Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,748,903 A→G 8.3% intergenic (‑236/‑96) PP_1548 ← / → PP_5744 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,748,9030AG 8.3% 163.3 / 4.6 60intergenic (‑236/‑96)PP_1548/PP_5744hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (25/30);  new base G (2/3);  total (27/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGTCGAGCGAGGAAGCTTGCTACGTTGCGTAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGCCTT  >  NC_002947/1748761‑1749046
                                                                                                                                              |                                                                                                                                                
aTGTCGAGCGAGGAAGCTTGCTACGTTGCGTAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCa                                                                                                                                            >  4:212436/1‑149 (MQ=255)
   tCGAGCGAGGAAGCTTGCTACGTTGCGTAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCAtt                                                                                                                                          >  8:206803/1‑148 (MQ=255)
     gagcgagGAAGCTTGCTACGTTGCGTAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGTTCGCTTGCAtttgc                                                                                                                                       <  8:114875/149‑3 (MQ=255)
     gagcgagGAAGCTTGCTACGTTGCGTAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTtat                                                                                                                                       <  4:108592/149‑1 (MQ=255)
     gagcgagGAAGCTTGCTACGTTGCGTAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTta                                                                                                                                        <  3:6629/148‑1 (MQ=255)
         gagGAAGCTTGCTACGTTGCGTAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATaaaa                                                                                                                                   <  8:157092/149‑1 (MQ=255)
         gagGAAGCTTGCTACGTTGCGTAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATaaaa                                                                                                                                   <  7:69963/149‑1 (MQ=255)
               gCTTGCTACGTTGCGTAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGc                                                                                                                             <  6:328648/149‑1 (MQ=255)
                cTTGCTACGTTGCGTAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGTGCTTGCATTTaaaaaacaagcat                                                                                                                            >  3:244498/1‑142 (MQ=37)
                 ttGCTACGTTGCGTAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCtt                                                                                                                           >  3:179831/1‑149 (MQ=255)
                     tACGTTGCGTAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑ttt                                                                                                                      >  6:120577/1‑149 (MQ=255)
                       cGTTGCGTAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑ttttt                                                                                                                    <  8:265388/149‑1 (MQ=255)
                              tAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGc                                                                                                             <  4:179831/149‑1 (MQ=255)
                               aaCGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGc                                                                                                             >  8:159401/1‑148 (MQ=255)
                                aCGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCaa                                                                                                           <  5:120577/149‑1 (MQ=255)
                                  gTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑ttt                                                                                                                      >  6:58199/1‑136 (MQ=255)
                                  gTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑ttt                                                                                                                      <  5:58199/136‑1 (MQ=255)
                                     caccaGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCAc                                                                                                       <  7:206803/148‑1 (MQ=255)
                                      accaGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCACGc                                                                                                     >  5:110193/1‑149 (MQ=255)
                                       ccaGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCACGCt                                                                                                    >  6:202511/1‑149 (MQ=255)
                                       ccaGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCACGCt                                                                                                    >  6:197513/1‑149 (MQ=255)
                                        caGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCACGCtt                                                                                                   <  8:37723/149‑1 (MQ=255)
                                         aGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCACGCTtg                                                                                                  >  8:235705/1‑149 (MQ=255)
                                          gAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCACGCTtgt                                                                                                 <  3:294150/149‑1 (MQ=255)
                                          gAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCACGCTtgt                                                                                                 <  5:197513/149‑1 (MQ=255)
                                          gAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCACGCTtgt                                                                                                 <  5:202511/149‑1 (MQ=255)
                                          gAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCACGCTtgt                                                                                                 <  6:110193/149‑1 (MQ=255)
                                             cTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGTGCTTGCATTTA‑AAAAACAAGCATGCTTTTATAAATGCAAGTTCGCTtgcatt                                                                                              <  4:244498/149‑5 (MQ=21)
                                               caggcGCCGAGTTAATCCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAAt                                                                                            >  2:17693/5‑149 (MQ=39)
                                                   cGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTtc                                                                                        >  2:171509/1‑149 (MQ=255)
                                                             aCTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGt                                                                               <  3:212436/148‑1 (MQ=255)
                                                               tCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATg                                                                            >  5:330880/1‑149 (MQ=255)
                                                                  gcgcCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGAt                                                                         <  7:235705/149‑1 (MQ=255)
                                                                    gcCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCa                                                                                                                                >  1:142431/1‑93 (MQ=255)
                                                                    gcCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCa                                                                                                                                <  2:142431/93‑1 (MQ=255)
                                                                    gcCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATgg                                                                           <  8:119962/145‑1 (MQ=255)
                                                                    gcCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATgg                                                                           >  7:119962/1‑145 (MQ=255)
                                                                                aTCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAgg                                                           <  3:308942/149‑1 (MQ=255)
                                                                                    gctgGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCt                                                       >  8:9501/1‑149 (MQ=255)
                                                                                     ctgGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATgg                                                                           >  1:248125/1‑128 (MQ=255)
                                                                                     ctgGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATgg                                                                           <  2:248125/128‑1 (MQ=255)
                                                                                        gTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCttt                                                     <  7:159401/147‑1 (MQ=255)
                                                                                        gTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTc                                                    <  6:330880/148‑1 (MQ=255)
                                                                                         tttttAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCgg                                                  <  1:171509/149‑1 (MQ=255)
                                                                                            ttAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGAt                                                <  1:17693/148‑1 (MQ=255)
                                                                                              aaaGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTc                                             >  4:18821/1‑149 (MQ=255)
                                                                                                      gTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAgg                                     <  3:18821/149‑1 (MQ=255)
                                                                                                           tGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGaaa                                  >  2:242259/1‑147 (MQ=255)
                                                                                                                gAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGt                           >  6:284524/1‑149 (MQ=255)
                                                                                                                      ccAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTAt                     >  6:300458/1‑149 (MQ=255)
                                                                                                                      ccAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTAt                     <  1:242259/149‑1 (MQ=255)
                                                                                                                        aGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTc                                                                      <  2:204336/98‑1 (MQ=255)
                                                                                                                        aGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTc                                                                      >  1:204336/1‑98 (MQ=255)
                                                                                                                        aGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAaga                                >  2:126946/1‑136 (MQ=255)
                                                                                                                        aGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAaga                                <  1:126946/136‑1 (MQ=255)
                                                                                                                               tataaatgcaaGTTCGCTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGAt                                                >  7:69591/4‑113 (MQ=255)
                                                                                                                               tataaatgcaaGTTCGCTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGAt                                                <  8:69591/110‑1 (MQ=255)
                                                                                                                                  aaatgcaaGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAAc         >  5:333345/1‑149 (MQ=255)
                                                                                                                                    atgcaaGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGa       <  7:9501/149‑1 (MQ=255)
                                                                                                                                          gCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGCCtt  >  1:338203/1‑148 (MQ=255)
                                                                                                                                              |                                                                                                                                                
ATGTCGAGCGAGGAAGCTTGCTACGTTGCGTAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGCCTT  >  NC_002947/1748761‑1749046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑