Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,782,535	C→A	8.7%	Q514H (CAG→CAT) ‡	glnD ←	uridylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,782,535	0	C	A	8.7%	276.3 / 13.6	92	Q514H (CAG→CAT) ‡	glnD	uridylyltransferase
Reads supporting (aligned to +/- strand):  ref base C (41/43);  new base A (3/5);  total (44/48)
Fisher's exact test for biased strand distribution p-value = 7.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.09e-01

GTCGGACAAGTCCTTGCGCTGAGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTGCGCAGGTGCTTGATGAGGTTGAG  >  NC_002947/1782388‑1782681                                                                                                                                                    |                                                                                                                                                      gTCGGACAAGTCCTTGCGCTGAGCGGTGGTCGACATCACCAGGTGGTTCTGCACGAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCt                                                                                                                                                      <  3:102061/149‑1 (MQ=255)    ggACAAGTCCTTGCGCTGAGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTgg                                                                                                                                                    <  3:261265/148‑1 (MQ=255)        aaGTCCTTGCGCTGAGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGAcc                                                                                                                                               >  4:86448/1‑149 (MQ=255)          gTCCTTGCGCTGAGCGGTGGTCGACCTCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTACTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑t                                                                                                                                            >  8:134558/1‑149 (MQ=255)          gTCCTTCCGCTCAGCGGTGGTCCACATCACCAGGACCTTCTGCACCAGCCAGACGATCAGGCGGCTGTCACAGGCCGGCAACTTCAGCCGTTAGCAGAACTACTGACCGTCCACTCAACCGATTACCGACTGGTCGCCCTGGACAccc                                                                                                                                              >  8:139325/1‑148 (MQ=255)            ccTTGCGCTGAGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCAGGGCGAccact                                                                                                                                            <  4:3334/148‑4 (MQ=255)             cTTGCGCTGAGCGGTGGTCGACATCACCAGGTTGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCAGGGCGAccactcg                                                                                                                                          <  6:195269/149‑6 (MQ=255)             cTTGCGCTGAGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGc                                                                                                                                         <  3:86448/149‑1 (MQ=255)              ttGCGCTGAGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGcc                                                                                                                                        >  1:29246/1‑149 (MQ=255)              ttGCGCTGAGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGc                                                                                                                                         >  7:215480/1‑148 (MQ=255)              ttGCGCTGAGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCAAGACGATCAGGCGGCTGTCACAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGc                                                                                                                                         >  5:131316/1‑148 (MQ=255)                gcgcTGAGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGc                                                                                                                                         <  8:215480/146‑1 (MQ=255)                gcgcTGAGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGc                                                                                                                                         <  6:131316/146‑1 (MQ=255)                gcgcTGAGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGa                                                                                                                                      >  2:14337/1‑149 (MQ=255)                 cgcTGAGCGGTGCTCGACATCACCAGGGGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGAt                                                                                                                                     <  6:329323/149‑1 (MQ=255)                      aGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGt                                                                                                                                <  5:118251/149‑1 (MQ=255)                             gtCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACa                                                                                                                          <  1:161643/148‑1 (MQ=255)                             gtCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAg                                                                                                                         >  4:99634/1‑149 (MQ=255)                                 aCATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTGCCAGGCCGGCAACTGGTGCCGTTCGCAGCACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCg                                                                                                                     >  5:222285/1‑149 (MQ=255)                                  cATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCgg                                                                                                                    >  5:336019/1‑149 (MQ=255)                                        cAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGc                                                                                                                                                   >  6:77942/1‑113 (MQ=255)                                             ggtTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGAt                                                                                                          >  8:12771/1‑148 (MQ=255)                                                  tGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAg                                                                                                                >  5:146959/1‑137 (MQ=255)                                                  tGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAg                                                                                                                <  6:146959/137‑1 (MQ=255)                                                    cACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑Cgg                                                                                                 >  6:222730/1‑149 (MQ=255)                                                     aCCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCAGGGCGAccactcg                                                                                                                                          >  8:95888/1‑104 (MQ=255)                                                     aCCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCAGGGCGAccactcg                                                                                                                                          <  7:95888/109‑6 (MQ=255)                                                       cagccagACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGt                                                                                                              >  2:62769/1‑134 (MQ=255)                                                        agccagACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTTCCg                                                                                                                                       <  2:55324/108‑1 (MQ=255)                                                        agccagACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCg                                                                                                                                       >  1:55324/1‑108 (MQ=255)                                                          ccagACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGGCGTTAGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTg                                                                                            <  1:82672/149‑1 (MQ=255)                                                          ccagACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGc                                                                                                      >  1:346445/1‑139 (MQ=255)                                                          ccagACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGc                                                                                                      <  2:346445/139‑1 (MQ=255)                                                           cagACAATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTg                                                                                                                               >  7:272652/1‑113 (MQ=255)                                                           cagACAATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTg                                                                                                                               <  8:272652/113‑1 (MQ=255)                                                            agACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTggg                                                                                          >  5:252159/1‑148 (MQ=255)                                                            agACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTggg                                                                                          >  8:8106/1‑148 (MQ=255)                                                              aCGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAg                                                                                       <  6:336019/149‑1 (MQ=255)                                                                gATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGgc                                                                                     >  8:51940/1‑149 (MQ=255)                                                                  tCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGgcgc                                                                                   <  3:99634/149‑1 (MQ=255)                                                                    aGGCGGCTGTCCCAGGCCGGCAACTGGTGCCTTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGcc                                                                                  <  6:252159/148‑1 (MQ=255)                                                                         gcTGTTCCAGGCCGGCAACTGGTGCCGGTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑Ga                                                                           <  6:222285/149‑1 (MQ=255)                                                                           tGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCTGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGc                                                                         >  2:83551/1‑149 (MQ=255)                                                                               ccAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGc                                                                     >  3:310622/1‑149 (MQ=255)                                                                                cAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGGACCGATTTCCGAGTGGTCGCCCTGTCGACCC‑TTGCCTATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCt                                                                    <  2:238112/149‑1 (MQ=255)                                                                                cAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCt                                                                    <  7:12771/149‑1 (MQ=255)                                                                                         aaCTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCTGGCCAGCgg                                                           >  6:220874/1‑149 (MQ=255)                                                                                          aCTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCTGGCCAGCgg                                                           >  3:324341/1‑148 (MQ=255)                                                                                             ggTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGt                                                                                                              >  2:248144/1‑96 (MQ=255)                                                                                             ggTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGAAGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGt                                                                                                              <  1:248144/96‑1 (MQ=255)                                                                                              gTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGccc                                                                                 >  5:78954/1‑123 (MQ=255)                                                                                              gTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGccc                                                                                 <  6:78954/123‑1 (MQ=255)                                                                                              gTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAC‑GAGCTTGCTGGCCAGCGGGAAt                                                       <  7:51940/148‑1 (MQ=255)                                                                                               tGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCTGGCCAGCGGGAAttt                                                     <  5:222730/149‑1 (MQ=255)                                                                                                  cGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTGGCCCTGGGGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCTGGCCAGCGGGAATTtctc                                                  <  7:8106/149‑1 (MQ=255)                                                                                                   gTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCTGGCCAGCGGGAATTTCTCg                                                 >  1:321872/1‑149 (MQ=255)                                                                                                   gTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCTGGCCAGCGGGAATTTCTCg                                                 >  2:266360/1‑149 (MQ=255)                                                                                                    ttCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCTGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCCGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCTGGCCAGCGGGAATTTCTCg                                                 <  1:83551/149‑1 (MQ=255)                                                                                                      cGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCt                                                                    >  6:210506/1‑127 (MQ=255)                                                                                                      cGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCt                                                                    <  5:210506/127‑1 (MQ=255)                                                                                                       gCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTATATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCTg                                                                   >  7:235138/1‑127 (MQ=255)                                                                                                       gCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCTGGCCAGCGGGAATTTCTCg                                                 <  5:132074/145‑1 (MQ=255)                                                                                                       gCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCTGGCCAGCGGGAATTTCTCg                                                 >  6:132074/1‑145 (MQ=255)                                                                                                       gCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAAATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCTg                                                                   <  8:235138/127‑1 (MQ=255)                                                                                                         aGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGACGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCTGGCCAGCGGGAATTTCTCGGACAcc                                           >  5:334522/1‑149 (MQ=255)                                                                                                            aCTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGc                                        <  2:29246/149‑1 (MQ=255)                                                                                                              ttCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCgt                                      >  4:45218/1‑149 (MQ=255)                                                                                                                   cgcgTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCgtgt                                    <  7:111090/146‑1 (MQ=255)                                                                                                                    gcgTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCTGGCCAGCGGGAATTtctc                                                  >  6:91552/1‑131 (MQ=255)                                                                                                                    gcgTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCTGGCCAGCGGGAATTtctc                                                  <  5:91552/131‑1 (MQ=255)                                                                                                                    gcgTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTg                                <  2:321872/149‑1 (MQ=255)                                                                                                                      gTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCTGGCCAGCGGGAATTTCTCGGACACTGGCGTGTATTGCa                              <  5:220874/149‑1 (MQ=255)                                                                                                                      gTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCa                              <  4:310622/149‑1 (MQ=255)                                                                                                                      gTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCa                              <  4:324341/149‑1 (MQ=255)                                                                                                                       tCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAg                             >  7:40368/1‑149 (MQ=255)                                                                                                                          aCTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCtt                          >  8:150167/1‑149 (MQ=255)                                                                                                                              cACCGCTTTCCGAGTGGGCGCCCTGGCGTCCC‑TTGCCGTTGTCGTGGTAAAGCCCGGCCATGTAGATCAGCT‑GGGGTTTGGGCAGGCGCCCCATCGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTgc                        <  1:266360/148‑1 (MQ=255)                                                                                                                              cACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTgcg                       >  7:281392/1‑148 (MQ=255)                                                                                                                              cACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTgcg                       >  8:106813/1‑148 (MQ=255)                                                                                                                                gctaTTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGTGTTTGGGCAGGCGCCCCAT‑GAGCTTGCTGGCCAGCGGGAATTTCTCGTACACCGGCGTGTATTGCAGCTTGCGCAg                    <  7:139325/146‑1 (MQ=255)                                                                                                                                ccGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTGCGCAg                    <  7:134558/149‑1 (MQ=255)                                                                                                                                 cGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTGCGCAgg                   <  8:281392/149‑1 (MQ=255)                                                                                                                                        gcaagggTCGCCAGGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTGCGCAGGTGCt               <  5:282914/141‑1 (MQ=255)                                                                                                                                        gcaagggTCGCCAGGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTGCGCAGGTGCt               >  6:282914/6‑146 (MQ=255)                                                                                                                                        gcaagggTCGCCAGGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTGCGCAGGTGCt               <  5:278006/141‑1 (MQ=255)                                                                                                                                        gcaagggTCGCCAGGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTGCGCAGGTGCt               >  6:278006/6‑146 (MQ=255)                                                                                                                                         gAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTGCGCAGGTGCTtgat           <  6:334522/149‑1 (MQ=255)                                                                                                                                           gTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTGCGCAGGTGCTtgatga         <  1:14337/149‑1 (MQ=255)                                                                                                                                            tGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTGCGCAGGTGCTTGATGAg        >  2:175953/1‑149 (MQ=255)                                                                                                                                              gTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTGCGCAGGTGCTTGATGAGGt      <  2:170493/149‑1 (MQ=255)                                                                                                                                                 gCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTGCGCAGGTGCTtgat           >  2:320781/1‑141 (MQ=255)                                                                                                                                                 gCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTGCGCAGGTGCTtgat           <  1:320781/141‑1 (MQ=255)                                                                                                                                                  cccTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTGCGCAGGTGCTTGATGAGGTTGAg  <  1:175953/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                      GTCGGACAAGTCCTTGCGCTGAGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCC‑TTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCT‑CGGGTTTGGGCAGGCGCCCCAT‑GAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTGCGCAGGTGCTTGATGAGGTTGAG  >  NC_002947/1782388‑1782681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑