Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,809,176 G→T 7.6% R76L (CGT→CTT)  eno → enolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,809,1760GT 7.6% 202.9 / 6.5 66R76L (CGT→CTT) enoenolase
Reads supporting (aligned to +/- strand):  ref base G (31/29);  new base T (2/3);  total (34/32)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.48e-01

CTCGACAACGGCATCATCGGCAGCGCTTGCGCGCCGTCCGGTGCTTCTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAGCTGCTGCCCAGG  >  NC_002947/1809028‑1809322
                                                                                                                                                    |                                                                                                                                                  
cTCGACAACGGCATCATCGGCAGCGCTTGCGCGCCGTCCGGTGCTTCTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCg                                                                                                                                                    <  2:171079/149‑1 (MQ=255)
   gACAACGGCATCATCGGCAGCGCTTGCGCGCCGTCCGGTGCTTCTACCGGCTAGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGa                                                                                                                                                 >  8:150927/1‑149 (MQ=255)
         ggCATCATCGGCAGCGCTTGCGCGCCGTCCGGTGCTTCTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCCACGGCCCGATCCGTGACctgct                                                                                                                                           >  4:240827/1‑149 (MQ=255)
               atcGGCAGCGCTTGCGCGCCGTCCGGTGCTTCTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCTTGCCCAGCAGCAggt                                                                                                                                     >  6:219311/1‑148 (MQ=255)
                  ggCAGCGCTTGCGCGCCGTCCGGTGCTTCTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGc                                                                                                                                    >  5:290985/1‑147 (MQ=255)
                   gCAGCGCTTGCGCGCCGTCCGGTGCTTCTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCTTGCCCAGCAGCAGGTCacg                                                                                                                                 <  5:219311/149‑3 (MQ=255)
                       cgcTTGCGCGCCGTCCGGTGCTTCTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCTTGCCCAGCAGCAGGTCACGGATc                                                                                                                             <  1:167548/149‑1 (MQ=255)
                       cgcTTGCGCGCCGTCCGGTGCTTCTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCTTGCCCAGCAGCAGGTCACGGATc                                                                                                                             >  3:81773/1‑149 (MQ=255)
                       cgcTTGCGCGCCGTCCGGTGCTTCTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATc                                                                                                                             <  1:271889/149‑1 (MQ=255)
                         cTTGCGCGCCGTCCGGTGCTTCTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCTTGCCCAGCAGCAGGTCACGGATcgg                                                                                                                           <  4:81773/149‑3 (MQ=255)
                          ttGCGCGCCGTCCGGTGCTTCTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCtt                                                                                                                          >  7:187082/1‑149 (MQ=255)
                            gcgcgcCGTCCGGTGCTTCTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCtt                                                                                                                          <  3:34123/147‑1 (MQ=255)
                                      cGGTGCTTCTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAgg                                                                                                              >  7:172819/1‑149 (MQ=255)
                                        gTGCTTCTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACctgctgctg                                                                                                                                       >  4:119683/1‑122 (MQ=255)
                                         tGCTTCTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGctc                                                                                                           <  8:187082/149‑1 (MQ=255)
                                           cTTCTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTg                                                                                                         <  6:290985/149‑1 (MQ=255)
                                           cTTCTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTg                                                                                                         >  4:174037/1‑149 (MQ=255)
                                           cTTCTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGAAGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCCTGACCTGCTGCTGGGCAAGGATCCTTCCGAACAGAAGGCTCTg                                                                                                         >  4:248849/1‑149 (MQ=255)
                                              cTACCGTCTCGCGCGAAGCGCTGGAGCTGCGTTATGGCGAAAAGAGCCTTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCTATCCGTGACCTGCTGCTTGGCAAGGATCCTTCCGATCAGAATGctcttg                                                                                                        <  4:154196/147‑3 (MQ=255)
                                                aCCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACcg                                                                                                    >  2:96100/1‑149 (MQ=255)
                                                aCCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACcg                                                                                                    >  3:79111/1‑149 (MQ=255)
                                                 ccGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGAc                                                                                                      <  3:174037/146‑1 (MQ=255)
                                                 ccGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACcgc                                                                                                   <  4:79111/149‑1 (MQ=255)
                                                        gcgcgAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGa                                                                                            >  5:135565/1‑149 (MQ=255)
                                                         cgcgAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGTCCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGAt                                                                                           <  1:202149/149‑1 (MQ=255)
                                                          gcgAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCa                                                                                               <  6:355967/144‑1 (MQ=255)
                                                          gcgAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCa                                                                                               >  5:355967/1‑144 (MQ=255)
                                                           cgAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATc                                                                                          >  3:310027/1‑148 (MQ=255)
                                                              aGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAAc                                                                                      >  1:71712/1‑149 (MQ=255)
                                                                cgcTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTg                                                                                    <  5:148704/149‑1 (MQ=255)
                                                                     gAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCAGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACgg                                                                               >  6:66752/1‑149 (MQ=255)
                                                                            gTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCgag                                                                        <  4:310027/149‑1 (MQ=255)
                                                                                    gACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGc                                                                <  7:150927/149‑1 (MQ=255)
                                                                                      cAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGcc                                                               >  1:9761/1‑148 (MQ=255)
                                                                                       aaGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGcc                                                               >  5:123002/1‑147 (MQ=255)
                                                                                         gagCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCg                                                                          <  3:49355/134‑1 (MQ=255)
                                                                                         gagCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCg                                                                          >  4:49355/1‑134 (MQ=255)
                                                                                          agCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAg                                                                  <  1:145779/141‑1 (MQ=255)
                                                                                          agCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAg                                                                  >  2:145779/1‑141 (MQ=255)
                                                                                            ccGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAAc                                                                                      >  6:80331/1‑119 (MQ=255)
                                                                                            ccGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAAc                                                                                      <  5:80331/119‑1 (MQ=255)
                                                                                                      ggCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGgc                                                      <  5:349688/141‑1 (MQ=255)
                                                                                                      ggCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGgc                                                      >  6:349688/1‑141 (MQ=255)
                                                                                                      ggCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACg                                               <  2:9761/148‑1 (MQ=255)
                                                                                                           gggCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAg                                                            >  3:220213/1‑130 (MQ=255)
                                                                                                           gggCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAg                                                            <  4:220213/130‑1 (MQ=255)
                                                                                                            ggCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGTCGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCt                                        <  6:123002/149‑1 (MQ=255)
                                                                                                                tGCTGAAGGCCGTCGCCAACATTAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCt                                    >  7:283096/1‑149 (MQ=255)
                                                                                                                   tGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGc                                                                >  4:142235/1‑118 (MQ=255)
                                                                                                                   tGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGtt                                 >  7:84797/1‑149 (MQ=255)
                                                                                                                   tGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGtt                                 >  1:23058/1‑149 (MQ=255)
                                                                                                                   tGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGtt                                 >  1:254770/1‑149 (MQ=255)
                                                                                                                     aaGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGtt                                 <  5:66752/147‑1 (MQ=255)
                                                                                                                         ccGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCt                            <  2:193804/148‑1 (MQ=255)
                                                                                                                         ccGTCGCCAACATCAACGGCCCGATCCGAGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCt                            >  1:193804/1‑148 (MQ=255)
                                                                                                                          cGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTgg                          <  3:240827/149‑1 (MQ=255)
                                                                                                                           gTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGc                         <  8:84797/149‑1 (MQ=255)
                                                                                                                           gTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGc                         <  2:254770/149‑1 (MQ=255)
                                                                                                                                 aaCATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTgccgc                   >  5:229198/1‑149 (MQ=255)
                                                                                                                                   cATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTg                                       >  6:144776/1‑127 (MQ=255)
                                                                                                                                   cATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTg                                       <  5:144776/127‑1 (MQ=255)
                                                                                                                                    aTCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCa                 >  1:326293/1‑148 (MQ=255)
                                                                                                                                       aaCGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAg              <  8:283096/148‑1 (MQ=255)
                                                                                                                                                aTCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAGCTGCTGCcca    <  6:229198/149‑1 (MQ=255)
                                                                                                                                                  ccGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAGCTGCTGccc     <  2:83451/146‑1 (MQ=255)
                                                                                                                                                  ccGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAGCTGCTGccc     >  1:83451/1‑146 (MQ=255)
                                                                                                                                                   cGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAGCTGCTGCccagg  <  3:248849/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CTCGACAACGGCATCATCGGCAGCGCTTGCGCGCCGTCCGGTGCTTCTACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGCCAACATCAACGGCCCGATCCGTGACCTGCTGCTGGGCAAGGATCCTTCCGATCAGAAGGCTCTGGACCGCGCCATGATCGAACTGGACGGTACCGAGAACAAGGCCAAGCTGGGCGCCAACGCCATCCTGGCTGTTTCCCTGGCTGCCGCCAAAGCTGCTGCCCAGG  >  NC_002947/1809028‑1809322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑