Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,915,826 A→T 10.9% intergenic (+254/‑35) fklB‑II → / → PP_1715 FKBP‑type peptidyl‑prolyl cis‑trans isomerase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,915,8260AT10.9% 123.7 / 6.4 46intergenic (+254/‑35)fklB‑II/PP_1715FKBP‑type peptidyl‑prolyl cis‑trans isomerase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (22/19);  new base T (2/3);  total (24/22)
Fisher's exact test for biased strand distribution p-value = 6.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01

TTCTGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGCGTGAAACGACTGTGTAAAAAACGCCCGATCTGACCGGGCCCCTTGCCTGGCAGGCACCTGGGGGCGGAAACGTCGCCCTGTTCACAAGGTTATCCACAATAAATGTGGATAACCTTTTCCTCTCGGAGGCTCCATGAGCGCACCCTGGAATTTTGCCCGTTTCCTGCCATTGGCCGAGCGCCTGCTCAGCCGCGGTCGTTTGCCGGCCTTGTTGTTCGCGGTGGCGCGCAAAGGCCCGCGCCT  >  NC_002947/1915690‑1915970
                                                                                                                                        |                                                                                                                                                
ttCTGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGCGTGAAACGACTGTGTAAAAAACGCCCGATCTGACCGGGCCCCTTGCCTGGCAGGCACCTGGGGGCGGAAACGTCGCCCTGTTCACAAGGTTATCCACAATAAAtgt                                                                                                                                           >  4:35412/1‑144 (MQ=255)
ttCTGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGCGTGAAACGACTGTGTAAAAAACGCCCGATCTGACCGGGCCCCTTGCCTGGCAGGCACCTGGGGGCGGAAACGTCGCCCTGTTCACAAGGTTATCCACAATAAAtgt                                                                                                                                           <  3:35412/144‑1 (MQ=255)
 tCTGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGCGTGAAACGACTGTGTAAAAAACGCCCGATCTGACCGGGCCCCTTGCCTGGCAGGCACCTGGGGGCGGAAACGTCGCCCTGTTCACAAGGTTATCCACATTTATTGTGGATa                                                                                                                                      <  8:182589/148‑1 (MQ=255)
   tGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGCGTGAAACGACTGTGTAAAAAACGCCCGATCTGACCGGGCCCCTTGCCTGGCAGGCACCTGGGGGCGGAAACGTCGCCCTGTTCACAAGGTTATCCACAATAAATGTGGATAAcc                                                                                                                                   >  7:226551/1‑149 (MQ=255)
     aCGCAAATCGTTTGCGCCGCAGGTAGTCATGGCGTGAAACGACTGTGTAAAAAACGCCCGATCTGACCGGGCCCCTTGCCTGGCAGGCACCTGGGGGCGGAAACGTCGCCCTGTTCACAAGGTTATCCACATTTATTGTGGATAACCtt                                                                                                                                 <  1:341554/149‑1 (MQ=255)
        cAAATCGTTTGCGCCGCAGGTAGTCATGGCGTGAAACGACTGTGTTTTTTTCGCCCGATCTGACCGGGCCCCTTGCCTGGCAGGCACCTGGGGGCGGAAACGTCGCCCTGTTCACAAGGTTATCCACAATAAATGTGGATAACCTTTTc                                                                                                                              <  1:115772/149‑1 (MQ=255)
                 tGCGCCGCAGGTAGTCATGGCGTGAAACGACTGTGTAAAAAACGCCCGATCTGACCGGGCCCCTTGCCTGGCAGGCACCTGGGGGCGGAAACGTCGCCCTGTTCACAAGGTTATCCACAATAAATGTGGATAACCTTTTCCTCTCGGa                                                                                                                      <  8:226551/148‑1 (MQ=255)
                      cgcAGGTAGTCATTGCGTGAAACGACTGTGTAAAAACCCGCCGATCAGACCGGGCCCCTTGCCTGGGAGGCACCTGGGGGCGGAAACGTCGCCCTGTTCACAAGGTTATCCACAATAAATGTGGATAACCTTTTCCTCTCGGAGGCTcc                                                                                                                <  1:323217/149‑1 (MQ=255)
                      cgcAGGTAGTCATGGCGTGAAACGACTGTGTAAAAAACGCCCGATCTGACCGGGCCCCTTGCCTGGCAGGCACCTGGGGGCGGAAACGTCGCCCTGTTCACAAGGTTATCCACATTTATTGTGGATAACCtt                                                                                                                                 <  4:61492/132‑1 (MQ=255)
                      cgcAGGTAGTCATGGCGTGAAACGACTGTGTAAAAAACGCCCGATCTGACCGGGCCCCTTGCCTGGCAGGCACCTGGGGGCGGAAACGTCGCCCTGTTCACAAGGTTATCCACATTTATTGTGGATAACCtt                                                                                                                                 >  3:61492/1‑132 (MQ=255)
                      agcAGGTAGTCATGGCGTGAAACGACTGTGTAAAAAACGCCCGATCTGACCGGGCCCCTTGCCTGGCAGGCACCTGGGGGCGGAAACGTCGCCCTGTTCACAAGGTTATCCACAATAAATGTGGATAACCTTTTCCTCTCGGAGGCTcc                                                                                                                <  1:123599/148‑1 (MQ=255)
                       gcAGGTAGTCATGGCGTGAAACGACTGTGTAAAAAACGCCCGATCTGACCGGGCCCCTTGCCTGGCAGGCACCTGGGGGCGGAAACGTCGCCCTGTTCACAAGGTTATCCACAATAAATGTGGATAACCTTTTCCTCTCGGAGGCTcc                                                                                                                <  1:79349/148‑1 (MQ=255)
                       gcAGGTAGTCATGGCGTGAAACGACTGTGTAAAAAACGCCCGATCTGACCGGGCCCCTTGCCTGGCAGGCACCTGGGGGCGGAAACGTCGCCCTGTTCACAAGGTTATCCACAATAAATGTGGATAACCTTTTCCTCTCGGAGGCTcc                                                                                                                >  2:79349/1‑148 (MQ=255)
                                      gTGAAACGACTGTGTAAAAAACGCCCGATCTGACCGGGCCCCTTGCCTGGCAGGCACCTGGGGGCGGAAACGTCGCCCTGTTCACAAGGTTATCCACAATAAATGTGGAt                                                                                                                                       <  1:307206/110‑1 (MQ=255)
                                      gTGAAACGACTGTGTAAAAAACGCCCGATCTGACCGGGCCCCTTGCCTGGCAGGCACCTGGGGGCGGAAACGTCGCCCTGTTCACAAGGTTATCCACAATAAATGTGGAt                                                                                                                                       >  2:307206/1‑110 (MQ=255)
                                         aaaCGACTGTGTAAAAAACGCCCGATCTGACCGGGCCCCTTGCCTGGCAGGCACCTGGGGGCGGAAACGTCGCCCTGTTCACAAGGTTATCCACAATAAATGTGGATAACCTTTTCCTCTCGGAGGCTCCATGAGCGCACCCTGGAAtt                                                                                             >  4:274120/1‑149 (MQ=255)
                                          aaCGACTGTGTAAAAAACGCCCGATCTGACCGGGCCCCTTGCCTGGCAGGCACCTGGGGGCGGAAACGTCGCCCTGTTCACAAGGTTATCCACAATAAATGTGGATAACCtttt                                                                                                                               <  3:67810/114‑1 (MQ=255)
                                          aaCGACTGTGTAAAAAACGCCCGATCTGACCGGGCCCCTTGCCTGGCAGGCACCTGGGGGCGGAAACGTCGCCCTGTTCACAAGGTTATCCACAATAAATGTGGATAACCtttt                                                                                                                               >  4:67810/1‑114 (MQ=255)
                                           aCGACTGTGTAAAAAACGCCCGATCTGACCGGGCCCCTTGCCTGGCAGGCACCTGGGGGCGGAAACGTCGCCCTGTTCACAAGGTTATCCACAATAAATGTGGATAACCTTTTCCTCTCGGAGGCTCCATGAGCGCACCCTGGAAtttt                                                                                           <  4:106669/149‑1 (MQ=255)
                                             gACTGTGTAAAAAACGCCCGATCTGACCGGGCCCCTTGCCTGGCAGGCACCTGGGGGCGGAAACGTCGCCCTGTTCACAAGGTTATCCACAATAAATGTGGATAACCTTTTCCTCTCGGAGGCTCCATGAGCGCACCCTGGAATTTTGc                                                                                         >  3:6293/1‑149 (MQ=255)
                                                     aaaaaaCGCCCGATCTGACCGGGCCCCTTGCCTGGCAGGCACCTGGGGGCGGAAACGTCGCCCTGTTCACAAGGTTATCCACAATAAATGTGGATAACCTTTTCCTCTCGGAGGCTCCATGAGCGCACCCTGGAATTTTGCCCGTTTcc                                                                                 >  7:273959/1‑149 (MQ=255)
                                                       aaggggCCCGGTCAGATCGGGCCCCTTGCCTGGCAGGCACCTGGGGGCGGAAACGTCGCCCTGTTCACAAGGTTATCCACAATAAATGTGGATAACCTTTTCCTCTCGGAGGCTCCATGAGCGCACCCTGGAATTTTGCCCGTTTCCTg                                                                               >  7:111317/6‑149 (MQ=255)
                                                           ggCCCGGTCAGATCGGGCCCCTTGCCTGGCAGGCACCTGGGGGCGGAAACGTCGCCCTGTTCACAAGGTTATCCACAATAAATGTGGATAACCTTTTCCTCTCGGAGGCTCCATGAGCGCACCCTGGAATTTTGCCCGTTTCCTGCCAt                                                                           >  6:138911/2‑149 (MQ=255)
                                                                    agatcGGGCCCCTTGCCTGGCAGGCACCTGGGGGCGGAAACGTCGCCCTGTTCACAAGGTTATCCACAATAAATGTGGATAACCTTTTCCTCTCGGAGGCTCCATGAGCGCACCCTGGAATTTTGCCCGTTTCCTGCCATTGGCCGAgc                                                                  <  5:138911/145‑1 (MQ=255)
                                                                         gggCCCCTTGCCTGGCAGGCACCTGGGGGCGGAAACGTCGCCCTGTTCACAAGGTTATCCACAATAAATGTGGATAACCTTTTCCTCTCGGAGGCTCCATGAGCGCACCCTGGAATTTTGCCCGTTTCCTGCCATTGGCCGAGCGCCTg                                                             >  6:178421/1‑149 (MQ=255)
                                                                             cccTTGCCTGGCAGGCACCTGGGGGCGGAAACGTCGCCCTGTTCACAAGGTTATCCACAATAAATGTGGATAACCTTTTCCTCTCGGAGGCTCCATGAGCGCACCCTGGAATTTTGCCCGTTTCCTGCCATTGGCCGAGCGCCTGCt                                                           >  3:239688/1‑147 (MQ=255)
                                                                                ttGCCTGGCAGGCACCTGGGGGCGGAAACGTCGCCCTGTTCACAAGGTTATCCACAATAAATGTGGATAACCTTTTCCTCTCGGAGGCTCCATGAGCGCACCCTGGAATTTTGCCCGTTTCCTGCCATTGGCCGa                                                                    >  3:43492/1‑135 (MQ=255)
                                                                                ttGCCTGGCAGGCACCTGGGGGCGGAAACGTCGCCCTGTTCACAAGGTTATCCACAATAAATGTGGATAACCTTTTCCTCTCGGAGGCTCCATGAGCGCACCCTGGAATTTTGCCCGTTTCCTGCCATTGGCCGa                                                                    <  4:48047/135‑1 (MQ=255)
                                                                                ttGCCTGGCAGGCACCTGGGGGCGGAAACGTCGCCCTGTTCACAAGGTTATCCACAATAAATGTGGATAACCTTTTCCTCTCGGAGGCTCCATGAGCGCACCCTGGAATTTTGCCCGTTTCCTGCCATTGGCCGa                                                                    <  4:43492/135‑1 (MQ=255)
                                                                                ttGCCTGGCAGGCACCTGGGGGCGGAAACGTCGCCCTGTTCACAAGGTTATCCACAATAAATGTGGATAACCTTTTCCTCTCGGAGGCTCCATGAGCGCACCCTGGAATTTTGCCCGTTTCCTGCCATTGGCCGa                                                                    >  3:48047/1‑135 (MQ=255)
                                                                                  gCCTGGCAGGCACCTGGGGGCGGAAACGTCGCCCTGTTCACAAGGTTATCCACAATAAATGTGGATAACCTTTTCCTCTCGGAGGCTCCATGAGCGCACCCTGGAATTTTGCCCGTTTCCTGCCATTGGCCGAGCGCCTGCTCAGcc                                                      <  8:273959/147‑1 (MQ=255)
                                                                                         aggcaCCTGGGTGCGGAAACGTCGCCCTGTTCACAAGGTTATCAACAATAAATGTGGATAACCTTTTCCTCTCGGAGGCTCAATGAGCGCACCCTGGAATTTTGCCCGTTTCATGCCATTGGCCGAGCCCCTGCACAGCCGCGGTCGtt                                             >  6:236050/1‑149 (MQ=255)
                                                                                             aCCTGGGGGCGGAAACGTCGCCCTGTTCACAAGGTTATCCACAATAAATGTGGATAACCTTTTCCTCTCGGAGGCTCCATGAGCGCACCCTGGAATTTTGCCCGTTTCCTGCCATTGGCCGAGCGCCTGCTCAGCCGCGGTCGTTTgtc                                         <  4:6293/149‑3 (MQ=255)
                                                                                                  ggggCGGAAACGTCGCCCTGTTCACAAGGTTATCCACAATAAATGTGGATAACCTTTTCCTCTCGGAGGCTCCATGAGCGCACCCTGGAATTTTGCCCGTTTCCTGCCATTGGCCGAGCGCCTGCTCAGCCGCGGTCGTTTGCCGGcc                                     <  4:239688/148‑1 (MQ=255)
                                                                                                     gCGGAAACGTCGCCCTGTTCACAAGGTTATCCACAATAAATGTGGATAACCTTTTCCTCTCGGAGGCTCCATGAGCGCACCCTGGAATTTTGCCCGTTTCCTGCCATTGGCCGAGCGCCTGCTCAGCCGCGGTCGTTTGCCGGCCttgt                                 <  3:274120/149‑1 (MQ=255)
                                                                                                        gAAACGTCGCCCTGTTCACAAGGTTATCCACAATAAATGTGGATAACCTTTTCCTCTCGGAGGCTCCATGAGCGCACCCTGGAATTTTGCCCGTTTCCTGCCATTGGCCGAGCGCCTGCTCAGCCGTGGTCGTTTGCCGGCCttg                                  <  7:116675/145‑1 (MQ=255)
                                                                                                        gAAACGTCGCCCTGTTCACAAGGTTATCCACAATAAATGTGGATAACCTTTTCCTCTCGGAGGCTCCATGAGCGCACCCTGGAATTTTGCCCGTTTCCTGCCATTGGCCGAGCGCCTGCTCAGCCGTGGTCGTTTGCCGGCCttg                                  >  8:116675/1‑145 (MQ=255)
                                                                                                        gAAACGTCGCCCTGTTCACAAGGTTATCCACAATAAATGTGGATAACCTTTTCCTCTCGGAGGCTCCATGAGCGCACCCTGGAATTTTGCCCGTTTCCTGCCATTGGCCGAGCGCCTGCTCAGCCGCGGTCGTTTGCCGGCCTtgttgt                              >  4:327503/1‑149 (MQ=255)
                                                                                                         aaaCGTCGCCCTGTTCACAAGGTTATCCACAATAAATGTGGATAACCTTTTCCTCTCGGAGGCTCCATGAGCGCACCCTGGAATTTTGCCCGTTTCCTGCCATTGGCCGAGCGCCTGCTCAGCCGCGGTCGTTTGCCGGCCTTgttgtt                             >  7:213280/1‑149 (MQ=255)
                                                                                                             gTCGCCCTGTTCACAAGGTTATCCACAATAAATGTGGATAACCTTTTCCTCTCGGAGGCTCCATGAGCGCACCCTGGAATTTTGCCCGTTTCCTGCCATTGGCCGAGCGCCTGCTCAGCCGCGGTCGTTTGCCGGCCTTGTTGTTcgcg                         >  6:289174/1‑149 (MQ=255)
                                                                                                                gCCCTGTTCACAAGGTTATCCACAATAAATGTGGATAACCTTTTCCTCTCGGAGGCTCCATGAGCGCACCCTGGAATTTTGCCCGTTTCCTGCCATTGGCCGAGCGCCTGCTCAGCCGCGGTCGTTTGCCGGCCTTGTTGTTCGCGGt                       <  5:236050/148‑1 (MQ=255)
                                                                                                                 cccTGTTCACAAGGTTATCCACAATAAATGTGGATAACCTTTTCCTCTCGGAGGCTCCATGAGCGCACCCTGGAATTTTGCCCGTTTCCTGCCATTGGCCGAGCGCCt                                                              >  3:121472/1‑108 (MQ=255)
                                                                                                                 cccTGTTCACAAGGTTATCCACAATAAATGTGGATAACCTTTTCCTCTCGGAGGCTCCATGAGCGCACCCTGGAATTTTGCCCGTTTCCTGCCATTGGCCGAGCGCCt                                                              <  4:121472/108‑1 (MQ=255)
                                                                                                                         acaAGGTTATCCACAATAAATGTGGATAACCTTTTCCTCTCGGAGGCTCCATGAGCGCACCCTGGAATTTTGCCCGTTTCCTGCCATTGGCCGAGCGCCTGCTCAGCCGCGGTCGTTTGCCGGCCTTGTTGTTCGCGGTGGCGCGCa               >  4:176576/1‑147 (MQ=255)
                                                                                                                             ggTTATCCACATTTATTGTGGATAACCTTTTCCTCTCGGAGGCTCCATGAGCGCACCCTGGAATTTTGCCCGTTTCCTGCCATTGGCCGAGCGCCTGCTCAGCCGCGGTCGTTTGCCGGCCTTGTTGTTCGCGGTGGCGCGCAAAGGcc         >  8:164769/1‑149 (MQ=255)
                                                                                                                                    cacaATAAATGTGGATAACCTTTTCCTCTCGGAGGCTCCATGAGCGCACCCTGGAATTTTGCCCGTTTCCTGCCATTGGCCGAGCGCCTGCTCAGCCGCGGTCGTTTGCCGGCCTTGTTGTTCGCGGTGGCGCGCAAAGGCCCGCGCCt  >  7:282051/1‑149 (MQ=255)
                                                                                                                                        |                                                                                                                                                
TTCTGACGCAAATCGTTTGCGCCGCAGGTAGTCATGGCGTGAAACGACTGTGTAAAAAACGCCCGATCTGACCGGGCCCCTTGCCTGGCAGGCACCTGGGGGCGGAAACGTCGCCCTGTTCACAAGGTTATCCACAATAAATGTGGATAACCTTTTCCTCTCGGAGGCTCCATGAGCGCACCCTGGAATTTTGCCCGTTTCCTGCCATTGGCCGAGCGCCTGCTCAGCCGCGGTCGTTTGCCGGCCTTGTTGTTCGCGGTGGCGCGCAAAGGCCCGCGCCT  >  NC_002947/1915690‑1915970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑