Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,812,302 G→C 7.8% A39G (GCC→GGC) ‡ PP_1615 ← LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,812,3020GC 7.8% 156.3 / 4.8 51A39G (GCC→GGC) ‡PP_1615LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (26/21);  new base C (2/2);  total (28/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

CAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGC‑TGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCGATTATCCCTGTGTGGCAATAATGTTT  >  NC_002947/1812157‑1812445
                                                                                                                                                   |                                                                                                                                                
cAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGC‑TGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGa                                                                                                                                               >  2:5430/1‑149 (MQ=255)
      gCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGC‑TGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGc                                                                                                                                          <  1:5430/148‑1 (MQ=255)
        ccATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGC‑TGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCgg                                                                                                                                       <  8:301091/149‑1 (MQ=255)
                        ttCCGCACGGCCATCCTGCAAGCGTTGGCAATGG‑TGCAGGAAGGGCTGGCCGGCTTCGCTCAGGGGTACCCGGCGGGTGCGGCGAAACAGCAGGCGGGTTTGCAGGCGGTCCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAg                                                                                                                        <  1:238377/149‑1 (MQ=255)
                              aCGGCCATCCTGCAAGCGTTGGCAATGC‑TGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAAc                                                                                                                   <  8:10167/147‑1 (MQ=255)
                                ggCCATCCTGCAAGCGTTGGCAATGC‑TGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAAccccc                                                                                                               <  2:145107/149‑1 (MQ=255)
                                   cATCCTGCAAGCGTTGGCAATGC‑TGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAgg                                                                                                            >  6:20576/1‑149 (MQ=255)
                                      ccTGCAAGCGTTGGCAATGC‑TGCAGTAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGAGTGTGCTGCGATACATCAGGCGCGTTTGCAGGCGGT‑AATCCATACGTGAGATCTGACGGATGATGTGAGATGATGAAACCCCCAGGctt                                                                                                         >  4:46191/1‑147 (MQ=255)
                                      ccTGCAAGCGTTGGCAATGC‑TGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGt                                                                                                         >  8:220996/1‑149 (MQ=255)
                                       cTGCAAGCGTTGGCAATGC‑TGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGGGATCTGCCGGCt                                                                                                                                     >  5:304248/1‑120 (MQ=255)
                                       cTGCAAGCGTTGGCAATGC‑TGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCt                                                                                                                                     >  1:69133/1‑120 (MQ=255)
                                       cTGCAAGCGTTGGCAATGC‑TGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCt                                                                                                                                     <  2:69133/120‑1 (MQ=255)
                                       cTGCAAGCGTTGGCAATGC‑TGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCt                                                                                                                                     <  6:304248/120‑1 (MQ=255)
                                        ctgcaGCATTGCCAACGCTTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGtt                                                                                                        >  1:171945/5‑149 (MQ=255)
                                           aaGCGTTGGCAATGC‑TGCAGGAAGGTCTGCCCGGCTTCACTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAg                                                                                                             >  5:256234/1‑140 (MQ=255)
                                           aaGCGTTGGCAATGC‑TGCAGGAAGGTCTGCCCGGCTTCACTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAg                                                                                                             <  6:256234/140‑1 (MQ=255)
                                                     aaTGC‑TGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAACCCCCCAGGCGTTCGGCGGCGGCAGt                                                                                           >  4:184363/1‑148 (MQ=255)
                                                     aaTGC‑TGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGt                                                                                           >  6:236936/1‑148 (MQ=255)
                                                     aaTGC‑TGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGt                                                                                           >  6:320218/1‑148 (MQ=255)
                                                             catgAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGTCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGCT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGAGGCGGCAGTGAACTGGc                                                                                   <  3:46191/146‑1 (MQ=255)
                                                             cAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGAtgtg                                                                                                                               <  6:221542/105‑1 (MQ=255)
                                                             cAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGAtgtg                                                                                                                               >  5:221542/1‑105 (MQ=255)
                                                             cAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGc                                                                                   <  7:220996/149‑1 (MQ=255)
                                                                 aaGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGa                                                                               <  5:20576/149‑1 (MQ=255)
                                                                     tCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCg                                                                           >  8:59483/1‑149 (MQ=255)
                                                                            ggCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTc                                                                            >  8:280364/1‑141 (MQ=255)
                                                                            ggCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTc                                                                            <  7:280364/141‑1 (MQ=255)
                                                                                   cTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACaaa                                                            >  4:19302/1‑149 (MQ=255)
                                                                                       gggTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCTGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATtcg                                                        >  6:279683/1‑149 (MQ=255)
                                                                                              ccGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGc                                                 >  5:132500/1‑149 (MQ=255)
                                                                                              ccGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGc                                                 >  3:251179/1‑149 (MQ=255)
                                                                                                    ggTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGCCTTc                                             >  5:306627/1‑147 (MQ=255)
                                                                                                    ggTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGCCTTc                                             <  6:306627/147‑1 (MQ=255)
                                                                                                         tgcGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGCCTTCCCATCgg                                      >  1:187355/1‑149 (MQ=255)
                                                                                                         tgcGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGCCTTCCCATCgg                                      >  5:299481/1‑149 (MQ=255)
                                                                                                            gATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGCCTTCCCATCGgctg                                   <  4:251179/149‑1 (MQ=255)
                                                                                                             ttACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGCCTTCCCATCGgctgc                                  >  4:148840/2‑149 (MQ=255)
                                                                                                                 agcagGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑c                                                                        >  6:293861/1‑107 (MQ=255)
                                                                                                                     ggCGCGTTTGCAGGCGGT‑CCTCCATCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCg                            <  2:301375/147‑1 (MQ=255)
                                                                                                                     ggCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGGCCCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCGa                           <  1:72815/149‑1 (MQ=255)
                                                                                                                     ggCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCg                            >  1:301375/1‑147 (MQ=255)
                                                                                                                         cgTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCGATTATc                      <  7:59483/149‑1 (MQ=255)
                                                                                                                          gTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCGATTATcc                     <  6:299481/149‑1 (MQ=255)
                                                                                                                              gCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCGATTATCCCtgt                 >  8:272731/1‑149 (MQ=255)
                                                                                                                               cAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCGATTATCCCtgt                 <  5:236936/148‑1 (MQ=255)
                                                                                                                               cAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCGATTATCCCtgt                 <  5:320218/148‑1 (MQ=255)
                                                                                                                                          atcAGCCGGCAGATCGCCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCGATTATCCCTGTGTGGCaataat      >  4:285722/3‑149 (MQ=255)
                                                                                                                                            cAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATc                             >  4:181192/1‑124 (MQ=255)
                                                                                                                                            cAGCCGGCAGATCGCCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCGATTATCCCTGTGTGGCAATAATGt    >  8:193685/1‑149 (MQ=255)
                                                                                                                                             aGCCGGCAGATCGCCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCGATTATCCCTGTGTGGCAATAATGtt   <  3:285722/149‑1 (MQ=255)
                                                                                                                                              gCCGGCAGATCGCCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCGATTATCCCTGTGTGGCAATAATGttt  <  7:193685/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CAGGTCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGC‑TGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCGATTATCCCTGTGTGGCAATAATGTTT  >  NC_002947/1812157‑1812445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑