Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,948,173 C→G 10.4% R494R (CGG→CGC)  PP_1749 ← acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,948,1730CG10.4% 138.9 / 7.1 48R494R (CGG→CGC) PP_1749acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (19/24);  new base G (3/2);  total (22/26)
Fisher's exact test for biased strand distribution p-value = 6.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

TGCCCGGCATCACGCACCATGAAGTCCAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGCGCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAGCCTGGCGTCGACGGCTCTGCGCTTCGATCAAC  >  NC_002947/1948027‑1948320
                                                                                                                                                  |                                                                                                                                                   
tGCCCGGCATCACGCACCATGAAGTCCAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGCGCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGcgccg                                                                                                                                                   >  1:20089/1‑149 (MQ=255)
   ccGGCATCACGCACCATGAAGTCCAGACCCACCACCGGAATTTCCAGTGCCCGTGCCGCGCGCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCAGGTGTGCAGGTAGGCCGTGCGCCGCAc                                                                                                                                                >  4:35233/1‑149 (MQ=255)
      gCATCACGCACCATGAAGTCCAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGCGCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGc                                                                                                                                             >  2:340002/1‑149 (MQ=255)
           aCGCACCATGAAGTCCAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGCGCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGgc                                                                                                                                        >  7:215680/1‑149 (MQ=255)
               aCCATGAAGTCCAGGCCCACCACCGGACTTTCCAGTGCCCGTGCCGCGCGCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGGCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTg                                                                                                                                    <  3:35233/149‑1 (MQ=255)
               aCCATGAAGTCCAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGCGCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTg                                                                                                                                    <  4:61650/149‑1 (MQ=255)
                 cATGAAGTCCAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGCGCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGcgccgc                                                                                                                                                  <  6:85899/133‑1 (MQ=255)
                 cATGAAGTCCAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGCGCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGcgccgc                                                                                                                                                  >  5:85899/1‑133 (MQ=255)
                 cATGAAGTCCAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGCGCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTggc                                                                                                                                  >  1:342878/1‑149 (MQ=255)
                   tGAAGTCCAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGCGCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACg                                                                                                                                               >  2:130766/1‑134 (MQ=255)
                   tGAAGTCCAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGCGCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACg                                                                                                                                               <  1:130766/134‑1 (MQ=255)
                     aaGTCCAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGCGCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTggccggc                                                                                                                              <  8:109319/149‑1 (MQ=255)
                      aGTCCAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGCGCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTggccggcc                                                                                                                             <  1:340002/149‑1 (MQ=255)
                      aGTCCAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGCGCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTggccggcc                                                                                                                             <  4:299950/149‑1 (MQ=255)
                        tCCAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGCGCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGccggccgg                                                                                                                           >  4:336372/1‑149 (MQ=255)
                            gCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGCGCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAac                                                                                                                       <  3:338156/149‑1 (MQ=255)
                            gCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGCGCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAac                                                                                                                       <  5:294984/149‑1 (MQ=255)
                                        gAATTTCCAGTGCCCGTGCCGCGCGCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCa                                                                                                              >  2:318370/1‑146 (MQ=255)
                                        gAATTTCCAGTGCCCGTGCCGCGCGCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATa                                                                                                            <  1:318370/148‑1 (MQ=255)
                                        gAATTTCCAGTGCCCGTGCCGCGCGCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAg                                                                                                           <  2:328999/149‑1 (MQ=255)
                                                       gTGCCGCGCGCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCCCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACAcgtcg                                                                                                                 <  3:200272/128‑1 (MQ=255)
                                                       gTGCCGCGCGCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCCCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACAcgtcg                                                                                                                 >  4:200272/1‑128 (MQ=255)
                                                             cgcgcACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAg                                                                                      >  2:209994/1‑149 (MQ=255)
                                                               cgcACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTgcgcaccttgc                                                                                    <  4:117059/149‑8 (MQ=255)
                                                                 cACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTgc                                                                                  >  6:19021/1‑149 (MQ=255)
                                                                   cGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTgcgc                                                                                <  1:88480/149‑1 (MQ=255)
                                                                         caTCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTc                                                                              >  6:224959/1‑145 (MQ=255)
                                                                         caTCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTc                                                                              <  5:224959/145‑1 (MQ=255)
                                                                           tCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTtc                                                                        <  3:336372/149‑1 (MQ=255)
                                                                              gCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTtcgtc                                                                     >  2:306576/1‑149 (MQ=255)
                                                                                cAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTGGTCATAGCCAACGCCCGCTGCGCGCAAGGt                                                                                    <  1:29838/132‑1 (MQ=255)
                                                                                cAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGt                                                                                    >  2:29838/1‑132 (MQ=255)
                                                                                cAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTcgtcgt                                                                   <  3:324118/149‑1 (MQ=255)
                                                                                      cGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCgg                                                                            <  8:130317/134‑1 (MQ=255)
                                                                                      cGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCgg                                                                            >  7:130317/1‑134 (MQ=255)
                                                                                        ggTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGa                                                           <  8:215680/149‑1 (MQ=255)
                                                                                           ctaGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGAAt                                                         <  3:39925/146‑1 (MQ=255)
                                                                                              ggCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGAAt                                                         >  4:39925/1‑145 (MQ=255)
                                                                                                 gTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTtcgtcg                                                                    <  3:238009/131‑1 (MQ=255)
                                                                                                 gTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTtcgtcg                                                                    >  4:238009/1‑131 (MQ=255)
                                                                                                         aCGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCAc                                           <  2:342878/148‑1 (MQ=255)
                                                                                                           gTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCgg                                        <  5:19021/149‑1 (MQ=255)
                                                                                                                    ggTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAGCCt                               <  1:209994/149‑1 (MQ=255)
                                                                                                                              gtgtGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAGCCTGGCGTCGACg                     >  5:359581/1‑149 (MQ=255)
                                                                                                                               tgtgCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCAGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGAGCGCTCGGTATCGTCGTCCAGAAGAATGAGGATATCGACACAGGAAGCAACCTAGCgaagacag                    >  4:196792/1‑142 (MQ=255)
                                                                                                                                   gcgcctGGCCGTGCGGCGCCCGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCCCCCGTg                                      >  3:190109/6‑127 (MQ=255)
                                                                                                                                       ctGGCCGTGCGGCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAGCCTg                              >  5:117179/2‑131 (MQ=255)
                                                                                                                                       ctGGCCGTGCGGCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAGCCTg                              <  6:117179/130‑1 (MQ=255)
                                                                                                                                        tGGCCGTGCGGCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCgg                                                                            >  4:279804/1‑84 (MQ=255)
                                                                                                                                        tGGCCGTGCGGCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCgg                                                                            <  3:279804/84‑1 (MQ=255)
                                                                                                                                         ggCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAGCCTGGCGTCGACGGCTCTGCGCtt          >  8:147500/1‑149 (MQ=255)
                                                                                                                                                 gccgcACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAGCCTGGCGTCGACGGCTCTGCGCTTCGATCAAc  <  3:196792/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TGCCCGGCATCACGCACCATGAAGTCCAGCCCCACCACCGGAATTTCCAGTGCCCGTGCCGCGCGCACGGCAGCATCGGCCAGCACCGGGTGCAGGCGTTCGGTGACGTCCTCCAGGGTGCCGCCGGTGTGCAGGTTGGCCGTGCGCCGCACGGCCAGGCGCTGGCCGGCCGGCAACACGTCGTCATAGCCAACGCCCGCTGCGCGCAAGGTGCGCTCGGTTTCGTCGTCCAGCGGAATGCGGCTCTCGCCACCGGTGGCAGCCTGGCGTCGACGGCTCTGCGCTTCGATCAAC  >  NC_002947/1948027‑1948320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑