Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,595,911 G→A 6.9% A367V (GCG→GTG)  dctD‑III ← C4‑dicarboxylate transport transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,595,9110GA 6.9% 172.0 / 2.3 59A367V (GCG→GTG) dctD‑IIIC4‑dicarboxylate transport transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (25/29);  new base A (2/2);  total (28/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

ACCGACTTGATCTCGCCCCCATGCTGACGCAAGGCCGCACGCAGGCATTGCGCCTCGAATGCTTCCATCTGCTCGCCCAGCGACTGCCCGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTGCCAGGCCAGTCATGTGACAGCAGCTGCGCCAGCTGTGCACCCGATAGCGGCGGGGCCGCGCGACCAAGCTTTTCACCGGCAGCACGGGCAAAGTGTTCGAACAGCAGCGGAATGTCCTCCCGCC  >  NC_002947/1595767‑1596058
                                                                                                                                                |                                                                                                                                                   
aCCGACTTGATCTCGCCCCCATGCTGACGCAAGGCCGCACGCAGGCATTGCGCCTCGAATGCTTCCATCTGCTCGCCCAGCGACTGCCCGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCAtt                                                                                                                                                 >  8:206424/1‑149 (MQ=255)
 ccGACTTGATCTCGCCCCCATGCTGACGCAAGGCCGCACGCAGGCATTGCGCCTCGAATGCTTCCATCTGCTCGCCCAGCGACTGCCCGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTg                                                                                                                                                >  6:234798/1‑149 (MQ=255)
   gACTTGATCTCGCCCCCATGCTGACGCAAGGCCGCACGCAGGCATTGCGCCTCGAATGCTTCCATCTGCTCGCCCAGCGACTGCCCGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGc                                                                                                                                              <  6:151474/149‑1 (MQ=255)
        gATCTCGCCCCCATGCTGACGCAAGGCCGCACGCAGGCATTGCGCCTCGAATGCTTCCATCTGCTCGCCCAGCGACTGCCCGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGtt                                                                                                                                         >  4:298424/1‑149 (MQ=255)
        gATCTCGCCCCCATGCTGACGCAAGGCCGCACGCAGGCATTGCGCCTCGAATGCTTCCATCTGCTCGCCCAGCGACTGCCCGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGtt                                                                                                                                         >  7:35506/1‑149 (MQ=255)
         aTCTCGCCCCCATGCTGACGCAAGGCCGCACGCAGGCATTGCGCCTCGAATGCTTCCATCTGCTCGCCCAGCGACTGCCCGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGtt                                                                                                                                         <  7:206424/148‑1 (MQ=255)
            tcGCCCCCATGCTGACGCAAGGCCGCACGCAGGCATTGCGCCACGAATGCTTCCATCTGCTCGCCCAGCGTCTGCCCGGCACGTGCCCCCTCGATATTAGGCGAACACCCCCCCAACGCATGCCGCACCGCCCCAATGGCCAGTTcacg                                                                                                                                     >  5:168726/1‑149 (MQ=255)
                    aTGCTGACGCAAGGCCGCACGCAGGCATTGCGCCTCGAATGCTTCCATCTGCTCGCCCAGCGACTGCCCGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTgc                                                                                                                             <  1:307390/149‑1 (MQ=255)
                    aTGCTGACGCAAGGCCGCACGCAGGCATTGCGCCTCGAATGCTTCCATCTGCTCGCCCAGCGACTGCCCGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTgc                                                                                                                             >  7:24764/1‑149 (MQ=255)
                       cTGACGCAAGGCCGCACGCAGGCATTGCGCCTCGAATGCTTCCATCTGCTCGCCCAGCGACTGCCCGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTgccag                                                                                                                          <  8:177456/149‑1 (MQ=255)
                             cAAGGCCGCCCGCAGGCATTGCGCCTCGAATGCTTCCTTCTGCTCGCCCAGCGACTGCCCGGCTGGTGCCACCTCGATTTTTGGCGAACCCAGCCCCTACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTGCCTGGCGAGt                                                                                                                    <  6:168726/149‑1 (MQ=255)
                              aaGGCCGCACGCAGGCATTGCGCCTCGAATGCTTCCATCTGCTCGCCCAGCGACTGCCCGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTGCCAGGCCAGTc                                                                                                                   <  8:24764/149‑1 (MQ=255)
                                ggCCGCACGCAGGCATTGCGCCTCGAATGCTTCCATCTGCTCGCCCAGCGACTGCCCGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTGCCAGGCCAGTCAt                                                                                                                 <  3:298424/149‑1 (MQ=255)
                                        gcaGGCATTGCGCCTCGAATGCTTCCATCTGCTCGCCCAGCGACTGCCCGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTGCCAGGCCAGTCa                                                                                                                  >  3:273926/1‑140 (MQ=255)
                                        gcaGGCATTGCGCCTCGAATGCTTCCATCTGCTCGCCCAGCGACTGCCCGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTGCCAGGCCAGTCa                                                                                                                  <  4:273926/140‑1 (MQ=255)
                                             cATTGCGCCTCGAATGCTTCCATCTGCTCGCCCAGCGACTGCCCGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTGCCAGGCCAGTCATGTGACAGCAGCTg                                                                                                    >  8:10057/1‑149 (MQ=255)
                                             cATTGCGCCTCGAATGCTTCCATCTGCTCGCCCAGCGACTGCCCGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTGCCAGGCCAGTCATGTGACAGCAGCTg                                                                                                    >  8:229920/1‑149 (MQ=255)
                                                   gcCTCGAATGCTTCCATCTGCTCGCCCAGCGACTGCCCGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTc                                                                                                                                        <  3:21571/107‑1 (MQ=255)
                                                   gcCTCGAATGCTTCCATCTGCTCGCCCAGCGACTGCCCGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTc                                                                                                                                        >  4:21571/1‑107 (MQ=255)
                                                          aTGCTTCCATCTGCTCGCCCAGCGACTGCCCGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTGCCAGGCCAGTCAtgt                                                                                                               <  2:123729/125‑1 (MQ=255)
                                                              ttCCATCTGCTCGCCCAGCGACTGCCCGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTGCCAGGCCAGTCATGTGACAGCAGCTGCGCCAGCTGTGCAccc                                                                                    >  4:252940/1‑148 (MQ=255)
                                                                 cATCTGCTCGCCCAGCGACTGCCCGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTcacg                                                                                                                                     >  7:133176/1‑96 (MQ=255)
                                                                 cATCTGCTCGCCCAGCGACTGCCCGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTcacg                                                                                                                                     <  8:133176/96‑1 (MQ=255)
                                                                 cATCTGCTCGCCCAGCGACTGCCCGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTGCCAGGCCAGTCATGTGACAGCAGCTGCGCCAGCTGTGCACCCGATa                                                                                <  3:252940/149‑1 (MQ=255)
                                                                   tCTGCTCGCCCAGCGACTGCCCGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTGCCAGGCCAGTCATGTGACAGCAGCTGCGCCAGCTGTGCACCCGATAgc                                                                              >  3:123423/1‑149 (MQ=255)
                                                                       cTCGCCCAGCGACTGCCCGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCCCGTTGCCAGGCCAGTCATGTGACAGCAGCTGCGCCAGCTGTGCACCCGATAgcggcg                                                                          >  6:194876/1‑149 (MQ=255)
                                                                       cTCGCCCAGCGACTGCCCGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGtt                                                                                                                               <  1:22679/96‑1 (MQ=255)
                                                                       cTCGCCCAGCGACTGCCCGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGtt                                                                                                                               >  2:22679/1‑96 (MQ=255)
                                                                       cTCGCCCAGCGACTGCCCGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTGCCAGGCCAGTCATGTGACAGCAGCTGCGCCAGCTGTGCACCCGATAgcggcg                                                                          >  5:346217/1‑149 (MQ=255)
                                                                        tCGCCCAGCGACTGCCCGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTGCCAGGCCAGTCATGTGAc                                                                                                            >  8:179241/1‑114 (MQ=255)
                                                                        tCGCCCAGCGACTGCCCGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTGCCAGGCCAGTCATGTGAc                                                                                                            <  7:179241/114‑1 (MQ=255)
                                                                          gCCCAGCGACTGCCCGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTGCCAGGCCAGTCATGTGACAGCAGCTGCGCCAGCTGTGCACCCGATAGcggcgg                                                                         <  8:35506/147‑1 (MQ=255)
                                                                          gCCCAGCGACTGCCCGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTGCCAGGCCAGTCATGTGACAGCAGCTGCGCCAGCTGTGCACCCGATAGcggcgg                                                                         <  5:8410/147‑1 (MQ=255)
                                                                            ccAGCGACTGCCCGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTGCCAGGCCAGTCATGTGAcagc                                                                                                         >  1:309448/1‑113 (MQ=255)
                                                                            ccAGCGACTGCCCGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTGCCAGGCCAGTCATGTGAcagc                                                                                                         <  2:309448/113‑1 (MQ=255)
                                                                            ccAGCGACTGCCCGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTGCCAGGCCAGTCATGTGACAGCAGCTGCGCCAGCTGTGCACCCGATAGCGGCGGGGcc                                                                     >  3:292717/1‑149 (MQ=255)
                                                                            ccAGCGACTGCCCGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTGCCAGGCAAGTCATGTGACAGCAGATGCGCCAGATGTGCACCCCAAAGCGGCGGGGcc                                                                     >  2:206828/1‑149 (MQ=255)
                                                                             cAGCGACTGCCCGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTGCCAGGCCAGTCATGTGACAGCAGCTGCGCCAGCtgt                                                                                          <  8:230325/127‑1 (MQ=255)
                                                                             cAGCGACTGCCCGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTGCCAGGCCAGTCATGTGACAGCAGCTGCGCCAGCtgt                                                                                          >  7:230325/1‑127 (MQ=255)
                                                                             cAGCGACTGCCCGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTGCCAGGCCAGTCATGTGACAGCAGCTGCGCCAGCTGTGCACCCGATAGCGGCggg                                                                        <  8:295548/145‑1 (MQ=255)
                                                                             cAGCGACTGCCCGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTGCCAGGCCAGTCATGTGACAGCAGCTGCGCCAGCTGTGCACCCGATAGCGGCggg                                                                        >  7:295548/1‑145 (MQ=255)
                                                                                        cGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTGCCAGGCCAGTCATGTGACAGCAGCTGCGCCAGCTGTGCACCCGATAGCGGCGGGGCCGCGCGACCAAGc                                                         <  4:123423/149‑1 (MQ=255)
                                                                                         ggCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTGCCAGGCCAGTCATGTGACAGCAGCTGCGCCAGCTGTGCACCCGATAGCGGCGGGGCCGCGCGACCAAGCt                                                        <  5:194876/149‑1 (MQ=255)
                                                                                          gCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTGCCAGGCCAGTCATGTGACAGCAGCTGCGCCAGCTGTGCACCCGATAGCGGCGGGGCCGCGCGACCAAGCtt                                                       <  7:229920/149‑1 (MQ=255)
                                                                                          gCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTGCCAGGCCAGTCATGTGACAGCAGCTGCGCCAGCTGTGCACCCGATAGCGGCGGGGCCGCGCGACCAAGCtt                                                       <  7:10057/149‑1 (MQ=255)
                                                                                                        gATATTTGGCGAACCCAGTCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTGCCAGGCCAGTCATGTGACAGCAGCTGCGCCAGCTGTGCACCCGATAGCGGCGGGGCCGCGCGACCAAGCTTTTCACCGGCAGCAc                                         >  6:241734/1‑149 (MQ=255)
                                                                                                            tttGGCGAACCCAGTCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTGCCAGGCCAGTCATGTGACAGCAGCTGCGCCAGCTGTGCACCCGATAGCGGCGGGGCCGCGCGACCAAGCTTTTCACCGGCAGCACGGGc                                     <  5:241734/149‑1 (MQ=255)
                                                                                                                gCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTGCCATGCCAGTCATGTGACAGCAGCTGCGCCAGCTGTGCACCCGATAGCGGCGGGGCCGCGTGACCAAGCTTTTCACCGGCAGCACGGGCaaa                                  <  1:206828/148‑1 (MQ=255)
                                                                                                                gCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTGCCAGGCCAGTCATGTGACAGCAGCTGCGCCAGCTGTGCACCCGATAGCGGCGGGGCCGCGCGACCAAGCTTTTCACCGGCAGCACGGGCAAAg                                 >  1:326154/1‑149 (MQ=255)
                                                                                                                       cAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTGCCAGGCCAGTCATGTGACAGCAGCTGCGCCAGCTGTGCACCCGATAGCGGCGGGGCCGCGCGACCAAGCTTTTCACCGGCAGCACGGGCAAAGTGTTCGa                          <  6:346217/149‑1 (MQ=255)
                                                                                                                            ccAACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTGCCAGGCCAGTCATGTGACAGCAGCTGCGCCAGCTGTGCACCCGATAGCGGCGGGGCCGCGCGACCAAGCTTTTCACCGGCAGCACGGGCAAAGTGTTCGAAcag                      >  4:46622/1‑148 (MQ=255)
                                                                                                                               aCGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTGCCAGGCCAGTCATGTGACAGCAGCTGCGCCAGCTGTGCACCCGATAGCGGCGGGGCCGCGCGACCAAGCTTTTCACCGGCAGCACGGGCAAAGTGTTCGAACagcagc                  <  2:326154/149‑1 (MQ=255)
                                                                                                                                   aTGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTGCCAGGCCAGTCATGTGACAGCAGCTGCGCCAGCTGTGCACCCGATAGCGGCGGGGCCGCGCGa                                                               <  1:281243/100‑1 (MQ=255)
                                                                                                                                   aTGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTGCCAGGCCAGTCATGTGACAGCAGCTGCGCCAGCTGTGCACCCGATAGCGGCGGGGCCGCGCGa                                                               >  2:281243/1‑100 (MQ=255)
                                                                                                                                    tGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTGCCAGGCCAGTCATGTGACAGCAGCTGCGCCAGCTGTGCACCCGATAGCGGCGGGGCCGCGCGACCAAGCTTTTCACCGGCAGCACGGGCAAAGTGTTCGAACAGCAGCGGAAt             <  4:292717/149‑1 (MQ=255)
                                                                                                                                      ccGCTCCGCCACATTGGCCAGTTCACGCACGTTGCCAGGCCAGTCATGTGACAGCAGCTGCGCCAGCTGTGCACCCGATAGCGGCGGGGCCGCGCGACCAAGCTTTTCACCGGCAGCACGGGCAAAGTGTTc                            >  4:80696/1‑132 (MQ=255)
                                                                                                                                      ccGCTCCGCCACATTGGCCAGTTCACGCACGTTGCCAGGCCAGTCATGTGACAGCAGCTGCGCCAGCTGTGCACCCGATAGCGGCGGGGCCGCGCGACCAAGCTTTTCACCGGCAGCACGGGCAAAGTGTTc                            <  3:80696/132‑1 (MQ=255)
                                                                                                                                      ccGCTCCGCCACATTGGCCAGTTCACGCACGTTGCCAGGCCAGTCATGTGACAGCAGCTGCGCCAGCTGTGCACCCGATAGCGGCGGGGCCGCGCGACCAAGCTTTTCACCGGCAGCACGGGCAAAGTGTTc                            <  3:85536/132‑1 (MQ=255)
                                                                                                                                      ccGCTCCGCCACATTGGCCAGTTCACGCACGTTGCCAGGCCAGTCATGTGACAGCAGCTGCGCCAGCTGTGCACCCGATAGCGGCGGGGCCGCGCGACCAAGCTTTTCACCGGCAGCACGGGCAAAGTGTTc                            >  4:85536/1‑132 (MQ=255)
                                                                                                                                               cgcATTGGCCAGTTCACGCACGTTGCCAGGCCAGTCATGTGACAGCAGCTGCGCCAGCTGTGCACCCGATAGCGGCGGGGCCGCGCGACCAAGCTTTTCACCGGCAGCACGGGCAAAGTGTTCGAACAGCAGCGGAATGTCCTCccgcc  <  3:46622/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
ACCGACTTGATCTCGCCCCCATGCTGACGCAAGGCCGCACGCAGGCATTGCGCCTCGAATGCTTCCATCTGCTCGCCCAGCGACTGCCCGGCAGGTGCCACCTCGATATTTGGCGAACCCAGCCCCAACGCATGCCGCTCCGCCGCATTGGCCAGTTCACGCACGTTGCCAGGCCAGTCATGTGACAGCAGCTGCGCCAGCTGTGCACCCGATAGCGGCGGGGCCGCGCGACCAAGCTTTTCACCGGCAGCACGGGCAAAGTGTTCGAACAGCAGCGGAATGTCCTCCCGCC  >  NC_002947/1595767‑1596058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑