Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,859,399 T→A 6.0% T4S (ACT→TCT) ‡ PP_1662 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,859,3990TA 6.0% 206.1 / 2.8 66T4S (ACT→TCT) ‡PP_1662hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (31/31);  new base A (2/2);  total (33/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CCCACCAGTTGACCTTGTTGGTACACATCCCAGATCCGCACGCCAGCGGCCAGGCCTTCGTGGGACATTCTCGATTCGTCGCGTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCA  >  NC_002947/1859260‑1859542
                                                                                                                                           |                                                                                                                                               
cccACCAGTTGACCTTGTTGGTACACATCCCAGATCCGCACGCCAGCGGCCAGGCCTTCGTGGGACATTCTCGATTCGTCGCGTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACACACTGTCGCag                                                                                                                                        <  8:277165/149‑2 (MQ=255)
 ccaccaGTTGACCTTGTTGGTACACATCCCAGATCCGCACGCCAGCGGCCAGGCCTTCGTGGGACATTCTCGATTCGTCGCGTTCGGTTACTTGGTCAGTCAACGGTAAACTCCACTTCTTCAAGATGTCTGCGACAGTGTGTCGCACg                                                                                                                                       >  1:283913/1‑149 (MQ=255)
 ccaccaGTTGACCTTGTTGGTACACATCCCAGATCCGCACGCCAGCGGCCAGGCCTTCGTGGGACATTCTCGATTCGTCGCGTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACg                                                                                                                                       >  6:41818/1‑149 (MQ=255)
  caccaGTTGACCTTGTTGGTACACATCCCAGATCCGCACGCCAGCGGCCAGGCCTTCGTGGGACATTCTCGATTCGTCGCGTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGt                                                                                                                                      <  7:280506/149‑1 (MQ=255)
    ccaGTTGACCTTGTTGGTACACATCCCAGATCCGCACGCCAGCGGCCAGGCCTTCGTGGGACATTCTCGATTCGTCGCGTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGtct                                                                                                                                    <  2:170715/149‑1 (MQ=255)
     caGTTGACCTTGTTGGTACACATCCCAGATCCGCACGCCAGCGGCCAGGCCTTCGTGGGACATTCTCGATTCGTCGCGTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAgtgtg                                                                                                                                              >  4:215354/1‑138 (MQ=255)
     caGTTGACCTTGTTGGTACACATCCCAGATCCGCACGCCAGCGGCCAGGCCTTCGTGGGACATTCTCGATTCGTCGCGTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAgtgtg                                                                                                                                              <  3:215354/138‑1 (MQ=255)
     caGTTGACCTTGTTGGTACACATCCCAGATCCGCACGCCAGCGGCCAGGCCTTCGTGGGACATTCTCGATTCGTCGCGTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCAc                                                                                                                                        >  4:17533/1‑144 (MQ=255)
     caGTTGACCTTGTTGGTACACATCCCAGATCCGCACGCCAGCGGCCAGGCCTTCGTGGGACATTCTCGATTCGTCGCGTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCAc                                                                                                                                        <  3:17533/144‑1 (MQ=255)
       gTTGACCTTGTTGGTACACATCCCAGATCCGCACGCCAGCGGCCAGGCCTTCGTGGGACATTCTCGATTCGTCGCGTTCGGTTACTTGGTCAGTCATCGGTAAACTCCACTTCTTGAAGATGGCTGCGAGAGTGTGTCGCAGGtcttct                                                                                                                                 >  8:117391/1‑149 (MQ=255)
       gTTGACCTTGTTGGTACACATCCCAGATCCGCACGCCAGCGGCCAGGCCTTCGTGGGACATTCTCGATTCGTCGCGTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGtcttct                                                                                                                                 >  3:329786/1‑149 (MQ=255)
        ttGACCTTGTTGGTACACATCCCAGATCCGCACGCCAGCGGCCAGGCCTTCGTGGGACATTCTCGATTCGTCGCGTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTcttctt                                                                                                                                <  5:41818/149‑1 (MQ=255)
              ttgttgGTACACATCCCAGATCCGCACGCCAGCGGCCAGGCCTTCGTGGGACATTCTCGATTCGTCGCGTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTta                                                                                                                              >  5:302904/1‑145 (MQ=255)
                   ggTACACATCCCAGATCCGCACGCCAGCGGCCAGGCCTTCGTGGGACATTCTCGATTCGTCGCGTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGttt                                                                                                                      <  1:147396/148‑1 (MQ=255)
                    gTACACATCCCAGATCCGCACGCCAGCGGCCAGGCCTTCGTGGGACATTCTCGATTCGTCGCGTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATaga                                                                                                                          <  2:256695/143‑1 (MQ=255)
                    gTACACATCCCAGATCCGCACGCCAGCGGCCAGGCCTTCGTGGGACATTCTCGATTCGTCGCGTTCGGTTACTTGGACAGTCAACGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATaga                                                                                                                          >  1:256695/1‑143 (MQ=255)
                    gTACACATCCCAGATCCGCACGCCAGCGGCCAGGCCTTCGTGGGACATTCTCGATTCGTCGCGTTAGGTTACTTGGAAAGTCATCGGTAAACTCCACTTCTTAAAGATGGCTGCGACAGTGTGTCGCACGTCTTATTTATAGAGTTTga                                                                                                                    >  2:312306/1‑148 (MQ=255)
                       cacaTCCCAGATCCGCACGCCAGCGGCCAGGCCTTCGTGGGACATTCTCGATTCGTCGCGTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTa                                                                                                                  <  7:117391/148‑1 (MQ=255)
                        acaTCCCAGATCCGCACGCCAGCGGCCAGGCCTTCGTGGGACATTCTCGATTCGTCGCGTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAg                                                                                                                 >  6:154271/1‑148 (MQ=255)
                          aTCCCAGATCCGCACGCCAGCGGCCAGGCCTTCGTGGGACATTCTCGATTCGTCGCGTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAg                                                                                                                 >  5:283775/1‑146 (MQ=255)
                             ccAGATCCGCACGCCAGCGGCCAGGCCTTCGTGGGACATTCTCGATTCGTCGCGTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACt                                                                                                           <  6:302904/149‑1 (MQ=255)
                                     gCACGCCAGCGGCCAGGCCTTCGTGGGACATTCTCGATTCGTCGCGTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCa                                                                                                               >  3:260461/1‑137 (MQ=255)
                                     gCACGCCAGCGGCCAGGCCTTCGTGGGACATTCTCGATTCGTCGCGTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCa                                                                                                               <  4:260461/137‑1 (MQ=255)
                                              cGGCCAGGCCTTCGTGGGACCTTCGCGATTCGTCGCGTTCGGGTACTTGGACAGGCATCGGTAAACTCCACATCTTCAAGATGGCTGGGTCAGTGTGTCGGACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGc                                                                                          <  4:65052/149‑1 (MQ=255)
                                                 ccAGGCCTTCGTGGGACATTCTCGATTCGTCGCGTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCAt                                                                                        >  6:139681/1‑148 (MQ=255)
                                                  cAGGCCTTCGTGGGACATTCTCGATTCGTCGCGTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACtaagtaa                                                                                                     <  7:86367/134‑1 (MQ=255)
                                                  cAGGCCTTCGTGGGACATTCTCGATTCGTCGCGTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACtaagtaa                                                                                                     >  8:86367/1‑134 (MQ=255)
                                                  cAGGCCTTCGTGGGACATTCTCGATTCGTCGCGTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAgg                                                                                           >  3:263253/1‑144 (MQ=255)
                                                  cAGGCCTTCGTGGGACATTCTCGATTCGTCGCGTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAgg                                                                                           <  4:263253/144‑1 (MQ=255)
                                                    ggCCTTCGTGGGACATTCTCGATTCGTCGCGTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGaaa                                                                                    <  6:283775/149‑1 (MQ=255)
                                                    ggCCTTCGTGGGACATTCTCGATTCGTCGCGTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGaaa                                                                                    <  4:329786/149‑1 (MQ=255)
                                                       cTTCGTGGGACATTCTCGATTCGTCGCGTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACaa                                                                                 <  5:139681/149‑1 (MQ=255)
                                                       cTTCGTGGGACATTCTCGATTCGTCGCGTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACaa                                                                                 >  5:240791/1‑149 (MQ=255)
                                                                  aTTCTCGATTCGTCGCGTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAg                                                                      <  5:154271/149‑1 (MQ=255)
                                                                      tcGATTCGTCGCGTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCa                                                                                                               <  2:117780/104‑1 (MQ=255)
                                                                      tcGATTCGTCGCGTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACAAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTc                                                                   >  5:303972/1‑148 (MQ=255)
                                                                              tcgCGTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGAtg                                                          >  4:337868/1‑149 (MQ=255)
                                                                                  gTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCa                                                                                         <  1:196341/114‑1 (MQ=255)
                                                                                  gTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCa                                                                                         >  2:196341/1‑114 (MQ=255)
                                                                                     cGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACTTGTTTCATgc                                                                        >  1:216918/1‑127 (MQ=255)
                                                                                     cGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACTTGTTTCATgc                                                                        <  2:216918/128‑2 (MQ=255)
                                                                                      ggTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCtgg                                                  >  5:37401/1‑149 (MQ=255)
                                                                                       gTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCtggg                                                 >  6:118888/1‑149 (MQ=255)
                                                                                           cTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCtgggtgg                                              <  5:118888/148‑1 (MQ=255)
                                                                                            ttGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAggg                                                             <  4:132907/132‑1 (MQ=255)
                                                                                            ttGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAggg                                                             >  3:132907/1‑132 (MQ=255)
                                                                                              ggACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGc                                            <  2:283913/147‑1 (MQ=255)
                                                                                              ggAAAGGCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGAGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGCCTACGTAAGTAAAACAGGCATGAAACAAGTTTCATGACGCTCGAAGGGATGCTGCCTGGGTGGGCtt                                          >  6:397/1‑149 (MQ=255)
                                                                                                aCAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGAt                                                           >  7:188325/1‑130 (MQ=255)
                                                                                                aCAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGAt                                                           <  8:188325/130‑1 (MQ=255)
                                                                                                 cAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACTAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCtttt                                        <  6:240791/148‑1 (MQ=255)
                                                                                                  aGTCATCGGTAAAGTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGGTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTc                                      <  1:312306/149‑1 (MQ=255)
                                                                                                     cATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGaaa                                                                                    <  8:53135/100‑1 (MQ=255)
                                                                                                     cATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGaaa                                                                                    >  7:53135/1‑100 (MQ=255)
                                                                                                      aTCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCtgggt                                                >  7:15688/1‑135 (MQ=255)
                                                                                                      aTCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCtgggt                                                <  8:15688/135‑1 (MQ=255)
                                                                                                          gTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCaaa                              <  3:337868/149‑1 (MQ=255)
                                                                                                          gTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCaaa                              <  6:303972/149‑1 (MQ=255)
                                                                                                            aaaCTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAg                             >  3:36114/1‑148 (MQ=255)
                                                                                                                tCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAg                        >  1:84920/1‑149 (MQ=255)
                                                                                                                   aCTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGccc                     >  8:30828/1‑149 (MQ=255)
                                                                                                                        ttcAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCAc                >  3:76563/1‑149 (MQ=255)
                                                                                                                           aaGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCGGCAAAGAAAAGCCCCGCACTgg             >  3:180410/1‑149 (MQ=255)
                                                                                                                               gacgtGCGACACACTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCgca                                   >  4:343199/5‑123 (MQ=255)
                                                                                                                               gacgtGCGACACACTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCgca                                   <  3:343199/119‑1 (MQ=255)
                                                                                                                                 cgtGCGACACACTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCgggg       >  2:237708/3‑149 (MQ=255)
                                                                                                                                      gACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCa  <  2:84920/149‑1 (MQ=255)
                                                                                                                                      gACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCa  <  4:36114/149‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                               
CCCACCAGTTGACCTTGTTGGTACACATCCCAGATCCGCACGCCAGCGGCCAGGCCTTCGTGGGACATTCTCGATTCGTCGCGTTCGGTTACTTGGACAGTCATCGGTAAACTCCACTTCTTCAAGATGGCTGCGACAGTGTGTCGCACGTCTTCTTTATAGAGTTTGTTAGCAGACTAAGTAAGTAAAACAGGCATGAAACAAGTTTCATGAAGCTCGAAGGGATGCTGCCTGGGTGGGCTTTTTCGCAGCAAAGAAAAGCCCCGCACTGGGCGGGGCTTCA  >  NC_002947/1859260‑1859542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑