Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,685,862 C→T 7.8% S306N (AGC→AAC)  patD ← medium chain aldehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,685,8620CT 7.8% 148.0 / 2.1 51S306N (AGC→AAC) patDmedium chain aldehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (26/21);  new base T (2/2);  total (28/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.69e-01

CCGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTGCACATACAGGCGGCAGGCTGCGGTGCAGTCCTGGCCGGCGTTGTAGAAGCCGAAGGTGCGGATGCCGTCGATGGCGGCATCGATGTCAGCGTCATC  >  NC_002947/1685716‑1686010
                                                                                                                                                  |                                                                                                                                                    
ccGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAggctg                                                                                                                                                    <  7:225559/149‑1 (MQ=255)
    tATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTa                                                                                                                                                >  4:194783/1‑149 (MQ=255)
      ttGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTAc                                                                                                                                               <  8:81743/148‑1 (MQ=255)
      ttGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACg                                                                                                                                              >  4:327282/1‑149 (MQ=255)
         gCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCt                                                                                                                                           <  3:249411/149‑1 (MQ=255)
           ttGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCtt                                                                                                                                          >  3:278480/1‑148 (MQ=255)
                aCCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCa                                                                                                                                    <  3:327282/149‑1 (MQ=255)
                aCCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCa                                                                                                                                    <  6:68463/149‑1 (MQ=255)
                   ggTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGc                                                                                                                                 >  4:7180/1‑149 (MQ=255)
                        ccAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTc                                                                                                                             <  2:343942/148‑1 (MQ=255)
                         cAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGa                                                                                                                           <  3:194783/149‑1 (MQ=255)
                                              gATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGt                                                                                                      >  1:114340/1‑149 (MQ=255)
                                                  gCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCt                                                                                                   <  2:300699/148‑1 (MQ=255)
                                                   cccGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGGTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTg                                                                                                  >  7:88186/1‑148 (MQ=255)
                                                    ccGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTGca                                                                                                >  5:138323/1‑149 (MQ=255)
                                                        gcgcccAGCACCGCGACAAGGTCGCCGGGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGTTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTa                                                                                                              >  4:339426/5‑131 (MQ=38)
                                                        gcgcccAGCACCGCGACAAGGTCGCCGGGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGTTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTa                                                                                                              >  4:344881/5‑131 (MQ=38)
                                                        gcgcccAGCACCGCGACAAGGTCGCCGGGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGTTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTa                                                                                                              <  3:344881/127‑1 (MQ=38)
                                                        gcgcccAGCACCGCGACAAGGTCGCCGGGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGTTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTa                                                                                                              <  3:339426/127‑1 (MQ=38)
                                                          cACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTGCACATACa                                                                                          <  4:206786/149‑1 (MQ=255)
                                                           aCCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTGCACATACAg                                                                                         <  6:138323/149‑1 (MQ=255)
                                                           aCCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTGCACATACAg                                                                                         <  4:278480/149‑1 (MQ=255)
                                                            cgaGCCCGGCGACCTTGTGGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGAc                                                                                                                          <  1:102354/113‑1 (MQ=255)
                                                                  cGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACACAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTGCACATACAGGCGGCAg                                                                                  >  6:118518/1‑149 (MQ=255)
                                                                   ggCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAg                                                                                                                      >  4:243037/1‑112 (MQ=255)
                                                                   ggCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAg                                                                                                                      <  3:243037/112‑1 (MQ=255)
                                                                    gCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTGCACATACAGGCGGCAGGc                                                                                >  6:258764/1‑149 (MQ=255)
                                                                      gACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTGCACATACAGGCGGCAGCCTg                                                                              >  5:109214/1‑149 (MQ=255)
                                                                            gTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTGCACATACAGGCGGCAGGCTGCGGTg                                                                         >  5:48842/1‑148 (MQ=255)
                                                                             tCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTGCACATACAGGCGGCAGGCTGCGGTGCa                                                                       <  3:7180/149‑1 (MQ=255)
                                                                               gcgGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTGCACATACAGGCGGCAGGCTGCGGTGCAGt                                                                     >  3:19207/1‑149 (MQ=255)
                                                                                           gcTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCACGCGTTCTACAAAGCGCTCGTAGATCCCGTCCTGAACATACATGCGGAAGGCTGCGCTg                                                                         >  6:312591/1‑133 (MQ=255)
                                                                                               aTCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTGCACATACAGGCGGCAGGCTGCGGTGCAGTCCTGGCCGGCGTTg                                                       >  6:289726/1‑147 (MQ=255)
                                                                                                  aGTGGCCCCAGTTCGGGTTCTTCAGACTCCTGCAGGCCCGGCGTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTGCACATACAGGCGGCAGGCTGCGGTGCAGTCCTGGCCGGCGTTGTAGaa                                                  <  8:88186/149‑1 (MQ=255)
                                                                                                        cccAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTGCACATACAGGCGGCAGGCTGCGGTGTAGTCCTGGCCGGCGTTGTAGAAGc                                                >  3:206637/1‑145 (MQ=255)
                                                                                                        cccAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTGCACATACAGGCGGCAGGCTGCGGTGTAGTCCTGGCCGGCGTTGTAGAAGc                                                <  4:206637/145‑1 (MQ=255)
                                                                                                         ccAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTc                                                                                                     <  5:85837/91‑1 (MQ=255)
                                                                                                         ccAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTc                                                                                                     >  6:85837/1‑91 (MQ=255)
                                                                                                               cGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTGCACATACAGGCGGCAGGCTGCGGTGCAGTCCTGGCCGGCGTTGTAGAAGCCGAAGGTGCg                                      >  7:284492/1‑148 (MQ=255)
                                                                                                                      ttcAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTGCACATACAGGCGGCAGGCTGCGGTGCAGTCCTGGCCGGCGTTGTAGAAGCCGAAGGTGc                                       >  5:282893/1‑140 (MQ=255)
                                                                                                                      ttcAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTGCACATACAGGCGGCAGGCTGCGGTGCAGTCCTGGCCGGCGTTGTAGAAGCCGAAGGTGc                                       <  6:282893/140‑1 (MQ=255)
                                                                                                                      ttcAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTGCACATACAGGCGGCAGGCTGCGGTGCAGTCCTGGCCGGCGTTGTAGAAGCCGAAGGTGCGGATGCCGt                              >  5:592/1‑149 (MQ=255)
                                                                                                                           aCTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTGCACATACAGGCGGCAGGCTGCGGTGCAGTCCTGGCCGGCGTTGTAGAAGCCGAAGGTGCGGATGCCGTCg                            >  1:5425/1‑146 (MQ=255)
                                                                                                                           aCTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTGCACATACAGGCGGCAGGCTGCGGTGCAGTCCTGGCCGGCGTTGTAGAAGCCGAAGGTGCGGATGCCGTCg                            <  2:5425/146‑1 (MQ=255)
                                                                                                                           aCTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTGCACATACAGGCGGCAGGCTGCGGTGCAGTCCTGGCCGGCGTTGTAGAAGCCGAAGGTGCGGATGCCGTCGATg                         >  4:199714/1‑149 (MQ=255)
                                                                                                                                      cccGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTGCACATACAGGCGGCAGGCTGCGGTGCAGTCCTGGCCGGCGTTGTAGAAGCCGAAGGTGCGGATGCCGTCGATGGCGGCATCGAt              <  5:289726/149‑1 (MQ=255)
                                                                                                                                       ccGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTGCACATACAGGCGGCAGGCTGCGGTGCAGTCCTGGCCTGCGTTGTAGAAGCCGAAGGTGCGGATGCCGTCGATGGCGGCATCGATg             >  8:168647/1‑149 (MQ=255)
                                                                                                                                       ccGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTGCACATACAGGCGGCAGGCTGCGGTGCAGTCCTGGCCGGCGTTGTAGAAGc                                                <  6:166771/114‑1 (MQ=255)
                                                                                                                                       ccGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTGCACATACAGGCGGCAGGCTGCGGTGCAGTCCTGGCCGGCGTTGTAGAAGc                                                >  5:166771/1‑114 (MQ=255)
                                                                                                                                            ttGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTGCACATACAGGCGGCAGGCTGCGGTGCAGTCCTGGCCGGCGTTGTAGAAGCCGAAGGTGCGGATGCCGTCGATGGCGGCATCGATGTCGGc        >  4:268688/1‑149 (MQ=255)
                                                                                                                                             tGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTGCACATACAGGCGGCAGGCTGCGGTGCAGTCCTGGCCGGCGTTGTAGAAGCCGAAGGTGCGGATGCCGTCGATGGCGGCATCGATGTCAGc        >  7:113925/1‑148 (MQ=255)
                                                                                                                                             tGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTGCACATACAGGCGGCAGGCTGCGGTGCAGTCCTGGCCGGCGTTGTAGAAGCCGAAGGTGCGGATGCCGTCGATGGCGGCATCGATGTCAGc        <  8:113925/148‑1 (MQ=255)
                                                                                                                                                ggctggctACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTGCACATACAGGCGGCAGGCTGCGGTGCAGTCCTGGCCGGCGTTGTAGAAGCCGAAGGTGCGGATGCCGTCGATGGCGGCATCGATGTCAGCGTCa    >  1:72802/1‑149 (MQ=255)
                                                                                                                                                 gctggctACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTGCACATACAGGCGGCAGGCTGCGGTGCAGTCCTGGCCGGCGTTGTAGAAGCCGAAGGTGCGGATGCCGTCGATGGCGGCATCGATGTCAGCGTCAt   >  1:254277/1‑149 (MQ=255)
                                                                                                                                                 gctggctACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTGCACATACAGGCGGCAGGCTGCGGTGCAGTCCTGGCCGGCGTTGTAGAAGCCGAAGGTGCGGATGCCGTCGATGGCGGCATCGATGTCAGCGTCAt   >  3:65806/1‑149 (MQ=255)
                                                                                                                                                  ctggctACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTGCACATACAGGCGGCAGGCTGCGGTGCAGTCCTGGCCGGCGTTGTAGAAGCCGAAGGTGCGGATGCCGTCGATGGCGGCATCGATGTCAGCGTCATc  <  6:109214/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CCGCTATTGGCTTGCCACCGGTTACCAGACGAATGTGCGGCTGGGCGATGGCCCGCTGCACCAGCCCGGCGACCTTGTCGCGGTGCTGGGCGCTGATCAGTGGCCCCAGTTCGGTTTCTTCAGACTCCTGCAGGCCCGGCTTGAGGCTGGCTACGGCTTTACCCAGGCGTTCGACAAAGCGCTCGTAGATCCCGTCCTGCACATACAGGCGGCAGGCTGCGGTGCAGTCCTGGCCGGCGTTGTAGAAGCCGAAGGTGCGGATGCCGTCGATGGCGGCATCGATGTCAGCGTCATC  >  NC_002947/1685716‑1686010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑