Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,923,100 G→A 8.3% C48C (TGC→TGT)  PP_1722 ← ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,923,1000GA 8.3% 137.3 / 2.3 48C48C (TGC→TGT) PP_1722ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (21/23);  new base A (2/2);  total (23/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

AGGCGACATTCTGCTCCACGGTCATGTTAGGGAACAGCGCGTAGCTCTGGAACACCATGCCGATGCCACGCTTTTGCGGGCTTAGCGGCACGATGTCGTGGCCGTCGAGCAGTATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATGCAGCGCAGCAGGGTGGACTTGCCGCAGCCGGACGGGCCGAGCAGGGTGACGAATTCGCCGCGTTCGATGTGGCAGTCGATGCGCTCGAACACCGGGCTGCCGGCGTAGCTTTTCTGCAGTTTCTGTACGCTGACGAAGCTCATGTCAG  >  NC_002947/1922952‑1923248
                                                                                                                                                    |                                                                                                                                                    
aGGCGACATTCTGCTCCACGGTCATGTTAGGGAACAGCGCGTAGCTCTGGAACACCATGCCGATGCCACGCTTTTGCGGGCTTAGCGGCACGATGTCGTGGCCGTCGAGCAGTATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATg                                                                                                                                                      <  1:155823/149‑1 (MQ=255)
     aCATTCTGCTCCACGGTCATGTTAGGGAACAGCGCGTAGCTCTGGAACACCATGCCGATGCCACGCTTTTGCGGGCTTAGCGGCACGATGTCGTGGCCGTCGAGCAGTATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATgcagcg                                                                                                                                                 >  6:129/1‑149 (MQ=255)
         tCTGCTCCACGGTCATGTTAGGGAACAGCGCGTAGCTCTGGAACACCATGCCGATGCCACGCTTTTGCGGGCTTATCGGCACGATGTCGTGGCCGTCGAGCAGTATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATgcagc                                                                                                                                                  <  4:324841/144‑1 (MQ=255)
         tCTGCTCCACGGTCATGTTAGGGAACAGCGCGTAGCTCTGGAACACCATGCCGATGCCACGCTTTTGCGGGCTTAGCGGCACGATGTCGTGGCCGTCGAGCAGTATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATgcagc                                                                                                                                                  >  3:324841/1‑144 (MQ=255)
              tCCACGGTCATGTTAGGGAACAGCGCGTAGCTCTGGAACACCATGCCGATGCCACGCTTTTGCGGGCTTAGCGGCACGATGTCGTGGCCGTCGAGCAGTATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATgca                                                                                                                                                    >  5:76655/1‑137 (MQ=255)
              tCCACGGTCATGTTAGGGAACAGCGCGTAGCTCTGGAACACCATGCCGATGCCACGCTTTTGCGGGCTTAGCGGCACGATGTCGTGGCCGTCGAGCAGTATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATgca                                                                                                                                                    <  6:76655/137‑1 (MQ=255)
                cACGGTCATGTTAGGGAACAGCGCGTAGCTCTGGAACACCATGCCGATGCCACGCTTTTGCGGGCTTAGCGGCACGATGTCGTGGCCGTCGAGCAGTATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATGCAGCGCAGCAGGGt                                                                                                                                        <  1:335182/147‑1 (MQ=255)
                 aCGGTCATGTTAGGGAACAGCGCGTAGCTCTGTAACACCATGCCGATGCCACGCTTTTGCGGGCTTAGCGGCACGATGTCGTGGCCGTCGAGCAGTATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATGCAGCGCAGCAGGGTgg                                                                                                                                      >  3:114403/1‑148 (MQ=255)
                 aCGGTCATGTTAGGGAACAGCGCGTAGCTCTGTAACACCATGCCGATGCCACGCTTTTGCGGGCTTAGCGGCACGATGTCGTGGCCGTCGAGCAGTATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATGCAGCGCAGCAGGGTGGa                                                                                                                                     >  3:119275/1‑149 (MQ=255)
                        tGTTAGGGAACAGCGCGTAGCTCTGGAACACCATGCCGATGCCACGCTTTTGCGGGCTTAGCGGCACGATGTCGTGGCCGTCGAGCAGTATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATGCAGCGCAGCAGGGTGGACTTGCCg                                                                                                                              <  5:129/149‑1 (MQ=255)
                             gggAACAGCGCGTAGCTCTGGAACACCATGCCGATGCCACGCTTTTGCGGGCTTAGCGGCACGATGTCGTGGCCGTCGAGCAGTATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATGCAGCGCAGCAGGGTGGACTTGCCGCAGcc                                                                                                                         >  8:249774/1‑149 (MQ=255)
                              ggAACAGCGCGTAGCTCTGGAACACCATGCCGATGCCACGCTTTTGCGGGCTTAGCGGCACGATGTCGTGGCCGTCGAGCAGTATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATGCAGCGCAGCAGGGTGGACTTGCCGCAGCCg                                                                                                                        <  2:210967/149‑1 (MQ=255)
                              ggAACAGCGCGTAGCTCTGGAACACCATGCCGATGCCACGCTTTTGCGGGCTTAGCGGCACGATGTCGTGGCCGTCGAGCAGTATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATGCAGCGCAGCAGGGTGGACTTGCCGCAGCCg                                                                                                                        <  2:205887/149‑1 (MQ=255)
                                 aCAGCGCGTAGCTCTGGAACACCATGCCGATGCCACGCTTTTGCGGGCTTAGCGGCACGATGTCGTGGCCGTCGAGCAGTATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATGCAGCGCAGCAGGGTGGACTTGCCGCAGCCGGAc                                                                                                                     >  8:35900/1‑149 (MQ=255)
                                               tGGAACACCATGCCGATGCCACGCTTTTGCGGGCTTAGCGGCACGATGTCGTGGCCGTCGAGCAGTATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATGCAGCGCAGCAGGGTGGACTTGCCGCAGCCGGACGGGCCGAGCAgg                                                                                                         <  7:35900/147‑1 (MQ=255)
                                                       cATGCCGATGCCACGCTTTTGCGGGCTTAGCGGCACGATGTCGTGGCCGTCGAGCAGTATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATGCAGCGCAGCAGGGTGGACTTGCCGCAGCCGGACGGGCCGAg                                                                                                             >  3:184735/1‑135 (MQ=255)
                                                       cATGCCGATGCCACGCTTTTGCGGGCTTAGCGGCACGATGTCGTGGCCGTCGAGCAGTATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATGCAGCGCAGCAGGGTGGACTTGCCGCAGCCGGACGGGCCGAg                                                                                                             <  4:184735/135‑1 (MQ=255)
                                                       cATGCCGATGCCACGCTTTTGCGGGCTTAGCGGCACGATGTCGTGGCCGTCGAGCAGTATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATGCAGCGCAGCAGGGAGGACTTGCCGCAGCCGGACGGGCCGAGCAGGGTGACGAAtt                                                                                               >  7:167908/1‑149 (MQ=255)
                                                        aTGCCGATGCCACGCTTTTGCGGGCTTAGCGGCACGATGTCGTGGCCGTCGAGCAGTATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATGCAGCGCAGCAGGGTGGACTTGCCGCAGCCGGACGGGCCGAGCAGGGTGACGAATTc                                                                                              >  4:238066/1‑149 (MQ=255)
                                                         tGCCGATGCCACGCTTTTGCGGGCTTAGCGGCACGATGTCGTGGCCGTCGAGCAGTATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATGCAGCGCAGCAGGGTGGACTTGCCGCAGCCGGACGGGCCGAGCAGGGTGACGAATTcg                                                                                             <  2:72208/149‑1 (MQ=255)
                                                           ccGATGCCACGCTTTTGCGGGCTTAGCGGCACGATGTCGTGGCCGTCGAGCAGTATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATGCAGCGCAGCAGGGTGGACTTGCCGCAGCCGGACGGGCCGAGCAGGGTGACGAATTcgcc                                                                                           <  7:249774/149‑1 (MQ=255)
                                                                  cACGCTTTTGCGGGCTTAGCGGCACGATGTCGTGGCCGTCGAGCAGTATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATGCAGCGCAGCAGGGTGGACTTGCCGCAGCCGGACGGGCCGAGCAGGGTGACGAATTCGCCGCGTTc                                                                                     >  6:355114/1‑148 (MQ=255)
                                                                    cGCTTTTGCGGGCTTAGCGGCACGATGTCGTGGCCGTCGAGCAGTATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATGCAGCGCAGCAGGGTGGACTTGCCGCAGCCGGACGGGCCGAGCAGGGTGACGAATTCGCCGCGTTCGAt                                                                                  <  3:238066/149‑1 (MQ=255)
                                                                     gCTTTTGCGGGCTTAGCGGCACGATGTCGTGGCCGTCGAGCAGTATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATGCAGCGCAGCAGGGTGGACTTGCCGCAGCCGGACGGGCCGAGCAGGGTGACGAATTCGCCGCGTTCGa                                                                                   <  2:21614/147‑1 (MQ=255)
                                                                     gCTTTTGCGGGCTTAGCGGCACGATGTCGTGGCCGTCGAGCAGTATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATGCAGCGCAGCAGGGTGGACTTGCCGCAGCCGGACGGGCCGAGCAGGGTGACGAATTCGCCGCGTTCGa                                                                                   >  1:21614/1‑147 (MQ=255)
                                                                            cGGGCTTAGCGGCACGATGTCGTGGCCGTCGAGCAGTATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATGCAGCGCAGCAGGGAGGACTTGCCGCAGCCGGACGGGCCGAGCAGGGTGACGAATTCGCCGCGTTCGATGTGGCAg                                                                           <  8:167908/148‑1 (MQ=255)
                                                                               gCTTAGCGGCACGATGTCGTGGCCGTCGAGCAGTATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATGCAGCGCAGCAGGGTGGACTTGCCGCAGCCGGACGGGCCGAGCAGGGGGACGAATTCGCCGCGTTCGATGTGGCAGt                                                                          <  8:231203/146‑1 (MQ=255)
                                                                                   aGCGGCACGATGTCGTGGCCGTCGAGCAGTATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATGCAGCGCAGCAGGGTGGACTTGCCGCAGCCGGACGGGCCGAGCAGGGTGACGAATTCGCCGCGtt                                                                                      >  6:308763/1‑130 (MQ=255)
                                                                                   aGCGGCACGATGTCGTGGCCGTCGAGCAGTATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATGCAGCGCAGCAGGGTGGACTTGCCGCAGCCGGACGGGCCGAGCAGGGTGACGAATTCGCCGCGtt                                                                                      <  5:308763/130‑1 (MQ=255)
                                                                                                       gTCGAGCAGTATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATACAGCGCAGCAGGGTGGACTTGCCGCAGCCGGACGGGCCGAGCAGGGTGACGAATTCGCCGCGTTCGATGTGGCAGTCGATGCGCTCGAACACCGGGCTGcc                                                 >  1:154286/1‑147 (MQ=255)
                                                                                                       gTCGAGCAGTATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATACAGCGCAGCAGGGTGGACTTGCCGCAGCCGGACGGGCCGAGCAGGGTGACGAATTCGCCGCGTTCGATGTGGCAGTCGATGCGCTCGAACACCGGGCTGcc                                                 <  2:159475/147‑1 (MQ=255)
                                                                                                       gTCGAGCAGTATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATACAGCGCAGCAGGGTGGACTTGCCGCAGCCGGACGGGCCGAGCAGGGTGACGAATTCGCCGCGTTCGATGTGGCAGTCGATGCGCTCGAACACCGGGCTGcc                                                 <  2:154286/147‑1 (MQ=255)
                                                                                                       gTCGAGCAGTATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATACAGCGCAGCAGGGTGGACTTGCCGCAGCCGGACGGGCCGAGCAGGGTGACGAATTCGCCGCGTTCGATGTGGCAGTCGATGCGCTCGAACACCGGGCTGcc                                                 >  1:159475/1‑147 (MQ=255)
                                                                                                             cAGTATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATGCAGCGCAGCAGGGTGGACTTGCCGCAGCCGGACGGGCCGAGCAGGGTGACGAATTCGCCGCGTTCGATGTGGCAGTCGATGCGCTCGAACAcc                                                         >  8:326664/1‑133 (MQ=255)
                                                                                                             cAGTATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATGCAGCGCAGCAGGGTGGACTTGCCGCAGCCGGACGGGCCGAGCAGGGTGACGAATTCGCCGCGTTCGATGTGGCAGTCGATGCGCTCGAACAcc                                                         <  7:326664/133‑1 (MQ=255)
                                                                                                               gTATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATGCAGCGCAGCAGGGTGGACTTGCCGCAGCCGGACGGGCCGAGCAGGGTGACGAATTCGCCGCGTTCGATGTGGCAGTCGAt                                                                      <  7:68804/118‑1 (MQ=255)
                                                                                                               gTATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATGCAGCGCAGCAGGGTGGACTTGCCGCAGCCGGACGGGCCGAGCAGGGTGACGAATTCGCCGCGTTCGATGTGGCAGTCGAt                                                                      >  8:68804/1‑118 (MQ=255)
                                                                                                                tATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATGCAGCGCAGCAGGGTGGACTTGCCGCAGCCGGACGGGCCGAGCAGGGTGACGAATTCGCCGCGTTCGATGTGGCAGTCGATGCGCTCGAACACCGGGCTGCCGGCGt                                            >  6:108643/1‑143 (MQ=255)
                                                                                                                tATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATGCAGCGCAGCAGGGTGGACTTGCCGCAGCCGGACGGGCCGAGCAGGGTGACGAATTCGCCGCGTTCGATGTGGCAGTCGATGCGCTCGAACACCGGGCTGCCGGCGt                                            <  5:108643/143‑1 (MQ=255)
                                                                                                                tATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATGCAGCGCAGCAGGGTGGACTTGCCGCAGCCGGACGGGCCGAGCAGGGTGACGAATTCGCCGCGTTCGATGTGGCAGTCGATGCGCTCGAACACCGGGCTGCCGGCGTAGCttt                                      >  1:49731/1‑149 (MQ=255)
                                                                                                                    tGCCCGCTGTCCACCGAGGTCAGCCCGGCAATGCAGCGCAGCAGGGTGGACTTGCCGCAGCCGGACGGGCCGAGCAGGGTGACGAATTCGCCGCGTTCGATGTGGCAGTCGATGCGCTCGAACACCGGGCTGCCGGCGTAGCTTTTCTg                                  >  6:358914/1‑149 (MQ=255)
                                                                                                                      cccGCTGTCCACCGAGGTCAGCCCGGCAATGCAGCGCAGCAGGGTGGACTTGCCGCAGCCGGACGGGCCGAGCAGGGTGACGAATTCGCCGCGTTCGATGTGGCAGTCGATGCGCTCGAACACCGGGCTGCCGGCGTAGCTTTTCTGCa                                <  1:138784/149‑1 (MQ=255)
                                                                                                                             tCCACCGAGGTCAGCCCGGCAATGCAGCGCAGCAGGGTGGACTTGCCGCAGCCGGACGGGCCGAGCAGGGTGACGAATTCGCCGCGTTCGATGTGGCAGTCGATGCGCTCGAACACCGGGCTGCCGGCGTAGCTTTTCTGCAGTTTc                           <  5:358914/147‑1 (MQ=255)
                                                                                                                                aCCGAGGTCAGCCCGGCAATGCAGCGCAGCAGGGTGGACTTGCCGCAGCCGGACGGGCCGAGCAGGGTGACGAATTCGCCGCGTTCGATGTGGCAGTCGATGCGCTCGAACACCGGGCTGCCGGCGTAGCTTTTCTGCAGTTTCTGTAc                      >  4:125848/1‑149 (MQ=255)
                                                                                                                                  cGAGGTCAGCCCGGCAATGCAGCGCAGCAGGGTGGACTTGCCGCAGCCGGACGGGCCGAGCAGGGTGACGAATTCGCCGCGTTCGATGTGGCAGTCGATGCGCTCGAACACCGGGCTGCCGGCGTAGCtttt                                     >  8:184665/1‑132 (MQ=255)
                                                                                                                                  cGAGGTCAGCCCGGCAATGCAGCGCAGCAGGGTGGACTTGCCGCAGCCGGACGGGCCGAGCAGGGTGACGAATTCGCCGCGTTCGATGTGGCAGTCGATGCGCTCGAACACCGGGCTGCCGGCGTAGCtttt                                     <  7:184665/132‑1 (MQ=255)
                                                                                                                                   gAGGTCAGCCCGGCAATGCAGCGCAGCAGGGTGGACTTGCCGCAGCCGGACGGGCCGAGCAGGGTGACGAATTCGCCGCGTTCGATGTgg                                                                              <  8:122797/90‑1 (MQ=255)
                                                                                                                                   gAGGTCAGCCCGGCAATGCAGCGCAGCAGGGTGGACTTGCCGCAGCCGGACGGGCCGAGCAGGGTGACGAATTCGCCGCGTTCGATGTgg                                                                              <  8:122249/90‑1 (MQ=255)
                                                                                                                                           cccGGCAATGCAGCGCAGCAGGGTGGACTTGCCGCAGCCGGACGGGCCGAGCAGGGTGACGAATTCGCCGCGTTCGATGTGGCAGTCGATGCGCTCGAACACCGGGCTGCCGGCGTAGCTTTTCTGCAGTTTCTGTACGCTGa                 >  8:121590/1‑143 (MQ=255)
                                                                                                                                           cccGGCAATGCAGCGCAGCAGGGTGGACTTGCCGCAGCCGGACGGGCCGAGCAGGGTGACGAATTCGCCGCGTTCGATGTGGCAGTCGATGCGCTCGAACACCGGGCTGCCGGCGTAGCTTTTCTGCAGTTTCTGTACGCTGa                 <  7:121590/143‑1 (MQ=255)
                                                                                                                                           cccGGCAATGCAGCGCAGCAGGGTGGACTTGCCGCAGCCGGACGGGCCGAGCAGGGTGACGAATTCGCCGCGTTCGATGTGGCAGTCGATGCGCTCGAACACCGGGCTGCCGGCGTAGCTTTTCTGCAGTTTCTGTACGCTGACGAAGc           >  5:264929/1‑149 (MQ=255)
                                                                                                                                             cGGCAATGCAGCGCAGCAGGGTGGACTTGCCGCAGCCGGACGGGCCGAGc                                                                                                            >  7:332666/1‑50 (MQ=255)
                                                                                                                                             cGGCAATGCAGCGCAGCAGGGTGGACTTGCCGCAGCCGGACGGGCCGAGc                                                                                                            <  8:332666/50‑1 (MQ=255)
                                                                                                                                                 aaTGCAGCGCAGCAGGGTGGACTTGCCGCAGCCGGACGGGCCGAGCAGGGTGACGAATTCGCCGCGTTCGATGTGGCAGTCGATGCGCTCGAACACCGGGCTGCCGGCGTAGCTTTTCTGCAGTTTCTGTACGCTGACGAAGCTCATGt     >  4:343390/1‑149 (MQ=255)
                                                                                                                                                    gcagcgcagcAGGGTGGACTTGCCGCAGCCGGACGGGCCGAGCAGGGTGACGAATTCGCCGCGTTCGATGTGGCAGTCGATGCGCTCGAACACCGGGCTGCCGGCGTAGCTTTTCTGCAGTTTCTGTACGCTGACGAAGCTCATGTCAg  >  7:26927/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AGGCGACATTCTGCTCCACGGTCATGTTAGGGAACAGCGCGTAGCTCTGGAACACCATGCCGATGCCACGCTTTTGCGGGCTTAGCGGCACGATGTCGTGGCCGTCGAGCAGTATCTGCCCGCTGTCCACCGAGGTCAGCCCGGCAATGCAGCGCAGCAGGGTGGACTTGCCGCAGCCGGACGGGCCGAGCAGGGTGACGAATTCGCCGCGTTCGATGTGGCAGTCGATGCGCTCGAACACCGGGCTGCCGGCGTAGCTTTTCTGCAGTTTCTGTACGCTGACGAAGCTCATGTCAG  >  NC_002947/1922952‑1923248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑