Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,812,308 T→G 8.0% Q37P (CAG→CCG) ‡ PP_1615 ← LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,812,3080TG 8.0% 149.2 / 4.8 50Q37P (CAG→CCG) ‡PP_1615LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (25/21);  new base G (2/2);  total (27/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

GCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGC‑TGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCGATTATCCCTGTGTGGCAATAATGTTT  >  NC_002947/1812163‑1812445
                                                                                                                                                   |                                                                                                                                          
gCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGC‑TGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGc                                                                                                                                          <  1:5430/148‑1 (MQ=255)
  ccATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGC‑TGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCgg                                                                                                                                       <  8:301091/149‑1 (MQ=255)
                  ttCCGCACGGCCATCCTGCAAGCGTTGGCAATGG‑TGCAGGAAGGGCTGGCCGGCTTCGCTCAGGGGTACCCGGCGGGTGCGGCGAAACAGCAGGCGGGTTTGCAGGCGGTCCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAg                                                                                                                        <  1:238377/149‑1 (MQ=255)
                        aCGGCCATCCTGCAAGCGTTGGCAATGC‑TGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAAc                                                                                                                   <  8:10167/147‑1 (MQ=255)
                          ggCCATCCTGCAAGCGTTGGCAATGC‑TGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAAccccc                                                                                                               <  2:145107/149‑1 (MQ=255)
                             cATCCTGCAAGCGTTGGCAATGC‑TGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAgg                                                                                                            >  6:20576/1‑149 (MQ=255)
                                ccTGCAAGCGTTGGCAATGC‑TGCAGTAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGAGTGTGCTGCGATACATCAGGCGCGTTTGCAGGCGGT‑AATCCATACGTGAGATCTGACGGATGATGTGAGATGATGAAACCCCCAGGctt                                                                                                         >  4:46191/1‑147 (MQ=255)
                                ccTGCAAGCGTTGGCAATGC‑TGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGt                                                                                                         >  8:220996/1‑149 (MQ=255)
                                 cTGCAAGCGTTGGCAATGC‑TGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGGGATCTGCCGGCt                                                                                                                                     >  5:304248/1‑120 (MQ=255)
                                 cTGCAAGCGTTGGCAATGC‑TGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCt                                                                                                                                     <  6:304248/120‑1 (MQ=255)
                                 cTGCAAGCGTTGGCAATGC‑TGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCt                                                                                                                                     >  1:69133/1‑120 (MQ=255)
                                 cTGCAAGCGTTGGCAATGC‑TGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCt                                                                                                                                     <  2:69133/120‑1 (MQ=255)
                                  ctgcaGCATTGCCAACGCTTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGtt                                                                                                        >  1:171945/5‑149 (MQ=255)
                                     aaGCGTTGGCAATGC‑TGCAGGAAGGTCTGCCCGGCTTCACTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAg                                                                                                             <  6:256234/140‑1 (MQ=255)
                                     aaGCGTTGGCAATGC‑TGCAGGAAGGTCTGCCCGGCTTCACTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAg                                                                                                             >  5:256234/1‑140 (MQ=255)
                                               aaTGC‑TGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAACCCCCCAGGCGTTCGGCGGCGGCAGt                                                                                           >  4:184363/1‑148 (MQ=255)
                                               aaTGC‑TGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGt                                                                                           >  6:320218/1‑148 (MQ=255)
                                               aaTGC‑TGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGt                                                                                           >  6:236936/1‑148 (MQ=255)
                                                       catgAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGTCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGCT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGAGGCGGCAGTGAACTGGc                                                                                   <  3:46191/146‑1 (MQ=255)
                                                       cAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGAtgtg                                                                                                                               <  6:221542/105‑1 (MQ=255)
                                                       cAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGAtgtg                                                                                                                               >  5:221542/1‑105 (MQ=255)
                                                       cAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGc                                                                                   <  7:220996/149‑1 (MQ=255)
                                                           aaGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGa                                                                               <  5:20576/149‑1 (MQ=255)
                                                               tCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCg                                                                           >  8:59483/1‑149 (MQ=255)
                                                                      ggCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTc                                                                            <  7:280364/141‑1 (MQ=255)
                                                                      ggCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTc                                                                            >  8:280364/1‑141 (MQ=255)
                                                                             cTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACaaa                                                            >  4:19302/1‑149 (MQ=255)
                                                                                 gggTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCTGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATtcg                                                        >  6:279683/1‑149 (MQ=255)
                                                                                        ccGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGc                                                 >  5:132500/1‑149 (MQ=255)
                                                                                        ccGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGc                                                 >  3:251179/1‑149 (MQ=255)
                                                                                              ggTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGCCTTc                                             <  6:306627/147‑1 (MQ=255)
                                                                                              ggTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGCCTTc                                             >  5:306627/1‑147 (MQ=255)
                                                                                                   tgcGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGCCTTCCCATCgg                                      >  5:299481/1‑149 (MQ=255)
                                                                                                   tgcGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGCCTTCCCATCgg                                      >  1:187355/1‑149 (MQ=255)
                                                                                                      gATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGCCTTCCCATCGgctg                                   <  4:251179/149‑1 (MQ=255)
                                                                                                       ttACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGCCTTCCCATCGgctgc                                  >  4:148840/2‑149 (MQ=255)
                                                                                                           agcagGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑c                                                                        >  6:293861/1‑107 (MQ=255)
                                                                                                               ggCGCGTTTGCAGGCGGT‑CCTCCATCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCg                            <  2:301375/147‑1 (MQ=255)
                                                                                                               ggCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGGCCCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCGa                           <  1:72815/149‑1 (MQ=255)
                                                                                                               ggCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCg                            >  1:301375/1‑147 (MQ=255)
                                                                                                                   cgTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCGATTATc                      <  7:59483/149‑1 (MQ=255)
                                                                                                                    gTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCGATTATcc                     <  6:299481/149‑1 (MQ=255)
                                                                                                                        gCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCGATTATCCCtgt                 >  8:272731/1‑149 (MQ=255)
                                                                                                                         cAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCGATTATCCCtgt                 <  5:236936/148‑1 (MQ=255)
                                                                                                                         cAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCGATTATCCCtgt                 <  5:320218/148‑1 (MQ=255)
                                                                                                                                    atcAGCCGGCAGATCGCCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCGATTATCCCTGTGTGGCaataat      >  4:285722/3‑149 (MQ=255)
                                                                                                                                      cAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATc                             >  4:181192/1‑124 (MQ=255)
                                                                                                                                      cAGCCGGCAGATCGCCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCGATTATCCCTGTGTGGCAATAATGt    >  8:193685/1‑149 (MQ=255)
                                                                                                                                       aGCCGGCAGATCGCCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCGATTATCCCTGTGTGGCAATAATGtt   <  3:285722/149‑1 (MQ=255)
                                                                                                                                        gCCGGCAGATCGCCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCGATTATCCCTGTGTGGCAATAATGttt  <  7:193685/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                          
GCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGC‑TGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGT‑CCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGATTCGG‑CCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCGATTATCCCTGTGTGGCAATAATGTTT  >  NC_002947/1812163‑1812445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑