Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 45,680 C→G 18.2% intergenic (‑156/+47) cadA‑I ← / ← PP_0042 cadmium translocating P‑type ATPase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294745,6800CG18.2% 115.3 / 17.0 49intergenic (‑156/+47)cadA‑I/PP_0042cadmium translocating P‑type ATPase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (16/24);  new base G (4/5);  total (20/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTTCTGGGGTCAAGGCTTCCAATTGCCCTCTGCAGTTGGGGAGCCGTCTCTGTTGGTTGGCTAAGATCTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGAATACATCGAGTAACGAACAGTCTTGAGCGTGCCCTGGGAGTCCTCGAAGGTCATCAACCGTGGA  >  NC_002947/45533‑45814
                                                                                                                                                   |                                                                                                                                      
ccTTCTGGGGTCAAGGCTTCCAATTGCCCTCTGCAGTTGGGGAGCCGTCTCTGTTGGTTGGCTAAGATCTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACt                                                                                                                                       >  7:142341/1‑149 (MQ=255)
       gggTCAAGGCTTCCAATTGCCCTCTGCAGTTGGGGAGCCGTCTCTGTTGGTTGGCTAAGATCTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCt                                                                                                                                   <  8:142341/146‑1 (MQ=255)
         gTCAAGGCTTCCAATTGCCCTCTGCAGTTGGGGAGCCGTCTCTGTTGGTTGGCTAAGATCTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATTGGGTATCGGGCGTAATAAAAAATGCCAGGCAGTGCCTGGCAt                                                                                                                              <  8:230761/149‑1 (MQ=255)
         gTCAAGGATTCCAATTGCCCTCTGCAGTTGGGGAGCCGTCTCTGTTGGTTGGCTAAGATCTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCAt                                                                                                                              <  8:254773/149‑1 (MQ=255)
            aaGGCTTCCAATTGCCCTCTGCAGTTGGGGAGCCGTCTCTGTTGGTTGGCTAAGATCTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCAttt                                                                                                                            <  3:314829/148‑1 (MQ=255)
                 ttCCAATTGCCCTCTGCAGTTGGGGAGCCGTCTCTGTTGGTTGGCTAAGATCTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAAt                                                                                                                      >  4:152234/1‑149 (MQ=255)
                   ccAATTGCCCTCAGCAGTTGGGGAGCCGTCTCTTTTGGTTGGCTAAGATCTATTGCACTGTTTTCCCGAGCCCAGGCGTTAACATTTCGAATCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAAtt                                                                                                                     <  7:328648/148‑1 (MQ=255)
                        tGCCCTCTGCAGTTGGGGAGCCGTCTCTGTTGGTTGGCTAAGATCTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCAGTGCCTGGCAtt                                                                                                                             >  6:234865/1‑135 (MQ=255)
                        tGCCCTCTGCAGTTGGGGAGCCGTCTCTGTTGGTTGGCTAAGATCTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCAGTGCCTGGCAtt                                                                                                                             <  5:234865/135‑1 (MQ=255)
                               tGCAGTTGGGGAGCCGTCTCTGTTGGTTGGCTAAGATCTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATtgtt                                                                                                          <  8:22025/147‑1 (MQ=255)
                                  aGTTGGGGAGCCGTCTCTGTTGGTTGGCTAAGATCTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATtgttctgtt                                                                                                     <  1:328144/149‑1 (MQ=255)
                                    ttctggAGCCGTCTCTGTTGGTTGGCTAAGATCTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCa                                                                                                   <  7:43174/145‑1 (MQ=255)
                                      ggggAGCCGTCTCTGTTGGTTGGCTAAGATCTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATtgttcgacatcg                                                                                                  >  1:296192/1‑141 (MQ=255)
                                      ggggAGCCGTCTCTGTTGGTTGGCTAAGATCTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATtgttc                                                                                                         <  2:296192/141‑1 (MQ=255)
                                                     ttggttggCTAAGATCTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACttg                                                                                   <  3:177985/148‑1 (MQ=255)
                                                            gCTAAGATCTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGa                                                                            <  3:152234/148‑1 (MQ=255)
                                                            gCTAAGATCTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGAt                                                                           <  1:38594/149‑1 (MQ=255)
                                                                 gATCTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAGGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGAt                                                                           <  6:148330/144‑1 (MQ=255)
                                                                 gATCTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAGGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGAt                                                                           >  5:148330/1‑144 (MQ=255)
                                                                      aTTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCAGTGCCTGGCa                                                                                                                               >  5:18988/1‑87 (MQ=255)
                                                                      aTTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCAGTGCCTGGCa                                                                                                                               <  6:18988/87‑1 (MQ=255)
                                                                      aTTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGaa                                                                 >  5:145128/1‑149 (MQ=255)
                                                                       ttGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGc                                                                    >  6:280344/1‑145 (MQ=255)
                                                                       ttGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGc                                                                    <  5:280344/145‑1 (MQ=255)
                                                                         gCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGaa                                                                 >  6:248210/1‑146 (MQ=255)
                                                                         gCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGaa                                                                 <  5:248210/146‑1 (MQ=255)
                                                                          cACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGAATACa                                                             >  6:150540/1‑149 (MQ=255)
                                                                            cGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGAATACATc                                                           <  5:150540/149‑1 (MQ=255)
                                                                                   cccGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGaa                                                                 >  5:146098/1‑136 (MQ=255)
                                                                                   cccGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGaa                                                                 <  6:146098/136‑1 (MQ=255)
                                                                                            aGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGAATACATCGAGTAACGAACAGTCt                                           >  8:22529/1‑149 (MQ=255)
                                                                                             ggCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGAATACATCGAGTAACGAACAGTCtt                                          <  3:271228/149‑1 (MQ=255)
                                                                                               cGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGAATACATCGAGTAACGAACAGTCTTGa                                        <  6:145128/149‑1 (MQ=255)
                                                                                                 ttAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGAATACATCGAGTAACGAACAGTCTTGAGc                                      >  2:300230/1‑149 (MQ=255)
                                                                                                    aCATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGa                                                                            <  3:228229/108‑1 (MQ=255)
                                                                                                    aCATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGa                                                                            >  4:228229/1‑108 (MQ=255)
                                                                                                           gAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGAATACATCGAGTAACGAACAGTCTTGAGCGTGCCCTgg                             >  2:88686/1‑148 (MQ=255)
                                                                                                                tATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGAATACATCGAGTAACGAACAGTCTTGAGCGTGCCCTGGGa                           <  3:83726/145‑1 (MQ=255)
                                                                                                                tATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGAATACATCGAGTAACGAACAGTCTTGAGCGTGCCCTGGGa                           >  4:83726/1‑145 (MQ=255)
                                                                                                                     ggTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGAATACATCGAGTAACGAACAGTCTTGAGCGTGCCCTGGGAGTCCTCGaa                  <  1:300230/149‑1 (MQ=255)
                                                                                                                           gggCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGAATACATCGAGTAACGAACAGTCTTGAGCGTGCCCTGGGAGTCCTCGAAGGtcat            <  1:88686/149‑1 (MQ=255)
                                                                                                                                  ttcataaaTGCCAGGCAGTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGAATACATCGAGTAACGAACAGTCTTGAGc                                      >  1:192371/6‑116 (MQ=255)
                                                                                                                                  ttcataaaTGCCAGGCAGTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGAATACATCGAGTAACGAACAGTCTTGAGc                                      <  2:192371/111‑1 (MQ=255)
                                                                                                                                   taaAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGAATACATCGAGTAACGAACAGTCTTGAGCGTGCCCTGGGAGTCCTCGAAGGTCATCAACCGTg    <  7:22529/149‑1 (MQ=255)
                                                                                                                                       aaaTGCCAGGCAGTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGAATACATCGAGTAACGAACAGTCTTGAg                                       >  4:239667/1‑110 (MQ=255)
                                                                                                                                       aaaTGCCAGGCAGTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGAATACATCGAGTAACGAACAGTCTTGAg                                       <  3:239667/110‑1 (MQ=255)
                                                                                                                                             cAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGAATACATCGAGTAACGAACAGTCTTGAGCGTGCCCTGGGa                           >  4:21627/1‑116 (MQ=255)
                                                                                                                                                  aCTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGAATACATCGAGTAACGAACAGTCTTGAGCGTGCCCTGGGAGTCCTCGAAGGTCATCAACCGTGGa  <  4:186966/136‑1 (MQ=255)
                                                                                                                                                  aCTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGAATACATCGAGTAACGAACAGTCTTGAGCGTGCCCTGGGAGTCCTCGAAGGTCATCAACCGTGGa  >  3:186966/1‑136 (MQ=255)
                                                                                                                                                   |                                                                                                                                      
CCTTCTGGGGTCAAGGCTTCCAATTGCCCTCTGCAGTTGGGGAGCCGTCTCTGTTGGTTGGCTAAGATCTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGAATACATCGAGTAACGAACAGTCTTGAGCGTGCCCTGGGAGTCCTCGAAGGTCATCAACCGTGGA  >  NC_002947/45533‑45814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑