Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,703,284 A→G 10.3% intergenic (+133/‑34) lysS → / → PP_1497 lysine‑‑tRNA ligase/TetR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,703,2840AG10.3% 100.7 / 3.1 40intergenic (+133/‑34)lysS/PP_1497lysine‑‑tRNA ligase/TetR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (19/16);  new base G (2/2);  total (22/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.50e-01

GAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCAAGGCCGCAAGACTGCCCGCCAAGGCAGCGAACAGCGTCGCC  >  NC_002947/1703159‑1703429
                                                                                                                              |                                                                                                                                                 
gAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCAt                                                                                                                              >  2:55479/1‑147 (MQ=255)
     aGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTTAAACCGCGCCTAAAAAGGCC‑GTGTTGGAGCGGGGTTTCCCGCGCAGGGGGCCGGACAGACGAAACCCCCCCCTGAGGtt                                                                                                                        >  5:23948/1‑147 (MQ=255)
      gccgccTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTTCAAAGGGC‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATc                                                                                                                      >  7:97343/1‑149 (MQ=255)
            tttCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACGGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTAACCGCGAAGGGGCCAGCACTGACGAAACACCCCCAAGAGGTATACCCCGt                                                                                                                >  1:247252/1‑149 (MQ=255)
            tttCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCa                                                                                                                                                >  7:109781/1‑117 (MQ=255)
                        tttttCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCAt                                                                                                    >  2:85144/1‑149 (MQ=255)
                         ttttCTTGCATTATGTTTTTTTTTTTCTTTACCGCCCGGTGCGGGGGTAAACCGGCTCATACAATGGCT‑TTGTTGTTGGGGTTTAACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCAt                                                                                                    <  2:247252/148‑1 (MQ=255)
                                cccTGAAGATTTATGTGTTCATTCCCGCCCTCTGCGGGGGTAACCCCGCACCTACAACGGCC‑TTGTCGGTGCGGTTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGAc                                                                                             <  8:97343/147‑1 (MQ=255)
                                         tttATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGGCAGCACTGACGAAACACCCCCATGAGGGATCGCCCGTGACACCCGCCCTGCCTGCCCAAGGcgccg                                                                                   >  3:115736/1‑149 (MQ=37)
                                           tATGTTGTCAGTACCGGCCTCTTCGCGGGTTAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAAAACCCCCAAGCGGTATCCCCCGTGACACCCGCCCTGCCGGGCCCAAGCgccgcc                                                                                 >  4:297799/1‑149 (MQ=25)
                                             tgttgtCAGACCCGGCCTCTCCGCGGGAAACCCCGCCCCAACAACGCCC‑TTGTCGGAGCGGTTTAACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGccgccg                                                                                <  3:297799/148‑1 (MQ=255)
                                                                  tccgGGAAAACCCCCCCCCACCAAGGCCGTTGTCGTGGGGGGTTACCCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGcc                                                            <  6:23948/146‑1 (MQ=255)
                                                                  cgcgGGTAAACCCGCTCCTACAAGGCCT‑TTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCAt                                                                            <  8:67317/131‑1 (MQ=25)
                                                                  cgcgGGTAAACCCGCTCCTACAAGGCCT‑TTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCAt                                                                            >  7:67317/1‑131 (MQ=25)
                                                                             ccGCTCCTACAAGGCCT‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCa                                                                                           >  3:317121/1‑105 (MQ=255)
                                                                             ccGCTCCTACAAGGCCT‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCa                                                                                           <  4:317121/105‑1 (MQ=255)
                                                                             ccGCTCCTACAAGGCCT‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCaa                                                >  7:74236/1‑148 (MQ=255)
                                                                             ccGCTCCTACAAGGCCT‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCaa                                                >  3:61518/1‑148 (MQ=255)
                                                                             ccGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCaa                                                >  2:293834/1‑148 (MQ=255)
                                                                              cGCTCCTACAAGGCCT‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATa                                              <  8:74236/149‑1 (MQ=255)
                                                                               gCTCCTACAAGGCCT‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATAc                                             >  6:189657/1‑149 (MQ=255)
                                                                                cTCCTACAAGGCCT‑TTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGACAAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCaa                                                                                          <  4:255698/103‑1 (MQ=18)
                                                                                cTCCTACAAGGCCT‑TTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGACAAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCaa                                                                                          >  3:255698/1‑103 (MQ=18)
                                                                                   cTACAAAGGCC‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTg                                                   <  3:214607/139‑1 (MQ=255)
                                                                                   cTACAAAGGCC‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTg                                                   >  4:214607/1‑139 (MQ=255)
                                                                                   cTACAAAGGCC‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCAAg                                         <  1:85144/149‑1 (MQ=255)
                                                                                                  tAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCAAGGCCGCAAGACTGc                            <  1:55479/148‑1 (MQ=255)
                                                                                                       gCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCaa                                          <  1:243913/129‑1 (MQ=255)
                                                                                                       gCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCaa                                          >  2:243913/1‑129 (MQ=255)
                                                                                                       gCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCAAGGCCGCAAGACTGCCCGcc                       >  3:299860/1‑148 (MQ=255)
                                                                                                        cGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCAAGGCCGCAAGACTGCCCGCCaa                     <  4:61518/149‑1 (MQ=255)
                                                                                                          ggTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCAAGGCCGCAAGACTGCCCGCCAAgg                   >  2:28115/1‑149 (MQ=255)
                                                                                                                cccGCGATGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCAAGGCCGCAAGACTGCCCGCCAAGGCAGCg              <  1:293834/148‑1 (MQ=255)
                                                                                                                 ccGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCAAGGCCGCAAGACTGCCCGCCAAGGCAGCGaa            <  4:115736/149‑1 (MQ=255)
                                                                                                                   gcgAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCAAGGCCGCAAGACTGCCCGCCAAGGCAGCGaa            >  4:24490/1‑147 (MQ=255)
                                                                                                                   gcgAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCAAGGCCGCAAGACTGCCCGCCAAGGCAGCGAACa          <  5:189657/149‑1 (MQ=255)
                                                                                                                        ggggCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCAAGGCCGCAAGACTGCCCGCCAAGGCAGCGAACAGCGTc     <  1:28115/149‑1 (MQ=255)
                                                                                                                        ggggCCAGCACTGACGAAACACACCCATGAGGTATACCCCGTGACACCCGCCATGCCTGACCAACGCGCCGCCGCCATCGCCACCGCCGTCGCCGAAAGCGAGCAAAACCAAGGCCGCAAGACTCCCCGCCAAGCCAGCGAACAGCgac     >  4:339388/1‑147 (MQ=255)
                                                                                                                           gCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACGGCCGTCGCCGAAAGCGTGCAATACCAAGGCCGCAAGACTGCCCGCCAAGGCAGCGAACAGCGTCGcc  <  3:339388/149‑1 (MQ=255)
                                                                                                                           gCCAGCACTGACGAAACACACCCATGAGGTATCCCCCGTGACACCAGCCATGCCTGACCAAGGCGCCGCCGGAATCTCCACCGCCGTCGCCGAAAGCGTGCAATACCAAGGCCGCAAGACTGCCCGCCAAGGCAGCGAACAGCGTCGc   >  2:61654/1‑148 (MQ=255)
                                                                                                                              |                                                                                                                                                 
GAACGAGCCGCCTTTCGAGGCGGCTTTTTCTTGCCTGAAGCTTTATGTTGTCAGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGGCC‑TTGTAGGAGCGGGTTTACCCGCGAAGGGGCCAGCACTGACGAAACACCCCCATGAGGTATCCCCCGTGACACCCGCCATGCCTGACCAAGGCGCCGCCGGCATCGCCACCGCCGTCGCCGAAAGCGTGCAATACCAAGGCCGCAAGACTGCCCGCCAAGGCAGCGAACAGCGTCGCC  >  NC_002947/1703159‑1703429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑