Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,876,964 G→A 7.5% R260H (CGC→CAC)  PP_1682 → glycoside hydrolase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,876,9640GA 7.5% 149.6 / 2.0 53R260H (CGC→CAC) PP_1682glycoside hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base G (24/25);  new base A (2/2);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.57e-01

GATACGCCGATCATGGTCGATGCCGGCTGGTATGCAGGCGCAGACGCGTTCGGCTACTGGCCGGCGCCGCTGGAAGACCCGCGAGTGCTGTACAGCGTGCACATGTATGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGACACAGCTGTGGGATGGGCGGCGAGTTGAAGGCTACTTGCGCCAGCCGCTGGCATGGGCCGAGGCCATGGGGCTGCCGCTTTCTCGTCTGGTGGTGGGC  >  NC_002947/1876819‑1877109
                                                                                                                                                  |                                                                                                                                                 
gATACGCCGATCATGGTCGATGCCGGCTGGTATGCAGGCGCAGACGCGTTCGGCTACTGGCCGGCGCCGCTGGAAGACCCGCGAGTGCTGTACAGCGTGCACATGTATGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCa                                                                                                                                                 >  8:271738/1‑148 (MQ=255)
             tGGTCGATGCCGGCTGGTATGCAGGCGCAGACGCGTTCGGCTACTGGCCGGCGCCGCTGGAAGACCCGCGAGTGCTGTACAGCGTGCACATGTATGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCg                                                                                                                                     <  4:49713/147‑1 (MQ=255)
             tGGTCGATGCCGGCTGGTATGCAGGCGCAGACGCGTTCGGCTACTGGCCGGCGCCGCTGGAAGACCCGCGAGTGCTGTACAGCGTGCACATGTATGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCg                                                                                                                                     >  3:49713/1‑147 (MQ=255)
                  gATGCCGGCTGGTATGCAGGCGCAGACGCGTTCGGCTACTGGCCGGCGCCGCTGGAAGACCCGCGAGTGCTGTACAGCGTGCACATGTATGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATc                                                                                                                               <  4:171527/148‑1 (MQ=255)
                   aTGCCGGCTGGTATGCAGGCGCAGACGCGTTCGGCTACTGGCCGGCGCCGCTGGAAGACCCGCGAGTGCTGTACAGCGTGCACATGTATGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATc                                                                                                                               >  7:287626/1‑147 (MQ=255)
                         gCTGGTATGCAGGCGCAGACGCGTTCGGCTACTGGCCGGCGCCGCTGGAAGACCCGCGAGTGCTGTACAGCGTGCACATGTATGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGc                                                                                                                                    >  1:18879/1‑136 (MQ=255)
                         gCTGGTATGCAGGCGCAGACGCGTTCGGCTACTGGCCGGCGCCGCTGGAAGACCCGCGAGTGCTGTACAGCGTGCACATGTATGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGc                                                                                                                                    <  2:18879/136‑1 (MQ=255)
                           tGGTATGCAGGCGCAGACGCGTTCGGCTACTGGCCGGCGCCGCTGGAAGACCCGCGAGTGCTGTACAGCGTGCACATGTATGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAGGCCCCg                                                                                                                      >  3:286618/1‑148 (MQ=255)
                             gTATGCAGGCGCAGACGCGTTCGGCTACTGGCCGGCGCCGCTGGAAGACCCGCGAGTGCTGTACAGCGTGCACATGTATGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAg                                                                                                                            >  7:202104/1‑140 (MQ=255)
                             gTATGCAGGCGCAGACGCGTTCGGCTACTGGCCGGCGCCGCTGGAAGACCCGCGAGTGCTGTACAGCGTGCACATGTATGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAg                                                                                                                            <  8:202104/140‑1 (MQ=255)
                               aTGCAGGCGCAGACGCGTTCGGCTACTGGCCGGCGCCGCTGGAAGACCCGCGAGTGCTGTACAGCGTGCACATGTATGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAGGCCCCGTATCt                                                                                                                 >  1:330302/1‑149 (MQ=255)
                                   aGGCGCAGACGCGTTCGGCTACTGGCCGGCGCCGCTGGAAGACCCGCGAGTGCTGTACAGCGTGCACATGTATGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTg                                                                                                             >  7:90375/1‑149 (MQ=255)
                                             gcgTTCGGCTACTGGCCGGCGCCGCTGGAAGACCCGCGAGTGCTGTACAGCGTGCACATGTATGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGac                                                                                                   <  8:276967/149‑1 (MQ=255)
                                              cgTTCGGCTACTGGCCGGCGCCGCTGGAAGACCCGCGAGTGCTGTACAGCGTGCACATGTATGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGaca                                                                                                  <  4:286618/149‑1 (MQ=255)
                                                     cTACTGGCCGGCGCCGCTGGAAGACCCGCGAGTGCTGTACAGCGTGCACATGTATGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGACACAGCtgt                                                                                           <  3:304787/149‑1 (MQ=255)
                                                        cTGGCCGGCGCCGCTGGAAGACCCGCGAGTGCTGTACAGCGTGCACATGTATGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGACACAGCTGTggg                                                                                        <  2:330302/149‑1 (MQ=255)
                                                            ccGGCGCCGCTGGAAGACCCGCGAGTGCTGTACAGCGTGCACATGTATGAGACCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGACACAGCTGTGGGATgg                                                                                    <  8:287626/149‑1 (MQ=255)
                                                                 gccgcTGGAAGACCCGCGAGTGCTGTACAGCGTGCACATGTATGAGCCCTAATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCCGACACAGCTGTGGGATGggcgg                                                                                >  1:261852/1‑149 (MQ=255)
                                                                  ccgcTGGAAGACCCGCGTGTACTGTACAGCGTGCACATGTATGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGGAAAGAGCCCATCGCCTATCCTGGCCCCGTATCTTTTGCCGGGCAGAGACAGCTGTGGGATGGgctgcg                                                                              <  8:160706/149‑1 (MQ=255)
                                                                  ccgcTGGAAGACCCGCGAGTGCTGTACAGCGTGCACATGTATGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAGGCCCCGTATCt                                                                                                                 >  2:25445/1‑114 (MQ=255)
                                                                  ccgcTGGAAGACCCGCGAGTGCTGTACAGCGTGCACATGTATGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAGGCCCCGTATCt                                                                                                                 <  1:25445/114‑1 (MQ=255)
                                                                        gAAGACCCGCGAGTGCTGTACAGCGTGCACATGTATGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGACACAGCTGTGGGATGGGCGGCGAGtt                                                                          <  8:90375/147‑1 (MQ=255)
                                                                        gAAGACCCGCGAGTGCTGTACAGCGTGCACATGTATGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGACACAGCTGTGGGATGGGCGGCGAGTTg                                                                         >  2:7154/1‑148 (MQ=255)
                                                                         aaGACCCGCGAGTGCTGTACAGCGTGCACATGTATGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGACACAGCTGTGGGATGGGCGGCGAGTTGaa                                                                       <  7:271738/149‑1 (MQ=255)
                                                                              ccGCGAGTGCTGTACAGCGTGCACATGTATGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGACACAGCTGTGGGATGGGCGGCGAGtt                                                                          >  1:156370/1‑141 (MQ=255)
                                                                              ccGCGAGTGCTGTACAGCGTGCACATGTATGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGACACAGCTGTGGGATGGGCGGCGAGtt                                                                          <  2:156370/141‑1 (MQ=255)
                                                                              ccGCGAGTGCTGTACAGCGTGCACATGTATGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGACACAGCTGTGGGATGGGCGGCGAGTTGAAGGCTa                                                                  >  6:339712/1‑149 (MQ=255)
                                                                               cgcgAGTGCTGTACAGCGTGCACATGTATGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGACACAGCTGTGGGATGGGCGGCGAGTTGAAGGCTAc                                                                 <  6:124109/149‑1 (MQ=255)
                                                                                     tGCTGTACAGCGTGCACATGTATGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGACACAGCTGTGGGATGGGCGGCGAGTTGAAGGCTACTTgcgc                                                           <  2:261852/149‑1 (MQ=255)
                                                                                          tACAGCGTGCACATGTATGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGACACAGCTGTGGGATGGGCGGCGAGTTGAAGGCTACTTGCGCCAGcc                                                      >  6:304792/1‑149 (MQ=255)
                                                                                                   cacaTGTATGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGACACAGCtgtg                                                                                          <  6:229375/104‑1 (MQ=255)
                                                                                                   cacaTGTATGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGACACAGCtgtg                                                                                          >  5:229375/1‑104 (MQ=255)
                                                                                                    acaTGTATGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGACACAGCTGTGGGATGGGCGGCGAGTTGAAgg                                                                     >  6:115735/1‑124 (MQ=255)
                                                                                                     caTGTATGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGACACAGCTGTGGGATGGGCGGCGAGTTGAAGGCTACTTGCGCCAGCCGCTGGCATggg                                           >  6:227288/1‑149 (MQ=255)
                                                                                                      aTGTATGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGACACAGCTGTGGGATGGGCGGCGAGTTGaa                                                                       >  6:26099/1‑120 (MQ=255)
                                                                                                      aTGTATGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGACACAGCTGTGGGATGGGCGGCGAGTTGaa                                                                       <  5:26099/120‑1 (MQ=255)
                                                                                                        gTATGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGACACAGCTGTGGGATGGGCGGCGAGTTGaa                                                                       <  7:211831/118‑1 (MQ=255)
                                                                                                        gTATGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGACACAGCTGTGGGATGGGCGGCGAGTTGaa                                                                       >  8:206447/1‑118 (MQ=255)
                                                                                                        gTATGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGACACAGCTGTGGGATGGGCGGCGAGTTGaa                                                                       >  8:211831/1‑118 (MQ=255)
                                                                                                        gTATGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGACACAGCTGTGGGATGGGCGGCGAGTTGaa                                                                       <  7:206447/118‑1 (MQ=255)
                                                                                                         tATGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGACACAGCTGTGGGATGGGCGGCGAGTTGAAGGCTACTTGCGCCAGCCGCTGGCATGGGcc                                         >  5:279553/1‑147 (MQ=255)
                                                                                                           tGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGACACAGCTGTGGGATGGGCGGCGAGTTGAAGGCTACTTGCGCCAGCCGCTGGCATGGGCCGAgg                                     <  1:7154/149‑1 (MQ=255)
                                                                                                             aGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGACACAGCTGTGGGATGGGCGGCGAGTTGAAGGCTACTTGCGCCAGCCGCTGGCATGGGCCGAGGcc                                   <  5:227288/149‑1 (MQ=255)
                                                                                                                  t‑ATGCCGCAACCAGTGCGCCGAACATGGCGCACAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGACACAGCTGTGGGATGGGCGGCGAGTTGAAGGCTACTTGCGCCAGCCGCTGGCATGGGCCGAGGCCATgg                               >  7:120549/1‑148 (MQ=255)
                                                                                                                    aTGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGACACAGCTGTGGGATGGGCGGCGAGTTGAAGGCTACTTGCGCCAGCCGCTGGCATGGGCCGAGGCCATgggg                             >  8:276940/1‑149 (MQ=255)
                                                                                                                     tGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGACACAGCTGTGGGAGGGGCGGCGAGTTGAAGGCTACTTGCGCCAGCCGCTGGCATGGGCCGAGGCCATgggg                             <  3:21828/148‑1 (MQ=255)
                                                                                                                     tGCCGCAACCAGTGCGCCGAACATGGCGCACAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGACACAGCTGTGGGATGGGCGGCGAGTTGAAGGCTACTTGCGCCAGCCGCTGGCATGGGCCGAGGCCATgg                               <  8:120549/146‑1 (MQ=255)
                                                                                                                        cGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGACACAGCTGTGGGATGGGCGGCGAGTTGAAGGCTACTTGCGCCAGCCGCTGGCATGGGCCGa                                       <  6:157239/135‑1 (MQ=255)
                                                                                                                             ccAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGACACAGCTGTGGGATGGGCGGCGAGTTGAAGGCTACTTGCGCCAGCCGCTGGCATGGGCCGAGGCCATGGGGCTGCCGCtt                    >  2:225618/1‑149 (MQ=255)
                                                                                                                                    gcCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGACACAGCTGTGGGATGGGCGGCGAGTTGAAGGCTACTTGCGCCAGCCGCTGGCATGGGCCGAGGCCATGGGGCTGCCGCTTTCTCGTc             >  7:281212/1‑149 (MQ=255)
                                                                                                                                       gAACATGGCGCGCAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGACACAGCTGTGGGATGGGCGGCGAGTTGAAGGCTACTTGCGCCAGCCGCTGGCATGGGCCGAGGCCATGGGGCTGCCGCTTTCTCGTCtgg          <  5:304792/149‑1 (MQ=255)
                                                                                                                                         gttcggCGCACAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGACACAGCTGTGGGATGGGCGGCGAGTTGAAGGCTACTTGCGCCAGCCGCTGGCATgg                                            <  6:307617/109‑1 (MQ=255)
                                                                                                                                         gttcggCGCACAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGACACAGCTGTGGGATGGGCGGCGAGTTGAAGGCTACTTGCGCCAGCCGCTGCCATgg                                            >  5:307617/5‑113 (MQ=255)
                                                                                                                                               cgcgcAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTTCCGGGCAGACACAGCTGTGGGATGGGCGGCGAGTTGAAGGCTACTTGCGCCAGCCGCTGGCATGGGCCGAGGCCATGGGGCTGCCGCTTTCTCGTCTGGTGGTGGGc  <  7:82564/149‑1 (MQ=255)
                                                                                                                                               cgcgcAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGACACCGCTGTGGGATGGGCGGCGAGTTGAAGGCTACTTGCGCCAGCCGCTGGCATGGGCCGAGGCCATGGGGCTGCCGCTTTCTCGTCTGGTGGTGGGc  <  7:82557/149‑1 (MQ=255)
                                                                                                                                                gcgcAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGACACAGCTGTGGGATGGGCGGCGAGTTGAAGGCTACTTGCGCCAGCCGCTGGCATGGGCCGAGGCCATgggg                             >  8:102433/1‑121 (MQ=255)
                                                                                                                                                gcgcAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGACACAGCTGTGGGATGGGCGGCGAGTTGAAGGCTACTTGCGCCAGCCGCTGGCATGGGCCGAGGCCATgggg                             <  7:102433/121‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GATACGCCGATCATGGTCGATGCCGGCTGGTATGCAGGCGCAGACGCGTTCGGCTACTGGCCGGCGCCGCTGGAAGACCCGCGAGTGCTGTACAGCGTGCACATGTATGAGCCCT‑ATGCCGCAACCAGTGCGCCGAACATGGCGCGCAAACAGCCCATCGCCTATCCAGGCCCCGTATCTTTTGCCGGGCAGACACAGCTGTGGGATGGGCGGCGAGTTGAAGGCTACTTGCGCCAGCCGCTGGCATGGGCCGAGGCCATGGGGCTGCCGCTTTCTCGTCTGGTGGTGGGC  >  NC_002947/1876819‑1877109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑