Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,685,783 G→C 5.4% A332A (GCC→GCG)  patD ← medium chain aldehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,685,7830GC 5.4% 235.4 / 2.6 73A332A (GCC→GCG) patDmedium chain aldehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (30/39);  new base C (2/2);  total (32/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAAACACTTCGTTGCGCACGATCTCGTCGTCCTGCAGGGCGTCGGCCAGCACGGTAGGCTCGAAGAAAAAGCCATCGCCCGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTGCACATACAGGCGGCAG  >  NC_002947/1685637‑1685930
                                                                                                                                                  |                                                                                                                                                   
ccAAACACTTCGTTGCGCACGATCTCGTCGTCCTGCAGGGCGTCGGCCAGCACGGTAGGCTCGAAGAAAAAGCCATCGCCCGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGc                                                                                                                                                   <  3:288379/149‑1 (MQ=255)
  aaaCACTTCGTTGCGCACGATCTCGTCGTCCTGCAGGGCGTCGGCCAGCATGGTAGGCTCGAAGAAAAAGCCATCGCCCGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGa                                                                                                                                                 >  5:191150/1‑149 (MQ=255)
        ttCGTTGCGCACGATCTCGTCGTCCTGCAGGGCGTCGGCCAGCACGGTAGGCTCGAAGAAAAAGCCATCGCCCGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGcccagct                                                                                                                                           >  1:286862/1‑145 (MQ=255)
        ttCGTTGCGCACGATCTCGTCGTCCTGCAGGGCGTCGGCCAGCACGGTAGGCTCGAAGAAAAAGCCATCGCCCGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGAc                                                                                                                                                <  2:286862/144‑1 (MQ=255)
          cGTTGCGCACGATCTCGTCGTCCTGCAGGGCGTCGGCCAGCACGGTAGGCTCGAAGAAAAAGCCATCGCCCGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCGGCCCGGCGACCTTGt                                                                                                                                           <  5:324608/147‑1 (MQ=255)
            ttGCGCACGATCTCGTCGTCCTGCAGGGCGTCGGCCAGCACGGTAGGCTCGAAGAAAAAGCCATCGCCCGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTcgcg                                                                                                                                       <  5:208115/149‑1 (MQ=255)
            gtGCTCACGATCTCGTCGTCCTGCAGGGCGTCGGCGAGGACGGTAGGCTCGAAGAAAACGCCATGGCGCGCTATTGGCTTGGCAGCGGTTAGCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCTCCAGCCCGGCGACCTTTTcggg                                                                                                                                       <  8:144894/148‑3 (MQ=255)
              gcgcACGATCTCGTCGTCCTGCAGGGCGTCGGCCAGCACGGTAGGCTCGAAGAAAAAGCCATCGCCCGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCgg                                                                                                                                      <  6:333354/148‑1 (MQ=255)
                 cACGATCTCGTCGTCCTGCAGGGCGTCGGCCAGCACGGTAGGCTCGAAGAAAAAGCCATCGCCCGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCt                                                                                                                                  >  4:295229/1‑149 (MQ=255)
                          gtcgtcCTGCAGGGCGTCGGCCAGCACGGTAGGCTCGAAGAAAAAGCCATCGCCCGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGa                                                                                                                         >  7:81743/1‑149 (MQ=255)
                           tcgtcCTGCAGGGCGTCGGCCAGCACGGTAGGCTCGAAGAAAAAGCCATCGCCCGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGAt                                                                                                                        >  5:362618/1‑149 (MQ=255)
                              tcCTGCAGGGCGTCGGCCAGCACGGTAGGCTCGAAGAAAAAGCCATCGCCCGCTATTGGCTTGCCACCGATTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAg                                                                                                                     >  8:192466/1‑149 (MQ=255)
                                cTGCAGGGCGTCGGCCAGCACGGTAGGCTCGAAGAAAAAGCCATCGCCCGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTg                                                                                                                   >  3:260074/1‑149 (MQ=255)
                                  gCAGGGCGTCGGCCAGCACGGTAGGCTCGAAGAAAAAGCCATCGCCCGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGc                                                                                                                 >  3:23481/1‑149 (MQ=255)
                                    aGGGCGTCGGCCAGCACGGTAGGCTCGAAGAAAAAGCCATCGCCCGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGccc                                                                                                               >  6:194552/1‑149 (MQ=255)
                                         gTCGGCCAGCACGGTAGGCTCGAAGAAAAAGCCATCGCCCGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGAc                                                                                                                                                >  8:161600/1‑111 (MQ=255)
                                         gTCGGCCAGCACGGTAGGCTCGAAGAAAAAGCCATCGCCCGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGAc                                                                                                                                                <  7:161600/111‑1 (MQ=255)
                                          tCGGCCAGCACGGTAGGCTCGAAGAAAAAGCCATCGCCCGCTATTGGCTTGCCACCGGTTACCAGACGAATCTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTc                                                                                                         <  1:112877/149‑1 (MQ=255)
                                            ggCCAGCACGGTAGGCTCGAAGAAAAAGCCATCGCCCGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCgg                                                                                                       <  3:212031/149‑1 (MQ=255)
                                             gCCAGCACGGTAGGCTCGAAGAAAAAGCCATCGCCCGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTgg                                                                                                                                <  6:88726/123‑1 (MQ=255)
                                             gCCAGCACGGTAGGCTCGAAGAAAAAGCCATCGCCCGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCAACAGCCCGGCGAACTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGt                                                                                                      <  1:103146/149‑1 (MQ=255)
                                              ccAGCACGGTAGGCTCGAAGAAAAAGCCATCGCCCGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCgg                                                                                                       <  6:106600/147‑1 (MQ=255)
                                              ccAGCACGGTAGGCTCGAAGAAAAAGCCATCGCCCGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGta                                                                                                     >  5:106600/1‑148 (MQ=255)
                                               cAGCACGGTAGGCTCGAAGAAAAAGCCATCGCCCGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGtt                                                                                                     >  8:179407/1‑148 (MQ=255)
                                                  cACGGTAGGCTCGAAGAAAAAGCCATCGCCCGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTttctt                                                                                                 <  7:179407/149‑1 (MQ=255)
                                                  cACGGTAGGCTCGAAGAAAAAGCCATCGCCCGCTATTGGCTTGCCACCGATTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTttctt                                                                                                 <  7:192466/149‑1 (MQ=255)
                                                   aCGGTAGGCTCGAAGAAAAAGCCATCGCCCGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTttcttc                                                                                                <  2:100898/149‑1 (MQ=255)
                                                    cGGTAGGCTCGAAGAAAAAGCCATCGCCCGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCa                                                                                               <  3:295229/149‑1 (MQ=255)
                                                    cGGTAGGCTCGAAGAAAAAGCCATCGCCCGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCa                                                                                               <  7:2478/149‑1 (MQ=255)
                                                     ggTAGGCTCGAAGAAAAAGCCATCGCCCGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTttctt                                                                                                 >  1:343942/1‑146 (MQ=255)
                                                           cTCGAAGAAAAAGCCATCGCCCGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCt                                                                                        <  6:362618/149‑1 (MQ=255)
                                                                   aaaaGCCATCGCCCGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTttc                                                                                                   >  4:312173/1‑130 (MQ=255)
                                                                   aaaaGCCATCGCCCGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTttc                                                                                                   <  3:312173/130‑1 (MQ=255)
                                                                    aaaGCCATCGCCCGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCg                                                                               >  5:68463/1‑149 (MQ=255)
                                                                        ccATCGCCCGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCtt                                                                           >  3:206786/1‑149 (MQ=255)
                                                                          aTCGCCCGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCTCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGa                                                                         <  4:260074/149‑1 (MQ=255)
                                                                          aTCGCCCGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCtt                                                                           >  1:300699/1‑147 (MQ=255)
                                                                          aTCGCCCGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGa                                                                         <  6:191150/149‑1 (MQ=255)
                                                                          aTCGCCCGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGa                                                                         <  5:194552/149‑1 (MQ=255)
                                                                              cccGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCa                                                                                               >  7:78405/1‑123 (MQ=255)
                                                                               ccGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAggctg                                                                    <  7:225559/149‑1 (MQ=255)
                                                                                cGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTttcttc                                                                                                <  4:34053/120‑1 (MQ=255)
                                                                                cGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTttcttc                                                                                                >  3:34053/1‑120 (MQ=255)
                                                                                   tATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGa                                                                         <  3:201545/140‑1 (MQ=255)
                                                                                   tATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTa                                                                >  4:194783/1‑149 (MQ=255)
                                                                                   tATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGACCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGa                                                                         >  4:201545/1‑140 (MQ=255)
                                                                                     ttGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTAc                                                               <  8:81743/148‑1 (MQ=255)
                                                                                     ttGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACg                                                              >  4:327282/1‑149 (MQ=255)
                                                                                        gCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCt                                                           <  3:249411/149‑1 (MQ=255)
                                                                                          ttGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCtt                                                          >  3:278480/1‑148 (MQ=255)
                                                                                             ccACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCg                                                                                                        >  8:116721/1‑99 (MQ=255)
                                                                                             ccACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCg                                                                                                        <  1:225834/99‑1 (MQ=255)
                                                                                             ccACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCg                                                                                                        <  7:116721/99‑1 (MQ=255)
                                                                                             ccACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCg                                                                                                        >  2:225834/1‑99 (MQ=255)
                                                                                               aCCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTg                                                                                       <  2:63948/114‑1 (MQ=255)
                                                                                               aCCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCa                                                    <  6:68463/149‑1 (MQ=255)
                                                                                               aCCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCa                                                    <  3:327282/149‑1 (MQ=255)
                                                                                                  ggTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGc                                                 >  4:7180/1‑149 (MQ=255)
                                                                                                       ccAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTc                                             <  2:343942/148‑1 (MQ=255)
                                                                                                        cAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGa                                           <  3:194783/149‑1 (MQ=255)
                                                                                                                             gATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGt                      >  1:114340/1‑149 (MQ=255)
                                                                                                                                 gCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCt                   <  2:300699/148‑1 (MQ=255)
                                                                                                                                  cccGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGGTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTg                  >  7:88186/1‑148 (MQ=255)
                                                                                                                                   ccGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTGca                >  5:138323/1‑149 (MQ=255)
                                                                                                                                       gcgcccAGCACCGCGACAAGGTCGCCGGGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGTTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTa                              >  4:339426/5‑131 (MQ=38)
                                                                                                                                       gcgcccAGCACCGCGACAAGGTCGCCGGGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGTTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTa                              <  3:344881/127‑1 (MQ=38)
                                                                                                                                       gcgcccAGCACCGCGACAAGGTCGCCGGGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGTTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTa                              >  4:344881/5‑131 (MQ=38)
                                                                                                                                       gcgcccAGCACCGCGACAAGGTCGCCGGGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGTTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTa                              <  3:339426/127‑1 (MQ=38)
                                                                                                                                         cACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTGCACATACa          <  4:206786/149‑1 (MQ=255)
                                                                                                                                          aCCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTGCACATACAg         <  6:138323/149‑1 (MQ=255)
                                                                                                                                          aCCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTGCACATACAg         <  4:278480/149‑1 (MQ=255)
                                                                                                                                           cgaGCCCGGCGACCTTGTGGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGAc                                          <  1:102354/113‑1 (MQ=255)
                                                                                                                                                 cGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACACAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTGCACATACAGGCGGCAg  >  6:118518/1‑149 (MQ=255)
                                                                                                                                                  ggCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAg                                      <  3:243037/112‑1 (MQ=255)
                                                                                                                                                  ggCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAg                                      >  4:243037/1‑112 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CCAAACACTTCGTTGCGCACGATCTCGTCGTCCTGCAGGGCGTCGGCCAGCACGGTAGGCTCGAAGAAAAAGCCATCGCCCGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTGCACATACAGGCGGCAG  >  NC_002947/1685637‑1685930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑