATgatgat          >  4:299987/1‑149 (MQ=255)
                                                                                                                                 tACCCAGAAGATAGCCCGCACGGTCGCACTGCTCATGCCGCCGATCACGCCAGTACCGATGGCGGGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGAACGCCAGCGAGGTCATGATGATCGGgc     <  2:108083/149‑1 (MQ=255)
                                                                                                                                 tACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGAACGCCAGCGAGGTCATGATGATCGGgc     <  3:133182/149‑1 (MQ=255)
                                                                                                                                   cccAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGAACGCCAGCGAGGTCATGATGATCGGgcg    >  5:155196/1‑148 (MQ=255)
                                                                                                                                     cAGAAGGTAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGAACGCCAGCGAGGTCATGATGATCGGgcg    >  8:334077/1‑146 (MQ=255)
                                                                                                                                     cAGAAGGTAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGAACGCCAGCGAGGTCATGATGATCGGGCGCa  <  7:278855/148‑1 (MQ=255)
                                                                                                                                     cAGAAGGTAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGAACGCCAGCGAGGTCATGATGATCGGGCGCa  <  7:334077/148‑1 (MQ=255)
                                                                                                                                     cAGAAGGTAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGAACGCCAGCGAGGTCATGATGATCGGGCGCa  >  8:278855/1‑148 (MQ=255)
                                                                                                                                           |                                                                                                                                             
AAGCGGTTACCGCCAGGGACAACAAAGACTTGGTCATTGCCCAGCCTCATAACGTGGATTTTCAGGGGTGACGTCTTTTTCCGGCTCTTTGCTGCCGAACAGCGACGACACTGCGACGAAGAACAGTGGTACCCAGAAGATAGCCAGCACGGTCGCACTGATCATGCCGCCGATCACGCCAGTACCGATGGCGTGCTGGCTGCCGGCGCCGGCGCCGCTGGCGATGGTCAACGGTACCACGCCCAGGATGAACGCCAGCGAGGTCATGATGATCGGGCGCA  >  NC_002947/1578211‑1578491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑