Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,938,846 C→G 6.2% P70P (CCG→CCC)  PP_1738 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,938,8460CG 6.2% 204.5 / 4.0 64P70P (CCG→CCC) PP_1738hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (30/30);  new base G (2/2);  total (32/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.71e-01

TTTTCAGGTATTCATTGATGCTGCTGCTGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCTGGCAGCGTCGATATCCCGCGCTG  >  NC_002947/1938698‑1938994
                                                                                                                                                    |                                                                                                                                                    
ttttCAGGTATTCATTGATGCTGCTGCTGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTcc                                                                                                                                                      >  2:221117/1‑149 (MQ=255)
             aTTGATGCTGCTGCTGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTTGCGCCCGGAGCGCGAGTTc                                                                                                                                          <  1:254811/148‑1 (MQ=255)
             aTTGATGCTGCTGCTGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTTGCGCCCGGAGCGCGAGTTCa                                                                                                                                         <  5:273064/149‑1 (MQ=255)
             aTTGATGCTGCTGCTGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCa                                                                                                                                         <  8:237443/149‑1 (MQ=255)
             aTTGATGCTGCTGCTGTAGGGCGCGCCGGTGCCGGCGATGATGAAAATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGtt                                                                                                                                           >  1:71791/1‑147 (MQ=255)
              ttGATGCTGCTGCTGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAg                                                                                                                                        >  4:90450/1‑149 (MQ=255)
                 aTGCTGCTGCTGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCt                                                                                                                                     <  6:124202/149‑1 (MQ=255)
                     tgctgctgTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTTGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAgg                                                                                                                                       >  4:265202/1‑143 (MQ=255)
                     tgctgctgTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTTGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAgg                                                                                                                                       <  3:265202/143‑1 (MQ=255)
                       ctgctgTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTa                                                                                                                                  >  6:209011/1‑146 (MQ=255)
                       ctgctgTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTa                                                                                                                                  <  5:209011/146‑1 (MQ=255)
                       ctgctgTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTc                                                                                                                               <  1:205186/149‑1 (MQ=255)
                        tgctgTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCg                                                                                                                              >  7:9912/1‑149 (MQ=255)
                        tgctgTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCg                                                                                                                              >  7:144678/1‑149 (MQ=255)
                         gctgTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTc                                                                                                                               <  6:100399/147‑1 (MQ=255)
                         gctgTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTc                                                                                                                               >  5:100399/1‑147 (MQ=255)
                            gTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACt                                                                                                                          >  4:125709/1‑149 (MQ=255)
                             tAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACt                                                                                                                          <  3:129973/148‑1 (MQ=255)
                              aGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACt                                                                                                                          >  5:171488/1‑147 (MQ=255)
                               gggCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGa                                                                                                                        <  8:9912/148‑1 (MQ=255)
                               gggCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAg                                                                                                                       <  3:90450/149‑1 (MQ=255)
                                    cgcCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGAtt                                                                                                                  <  2:194244/149‑1 (MQ=255)
                                        ggTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGAc                                                                                                                           >  4:242508/1‑136 (MQ=255)
                                        ggTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGAc                                                                                                                           <  3:242508/136‑1 (MQ=255)
                                         gTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCAtc                                                                                                             <  3:346820/149‑1 (MQ=255)
                                                 gatgatGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTgg                                                                                                     <  6:171488/149‑1 (MQ=255)
                                                 gatgatGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTgg                                                                                                     <  3:125709/149‑1 (MQ=255)
                                                       gaAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCt                                                                                               >  8:63461/1‑149 (MQ=255)
                                                       gaAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCt                                                                                               >  8:225638/1‑149 (MQ=255)
                                                         aGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCt                                                                                               >  6:296391/1‑147 (MQ=255)
                                                         aGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCa                                                                                             <  5:296391/149‑1 (MQ=255)
                                                          gATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAg                                                                                            >  7:38648/1‑149 (MQ=255)
                                                          gATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGCCGCCGGACaagcgcc                                                                                            >  7:98027/1‑143 (MQ=255)
                                                           aTCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAgg                                                                                           <  7:63461/149‑1 (MQ=255)
                                                           aTCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAgg                                                                                           <  8:144678/149‑1 (MQ=255)
                                                               gcggcgCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGgcgc                                                                                       >  5:74900/1‑149 (MQ=255)
                                                                 ggcgCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGgcg                                                                                                                                                 >  7:304844/1‑89 (MQ=255)
                                                                 ggcgCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGgcg                                                                                                                                                 <  8:304844/89‑1 (MQ=255)
                                                                   cgcTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGt                                                                                   <  1:221117/149‑1 (MQ=255)
                                                                    gcTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTc                                                                                  <  6:74900/149‑1 (MQ=255)
                                                                        cGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCcggc                                                                              >  8:238581/1‑149 (MQ=255)
                                                                           gATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCCCAGGGCGCTTGTCCggcggc                                                                           >  4:316794/1‑149 (MQ=255)
                                                                             tCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCgt                                                                         >  7:44976/1‑149 (MQ=255)
                                                                               cTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTGCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGTCGCTTGTCCGGCGGCgtgg                                                                       >  4:51595/1‑149 (MQ=255)
                                                                                  gCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGtggtgg                                                                    >  1:305645/1‑149 (MQ=255)
                                                                                  gCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGtggtgg                                                                    >  1:238087/1‑149 (MQ=255)
                                                                                   cGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGc                                                                   <  7:238581/149‑1 (MQ=255)
                                                                                    gggCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCg                                                                  >  3:43292/1‑149 (MQ=255)
                                                                                      gCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGAt                                                                >  1:80902/1‑149 (MQ=255)
                                                                                       ccAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCtt                                                                                     <  7:66771/127‑1 (MQ=255)
                                                                                       ccAGGCGCTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTCGGCAGCTCAGGGCGCtt                                                                                     >  8:66771/1‑127 (MQ=255)
                                                                                         aGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGAtggt                                                             <  2:71791/149‑1 (MQ=255)
                                                                                              gTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCCCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCt                                                        <  3:316794/149‑1 (MQ=255)
                                                                                               tACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTc                                                       <  2:80902/149‑1 (MQ=255)
                                                                                                       cttcttCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGt                                               <  7:225638/149‑1 (MQ=255)
                                                                                                                 gCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGtggtgg                                                                    <  5:349230/118‑1 (MQ=255)
                                                                                                                 gCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGtggtgg                                                                    >  6:349230/1‑118 (MQ=255)
                                                                                                                   ccAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACggg                                                >  4:342665/1‑136 (MQ=255)
                                                                                                                   ccAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACggg                                                <  3:342665/136‑1 (MQ=255)
                                                                                                                               gggCAATGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGGGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCTgg                       >  7:316857/1‑149 (MQ=255)
                                                                                                                                  cAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCTGGCAg                    <  8:44976/149‑1 (MQ=255)
                                                                                                                                       tGAACTTGCGCCCGGAGCGCGAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCTGGCAGCGTc                >  4:313523/1‑148 (MQ=255)
                                                                                                                                        gAACTTGCGCCCGGAGCGCGAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCTGGCAGCGTCGa              >  4:114900/1‑149 (MQ=255)
                                                                                                                                         aaCTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCTGGCAGCGTCGat             <  8:38648/149‑1 (MQ=255)
                                                                                                                                            tCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCTGGCAGCGTCGATATc          >  1:55798/1‑149 (MQ=255)
                                                                                                                                                    cGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCTGGCAGCGTCGATATCCCGCGCTg  <  2:305645/149‑1 (MQ=255)
                                                                                                                                                    cGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCTGGCAGCGTCGATATCCCGCGCTg  <  2:238087/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TTTTCAGGTATTCATTGATGCTGCTGCTGTAGGGCGCGCCGGTGCCGGCGATGATGAAGATCAGCGGCGCTTCGTGATCCTGGCGGGCCAGGCGGTACTTGAGCTTCTTCACTGCCCAGAAGTTGTCGGGCAACGTGAACTCGCGCTCCGGGCGCAAGTTCAGGCTGTAGTCGGACTGAGTGATTTCATCGTCGCTGGGCAGCTCAGGGCGCTTGTCCGGCGGCGTGGTGGCGATGGTGGCCTCGAACGGGTTGGTAAGAGGGTAGCCATAGCTGGCAGCGTCGATATCCCGCGCTG  >  NC_002947/1938698‑1938994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑