Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,642,246	T→C	7.9%	intergenic (‑74/+78)	PP_1442 ← / ← lon‑I	hypothetical protein/DNA‑binding ATP‑dependent protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,642,246	0	T	C	7.9%	211.2 / 7.1	76	intergenic (‑74/+78)	PP_1442/lon‑I	hypothetical protein/DNA‑binding ATP‑dependent protease
Reads supporting (aligned to +/- strand):  ref base T (35/35);  new base C (3/3);  total (38/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01

CGAGGATTGTTGCGCGCAAGCAGAAAGCAGGGCGAAGCTCAGGGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGC‑TTGGCGAAATGCACTGTCAGGCCTTCGCGCAGG  >  NC_002947/1642104‑1642390                                                                                                                                               |                                                                                                                                                  cGAGGATTGTTGCGCGCAAGCAGAAAGCAGGGCGAAGCTCAGGGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTcgc                                                                                                                                              >  7:172551/1‑148 (MQ=255)   aGGATTGTTGCGCGCAAGCAGAAAGCAGGGCGAAGCTCAGGGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAg                                                                                                                                           <  2:52925/149‑1 (MQ=255)    ggATTGTTGCGCGCAAGCAGAAAGCAGGGCGAAGCTCAGGGGAACGAGCAGTCGAGGAGAGGTGATGGTGGCTCCGAAGGCTGGCGCTGGCCTGAGGATACCCGATGATGGAGGAAATTTGTGCTGCCTGTGCCGGTACTTTAGCGAg                                                                                                                                           >  1:87967/1‑148 (MQ=255)      aTTGTTGCGCGCAAGCAGAAAGCAGGGCGAAGCTCAGGGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTaa                                                                                                                                        <  8:172551/149‑1 (MQ=255)              gcgcAAGCAGAAAGCAGGGCGAAGCTCAGGGGAACGAGCAGCCGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCgcgc                                                                                                                                >  5:159390/1‑149 (MQ=255)                  aaGCAGAAAGCAGGGCGAAGCTCAGGGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGTTCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGa                                                                                                                                            <  6:259055/133‑1 (MQ=255)                  aaGCAGAAAGCAGGGCGAAGCTCAGGGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGa                                                                                                                                            >  5:259055/1‑133 (MQ=255)                      aGAAAGCAGGGCGAAGCTCAGGGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAggg                                                                                                                        >  3:193160/1‑149 (MQ=255)                      aGAAAGCAGGGCGAAGCTCAGGGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGCACCGCCCTCTTCGCGGGCAAGCCCGCACCTGCAcgg                                                                                                                        >  5:167727/1‑149 (MQ=37)                      aGAAAGCAGGGCGAAGCTCAGGGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGCACCGCCCTCTTCGCGGGCAAGCCCGCACCTGCAcgg                                                                                                                        >  5:162593/1‑149 (MQ=37)                       gAAAGCAGGGCGAAGCTCAGGGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCAGCCTGCACCGCCCTCTTCGCGGGCAAGCCCGCACCTGCAcggg                                                                                                                       >  3:217977/1‑149 (MQ=37)                        aaaGCAGGGCGAAGCTCAGGGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAgggg                                                                                                                       >  2:87933/1‑148 (MQ=255)                          aGCAGGGCGAAGCTCAGGGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCgcgc                                                                                                                                >  4:223171/1‑137 (MQ=255)                          aGCAGGGCGAAGCTCAGGGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCgcgc                                                                                                                                <  3:223171/137‑1 (MQ=255)                           gCAGGGCGAAGCTCAGGGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTcgc                                                                                                                                              <  5:193322/122‑1 (MQ=255)                           gCAGGGCGAAGCTCAGGGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTcgc                                                                                                                                              >  6:193322/1‑122 (MQ=255)                             aGGGCGAAGCTCAGGGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCgcgc                                                                                                                                >  5:80921/1‑134 (MQ=255)                             aGGGCGAAGCTCAGGGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCgcgc                                                                                                                                <  6:80921/134‑1 (MQ=255)                                     gCTCAGGGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCCTTCGCGGGCAAGCCCGCACCTGCACGGGTACGCTACCCCTGc                                                                                                         <  5:220988/149‑1 (MQ=37)                                      cTCAGGGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGCACCGCCCTCTTCGCGGGCAAGCCCGCACCTGCACGGGTACGCTACCCCTGCa                                                                                                        <  6:162593/149‑1 (MQ=21)                                      cTCAGGGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGCACCGCCCTCTTCGCGGGCAAGCCCGCACCTGCACGGGTACGCTACCCCTGCa                                                                                                        <  6:167727/149‑1 (MQ=21)                                       tCAGGGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAg                                                                                                       >  2:75230/1‑149 (MQ=255)                                        cAGGGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAgg                                                                                                      >  1:63470/1‑149 (MQ=255)                                           gggAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGTGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGc                                                                                                   >  8:294077/1‑149 (MQ=255)                                           gggAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGc                                                                                                   >  4:180860/1‑149 (MQ=255)                                            ggAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCg                                                                                                  <  2:63470/149‑1 (MQ=255)                                             gAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCgg                                                                                                 >  8:186084/1‑149 (MQ=255)                                              aaCGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGCTGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCggg                                                                                                <  7:186084/149‑1 (MQ=255)                                               aCGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGc                                                                                               <  2:336044/149‑1 (MQ=255)                                                   gCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCACGGGTACGCTACCCCTGCAGgcgc                                                                                                   <  7:135036/141‑1 (MQ=38)                                                   gCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCACGGGTACGCTACCCCTGCAGgcgc                                                                                                   >  8:135036/1‑141 (MQ=38)                                                         gaggagAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCtt                                                                                             <  3:196308/141‑1 (MQ=255)                                                         gaggagAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCtt                                                                                             >  4:196308/1‑141 (MQ=255)                                                           ggagAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCTTGCCcgcg                                                                                      >  8:312158/1‑146 (MQ=255)                                                                         ggCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGc                                                                     <  3:180860/149‑1 (MQ=255)                                                                          gCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGt                                                                                                     <  8:257244/116‑1 (MQ=255)                                                                          gCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGt                                                                                                     >  7:257244/1‑116 (MQ=255)                                                                          gCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGc                                                                     >  2:281027/1‑148 (MQ=255)                                                                           cTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTa                                                                   <  1:75230/149‑1 (MQ=255)                                                                              cGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGaa                                                                >  7:293425/1‑149 (MQ=255)                                                                              cGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACACGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGa                                                                 >  2:213628/1‑148 (MQ=255)                                                                                  ggCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGTGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCg                                                                             <  1:278079/132‑1 (MQ=255)                                                                                  ggCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCg                                                                             >  2:278079/1‑132 (MQ=255)                                                                                        cGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTc                                                      <  1:281027/149‑1 (MQ=255)                                                                                        cGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTc                                                      >  3:72194/1‑149 (MQ=255)                                                                                         gATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGt                                                                                                                      >  5:153784/1‑84 (MQ=255)                                                                                         gATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGt                                                                                                                      <  6:153784/84‑1 (MQ=255)                                                                                                  aGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCg                                             <  8:293425/148‑1 (MQ=255)                                                                                                  aGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCg                                             <  6:159390/148‑1 (MQ=255)                                                                                                       aCCCGGTGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCa                                                                        >  7:320424/1‑116 (MQ=255)                                                                                                       aCCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGt                                                                           >  4:267752/1‑113 (MQ=255)                                                                                                       aCCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGt                                                                           <  3:267752/113‑1 (MQ=255)                                                                                                       aCCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCa                                                                        <  8:320424/116‑1 (MQ=255)                                                                                                         ccGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACa                                                              >  4:51625/1‑124 (MQ=255)                                                                                                         ccGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACa                                                              <  3:51625/124‑1 (MQ=255)                                                                                                           gatgatCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAg                                                                  >  5:190103/1‑118 (MQ=255)                                                                                                           gatgatCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAg                                                                  <  6:190103/118‑1 (MQ=255)                                                                                                               atCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGC‑tt                                 >  6:332707/1‑146 (MQ=255)                                                                                                               atCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGC‑tt                                 <  5:332707/146‑1 (MQ=255)                                                                                                                tCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGC‑TTGGc                              >  8:40847/1‑148 (MQ=255)                                                                                                                tCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGC‑TTGGc                              >  8:35434/1‑148 (MQ=255)                                                                                                                  gAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGC‑TTGGCGa                            >  3:116390/1‑148 (MQ=255)                                                                                                                   aGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGc                                                                     >  6:332673/1‑107 (MQ=255)                                                                                                                   aGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGc                                                                     <  5:332673/107‑1 (MQ=255)                                                                                                                      aaaTTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGC‑TTGGCGAAATGc                       <  7:312158/149‑1 (MQ=255)                                                                                                                      aaaTTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGC‑TTGGCGAAATGc                       <  7:35434/149‑1 (MQ=255)                                                                                                                      aaaTTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGC‑TTGGCGAAATGc                       <  7:40847/149‑1 (MQ=255)                                                                                                                             tgctgcCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGC‑TTGGCGAAATGCACTGTCa                <  7:294077/149‑1 (MQ=255)                                                                                                                               ctgcCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGC‑TTGGCGAAATGCACTGTCAgg              <  4:193160/149‑1 (MQ=255)                                                                                                                                 gcCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGC‑TTGGCGAAATGCACTGTCAGGcc            >  3:293886/1‑149 (MQ=255)                                                                                                                                  ccTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGC‑TTGGCGAAATGCACTGTCAGGc             <  2:87967/147‑1 (MQ=255)                                                                                                                                      tgCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGC‑TTGGCGAAATGCACTGTCAGGCCTTcg        <  1:87933/148‑1 (MQ=255)                                                                                                                                         cGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGC‑TTGGCGAAATGCACTGTCAg               >  8:177410/1‑138 (MQ=255)                                                                                                                                         cGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGC‑TTGGCGAAATGCACTGTCAg               <  7:177410/138‑1 (MQ=255)                                                                                                                                         cGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGC‑TTGGCGAAATGCACTGTCAGGCCTTCGCGCa    <  4:116390/149‑1 (MQ=255)                                                                                                                                            tCCTTTCGCGAGTAAGCCCGGTCATGCAGGGGTAGCGTACCCGTGCAGGTTCGGGCTTGCCCGCGAAGATGGAGGTGCAGTCTATAAAATCACTTTCGCCACGTCGGCAAAGCGCTTTGGCGAAATGCACTGTCAGGCCTTCGCGCAgg  <  1:213628/149‑1 (MQ=255)                                                                                                                                               tttCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGC‑TTGGCGAAATGCa                      >  4:175859/1‑125 (MQ=255)                                                                                                                                               tttCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGC‑TTGGCGAAATGCa                      <  3:175859/125‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                  CGAGGATTGTTGCGCGCAAGCAGAAAGCAGGGCGAAGCTCAGGGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCGTACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGC‑TTGGCGAAATGCACTGTCAGGCCTTCGCGCAGG  >  NC_002947/1642104‑1642390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑