| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,719,292 | T→C | 7.2% | intergenic (+53/+77) | PP_1514 → / ← PP_1515 | hypothetical protein/Transcriptional regulator, tetR family |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,719,292 | 0 | T | C | 7.2% | 154.1 / 2.2 | 55 | intergenic (+53/+77) | PP_1514/PP_1515 | hypothetical protein/Transcriptional regulator, tetR family |
| Reads supporting (aligned to +/- strand): ref base T (24/27); new base C (2/2); total (26/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01 | |||||||||||
CGGTTAATGCTCGTATGTCCGCAGCGGGATCCTCGCGAGACCTAATGATTCAAGCCGCGTTAATAGCAGAGATCAGAAGGCGTCCCGCATAAATGGCTTGGGTGTGATGAACTCTATATCAGCTACCTTCTGCGTGCACTCACGTAGAAGGCAGAAGGCAGAA‑GGCAGAAGGCAGAAGTTTGGCTAGTGAGCGATTGAAAAAGTATTAATGAAGATTTTTCTCAGTCCCTAAACGCCCGTAAGAACACTTTTACCACCTCGCGCACATGTGCCTCGGCTTCATCGC > NC_002947/1719148‑1719433 | cGGTTAATGCTCGTATGTCCGCAGCGGGATCCTCGCGAGACCTAATGATTCAAGCCGCGTTAATAGCAGAGATCAGAAGGCGTCCCGCATAAATGGCTTGGGTGTGATGAACTCTATATCAGCTACCTTCTGCGTGCACTCACGTagaa < 1:302847/149‑1 (MQ=255) gTTAATGCTCGTATGTCCGCAGCGGGATCCTCGCGAGACCAAATGATTCAAGCCGCGTTAATAGCAGAGATCAGAAGGCGTCCCGCATAAATGGCTTTGGTGTGATGAACTCTATATCAGCTACCTTCTACGTGAGTGCACGCagaagg < 6:180019/149‑1 (MQ=255) aaTGCTCGTATGTCCGCAGCGGGATCCTCGCGAGACCTAATGATTCAAGCCGCGTTAATAGCAGAGATCAGAAGGCGTCCCGCATAAATGGCTTGGGTGTGATGAACTCTATATCAGCTACCTTCTGCGTGCACTCACGTa < 1:41262/141‑1 (MQ=255) aaTGCTCGTATGTCCGCAGCGGGATCCTCGCGAGACCTAATGATTCAAGCCGCGTTAATAGCAGAGATCAGAAGGCGTCCCGCATAAATGGCTTGGGTGTGATGAACTCTATATCAGCTACCTTCTGCGTGCACTCACGTa > 2:41262/1‑141 (MQ=255) tGCTCGTATGTCCGCAGCGGGATCCTCGCGAGACCTAATGATTCAAGCCGCGTTAATAGCAGAGATCAGAAGGCGTCCCGCATAAATGGCTTGGGTGTGATGAACTCTATATCAGCTACCTTCTGCGTGCACTCACGTagaaggcag > 3:347541/1‑147 (MQ=255) gCTCGTATGTCCGCAGCGGGATCCTCGCGAGACCTAATGATTCAAGCCGCGTTAATAGCAGAGATCAGAAGGCGTCCCGCATAAATGGCTTGGGTGTGATGAACTCTATATCAGCTACCTTCTACGTGAGTGCACGCagaaggtagct > 6:175794/1‑146 (MQ=255) tATGTCCGCAGCGGGATCCTCGCGAGACCTAATGATTCAAGCCGCGTTAATAGCAGAGATCAGAAGGCGTCCCGCATAAATGGCTTGGGTGTGATGAACTCTATATCAGCTACCTTCTACGTGAGTGCACGCagaaggt < 5:140275/139‑2 (MQ=255) tATGTCCGCAGCGGGATCCTCGCGAGACCTAATGATTCAAGCCGCGTTAATAGCAGAGATCAGAAGGCGTCCCGCATAAATGGCTTGGGTGTGATGAACTCTATATCAGCTACCTTCTACGTGAGTGCACGCagaaggt > 6:140275/1‑138 (MQ=255) aTGTCCGCAGCGGGATCCTCGCGAGACCTAATGATTCAAGCCGCGTTAATAGCAGAGATCAGAAGGCGTCCCGCATAAATGGCTTGGGTGTGATGAACTCTATATCAGCTACCTTCTGCGTGCACTCACGTagaaggcagaatgaag > 8:239722/1‑142 (MQ=255) tCCGCAGCGGGATCCGCTCGAGACATAATGATTAAAGCCGAGTTAATAGCAGAGATCAGAAGGCGTCCCGCATAAATGGCTTGGGTGTGATTAACTCTATATCAGCTACCTTCTGCGTGCACTCACGTagaaggcagaaggcagaa‑ggc < 8:204942/149‑1 (MQ=255) cGCAGCGGGATCCTCGCGAGACCTAATGATTCAAGCCGCGTTAATAGCAGAGATCAGAAGGCGTCCCGCATAAATGGCTTGGGTGTGATGAACTCTATATCAGCTACCTTCTGCGTGCACTCACGTagaaggcagaaggcagaa‑gg > 6:127721/1‑146 (MQ=255) cAGCGGGATCCTCGCGAGACCTAATGATTCAAGCCGCGTTAATAGCAGAGATCAGAAGGCGTCCCGCATAAATGGCTTGGGTGTGATGAACTCTATATCAGCTACCTTCTGCGTGCACTCACGTagaa > 2:26721/1‑128 (MQ=255) cAGCGGGATCCTCGCGAGACCTAATGATTCAAGCCGCGTTAATAGCAGAGATCAGAAGGCGTCCCGCATAAATGGCTTGGGTGTGATGAACTCTATATCAGCTACCTTCTGCGTGCACTCACGTagaa < 1:26721/128‑1 (MQ=255) cGGGATCCTCGCGAGACCTAATGATTCAAGCCGCGTTAATAGCAGAGATCAGAAGGCGTCCCGCATAAATGGCTTGGGTGTGATGAACTCTATATCAGCTACCTTCTGCGTGCACTCACGTagaaggcagaaggcagaa‑ggcagaaggc < 4:347541/149‑1 (MQ=255) ggATCCTCGCGAGACCTAATCATTCAAGCCGCGTTAATAGCAGAGATCAGAAGGCGTCCCGCATAAATGGCTTGGGTGTGATGAACTCTATATCAGCTACCTTCTGCGTGCACTCACGTAGaaggcagaaggcagaa‑ggcagaaggcag < 3:290244/149‑1 (MQ=255) cTAATGATTCAAGCCGCGTTAATAGCAGAGATCAGAAGGCGTCCCGCATAAATGGCTTGGGTGTGATGAACTCTATATCAGCTACCTTCTGCGTGCACTCACGTAGAAggcagaaggcagaa‑ggcagaaggcagaa > 7:164056/1‑136 (MQ=255) cTAATGATTCAAGCCGCGTTAATAGCAGAGATCAGAAGGCGTCCCGCATAAATGGCTTGGGTGTGATGAACTCTATATCAGCTACCTTCTGCGTGCACTCACGTAGAAggcagaaggcagaa‑ggcagaaggcagaa < 8:164056/136‑1 (MQ=255) aTGATTCAAGCCGCGTTAATAGCAGAGATCAGAAGGCGTCCCGCATAAATGGCTTGGGTGTGATGAACTCTATATCAGCTACCTTCTGCGTGCACTCACGTAGAAGGCAGAAGGCAGAA‑GGCAGAAGGCAGAAGTTTGGCTAGTGAGc < 6:207159/148‑1 (MQ=255) tGATTCAAGCCGCGTTAATAGCAGAGATCAGAAGGCGTCCCGCATAAATGGCTTGGGTGTGATGAACTCTATATCAGCTACCTTCTGCGTGCACTCACGTAGAAGGCAGAAGGCAGAA‑GGCAGAAGGCAGAAGTTTGGCTAGTGAGCGa < 5:35305/149‑1 (MQ=255) aTTCAAGCCGCGTTAATAGCAGAGATCAGAAGGCGTCCCGCATAAATGGCTTGGGTGTGATGAACTCTATATCAGCTACCTTCTGCGTGCACTCACGTAGAAGGCAGAAGGCAGAA‑GGCAGAAGGCAGAAGTTTGGCTAGTGAGCGAtt < 8:236532/149‑1 (MQ=255) aTTCAAGACGCGTTAATAGCAGAGATCAGAAGGCGTCCCGCATAAATGGCTTGGGTGTGATGAACTCTATATCAGCTACCTTCTGCGTGCACTCACGTAGAAGGCAGAAGGCAGAA‑GGCAGAAGGCAGAAGTTTGGCTAGTGAGCGAt < 6:268256/148‑1 (MQ=255) aaGCCGCGTTAATAGCAGAGATCAGAAGGCGTCCCGCATAAATGGCTTGGGTGTGATGAACTCTATATCAGCTACCTTCTGCGTGCACTCACGTAGAAGGCAGAAGGCAGAA‑GGCAGAAGGCAGAAGTTTGGCTAGTGAGCGATTGaa > 5:117443/1‑148 (MQ=255) gTTAATAGCAGAGATCAGAAGGCGTCCCGCATAAATGGCTTGGGTGTGATGAACTCTATATCAGCTACCTTCTGCGTGCACTCACGTAGAAGGCAGAAGGCAGAA‑GGCAGAAGGCAGAAGTTTGGCTAGTGAGCGATTGAAAAAGTAtt < 5:127721/149‑1 (MQ=255) aTAGCAGAGATCAGAAGGCGTCCCGCATAAATGGCTTGGGTGTGATGAACTCTATATCAGCTACCTTCTGCGTGCACTCACGTAGAAGGCAGAAGGCAGAA‑GGCAGAAGGCAGAAGTTTGGCTAGTGAGCGATTGAAAAAGTATTaa > 5:34546/1‑147 (MQ=255) aTAGCAGAGATCAGAAGGCGTCCCGCATAAATGGCTTGGGTGTGATGAACTCTATATCAGCTACCTTCTGCGTGCACTCACGTAGAAGGCAGAAGGCAGAA‑GGCAGAAGGCAGAAGTTTGGCTAGTGAGCGATTGAAAAAGTATTaa < 6:34546/147‑1 (MQ=255) agaTCAGAAGGCGTCCCGCATAAATGGCTTGGGTGTGATGAACTCTATATCAGCTACCTTCTGCGTGCACTCACGTagaaggcagaaggcagaa‑ggcagaaggc < 1:164378/104‑1 (MQ=255) agaTCAGAAGGCGTCCCGCATAAATGGCTTGGGTGTGATGAACTCTATATCAGCTACCTTCTGCGTGCACTCACGTagaaggcagaaggcagaa‑ggcagaaggc > 2:164378/1‑104 (MQ=255) aGAAGGCGTCCCGCATAAATGGCTTGGGTGTGATGAACTCTATATCAGCTACCTTCTGCGTGCACTCACGTagaaggcagaaggcagaa‑g < 7:9298/90‑1 (MQ=255) aGAAGGCGTCCCGCATAAATGGCTTGGGTGTGATGAACTCTATATCAGCTACCTTCTGCGTGCACTCACGTagaaggcagaaggcagaa‑g > 8:9298/1‑90 (MQ=255) aaGGCGTCCCGCATAAATGGCTTGGGTGTGATGAACTCTATATCAGCTACCTTCTGCGTGCACTCACGTagaaggc > 4:148520/1‑76 (MQ=255) aaGGCGTCCCGCATAAATGGCTTGGGTGTGATGAACTCTATATCAGCTACCTTCTGCGTGCACTCACGTagaaggc < 3:148520/76‑1 (MQ=255) aaGGCGTCCCGCATAAATGGCTTGGGTGTGATGAACTCTATATCAGCTACCTTCTGCGTGCACTCACGTAGAAGGCAGAAGGCAGAA‑GGCAGAAGGCAGAAGTTTGGCTAGTGAGCGa > 4:83164/1‑118 (MQ=255) ccGCATAAATGGCTTGGGTGTGATGAACTCTATATCAGCTACCTTCTGCGTGCACTCACGTAGAAGGCAGAAGGCAGAA‑GGCAGAAGGCAGAAGTTTGGCTAGTGAGCGATTGAAAAAGTATTaa < 1:287323/125‑1 (MQ=255) ccGCATAAATGGCTTGGGTGTGATGAACTCTATATCAGCTACCTTCTGCGTGCACTCACGTAGAAGGCAGAAGGCAGAA‑GGCAGAAGGCAGAAGTTTGGCTAGTGAGCGATTGAAAAAGTATTaa > 2:287323/1‑125 (MQ=255) tAAAAGGCTTGGGTGTGATGAACTCTATATCAGCTACCTTCTGCGTGCACTCACGTAGAAGGCAGAAGGCAGAA‑GGCAGAAGGCAGAAGTTTGGCTAGTGAGCGATTGAAAAAGTATTAATGAAGATTTTTCTCAGTCCCTAAACGccc < 8:141671/149‑1 (MQ=255) aaTGGCTTGGGTGTGATGAACTCTATATCAGCTACCTTCTGCGTGCACTCACGTAGAAGGCAGAAGGCAGAA‑GGCAGAAGGCAGAAGTTTGGCTAGTGAGCGATTGAAAAAGTATTAATGAAGATTTTtc < 5:223300/130‑1 (MQ=255) aaTGGCTTGGGTGTGATGAACTCTATATCAGCTACCTTCTGCGTGCACTCACGTAGAAGGCAGAAGGCAGAA‑GGCAGAAGGCAGAAGTTTGGCTAGTGAGCGATTGAAAAAGTATTAATGAAGATTTTtc > 6:223300/1‑130 (MQ=255) aTGGCTTGGGTGTGATGAACTCTATTTCAGCTACCTTCTGCGTGCACTCACGTAGAAGGCAGAAGGCAGAA‑GGCAGAAGGCAGAAGTTTGGCTAGTGAGCGATTGAAAAAGTATTAATGAAGATTTTTCTCAGTCCCTAAACGCCCGt > 2:226190/1‑148 (MQ=255) tGGCTTGTGTGTGATGAACTCTATATCAGCTACCTTCTGCGTGCACTCAAGTAGAAGGCAGAAGGCAGAA‑GGCAGAAGGCAGAAGTTTGGCTAGTGAGCGATTGAAAAAGTATTAATGAAGATTTTTCTCAGTCCCTAAACGCCCGTaa < 7:239722/149‑1 (MQ=255) tgattaAATCTATATCATCTACCTTCTGCTTTCACTCAAGTATAATGCAGAAGGCAGAA‑GGCAGAAGGCATAAGTTTGGCTAGTGAGCGATTGAAAAAGTATTAATGAAGATTTTTCTCAGTCCCTAAACGCCCGTAAGAACACtttt < 5:349971/148‑1 (MQ=255) tgatgaACTCTATATCAGCTACCTTCTGCGTGCACTCACGTAGAAGGCAGAAGGCAGAA‑GGCAGAAGGCAGAAGTTTGGCTAGTGAGCGATTGAAAAAGTATTAATGAAGATTTTTCTCAGTCCCTAAACGCCCGTAAGAACACTTTTa > 2:290363/1‑149 (MQ=255) ctctATATCAGCTACCTTCTGCGTGCACTCACGTAGAAGGCAGAAGGCAGAA‑GGCAGAAGGCAGAAGTTTGGCTAGTGAGCGATTGAAAAAGTATTAATGAAGATTTTTCTCAGTCCCTAAACGCCCGTAAGAACACTTTTACAACCTc > 4:119043/1‑149 (MQ=255) tattTCAGCTACCTTCTGCGTGCACTCACGTAGAAGGCAGAAGGCAGAA‑GGCAGAAGGCAGAAGTTTGGCTAGTGAGCGATTGAAAAAGTATTAATGAAGATTTTTCTCAGTCCCTAAACGCCCGTAAGAACACTTTTACCACCTcg < 1:226190/147‑1 (MQ=255) aGCTACCTTCTGCGTGCACTCACGTAGAAGGCAGAAGGCAGAA‑GGCAGAAGGCAGAAGTTTGGCTAGTGAGCGATTGAAAAAGTATTAATGAAGATTTTTCTCAGTCCCTaaa > 6:214217/1‑113 (MQ=255) aGCTACCTTCTGCGTGCACTCACGTAGAAGGCAGAAGGCAGAA‑GGCAGAAGGCAGAAGTTTGGCTAGTGAGCGATTGAAAAAGTATTAATGAAGATTTTTCTCAGTCCCTaaa < 5:214217/113‑1 (MQ=255) cTTCTGCGTGCACTCACGTAGAAGGCAGAAGGCAGAA‑GGCAGAAGGCAGAAGTTTGGCTAGTGAGCGATTGAAAAAGTATTAATGAAGATTTTTCTCAGTCCCTAAACGCCCGTAAGAACACTTTTACCACCTCGCGCACATGTGcc > 2:307897/1‑147 (MQ=255) cTTCTGCGTGCACTCACGTAGAAGGCAGAAGGCAGAA‑GGCAGAAGGCAGAAGTTTGGCTAGTGAGCGATTGAAAAAGTATTAATGAAGATTTTTCTCAGTCCCTAAACGCCCGTAAGAACACTTTTACCACCTCGCGCACATGTGcc < 1:307897/147‑1 (MQ=255) gCGTGCACTCACGTAGAAGGCAGAAGGCAGAA‑GGCAGAAGGCAGAAGTTTGGCTAGTGAGCGATTGAAAAAGTATTAATGAAGATTTTTCTCAGTCCCTAAACGCCCGTAAGAACACTTTTACCACCTCGCGCACATGTGCCTCGGCt > 7:1750/1‑148 (MQ=255) cGTGCACTCACGTAGAAGGCAGAAGGCAGAAGGGCAGAAGGCAGAAGTTTGGCTAGTGAGCGATTGAAAAAGTATTAATGAAGATTTTTCTCAGTcc > 1:42582/1‑97 (MQ=255) cGTGCACTCACGTAGAAGGCAGAAGGCAGAA‑GTGAGAAGGCAGAAGTTTGGCTAGTGAGCGATTGAAAAAGTATTAATGAAGATTTTTCTCAGTcc < 2:42582/96‑1 (MQ=255) cGTGCACTCACGTAGAAGGCAGAAGGCAGAA‑GGCAGAAGGCAGAAGTTTGGCTAGTGAGCGATTGAAAAAGTATTAATGAAGATTTTTCTCAGTCCCTAAACGCCCGTAAGAACACTTTTACCACCTCGCGCACATGTGCCTCGGCtt > 2:175344/1‑148 (MQ=255) gTGCACTCACGTAGAAGGCAGAAGGCAGAA‑GGCAGAAGGCAGAAGTTTGGCTAGTGAGCGATTGAAAAAGTATTAATGAAGATTTTTCTCAGTCCCTAAACGCCCGt > 2:272715/1‑107 (MQ=255) aCTCACGTAGAAGGCAGAAGGCAGAA‑GGCAGAAGGCAGAAGTTTGGCTAGTGAGCGATTGAAAAAGTATTAATGAAGATTTTTCTCAGTCCCTAAACGCCCGTAAGAACACTTTTACCACCTCGCGCACATGTGCCTCGGCTTCATCGc < 3:119043/149‑1 (MQ=255) gTAGAAGGCAGAAGGCAGAA‑GGCAGAAGGCAGAAGTTTGGCTAGTGAGCGATTGAAAAAGTATTAATGAAGATTTTTCTCAGTCCCTAAACGCCCGTAAGAACACtt > 3:249463/1‑107 (MQ=255) gTAGAAGGCAGAAGGCAGAA‑GGCAGAAGGCAGAAGTTTGGCTAGTGAGCGATTGAAAAAGTATTAATGAAGATTTTTCTCAGTCCCTAAACGCCCGTAAGAACACtt < 4:249463/107‑1 (MQ=255) | CGGTTAATGCTCGTATGTCCGCAGCGGGATCCTCGCGAGACCTAATGATTCAAGCCGCGTTAATAGCAGAGATCAGAAGGCGTCCCGCATAAATGGCTTGGGTGTGATGAACTCTATATCAGCTACCTTCTGCGTGCACTCACGTAGAAGGCAGAAGGCAGAA‑GGCAGAAGGCAGAAGTTTGGCTAGTGAGCGATTGAAAAAGTATTAATGAAGATTTTTCTCAGTCCCTAAACGCCCGTAAGAACACTTTTACCACCTCGCGCACATGTGCCTCGGCTTCATCGC > NC_002947/1719148‑1719433 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |