Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,938,844 T→C 6.1% E71G (GAG→GGG)  PP_1738 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,938,8440TC 6.1% 190.1 / 2.2 65E71G (GAG→GGG) PP_1738hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (31/30);  new base C (2/2);  total (33/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.73e-01

CTTTTTCAGGTATTCATTGATGCTGCTGCTGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCTGGCAGCGTCGATATC  >  NC_002947/1938696‑1938986
                                                                                                                                                    |                                                                                                                                              
cTTTTTCAGGTATTCATTGATGCTGCTGCTGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCt                                                                                                                                                >  4:346820/1‑149 (MQ=255)
  ttttCAGGTATTCATTGATGCTGCTGCTGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTcc                                                                                                                                              >  2:221117/1‑149 (MQ=255)
               aTTGATGCTGCTGCTGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTTGCGCCCGGAGCGCGAGTTc                                                                                                                                  <  1:254811/148‑1 (MQ=255)
               aTTGATGCTGCTGCTGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTTGCGCCCGGAGCGCGAGTTCa                                                                                                                                 <  5:273064/149‑1 (MQ=255)
               aTTGATGCTGCTGCTGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCa                                                                                                                                 <  8:237443/149‑1 (MQ=255)
               aTTGATGCTGCTGCTGTAGGGCGCGCCGGTGCCGGCGATGATGAAAATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGtt                                                                                                                                   >  1:71791/1‑147 (MQ=255)
                ttGATGCTGCTGCTGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAg                                                                                                                                >  4:90450/1‑149 (MQ=255)
                   aTGCTGCTGCTGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCt                                                                                                                             <  6:124202/149‑1 (MQ=255)
                       tgctgctgTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTTGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAgg                                                                                                                               >  4:265202/1‑143 (MQ=255)
                       tgctgctgTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTTGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAgg                                                                                                                               <  3:265202/143‑1 (MQ=255)
                         ctgctgTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTa                                                                                                                          >  6:209011/1‑146 (MQ=255)
                         ctgctgTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTa                                                                                                                          <  5:209011/146‑1 (MQ=255)
                         ctgctgTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTc                                                                                                                       <  1:205186/149‑1 (MQ=255)
                          tgctgTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCg                                                                                                                      >  7:9912/1‑149 (MQ=255)
                          tgctgTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCg                                                                                                                      >  7:144678/1‑149 (MQ=255)
                           gctgTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTc                                                                                                                       <  6:100399/147‑1 (MQ=255)
                           gctgTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTc                                                                                                                       >  5:100399/1‑147 (MQ=255)
                              gTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACt                                                                                                                  >  4:125709/1‑149 (MQ=255)
                               tAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACt                                                                                                                  <  3:129973/148‑1 (MQ=255)
                                aGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACt                                                                                                                  >  5:171488/1‑147 (MQ=255)
                                 gggCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGa                                                                                                                <  8:9912/148‑1 (MQ=255)
                                 gggCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAg                                                                                                               <  3:90450/149‑1 (MQ=255)
                                      cgcCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGAtt                                                                                                          <  2:194244/149‑1 (MQ=255)
                                          ggTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGAc                                                                                                                   >  4:242508/1‑136 (MQ=255)
                                          ggTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGAc                                                                                                                   <  3:242508/136‑1 (MQ=255)
                                           gTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCAtc                                                                                                     <  3:346820/149‑1 (MQ=255)
                                                   gatgatGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTgg                                                                                             <  6:171488/149‑1 (MQ=255)
                                                   gatgatGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTgg                                                                                             <  3:125709/149‑1 (MQ=255)
                                                         gaAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCt                                                                                       >  8:63461/1‑149 (MQ=255)
                                                         gaAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCt                                                                                       >  8:225638/1‑149 (MQ=255)
                                                           aGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCt                                                                                       >  6:296391/1‑147 (MQ=255)
                                                           aGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCa                                                                                     <  5:296391/149‑1 (MQ=255)
                                                            gATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAg                                                                                    >  7:38648/1‑149 (MQ=255)
                                                            gATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGCCGCCGGACaagcgcc                                                                                    >  7:98027/1‑143 (MQ=255)
                                                             aTCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAgg                                                                                   <  7:63461/149‑1 (MQ=255)
                                                             aTCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAgg                                                                                   <  8:144678/149‑1 (MQ=255)
                                                                 gcggcgCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGgcgc                                                                               >  5:74900/1‑149 (MQ=255)
                                                                   ggcgCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGgcg                                                                                                                                         >  7:304844/1‑89 (MQ=255)
                                                                   ggcgCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGgcg                                                                                                                                         <  8:304844/89‑1 (MQ=255)
                                                                     cgcTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGt                                                                           <  1:221117/149‑1 (MQ=255)
                                                                      gcTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTc                                                                          <  6:74900/149‑1 (MQ=255)
                                                                          cGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCcggc                                                                      >  8:238581/1‑149 (MQ=255)
                                                                             gATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCCCAGGGCGCTTGTCCggcggc                                                                   >  4:316794/1‑149 (MQ=255)
                                                                               tCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCgt                                                                 >  7:44976/1‑149 (MQ=255)
                                                                                 cTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTGCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGTCGCTTGTCCGGCGGCgtgg                                                               >  4:51595/1‑149 (MQ=255)
                                                                                    gCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGtggtgg                                                            >  1:238087/1‑149 (MQ=255)
                                                                                    gCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGtggtgg                                                            >  1:305645/1‑149 (MQ=255)
                                                                                     cGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGc                                                           <  7:238581/149‑1 (MQ=255)
                                                                                      gggCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCg                                                          >  3:43292/1‑149 (MQ=255)
                                                                                        gCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGAt                                                        >  1:80902/1‑149 (MQ=255)
                                                                                         ccAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCtt                                                                             <  7:66771/127‑1 (MQ=255)
                                                                                         ccAGGCGCTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTCGGCAGCTCAGGGCGCtt                                                                             >  8:66771/1‑127 (MQ=255)
                                                                                           aGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGAtggt                                                     <  2:71791/149‑1 (MQ=255)
                                                                                                gTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCCCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCt                                                <  3:316794/149‑1 (MQ=255)
                                                                                                 tACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTc                                               <  2:80902/149‑1 (MQ=255)
                                                                                                         cttcttCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGt                                       <  7:225638/149‑1 (MQ=255)
                                                                                                                   gCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGtggtgg                                                            >  6:349230/1‑118 (MQ=255)
                                                                                                                   gCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGtggtgg                                                            <  5:349230/118‑1 (MQ=255)
                                                                                                                     ccAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACggg                                        <  3:342665/136‑1 (MQ=255)
                                                                                                                     ccAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACggg                                        >  4:342665/1‑136 (MQ=255)
                                                                                                                                 gggCAATGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGGGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCTgg               >  7:316857/1‑149 (MQ=255)
                                                                                                                                    cAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCTGGCAg            <  8:44976/149‑1 (MQ=255)
                                                                                                                                         tGAACTTGCGCCCGGAGCGCGAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCTGGCAGCGTc        >  4:313523/1‑148 (MQ=255)
                                                                                                                                          gAACTTGCGCCCGGAGCGCGAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCTGGCAGCGTCGa      >  4:114900/1‑149 (MQ=255)
                                                                                                                                           aaCTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCTGGCAGCGTCGat     <  8:38648/149‑1 (MQ=255)
                                                                                                                                              tCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCTGGCAGCGTCGATATc  >  1:55798/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
CTTTTTCAGGTATTCATTGATGCTGCTGCTGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCTGGCAGCGTCGATATC  >  NC_002947/1938696‑1938986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑