| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 196,879 | G→A | 24.2% | V795V (GTG→GTA) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 196,879 | 0 | G | A | 24.2% | 51.1 / 13.3 | 33 | V795V (GTG→GTA) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base G (11/14); new base A (3/5); total (14/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01 | |||||||||||
GTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACC‑ACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCG‑TGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGT > NC_002947/196739‑196937 | gTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACC‑ACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCG‑TGAt < 8:265645/143‑1 (MQ=32)gTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACC‑ACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCG‑TGAt > 7:265645/1‑143 (MQ=32) ccATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACC‑ACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCG‑TGATCACCTACAcc > 1:19548/1‑149 (MQ=32) ccATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACC‑ACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACAGAAGGCGGCG‑TGATCACCTACAcc < 2:301813/149‑1 (MQ=17) cATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCGACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCG‑TGATCACCTACAcc > 1:104247/1‑149 (MQ=17) cATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACC‑ACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCG‑TGATCAc < 8:150536/141‑1 (MQ=32) cATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACC‑ACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCG‑TGATCAc > 7:150536/1‑141 (MQ=32) cATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACC‑ACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCG‑TGATCACCTACACCg > 3:23622/1‑149 (MQ=32) tCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACC‑ACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCG‑TGATCa < 8:206960/138‑1 (MQ=32) tCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACC‑ACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCG‑TGATCa > 7:206960/1‑138 (MQ=32) agaACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACC‑ACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCG‑TGATCACCTACACCGTGAcc < 8:62248/149‑1 (MQ=33) aCGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACC‑ACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCG‑TGATCACCTACACCGTGACCCTg < 7:26703/149‑1 (MQ=34) cGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACC‑ACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCG‑TGATCACCTACACCGTGACCCTGa < 4:23622/149‑1 (MQ=34) cGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACC‑ACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCG‑TGATCACCTACACCGTGACCCTGa < 5:131260/149‑1 (MQ=34) tGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACC‑ACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCG‑TGATCACCTACACCGt > 8:292768/1‑135 (MQ=11) tGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACC‑ACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCG‑TGATCACCTACACCGt < 7:292768/135‑1 (MQ=11) tttCGAACACCTGACCCCGAACCCGACCCCGGCTCAGACC‑ACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCG‑TGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGa < 6:245746/148‑1 (MQ=255) cTTCGAACAGGTGACCCCGAACCCGACGCCGGCTCAGACC‑ACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGCTGATCACCTACACCGTGACCCTGAGCAATc < 1:207346/141‑1 (MQ=12) cTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACC‑ACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCG‑TGATCACCTACACCGTGACCCTGAGCAATc > 2:207346/1‑140 (MQ=32) gAACAGCTGACCCCGAACCCGACGCCGGCTCAGACC‑ACGATCAACGACTCGGTCGATGACACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCG‑TGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGcc > 3:148725/1‑149 (MQ=17) aaCAGCTGACCCCGAACCCGACGCCGGCTCAGACC‑ACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCG‑TGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCg > 6:170623/1‑149 (MQ=32) gCTGACCCCGAACCCGACGCCGGCTCAGACC‑ACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCG‑TGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGa < 4:148725/149‑1 (MQ=32) cgggccccGCACCCGACGCCGGCGCAGACC‑ACGCACAACCACTAGGGAGATTCCCCCCCCGCGACCCTGACGGCGAGCCCGTGGGTAACCGCAGGCGGCG‑TGGTCACCTAAACCCGGAAACGGAGGAATCCAGCCCACCCGCCCGTGAc > 6:254028/5‑149 (MQ=2) aCCCCGAACCCGACGCCGGCTCAGACC‑ACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCG‑TGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACa < 8:300309/147‑1 (MQ=32) aCCCCGAACCCGACCCCGGCTCAGACC‑ACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCG‑TAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACa < 2:212182/147‑1 (MQ=11) ccccGAACCCGACCCCGGCTCAGACC‑ACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCG‑TAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTg < 4:223996/149‑1 (MQ=17) ccccGAACCCGACCCCGGCTCAGACC‑ACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCG‑TAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCAGTGACAGTg < 2:171958/149‑1 (MQ=12) cccGAACCCGACCCCGGCTCAGACC‑ACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCG‑TAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGa > 7:27549/1‑149 (MQ=17) cccGAACCCGACCCCGGCTCAGACC‑ACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAACGCGGCG‑TAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGa > 4:256107/1‑149 (MQ=12) cGAACCCGACGCCGGCTCAGACC‑ACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCG‑TGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGAcc < 4:14587/149‑1 (MQ=32) cGAACCCGACCCCGGCTCAGACC‑ACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCG‑TAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGAcc > 6:276291/1‑149 (MQ=17) cGAACCCGACCCCGGCTCAGACC‑ACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCG‑TAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGAcc < 6:37075/149‑1 (MQ=17) cccGACCCCGGCTCAGACC‑ACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCG‑TAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGt < 5:276291/149‑1 (MQ=11) | GTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACC‑ACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCG‑TGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGT > NC_002947/196739‑196937 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |