Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,667,902 A→G 8.1% S302G (AGC→GGC)  ffh → signal recognition particle GTPase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,667,9020AG 8.1% 134.7 / 2.8 50S302G (AGC→GGC) ffhsignal recognition particle GTPase subunit
Reads supporting (aligned to +/- strand):  ref base A (23/22);  new base G (2/2);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.45e-01

GCCCGAGGCGGTGCTGCGCTGTCGGTACGTGCAATCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAG‑GGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCGGCGGCCT  >  NC_002947/1667758‑1668047
                                                                                                                                                |                                                                                                                                                  
gCCCGAGGCGGTGCTGCGCTGTCGGTACGTGCAATCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGcc                                                                                                                                                 <  5:156016/148‑1 (MQ=255)
gCCCGAGGCGGTGCTGCGCTGTCGGTACGTGCAATCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGcc                                                                                                                                                 >  6:156016/1‑148 (MQ=255)
   cGAGGCGGTGCTGCGCTGTCGGTACGTGCAATCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGAt                                                                                                                                             >  4:160106/1‑149 (MQ=255)
               gcgcTGTCGGTACGTGCAATCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGcc                                                                                                                                   >  6:327437/1‑147 (MQ=255)
                        gTACGTGCAATCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCAt                                                                                                                        <  5:327437/149‑1 (MQ=255)
                         tACGTGCAATCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATc                                                                                                                       <  3:243469/149‑1 (MQ=255)
                            gTGCAATCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATc                                                                                                                                            <  3:118960/125‑1 (MQ=255)
                            gTGCAATCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATc                                                                                                                                            >  4:118960/1‑125 (MQ=255)
                            gTGCAATCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAg                                                                                                                                <  5:85102/137‑1 (MQ=255)
                            gTGCAATCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAg                                                                                                                                >  6:85102/1‑137 (MQ=255)
                             tGCAATCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGAc                                                                                                                    >  2:144984/1‑148 (MQ=255)
                                 aTCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGAcaaggc                                                                                                               <  7:116026/149‑1 (MQ=255)
                                  tCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGAcaaggc                                                                                                               >  1:181153/1‑148 (MQ=255)
                                        gCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGAcaag                                                                                                                 <  1:284855/140‑1 (MQ=255)
                                        gCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGAcaag                                                                                                                 >  2:284855/1‑140 (MQ=255)
                                           taCCGATGAACTTGATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGAACGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGa                                                                                                      >  2:79029/2‑148 (MQ=255)
                                                 tCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCa                                                                                                                                       >  4:177958/1‑109 (MQ=255)
                                                 tCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCa                                                                                                                                       <  3:177958/109‑1 (MQ=255)
                                                  cAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCa                                                                                                                                       >  6:337990/1‑108 (MQ=255)
                                                   aaGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTgg                                                                                              <  2:181153/148‑1 (MQ=255)
                                                     gTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCGGCCTGCTCGATCAGGCTGAGc                                                                                                                               <  4:82474/113‑1 (MQ=255)
                                                         aTCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGaa                                                                                       >  4:230119/1‑149 (MQ=255)
                                                                tGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTgaag                                                                                <  3:160106/149‑1 (MQ=255)
                                                                tGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTg                                                                                   >  5:286566/1‑146 (MQ=255)
                                                                  ggTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTgaagaa                                                                              <  1:144984/149‑1 (MQ=255)
                                                                      agaagaCCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGaag‑ggc                                                                         <  2:201302/149‑1 (MQ=255)
                                                                       gaagaCCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCGGCCTGCTCGATCAGGCTGAGCacg                                                                                                                            <  4:250016/98‑3 (MQ=38)
                                                                       gaagaCCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCGGCCTGCTCGATCAGGCTGAGCacg                                                                                                                            >  3:250016/1‑96 (MQ=38)
                                                                             cGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGaag‑ggcaag                                                                      >  5:355184/1‑145 (MQ=255)
                                                                             cGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGaag‑ggcaag                                                                      <  6:355184/145‑1 (MQ=255)
                                                                                    cTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAG‑GGCAAGGGCTTCGACCt                                                           >  2:277829/1‑149 (MQ=255)
                                                                                     tCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGaaa                                                                                      <  8:156243/122‑1 (MQ=255)
                                                                                     tCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGaaa                                                                                      >  7:156243/1‑122 (MQ=255)
                                                                                               ccACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAG‑GGCAAGGGCTTCGACCTCGAAGACTTc                                                 <  1:32247/148‑1 (MQ=255)
                                                                                               ccACCCCGACCGCCTCGCTTCGCGCATCCGCGGCATGGGCGACGTGCTCCGCCTGATCGAGCAGGACGAGCAGACCATCGACAAGTCCAAGGCCGACAACCTGGCCAAGAAACTGAAGAAG‑GGCAAGGGCTTCGACCTCGAAGACTTc                                                 <  2:43855/148‑1 (MQ=255)
                                                                                                 aCCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAG‑GGCAAGGGCTTCGACCTCGAAGACTTCcgc                                              <  6:286566/149‑1 (MQ=255)
                                                                                                  ccccGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGc                                                                                             <  2:332225/102‑1 (MQ=255)
                                                                                                     cGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAG‑GGCAAGGGCTTCGACCTCGAAGACTTCCGCGAc                                           <  8:278977/148‑1 (MQ=255)
                                                                                                     cGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAG‑GGCAAGGGCTTCGACCTCGAAGACTTCCGCGAc                                           >  7:278977/1‑148 (MQ=255)
                                                                                                                   cgcgCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGTGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCaaa                              <  1:79029/148‑1 (MQ=255)
                                                                                                                    gcgcATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGTCCAAGAAACTGAAGAAG‑GGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATga                           <  1:277829/149‑1 (MQ=255)
                                                                                                                    gcgcATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAG‑GGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATg                            >  2:179769/1‑148 (MQ=255)
                                                                                                                            tCGGCGTGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAG‑GGCAAGGGCTTCGACCTCGaa                                                       >  8:267520/1‑113 (MQ=255)
                                                                                                                            tCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCTAGAAACTGAAGAAG‑GGCAAGGGCTTCGACCTCGAAGAc                                                    >  1:109592/1‑116 (MQ=255)
                                                                                                                            tCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAG‑GGCAAGGGCTTCGACCTCGAAGAc                                                    <  2:109592/116‑1 (MQ=255)
                                                                                                                                     gCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAG‑GGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTc          >  8:78018/1‑149 (MQ=255)
                                                                                                                                         tctGCTCGGCCTGCTCGATCAGGCTGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAG‑GGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTcggcg      >  7:106493/3‑149 (MQ=255)
                                                                                                                                             cTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACTAGCTGGCCAAGAAACTGAAGAAG‑GGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCGGCGGCCt  >  6:301176/1‑149 (MQ=255)
                                                                                                                                              tCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAG‑GGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCggcggc    >  6:64244/1‑146 (MQ=255)
                                                                                                                                              tCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAG‑GGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCggcggc    <  5:64244/146‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
GCCCGAGGCGGTGCTGCGCTGTCGGTACGTGCAATCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAG‑GGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCGGCGGCCT  >  NC_002947/1667758‑1668047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑